Aspergillus calidoustus
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15359 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U5G0G1|A0A0U5G0G1_9EURO Uncharacterized protein OS=Aspergillus calidoustus OX=454130 GN=ASPCAL06346 PE=3 SV=1
MM1 pKa = 7.62 HH2 pKa = 7.55 SLTINLTLALFSLLTPTIHH21 pKa = 6.39 AQNFTTFHH29 pKa = 7.68 DD30 pKa = 4.18 YY31 pKa = 11.27 LSTYY35 pKa = 9.89 IPNCILDD42 pKa = 3.99 CTISAAEE49 pKa = 4.38 RR50 pKa = 11.84 DD51 pKa = 4.28 TEE53 pKa = 4.28 CGSGSVSSTDD63 pKa = 4.73 ADD65 pKa = 6.15 DD66 pKa = 5.06 IDD68 pKa = 4.52 CLCDD72 pKa = 4.73 AFWHH76 pKa = 5.78 TNGRR80 pKa = 11.84 FVRR83 pKa = 11.84 HH84 pKa = 5.99 FSEE87 pKa = 4.48 SMARR91 pKa = 11.84 CVTDD95 pKa = 3.65 ARR97 pKa = 11.84 CSLDD101 pKa = 3.23 EE102 pKa = 4.86 LEE104 pKa = 5.17 DD105 pKa = 3.72 VARR108 pKa = 11.84 VDD110 pKa = 3.07 PWTLFDD116 pKa = 5.89 GVDD119 pKa = 3.74 GLCGGRR125 pKa = 11.84 HH126 pKa = 5.03 GHH128 pKa = 6.7 DD129 pKa = 4.26 HH130 pKa = 7.41 DD131 pKa = 6.45 HH132 pKa = 7.86 DD133 pKa = 5.52 DD134 pKa = 5.55 DD135 pKa = 5.25 SNNSNDD141 pKa = 4.91 DD142 pKa = 3.33 EE143 pKa = 4.7 TSNSNSNNSNSSSNSDD159 pKa = 3.49 DD160 pKa = 3.99 TQDD163 pKa = 4.33 SSSDD167 pKa = 3.59 TANDD171 pKa = 3.56 TSDD174 pKa = 3.38 SSTSSSSNSGDD185 pKa = 3.78 DD186 pKa = 3.86 DD187 pKa = 4.06 NGAASIASRR196 pKa = 11.84 SITALAAGALVVLAAFF212 pKa = 4.8
Molecular weight: 22.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.859
IPC_protein 3.897
Toseland 3.668
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.897
Rodwell 3.719
Grimsley 3.567
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.355
Thurlkill 3.732
EMBOSS 3.897
Sillero 4.037
Patrickios 1.24
IPC_peptide 3.91
IPC2_peptide 3.999
IPC2.peptide.svr19 3.888
Protein with the highest isoelectric point:
>tr|A0A0U5CLP4|A0A0U5CLP4_9EURO Uncharacterized protein OS=Aspergillus calidoustus OX=454130 GN=ASPCAL01859 PE=4 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.84 LAKK15 pKa = 9.55 AQKK18 pKa = 8.59 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.41 TRR48 pKa = 11.84 LGII51 pKa = 4.46
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15115
244
15359
7021788
51
7770
457.2
50.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.645 ± 0.016
1.321 ± 0.008
5.579 ± 0.012
6.105 ± 0.018
3.772 ± 0.013
6.901 ± 0.018
2.456 ± 0.009
5.025 ± 0.013
4.298 ± 0.017
9.322 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.067 ± 0.008
3.529 ± 0.009
6.025 ± 0.019
3.941 ± 0.015
6.253 ± 0.017
8.114 ± 0.024
6.037 ± 0.014
6.208 ± 0.013
1.536 ± 0.008
2.864 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here