Amino acid dipepetide frequency for Aspergillus calidoustus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.07AlaAla: 9.07 ± 0.058	1.185AlaCys: 1.185 ± 0.015	4.276AlaAsp: 4.276 ± 0.026	5.147AlaGlu: 5.147 ± 0.03	3.224AlaPhe: 3.224 ± 0.025	5.932AlaGly: 5.932 ± 0.033	1.851AlaHis: 1.851 ± 0.015	4.481AlaIle: 4.481 ± 0.028	3.623AlaLys: 3.623 ± 0.026	8.314AlaLeu: 8.314 ± 0.043	1.932AlaMet: 1.932 ± 0.02	2.84AlaAsn: 2.84 ± 0.022	4.57AlaPro: 4.57 ± 0.041	3.389AlaGln: 3.389 ± 0.028	5.181AlaArg: 5.181 ± 0.031	7.028AlaSer: 7.028 ± 0.037	5.314AlaThr: 5.314 ± 0.028	5.596AlaVal: 5.596 ± 0.03	1.236AlaTrp: 1.236 ± 0.015	2.267AlaTyr: 2.267 ± 0.019	0.0AlaXaa: 0.0 ± 0.0
Cys	1.06CysAla: 1.06 ± 0.015	0.275CysCys: 0.275 ± 0.008	0.73CysAsp: 0.73 ± 0.012	0.643CysGlu: 0.643 ± 0.01	0.58CysPhe: 0.58 ± 0.01	0.972CysGly: 0.972 ± 0.014	0.355CysHis: 0.355 ± 0.007	0.725CysIle: 0.725 ± 0.012	0.475CysLys: 0.475 ± 0.008	1.392CysLeu: 1.392 ± 0.016	0.286CysMet: 0.286 ± 0.007	0.436CysAsn: 0.436 ± 0.007	0.73CysPro: 0.73 ± 0.013	0.481CysGln: 0.481 ± 0.009	0.86CysArg: 0.86 ± 0.012	0.989CysSer: 0.989 ± 0.013	0.756CysThr: 0.756 ± 0.011	0.86CysVal: 0.86 ± 0.012	0.215CysTrp: 0.215 ± 0.006	0.39CysTyr: 0.39 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	4.632AspAla: 4.632 ± 0.03	0.669AspCys: 0.669 ± 0.009	3.802AspAsp: 3.802 ± 0.031	4.229AspGlu: 4.229 ± 0.028	2.151AspPhe: 2.151 ± 0.018	3.989AspGly: 3.989 ± 0.025	1.263AspHis: 1.263 ± 0.014	3.152AspIle: 3.152 ± 0.025	2.131AspLys: 2.131 ± 0.021	5.178AspLeu: 5.178 ± 0.026	1.162AspMet: 1.162 ± 0.013	1.831AspAsn: 1.831 ± 0.017	3.396AspPro: 3.396 ± 0.026	1.863AspGln: 1.863 ± 0.018	3.106AspArg: 3.106 ± 0.027	4.062AspSer: 4.062 ± 0.025	3.046AspThr: 3.046 ± 0.024	3.568AspVal: 3.568 ± 0.022	0.92AspTrp: 0.92 ± 0.011	1.638AspTyr: 1.638 ± 0.017	0.0AspXaa: 0.0 ± 0.0
Glu	5.251GluAla: 5.251 ± 0.035	0.692GluCys: 0.692 ± 0.011	3.926GluAsp: 3.926 ± 0.029	5.132GluGlu: 5.132 ± 0.045	2.001GluPhe: 2.001 ± 0.017	3.827GluGly: 3.827 ± 0.028	1.407GluHis: 1.407 ± 0.015	3.163GluIle: 3.163 ± 0.023	3.316GluLys: 3.316 ± 0.025	5.2GluLeu: 5.2 ± 0.032	1.392GluMet: 1.392 ± 0.015	2.244GluAsn: 2.244 ± 0.018	2.865GluPro: 2.865 ± 0.03	2.455GluGln: 2.455 ± 0.023	3.998GluArg: 3.998 ± 0.059	4.326GluSer: 4.326 ± 0.03	3.669GluThr: 3.669 ± 0.024	3.548GluVal: 3.548 ± 0.023	0.892GluTrp: 0.892 ± 0.012	1.744GluTyr: 1.744 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	3.154PheAla: 3.154 ± 0.025	0.589PheCys: 0.589 ± 0.009	2.275PheAsp: 2.275 ± 0.018	2.142PheGlu: 2.142 ± 0.019	1.714PhePhe: 1.714 ± 0.02	2.891PheGly: 2.891 ± 0.025	0.933PheHis: 0.933 ± 0.011	1.871PheIle: 1.871 ± 0.019	1.352PheLys: 1.352 ± 0.015	3.68PheLeu: 3.68 ± 0.026	0.756PheMet: 0.756 ± 0.011	1.372PheAsn: 1.372 ± 0.015	2.066PhePro: 2.066 ± 0.018	1.397PheGln: 1.397 ± 0.016	2.089PheArg: 2.089 ± 0.019	2.992PheSer: 2.992 ± 0.023	2.187PheThr: 2.187 ± 0.02	2.414PheVal: 2.414 ± 0.021	0.696PheTrp: 0.696 ± 0.01	1.153PheTyr: 1.153 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	5.473GlyAla: 5.473 ± 0.034	0.945GlyCys: 0.945 ± 0.012	3.657GlyAsp: 3.657 ± 0.026	3.74GlyGlu: 3.74 ± 0.027	2.906GlyPhe: 2.906 ± 0.026	5.554GlyGly: 5.554 ± 0.043	1.747GlyHis: 1.747 ± 0.019	3.667GlyIle: 3.667 ± 0.03	3.182GlyLys: 3.182 ± 0.057	6.403GlyLeu: 6.403 ± 0.033	1.545GlyMet: 1.545 ± 0.016	2.469GlyAsn: 2.469 ± 0.02	3.356GlyPro: 3.356 ± 0.026	2.536GlyGln: 2.536 ± 0.02	4.152GlyArg: 4.152 ± 0.026	5.628GlySer: 5.628 ± 0.036	3.959GlyThr: 3.959 ± 0.024	4.624GlyVal: 4.624 ± 0.029	1.239GlyTrp: 1.239 ± 0.014	2.222GlyTyr: 2.222 ± 0.02	0.0GlyXaa: 0.0 ± 0.0
His	1.931HisAla: 1.931 ± 0.017	0.362HisCys: 0.362 ± 0.008	1.315HisAsp: 1.315 ± 0.012	1.365HisGlu: 1.365 ± 0.015	0.94HisPhe: 0.94 ± 0.012	1.801HisGly: 1.801 ± 0.02	0.847HisHis: 0.847 ± 0.014	1.268HisIle: 1.268 ± 0.011	0.846HisLys: 0.846 ± 0.011	2.418HisLeu: 2.418 ± 0.021	0.476HisMet: 0.476 ± 0.009	0.841HisAsn: 0.841 ± 0.011	1.723HisPro: 1.723 ± 0.015	0.968HisGln: 0.968 ± 0.012	1.617HisArg: 1.617 ± 0.017	1.881HisSer: 1.881 ± 0.018	1.372HisThr: 1.372 ± 0.014	1.457HisVal: 1.457 ± 0.014	0.396HisTrp: 0.396 ± 0.008	0.736HisTyr: 0.736 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	4.433IleAla: 4.433 ± 0.026	0.816IleCys: 0.816 ± 0.013	2.829IleAsp: 2.829 ± 0.022	2.873IleGlu: 2.873 ± 0.022	2.042IlePhe: 2.042 ± 0.021	3.247IleGly: 3.247 ± 0.026	1.284IleHis: 1.284 ± 0.014	2.594IleIle: 2.594 ± 0.023	1.929IleLys: 1.929 ± 0.016	4.907IleLeu: 4.907 ± 0.03	0.999IleMet: 0.999 ± 0.013	1.775IleAsn: 1.775 ± 0.016	3.233IlePro: 3.233 ± 0.024	1.983IleGln: 1.983 ± 0.018	2.974IleArg: 2.974 ± 0.025	3.939IleSer: 3.939 ± 0.022	2.927IleThr: 2.927 ± 0.021	3.202IleVal: 3.202 ± 0.024	0.775IleTrp: 0.775 ± 0.011	1.487IleTyr: 1.487 ± 0.015	0.001IleXaa: 0.001 ± 0.0
Lys	3.751LysAla: 3.751 ± 0.023	0.499LysCys: 0.499 ± 0.009	2.426LysAsp: 2.426 ± 0.02	3.026LysGlu: 3.026 ± 0.057	1.276LysPhe: 1.276 ± 0.013	2.762LysGly: 2.762 ± 0.02	1.049LysHis: 1.049 ± 0.011	2.038LysIle: 2.038 ± 0.018	2.641LysLys: 2.641 ± 0.035	3.76LysLeu: 3.76 ± 0.027	0.864LysMet: 0.864 ± 0.012	1.498LysAsn: 1.498 ± 0.015	2.475LysPro: 2.475 ± 0.024	1.663LysGln: 1.663 ± 0.017	3.158LysArg: 3.158 ± 0.028	3.078LysSer: 3.078 ± 0.026	2.53LysThr: 2.53 ± 0.021	2.557LysVal: 2.557 ± 0.022	0.642LysTrp: 0.642 ± 0.01	1.29LysTyr: 1.29 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	8.312LeuAla: 8.312 ± 0.038	1.287LeuCys: 1.287 ± 0.013	5.455LeuAsp: 5.455 ± 0.029	5.723LeuGlu: 5.723 ± 0.038	3.515LeuPhe: 3.515 ± 0.029	6.306LeuGly: 6.306 ± 0.039	2.445LeuHis: 2.445 ± 0.022	4.159LeuIle: 4.159 ± 0.028	3.883LeuLys: 3.883 ± 0.028	9.139LeuLeu: 9.139 ± 0.049	1.799LeuMet: 1.799 ± 0.015	3.208LeuAsn: 3.208 ± 0.02	5.684LeuPro: 5.684 ± 0.03	3.969LeuGln: 3.969 ± 0.03	6.047LeuArg: 6.047 ± 0.034	7.59LeuSer: 7.59 ± 0.031	5.013LeuThr: 5.013 ± 0.03	5.784LeuVal: 5.784 ± 0.035	1.309LeuTrp: 1.309 ± 0.015	2.586LeuTyr: 2.586 ± 0.02	0.0LeuXaa: 0.0 ± 0.0
Met	2.11MetAla: 2.11 ± 0.02	0.24MetCys: 0.24 ± 0.006	1.159MetAsp: 1.159 ± 0.014	1.218MetGlu: 1.218 ± 0.014	0.716MetPhe: 0.716 ± 0.011	1.417MetGly: 1.417 ± 0.014	0.486MetHis: 0.486 ± 0.008	0.992MetIle: 0.992 ± 0.012	0.88MetLys: 0.88 ± 0.012	1.829MetLeu: 1.829 ± 0.017	0.508MetMet: 0.508 ± 0.008	0.743MetAsn: 0.743 ± 0.012	1.22MetPro: 1.22 ± 0.057	0.812MetGln: 0.812 ± 0.01	1.227MetArg: 1.227 ± 0.015	1.742MetSer: 1.742 ± 0.016	1.257MetThr: 1.257 ± 0.013	1.322MetVal: 1.322 ± 0.015	0.267MetTrp: 0.267 ± 0.007	0.523MetTyr: 0.523 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	3.07AsnAla: 3.07 ± 0.023	0.469AsnCys: 0.469 ± 0.009	1.816AsnAsp: 1.816 ± 0.015	1.935AsnGlu: 1.935 ± 0.017	1.272AsnPhe: 1.272 ± 0.013	2.821AsnGly: 2.821 ± 0.022	0.856AsnHis: 0.856 ± 0.011	1.984AsnIle: 1.984 ± 0.017	1.348AsnLys: 1.348 ± 0.014	3.254AsnLeu: 3.254 ± 0.023	0.757AsnMet: 0.757 ± 0.01	1.374AsnAsn: 1.374 ± 0.017	2.484AsnPro: 2.484 ± 0.02	1.311AsnGln: 1.311 ± 0.016	1.988AsnArg: 1.988 ± 0.016	2.566AsnSer: 2.566 ± 0.023	2.147AsnThr: 2.147 ± 0.018	2.231AsnVal: 2.231 ± 0.018	0.565AsnTrp: 0.565 ± 0.01	1.045AsnTyr: 1.045 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	5.127ProAla: 5.127 ± 0.033	0.602ProCys: 0.602 ± 0.011	3.291ProAsp: 3.291 ± 0.023	3.875ProGlu: 3.875 ± 0.027	2.129ProPhe: 2.129 ± 0.018	4.033ProGly: 4.033 ± 0.03	1.367ProHis: 1.367 ± 0.016	2.603ProIle: 2.603 ± 0.02	2.335ProLys: 2.335 ± 0.024	5.013ProLeu: 5.013 ± 0.032	0.991ProMet: 0.991 ± 0.013	2.165ProAsn: 2.165 ± 0.018	4.767ProPro: 4.767 ± 0.057	2.464ProGln: 2.464 ± 0.057	3.495ProArg: 3.495 ± 0.026	5.969ProSer: 5.969 ± 0.048	4.013ProThr: 4.013 ± 0.028	3.658ProVal: 3.658 ± 0.028	0.823ProTrp: 0.823 ± 0.011	1.532ProTyr: 1.532 ± 0.017	0.0ProXaa: 0.0 ± 0.0
Gln	3.434GlnAla: 3.434 ± 0.027	0.483GlnCys: 0.483 ± 0.008	1.981GlnAsp: 1.981 ± 0.015	2.326GlnGlu: 2.326 ± 0.022	1.307GlnPhe: 1.307 ± 0.017	2.508GlnGly: 2.508 ± 0.059	1.074GlnHis: 1.074 ± 0.014	1.895GlnIle: 1.895 ± 0.016	1.765GlnLys: 1.765 ± 0.02	3.537GlnLeu: 3.537 ± 0.031	0.835GlnMet: 0.835 ± 0.012	1.477GlnAsn: 1.477 ± 0.017	2.482GlnPro: 2.482 ± 0.027	2.199GlnGln: 2.199 ± 0.036	2.688GlnArg: 2.688 ± 0.024	3.104GlnSer: 3.104 ± 0.027	2.364GlnThr: 2.364 ± 0.019	2.21GlnVal: 2.21 ± 0.02	0.596GlnTrp: 0.596 ± 0.01	1.152GlnTyr: 1.152 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	4.909ArgAla: 4.909 ± 0.029	0.801ArgCys: 0.801 ± 0.012	3.492ArgAsp: 3.492 ± 0.03	3.927ArgGlu: 3.927 ± 0.031	2.273ArgPhe: 2.273 ± 0.017	3.912ArgGly: 3.912 ± 0.029	1.631ArgHis: 1.631 ± 0.018	3.032ArgIle: 3.032 ± 0.02	3.209ArgLys: 3.209 ± 0.024	5.889ArgLeu: 5.889 ± 0.034	1.341ArgMet: 1.341 ± 0.053	2.246ArgAsn: 2.246 ± 0.018	3.474ArgPro: 3.474 ± 0.029	2.543ArgGln: 2.543 ± 0.023	5.151ArgArg: 5.151 ± 0.036	4.743ArgSer: 4.743 ± 0.033	3.491ArgThr: 3.491 ± 0.023	3.663ArgVal: 3.663 ± 0.02	1.008ArgTrp: 1.008 ± 0.012	1.793ArgTyr: 1.793 ± 0.016	0.0ArgXaa: 0.0 ± 0.0
Ser	6.613SerAla: 6.613 ± 0.04	0.963SerCys: 0.963 ± 0.014	4.152SerAsp: 4.152 ± 0.029	4.201SerGlu: 4.201 ± 0.029	3.049SerPhe: 3.049 ± 0.021	5.515SerGly: 5.515 ± 0.031	1.973SerHis: 1.973 ± 0.019	4.05SerIle: 4.05 ± 0.026	3.245SerLys: 3.245 ± 0.025	7.553SerLeu: 7.553 ± 0.038	1.605SerMet: 1.605 ± 0.016	2.801SerAsn: 2.801 ± 0.022	5.424SerPro: 5.424 ± 0.042	3.192SerGln: 3.192 ± 0.024	5.071SerArg: 5.071 ± 0.034	8.318SerSer: 8.318 ± 0.06	5.564SerThr: 5.564 ± 0.041	4.574SerVal: 4.574 ± 0.024	1.184SerTrp: 1.184 ± 0.013	2.097SerTyr: 2.097 ± 0.017	0.0SerXaa: 0.0 ± 0.0
Thr	5.308ThrAla: 5.308 ± 0.031	0.813ThrCys: 0.813 ± 0.012	2.915ThrAsp: 2.915 ± 0.02	3.214ThrGlu: 3.214 ± 0.025	2.289ThrPhe: 2.289 ± 0.021	4.295ThrGly: 4.295 ± 0.027	1.365ThrHis: 1.365 ± 0.014	3.167ThrIle: 3.167 ± 0.026	2.368ThrLys: 2.368 ± 0.019	5.574ThrLeu: 5.574 ± 0.03	1.15ThrMet: 1.15 ± 0.013	2.03ThrAsn: 2.03 ± 0.019	4.379ThrPro: 4.379 ± 0.033	2.1ThrGln: 2.1 ± 0.022	3.242ThrArg: 3.242 ± 0.023	5.256ThrSer: 5.256 ± 0.036	4.479ThrThr: 4.479 ± 0.045	3.874ThrVal: 3.874 ± 0.025	0.912ThrTrp: 0.912 ± 0.013	1.645ThrTyr: 1.645 ± 0.016	0.0ThrXaa: 0.0 ± 0.0
Val	5.299ValAla: 5.299 ± 0.031	0.886ValCys: 0.886 ± 0.011	3.742ValAsp: 3.742 ± 0.023	3.877ValGlu: 3.877 ± 0.025	2.531ValPhe: 2.531 ± 0.023	4.109ValGly: 4.109 ± 0.03	1.452ValHis: 1.452 ± 0.013	3.073ValIle: 3.073 ± 0.023	2.645ValLys: 2.645 ± 0.021	5.882ValLeu: 5.882 ± 0.03	1.253ValMet: 1.253 ± 0.016	2.172ValAsn: 2.172 ± 0.018	3.62ValPro: 3.62 ± 0.023	2.415ValGln: 2.415 ± 0.023	3.661ValArg: 3.661 ± 0.027	4.76ValSer: 4.76 ± 0.029	3.56ValThr: 3.56 ± 0.026	4.358ValVal: 4.358 ± 0.03	0.913ValTrp: 0.913 ± 0.013	1.876ValTyr: 1.876 ± 0.017	0.0ValXaa: 0.0 ± 0.0
Trp	1.207TrpAla: 1.207 ± 0.014	0.207TrpCys: 0.207 ± 0.005	0.93TrpAsp: 0.93 ± 0.013	0.91TrpGlu: 0.91 ± 0.012	0.575TrpPhe: 0.575 ± 0.009	0.975TrpGly: 0.975 ± 0.014	0.367TrpHis: 0.367 ± 0.009	0.823TrpIle: 0.823 ± 0.012	0.792TrpLys: 0.792 ± 0.009	1.473TrpLeu: 1.473 ± 0.017	0.377TrpMet: 0.377 ± 0.008	0.642TrpAsn: 0.642 ± 0.009	0.668TrpPro: 0.668 ± 0.01	0.566TrpGln: 0.566 ± 0.009	1.052TrpArg: 1.052 ± 0.014	1.098TrpSer: 1.098 ± 0.012	0.973TrpThr: 0.973 ± 0.014	0.962TrpVal: 0.962 ± 0.013	0.295TrpTrp: 0.295 ± 0.006	0.468TrpTyr: 0.468 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.31TyrAla: 2.31 ± 0.016	0.427TyrCys: 0.427 ± 0.008	1.62TyrAsp: 1.62 ± 0.016	1.547TyrGlu: 1.547 ± 0.017	1.233TyrPhe: 1.233 ± 0.015	2.138TyrGly: 2.138 ± 0.021	0.805TyrHis: 0.805 ± 0.011	1.51TyrIle: 1.51 ± 0.016	1.02TyrLys: 1.02 ± 0.013	2.819TyrLeu: 2.819 ± 0.022	0.641TyrMet: 0.641 ± 0.009	1.127TyrAsn: 1.127 ± 0.013	1.634TyrPro: 1.634 ± 0.019	1.11TyrGln: 1.11 ± 0.013	1.772TyrArg: 1.772 ± 0.016	2.075TyrSer: 2.075 ± 0.019	1.755TyrThr: 1.755 ± 0.017	1.62TyrVal: 1.62 ± 0.015	0.478TyrTrp: 0.478 ± 0.008	0.998TyrTyr: 0.998 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 15359 proteins (7021788 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---