Escherichia phage vB_EcoM_4HA13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Chaseviridae; Cleopatravirinae; Sabourvirus; Escherichia virus 4HA13

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D0N8U8|A0A7D0N8U8_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_4HA13 OX=2601675 GN=AC4HA13_640 PE=4 SV=1
MM1 pKa = 7.69LEE3 pKa = 5.24LYY5 pKa = 10.39SQLLSHH11 pKa = 7.14LNAEE15 pKa = 4.48EE16 pKa = 4.04NDD18 pKa = 3.45MALEE22 pKa = 4.21FEE24 pKa = 4.65AKK26 pKa = 10.32LIEE29 pKa = 4.82LLSQQDD35 pKa = 3.59QLNEE39 pKa = 3.87DD40 pKa = 3.92SQFLQCLKK48 pKa = 10.79NAGVDD53 pKa = 3.34DD54 pKa = 3.77WDD56 pKa = 4.68GYY58 pKa = 11.56SFAQEE63 pKa = 4.12EE64 pKa = 4.69MWPEE68 pKa = 4.36DD69 pKa = 3.94DD70 pKa = 6.54DD71 pKa = 4.18EE72 pKa = 4.51

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D0ND75|A0A7D0ND75_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_4HA13 OX=2601675 GN=AC4HA13_170 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 9.7PNYY5 pKa = 9.58VAIRR9 pKa = 11.84KK10 pKa = 9.35SKK12 pKa = 9.59EE13 pKa = 3.62AMFHH17 pKa = 6.3RR18 pKa = 11.84FIEE21 pKa = 4.51AKK23 pKa = 10.2RR24 pKa = 11.84KK25 pKa = 10.08AEE27 pKa = 4.0LEE29 pKa = 4.08GKK31 pKa = 9.79VVIKK35 pKa = 10.48KK36 pKa = 9.85KK37 pKa = 10.62KK38 pKa = 9.15KK39 pKa = 9.62NKK41 pKa = 8.94KK42 pKa = 8.52KK43 pKa = 10.23NYY45 pKa = 9.86NIFFFLLSRR54 pKa = 4.14

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

15674

32

1204

186.6

20.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.039 ± 0.404

1.429 ± 0.165

6.087 ± 0.206

6.342 ± 0.345

3.968 ± 0.189

7.337 ± 0.432

2.233 ± 0.152

6.15 ± 0.268

6.399 ± 0.392

7.56 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.845 ± 0.183

4.887 ± 0.183

3.739 ± 0.141

4.115 ± 0.185

4.619 ± 0.232

6.622 ± 0.322

5.697 ± 0.294

6.935 ± 0.3

1.467 ± 0.098

3.528 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski