Fontimonas thermophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Nevskiales; Sinobacteraceae; Fontimonas

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2498 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2IJA3|A0A1I2IJA3_9GAMM Pyridoxine 5'-phosphate synthase OS=Fontimonas thermophila OX=1076937 GN=pdxJ PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 10.37SVNFSFAVGAVALAAATPAAFAQDD26 pKa = 3.84VAGSTASQVLAIPQGYY42 pKa = 8.71AASAFQVPIGSGAGWGGIGVGLYY65 pKa = 10.43GQTLDD70 pKa = 5.17DD71 pKa = 6.58DD72 pKa = 4.61IFADD76 pKa = 4.96DD77 pKa = 3.9YY78 pKa = 11.77DD79 pKa = 5.02GSVGLNIGLGDD90 pKa = 3.48PSKK93 pKa = 11.21YY94 pKa = 10.48VGLDD98 pKa = 2.97VSASFASLSDD108 pKa = 3.61ADD110 pKa = 4.53GSDD113 pKa = 3.97DD114 pKa = 3.76GLGEE118 pKa = 4.32AGSVGLKK125 pKa = 10.25LHH127 pKa = 6.57TNLPGYY133 pKa = 10.53ASVAIGVQTIGRR145 pKa = 11.84WGAVADD151 pKa = 4.26GGNEE155 pKa = 3.87SSVYY159 pKa = 10.62AVASKK164 pKa = 10.48YY165 pKa = 9.99FALGGQGLVATLGMGDD181 pKa = 3.76GAFHH185 pKa = 7.74DD186 pKa = 4.31EE187 pKa = 4.3ANGVGVFGALAYY199 pKa = 10.88YY200 pKa = 9.37PVTWFSVIGEE210 pKa = 4.22YY211 pKa = 9.54TGRR214 pKa = 11.84FANLAVSVAPIQRR227 pKa = 11.84WPVTLTAGAVNLGDD241 pKa = 4.22RR242 pKa = 11.84FDD244 pKa = 5.71LGTQFAASVGVGFSFF259 pKa = 4.82

Molecular weight: 25.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2H531|A0A1I2H531_9GAMM 3-deoxy-D-manno-octulosonic acid transferase OS=Fontimonas thermophila OX=1076937 GN=SAMN04488120_101141 PE=3 SV=1
MM1 pKa = 6.85ATKK4 pKa = 9.52RR5 pKa = 11.84TYY7 pKa = 9.71QPKK10 pKa = 9.1KK11 pKa = 9.24LRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 7.83RR16 pKa = 11.84THH18 pKa = 5.96GFRR21 pKa = 11.84ARR23 pKa = 11.84MATKK27 pKa = 10.23GGRR30 pKa = 11.84LVLSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.98GRR41 pKa = 11.84KK42 pKa = 8.89RR43 pKa = 11.84LIPP46 pKa = 4.02

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2498

0

2498

840331

40

2540

336.4

36.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.803 ± 0.067

1.029 ± 0.015

5.486 ± 0.041

5.549 ± 0.043

3.377 ± 0.027

8.158 ± 0.046

2.393 ± 0.025

4.791 ± 0.038

2.674 ± 0.049

10.992 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.06 ± 0.023

2.293 ± 0.028

5.564 ± 0.04

3.628 ± 0.028

8.29 ± 0.053

4.506 ± 0.03

5.029 ± 0.034

7.365 ± 0.04

1.471 ± 0.024

2.538 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski