Amino acid dipepetide frequency for Escherichia phage vB_EcoM_4HA13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.593AlaAla: 7.593 ± 1.005
1.085AlaCys: 1.085 ± 0.272
4.53AlaAsp: 4.53 ± 0.734
4.339AlaGlu: 4.339 ± 0.515
2.999AlaPhe: 2.999 ± 0.455
6.508AlaGly: 6.508 ± 1.087
1.978AlaHis: 1.978 ± 0.324
4.083AlaIle: 4.083 ± 0.498
5.36AlaLys: 5.36 ± 0.631
6.508AlaLeu: 6.508 ± 0.726
2.552AlaMet: 2.552 ± 0.473
4.147AlaAsn: 4.147 ± 0.487
2.488AlaPro: 2.488 ± 0.581
3.956AlaGln: 3.956 ± 0.511
4.147AlaArg: 4.147 ± 0.848
4.466AlaSer: 4.466 ± 0.627
5.104AlaThr: 5.104 ± 0.686
5.232AlaVal: 5.232 ± 0.565
0.638AlaTrp: 0.638 ± 0.193
2.68AlaTyr: 2.68 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
0.638CysAla: 0.638 ± 0.221
0.255CysCys: 0.255 ± 0.106
1.276CysAsp: 1.276 ± 0.282
0.829CysGlu: 0.829 ± 0.224
0.766CysPhe: 0.766 ± 0.184
0.957CysGly: 0.957 ± 0.27
0.447CysHis: 0.447 ± 0.211
0.957CysIle: 0.957 ± 0.249
1.021CysLys: 1.021 ± 0.295
0.893CysLeu: 0.893 ± 0.25
0.191CysMet: 0.191 ± 0.119
0.702CysAsn: 0.702 ± 0.242
0.319CysPro: 0.319 ± 0.133
0.447CysGln: 0.447 ± 0.162
0.766CysArg: 0.766 ± 0.234
1.021CysSer: 1.021 ± 0.304
1.085CysThr: 1.085 ± 0.255
0.957CysVal: 0.957 ± 0.274
0.255CysTrp: 0.255 ± 0.118
0.51CysTyr: 0.51 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
5.296AspAla: 5.296 ± 0.489
1.085AspCys: 1.085 ± 0.275
3.892AspAsp: 3.892 ± 0.618
4.083AspGlu: 4.083 ± 0.573
3.126AspPhe: 3.126 ± 0.424
5.232AspGly: 5.232 ± 0.632
0.893AspHis: 0.893 ± 0.247
3.509AspIle: 3.509 ± 0.524
3.956AspLys: 3.956 ± 0.504
4.721AspLeu: 4.721 ± 0.562
1.404AspMet: 1.404 ± 0.223
2.999AspAsn: 2.999 ± 0.371
2.297AspPro: 2.297 ± 0.509
1.085AspGln: 1.085 ± 0.226
2.042AspArg: 2.042 ± 0.37
4.02AspSer: 4.02 ± 0.599
3.254AspThr: 3.254 ± 0.426
4.466AspVal: 4.466 ± 0.553
1.148AspTrp: 1.148 ± 0.247
2.361AspTyr: 2.361 ± 0.363
0.0AspXaa: 0.0 ± 0.0
Glu
6.061GluAla: 6.061 ± 0.642
0.702GluCys: 0.702 ± 0.231
3.509GluAsp: 3.509 ± 0.462
3.764GluGlu: 3.764 ± 0.61
2.169GluPhe: 2.169 ± 0.334
3.892GluGly: 3.892 ± 0.403
2.106GluHis: 2.106 ± 0.313
5.168GluIle: 5.168 ± 0.736
2.744GluLys: 2.744 ± 0.45
6.189GluLeu: 6.189 ± 0.535
1.659GluMet: 1.659 ± 0.309
2.488GluAsn: 2.488 ± 0.463
1.723GluPro: 1.723 ± 0.338
2.297GluGln: 2.297 ± 0.349
3.509GluArg: 3.509 ± 0.559
3.892GluSer: 3.892 ± 0.423
3.254GluThr: 3.254 ± 0.492
4.53GluVal: 4.53 ± 0.604
1.34GluTrp: 1.34 ± 0.271
2.425GluTyr: 2.425 ± 0.438
0.0GluXaa: 0.0 ± 0.0
Phe
2.807PheAla: 2.807 ± 0.549
0.51PheCys: 0.51 ± 0.186
3.828PheAsp: 3.828 ± 0.426
2.807PheGlu: 2.807 ± 0.445
1.021PhePhe: 1.021 ± 0.254
3.509PheGly: 3.509 ± 0.495
1.276PheHis: 1.276 ± 0.289
2.871PheIle: 2.871 ± 0.341
2.744PheLys: 2.744 ± 0.419
2.999PheLeu: 2.999 ± 0.46
1.404PheMet: 1.404 ± 0.257
1.659PheAsn: 1.659 ± 0.494
1.085PhePro: 1.085 ± 0.274
1.467PheGln: 1.467 ± 0.347
1.531PheArg: 1.531 ± 0.304
2.106PheSer: 2.106 ± 0.256
2.425PheThr: 2.425 ± 0.412
1.787PheVal: 1.787 ± 0.342
0.51PheTrp: 0.51 ± 0.164
1.34PheTyr: 1.34 ± 0.346
0.0PheXaa: 0.0 ± 0.0
Gly
5.742GlyAla: 5.742 ± 1.025
0.702GlyCys: 0.702 ± 0.193
4.849GlyAsp: 4.849 ± 0.544
4.275GlyGlu: 4.275 ± 0.461
3.445GlyPhe: 3.445 ± 0.433
6.125GlyGly: 6.125 ± 0.987
1.914GlyHis: 1.914 ± 0.41
4.53GlyIle: 4.53 ± 0.613
4.658GlyLys: 4.658 ± 0.718
4.594GlyLeu: 4.594 ± 0.527
2.297GlyMet: 2.297 ± 0.478
2.999GlyAsn: 2.999 ± 0.43
2.106GlyPro: 2.106 ± 0.284
2.233GlyGln: 2.233 ± 0.293
3.254GlyArg: 3.254 ± 0.617
4.02GlySer: 4.02 ± 0.562
5.423GlyThr: 5.423 ± 0.849
6.189GlyVal: 6.189 ± 0.633
1.595GlyTrp: 1.595 ± 0.281
2.425GlyTyr: 2.425 ± 0.357
0.0GlyXaa: 0.0 ± 0.0
His
1.404HisAla: 1.404 ± 0.293
0.51HisCys: 0.51 ± 0.172
1.978HisAsp: 1.978 ± 0.394
1.276HisGlu: 1.276 ± 0.294
0.957HisPhe: 0.957 ± 0.285
1.85HisGly: 1.85 ± 0.331
0.638HisHis: 0.638 ± 0.203
0.957HisIle: 0.957 ± 0.232
1.595HisLys: 1.595 ± 0.287
1.467HisLeu: 1.467 ± 0.254
0.829HisMet: 0.829 ± 0.244
1.404HisAsn: 1.404 ± 0.277
1.148HisPro: 1.148 ± 0.25
0.829HisGln: 0.829 ± 0.202
0.702HisArg: 0.702 ± 0.22
1.276HisSer: 1.276 ± 0.273
0.638HisThr: 0.638 ± 0.211
1.404HisVal: 1.404 ± 0.289
0.447HisTrp: 0.447 ± 0.173
1.021HisTyr: 1.021 ± 0.234
0.0HisXaa: 0.0 ± 0.0
Ile
4.594IleAla: 4.594 ± 0.477
1.148IleCys: 1.148 ± 0.242
4.083IleAsp: 4.083 ± 0.578
4.083IleGlu: 4.083 ± 0.445
1.978IlePhe: 1.978 ± 0.343
4.147IleGly: 4.147 ± 0.529
0.702IleHis: 0.702 ± 0.196
3.892IleIle: 3.892 ± 0.559
4.977IleLys: 4.977 ± 0.718
3.637IleLeu: 3.637 ± 0.549
1.723IleMet: 1.723 ± 0.384
3.063IleAsn: 3.063 ± 0.459
3.254IlePro: 3.254 ± 0.446
2.68IleGln: 2.68 ± 0.349
3.318IleArg: 3.318 ± 0.42
3.892IleSer: 3.892 ± 0.651
3.828IleThr: 3.828 ± 0.379
3.828IleVal: 3.828 ± 0.53
1.212IleTrp: 1.212 ± 0.24
1.467IleTyr: 1.467 ± 0.301
0.0IleXaa: 0.0 ± 0.0
Lys
4.594LysAla: 4.594 ± 0.497
1.467LysCys: 1.467 ± 0.424
2.999LysAsp: 2.999 ± 0.452
4.275LysGlu: 4.275 ± 0.612
2.297LysPhe: 2.297 ± 0.393
3.828LysGly: 3.828 ± 0.52
1.978LysHis: 1.978 ± 0.385
3.573LysIle: 3.573 ± 0.496
3.637LysLys: 3.637 ± 0.601
6.317LysLeu: 6.317 ± 0.814
2.488LysMet: 2.488 ± 0.404
2.871LysAsn: 2.871 ± 0.432
2.68LysPro: 2.68 ± 0.505
3.509LysGln: 3.509 ± 0.514
2.616LysArg: 2.616 ± 0.407
4.211LysSer: 4.211 ± 0.474
3.382LysThr: 3.382 ± 0.523
4.339LysVal: 4.339 ± 0.716
0.574LysTrp: 0.574 ± 0.19
2.361LysTyr: 2.361 ± 0.439
0.0LysXaa: 0.0 ± 0.0
Leu
5.806LeuAla: 5.806 ± 0.61
0.957LeuCys: 0.957 ± 0.263
3.956LeuAsp: 3.956 ± 0.569
6.061LeuGlu: 6.061 ± 0.755
2.68LeuPhe: 2.68 ± 0.405
4.913LeuGly: 4.913 ± 0.538
1.404LeuHis: 1.404 ± 0.335
4.402LeuIle: 4.402 ± 0.459
5.041LeuLys: 5.041 ± 0.609
5.806LeuLeu: 5.806 ± 0.707
1.723LeuMet: 1.723 ± 0.273
4.658LeuAsn: 4.658 ± 0.534
3.382LeuPro: 3.382 ± 0.446
2.807LeuGln: 2.807 ± 0.419
3.637LeuArg: 3.637 ± 0.554
5.104LeuSer: 5.104 ± 0.588
4.466LeuThr: 4.466 ± 0.453
5.934LeuVal: 5.934 ± 0.702
0.766LeuTrp: 0.766 ± 0.247
2.106LeuTyr: 2.106 ± 0.329
0.0LeuXaa: 0.0 ± 0.0
Met
3.19MetAla: 3.19 ± 0.448
0.383MetCys: 0.383 ± 0.15
1.34MetAsp: 1.34 ± 0.344
1.531MetGlu: 1.531 ± 0.258
0.893MetPhe: 0.893 ± 0.22
1.404MetGly: 1.404 ± 0.443
0.51MetHis: 0.51 ± 0.151
1.148MetIle: 1.148 ± 0.284
2.361MetLys: 2.361 ± 0.352
2.297MetLeu: 2.297 ± 0.417
0.829MetMet: 0.829 ± 0.195
1.34MetAsn: 1.34 ± 0.301
1.34MetPro: 1.34 ± 0.311
1.85MetGln: 1.85 ± 0.347
1.021MetArg: 1.021 ± 0.325
2.361MetSer: 2.361 ± 0.467
1.34MetThr: 1.34 ± 0.269
1.659MetVal: 1.659 ± 0.301
0.447MetTrp: 0.447 ± 0.186
1.212MetTyr: 1.212 ± 0.261
0.0MetXaa: 0.0 ± 0.0
Asn
4.275AsnAla: 4.275 ± 0.539
0.702AsnCys: 0.702 ± 0.23
2.552AsnAsp: 2.552 ± 0.449
1.85AsnGlu: 1.85 ± 0.329
1.914AsnPhe: 1.914 ± 0.38
3.956AsnGly: 3.956 ± 0.595
0.766AsnHis: 0.766 ± 0.26
2.744AsnIle: 2.744 ± 0.403
3.509AsnLys: 3.509 ± 0.608
4.083AsnLeu: 4.083 ± 0.436
1.404AsnMet: 1.404 ± 0.329
2.361AsnAsn: 2.361 ± 0.431
3.063AsnPro: 3.063 ± 0.399
1.978AsnGln: 1.978 ± 0.402
2.297AsnArg: 2.297 ± 0.372
3.509AsnSer: 3.509 ± 0.49
2.552AsnThr: 2.552 ± 0.408
2.935AsnVal: 2.935 ± 0.474
0.766AsnTrp: 0.766 ± 0.217
1.659AsnTyr: 1.659 ± 0.365
0.0AsnXaa: 0.0 ± 0.0
Pro
3.573ProAla: 3.573 ± 0.449
0.447ProCys: 0.447 ± 0.153
2.297ProAsp: 2.297 ± 0.315
3.19ProGlu: 3.19 ± 0.436
1.531ProPhe: 1.531 ± 0.259
2.488ProGly: 2.488 ± 0.375
1.085ProHis: 1.085 ± 0.232
2.042ProIle: 2.042 ± 0.391
2.169ProLys: 2.169 ± 0.396
1.914ProLeu: 1.914 ± 0.329
0.893ProMet: 0.893 ± 0.229
2.169ProAsn: 2.169 ± 0.312
1.212ProPro: 1.212 ± 0.289
1.595ProGln: 1.595 ± 0.362
1.659ProArg: 1.659 ± 0.306
2.68ProSer: 2.68 ± 0.438
1.914ProThr: 1.914 ± 0.371
2.744ProVal: 2.744 ± 0.354
0.574ProTrp: 0.574 ± 0.161
1.212ProTyr: 1.212 ± 0.368
0.0ProXaa: 0.0 ± 0.0
Gln
2.935GlnAla: 2.935 ± 0.507
0.51GlnCys: 0.51 ± 0.164
1.978GlnAsp: 1.978 ± 0.347
2.935GlnGlu: 2.935 ± 0.336
1.723GlnPhe: 1.723 ± 0.314
2.169GlnGly: 2.169 ± 0.416
0.638GlnHis: 0.638 ± 0.188
2.488GlnIle: 2.488 ± 0.348
2.042GlnLys: 2.042 ± 0.383
3.445GlnLeu: 3.445 ± 0.494
1.212GlnMet: 1.212 ± 0.242
1.723GlnAsn: 1.723 ± 0.291
1.276GlnPro: 1.276 ± 0.307
2.616GlnGln: 2.616 ± 0.562
2.233GlnArg: 2.233 ± 0.407
2.871GlnSer: 2.871 ± 0.5
2.68GlnThr: 2.68 ± 0.407
3.19GlnVal: 3.19 ± 0.404
0.383GlnTrp: 0.383 ± 0.15
2.106GlnTyr: 2.106 ± 0.336
0.0GlnXaa: 0.0 ± 0.0
Arg
3.701ArgAla: 3.701 ± 0.713
0.957ArgCys: 0.957 ± 0.305
2.616ArgAsp: 2.616 ± 0.493
2.999ArgGlu: 2.999 ± 0.41
2.042ArgPhe: 2.042 ± 0.471
2.488ArgGly: 2.488 ± 0.372
0.957ArgHis: 0.957 ± 0.246
3.254ArgIle: 3.254 ± 0.381
2.68ArgLys: 2.68 ± 0.376
3.701ArgLeu: 3.701 ± 0.479
1.34ArgMet: 1.34 ± 0.344
2.297ArgAsn: 2.297 ± 0.345
1.659ArgPro: 1.659 ± 0.31
1.723ArgGln: 1.723 ± 0.329
2.106ArgArg: 2.106 ± 0.41
3.126ArgSer: 3.126 ± 0.431
2.488ArgThr: 2.488 ± 0.448
3.509ArgVal: 3.509 ± 0.484
0.766ArgTrp: 0.766 ± 0.26
1.723ArgTyr: 1.723 ± 0.303
0.0ArgXaa: 0.0 ± 0.0
Ser
5.104SerAla: 5.104 ± 0.946
0.574SerCys: 0.574 ± 0.215
3.956SerAsp: 3.956 ± 0.453
4.275SerGlu: 4.275 ± 0.525
2.807SerPhe: 2.807 ± 0.46
6.317SerGly: 6.317 ± 0.797
1.85SerHis: 1.85 ± 0.286
4.658SerIle: 4.658 ± 0.611
3.956SerLys: 3.956 ± 0.451
4.785SerLeu: 4.785 ± 0.36
1.659SerMet: 1.659 ± 0.26
2.999SerAsn: 2.999 ± 0.448
1.659SerPro: 1.659 ± 0.353
3.254SerGln: 3.254 ± 0.559
3.573SerArg: 3.573 ± 0.488
4.147SerSer: 4.147 ± 0.601
2.807SerThr: 2.807 ± 0.401
4.721SerVal: 4.721 ± 0.603
1.085SerTrp: 1.085 ± 0.258
2.042SerTyr: 2.042 ± 0.291
0.0SerXaa: 0.0 ± 0.0
Thr
4.339ThrAla: 4.339 ± 0.623
0.447ThrCys: 0.447 ± 0.187
3.573ThrAsp: 3.573 ± 0.587
3.892ThrGlu: 3.892 ± 0.417
2.297ThrPhe: 2.297 ± 0.397
5.487ThrGly: 5.487 ± 0.571
0.638ThrHis: 0.638 ± 0.193
3.764ThrIle: 3.764 ± 0.473
3.19ThrLys: 3.19 ± 0.426
4.785ThrLeu: 4.785 ± 0.597
1.148ThrMet: 1.148 ± 0.249
2.361ThrAsn: 2.361 ± 0.436
2.297ThrPro: 2.297 ± 0.33
1.787ThrGln: 1.787 ± 0.322
2.106ThrArg: 2.106 ± 0.321
4.147ThrSer: 4.147 ± 0.517
3.063ThrThr: 3.063 ± 0.463
4.466ThrVal: 4.466 ± 0.637
0.766ThrTrp: 0.766 ± 0.21
2.425ThrTyr: 2.425 ± 0.356
0.0ThrXaa: 0.0 ± 0.0
Val
5.551ValAla: 5.551 ± 0.684
0.957ValCys: 0.957 ± 0.309
4.849ValAsp: 4.849 ± 0.594
4.083ValGlu: 4.083 ± 0.608
3.063ValPhe: 3.063 ± 0.603
4.402ValGly: 4.402 ± 0.441
1.595ValHis: 1.595 ± 0.344
4.466ValIle: 4.466 ± 0.625
4.275ValLys: 4.275 ± 0.441
4.402ValLeu: 4.402 ± 0.601
2.106ValMet: 2.106 ± 0.427
4.02ValAsn: 4.02 ± 0.537
2.169ValPro: 2.169 ± 0.347
3.126ValGln: 3.126 ± 0.42
3.382ValArg: 3.382 ± 0.46
4.53ValSer: 4.53 ± 0.601
4.594ValThr: 4.594 ± 0.63
4.466ValVal: 4.466 ± 0.615
0.829ValTrp: 0.829 ± 0.18
2.488ValTyr: 2.488 ± 0.424
0.0ValXaa: 0.0 ± 0.0
Trp
0.893TrpAla: 0.893 ± 0.232
0.383TrpCys: 0.383 ± 0.171
0.766TrpAsp: 0.766 ± 0.259
1.212TrpGlu: 1.212 ± 0.331
0.574TrpPhe: 0.574 ± 0.183
0.574TrpGly: 0.574 ± 0.198
0.255TrpHis: 0.255 ± 0.126
0.829TrpIle: 0.829 ± 0.23
1.085TrpLys: 1.085 ± 0.238
0.957TrpLeu: 0.957 ± 0.269
0.319TrpMet: 0.319 ± 0.159
1.021TrpAsn: 1.021 ± 0.236
0.51TrpPro: 0.51 ± 0.157
0.638TrpGln: 0.638 ± 0.155
0.766TrpArg: 0.766 ± 0.21
1.212TrpSer: 1.212 ± 0.286
1.212TrpThr: 1.212 ± 0.266
0.51TrpVal: 0.51 ± 0.151
0.191TrpTrp: 0.191 ± 0.11
0.766TrpTyr: 0.766 ± 0.225
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.297TyrAla: 2.297 ± 0.37
0.51TyrCys: 0.51 ± 0.184
2.042TyrAsp: 2.042 ± 0.365
1.659TyrGlu: 1.659 ± 0.361
1.404TyrPhe: 1.404 ± 0.324
3.126TyrGly: 3.126 ± 0.483
0.702TyrHis: 0.702 ± 0.272
2.169TyrIle: 2.169 ± 0.347
2.999TyrLys: 2.999 ± 0.514
2.106TyrLeu: 2.106 ± 0.417
1.212TyrMet: 1.212 ± 0.325
1.595TyrAsn: 1.595 ± 0.239
1.723TyrPro: 1.723 ± 0.311
1.276TyrGln: 1.276 ± 0.219
1.531TyrArg: 1.531 ± 0.315
3.637TyrSer: 3.637 ± 0.514
1.467TyrThr: 1.467 ± 0.321
2.488TyrVal: 2.488 ± 0.489
0.383TyrTrp: 0.383 ± 0.167
0.957TyrTyr: 0.957 ± 0.233
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (15674 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski