Faecalibaculum rodentium
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2740 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140DXR4|A0A140DXR4_9FIRM Pyruvate formate-lyase OS=Faecalibaculum rodentium OX=1702221 GN=AALO17_23070 PE=4 SV=1
MM1 pKa = 7.81 ILDD4 pKa = 4.96 DD5 pKa = 6.1 LNFDD9 pKa = 5.98 DD10 pKa = 5.87 IDD12 pKa = 5.86 LDD14 pKa = 3.94 VLFGRR19 pKa = 11.84 DD20 pKa = 3.41 YY21 pKa = 11.33 DD22 pKa = 3.94 GKK24 pKa = 10.21 EE25 pKa = 3.84 FDD27 pKa = 3.19 MTEE30 pKa = 4.03 YY31 pKa = 10.55 VGVWNGYY38 pKa = 8.48 DD39 pKa = 3.32 VYY41 pKa = 11.12 LGYY44 pKa = 10.96 ADD46 pKa = 5.41 GEE48 pKa = 4.49 DD49 pKa = 3.26 HH50 pKa = 7.15 VLLVKK55 pKa = 10.32 GKK57 pKa = 9.85 RR58 pKa = 11.84 AWYY61 pKa = 7.87 PNSMDD66 pKa = 4.27 LDD68 pKa = 4.29 EE69 pKa = 4.97 IKK71 pKa = 11.1 YY72 pKa = 10.61 IFDD75 pKa = 3.75 LL76 pKa = 4.64
Molecular weight: 8.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.516
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.745
Rodwell 3.567
Grimsley 3.427
Solomon 3.745
Lehninger 3.706
Nozaki 3.884
DTASelect 4.177
Thurlkill 3.592
EMBOSS 3.745
Sillero 3.872
Patrickios 1.901
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>tr|A0A140DWL4|A0A140DWL4_9FIRM Uncharacterized protein OS=Faecalibaculum rodentium OX=1702221 GN=AALO17_19070 PE=3 SV=1
MM1 pKa = 7.1 KK2 pKa = 10.26 RR3 pKa = 11.84 KK4 pKa = 9.4 RR5 pKa = 11.84 QSDD8 pKa = 3.94 LLRR11 pKa = 11.84 QGNICQNQGNEE22 pKa = 3.96 GGKK25 pKa = 9.91 PMILVILGVIAVLSIRR41 pKa = 11.84 IALGLEE47 pKa = 4.03 NVRR50 pKa = 11.84 MKK52 pKa = 10.84 QLVHH56 pKa = 6.3 HH57 pKa = 7.02 RR58 pKa = 11.84 NAVWAQLDD66 pKa = 3.96 RR67 pKa = 11.84 QSLEE71 pKa = 4.03 PEE73 pKa = 3.81 QLEE76 pKa = 4.46 AARR79 pKa = 11.84 QQLRR83 pKa = 11.84 VSLLEE88 pKa = 3.94 NRR90 pKa = 11.84 HH91 pKa = 5.05 FRR93 pKa = 11.84 YY94 pKa = 10.06 ALPATRR100 pKa = 11.84 GFVLCALSAIACTLCLWMDD119 pKa = 3.79 ILTARR124 pKa = 11.84 NPAGFGFFHH133 pKa = 7.6 DD134 pKa = 4.73 LAAPTGVDD142 pKa = 3.06 ITCRR146 pKa = 11.84 ILLILYY152 pKa = 7.56 GLRR155 pKa = 11.84 LLVSVAVLPGRR166 pKa = 11.84 IRR168 pKa = 11.84 WVYY171 pKa = 10.96 LFTPLAAGLVLPFLYY186 pKa = 10.29 QCVSLSILSVWVTEE200 pKa = 4.02 QLWLLVSLMVLQHH213 pKa = 5.49 LTRR216 pKa = 11.84 LKK218 pKa = 10.61 RR219 pKa = 11.84 RR220 pKa = 11.84 GGGRR224 pKa = 11.84 KK225 pKa = 9.32 NSGG228 pKa = 2.9
Molecular weight: 25.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.589
IPC_protein 10.526
Toseland 10.613
ProMoST 10.394
Dawson 10.716
Bjellqvist 10.496
Wikipedia 10.965
Rodwell 10.818
Grimsley 10.774
Solomon 10.862
Lehninger 10.818
Nozaki 10.643
DTASelect 10.467
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.672
Patrickios 10.54
IPC_peptide 10.862
IPC2_peptide 9.867
IPC2.peptide.svr19 8.527
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2740
0
2740
719718
37
2967
262.7
29.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.515 ± 0.059
1.41 ± 0.021
6.168 ± 0.041
6.796 ± 0.047
3.961 ± 0.035
7.191 ± 0.048
1.968 ± 0.023
5.894 ± 0.04
5.256 ± 0.049
9.748 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.046 ± 0.028
3.475 ± 0.038
4.223 ± 0.034
3.994 ± 0.036
5.719 ± 0.057
5.837 ± 0.037
5.584 ± 0.047
6.757 ± 0.043
1.217 ± 0.018
3.24 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here