Desulfonatronum thiosulfatophilum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfonatronaceae; Desulfonatronum

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6AXB8|A0A1G6AXB8_9DELT Uncharacterized protein OS=Desulfonatronum thiosulfatophilum OX=617002 GN=SAMN05660653_00636 PE=4 SV=1
MM1 pKa = 7.53SYY3 pKa = 10.78KK4 pKa = 10.18VDD6 pKa = 4.36LNNMPPLIDD15 pKa = 4.03EE16 pKa = 4.32QNADD20 pKa = 3.87LKK22 pKa = 11.41ALFDD26 pKa = 4.22IPDD29 pKa = 3.86SEE31 pKa = 4.81IDD33 pKa = 3.78CSDD36 pKa = 3.7IPLLDD41 pKa = 4.48DD42 pKa = 4.27ALVNKK47 pKa = 7.61VACNPFYY54 pKa = 10.6NQPSPLPLIIQGAFSFVMLNN74 pKa = 3.2

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6CYL7|A0A1G6CYL7_9DELT NADH-quinone oxidoreductase subunit B OS=Desulfonatronum thiosulfatophilum OX=617002 GN=nuoB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.29QPSNTRR11 pKa = 11.84RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 6.56GFLVRR21 pKa = 11.84MKK23 pKa = 9.21TKK25 pKa = 10.22NGRR28 pKa = 11.84AIIRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3184

0

3184

1035802

39

2949

325.3

36.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.054 ± 0.043

1.194 ± 0.019

5.41 ± 0.033

6.489 ± 0.04

4.174 ± 0.033

7.489 ± 0.04

2.357 ± 0.023

5.667 ± 0.036

4.046 ± 0.043

11.221 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.02

3.163 ± 0.025

4.853 ± 0.031

3.978 ± 0.027

6.718 ± 0.036

5.644 ± 0.027

4.827 ± 0.022

7.106 ± 0.036

1.282 ± 0.017

2.501 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski