Cassava virus C

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Miaviricetes; Ourlivirales; Botourmiaviridae; Ourmiavirus

Average proteome isoelectric point is 8.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5U1W8|B5U1W8_9VIRU Coat protein OS=Cassava virus C OX=561576 PE=2 SV=1
MM1 pKa = 7.78EE2 pKa = 5.48EE3 pKa = 5.04FPTIDD8 pKa = 3.51NRR10 pKa = 11.84IISADD15 pKa = 3.4LALPDD20 pKa = 4.27GNGNLAISIVEE31 pKa = 4.05PSSTLCLKK39 pKa = 10.76KK40 pKa = 10.18KK41 pKa = 10.13GHH43 pKa = 4.25VTYY46 pKa = 8.76RR47 pKa = 11.84TLMDD51 pKa = 4.79ASTSCQPQPLIPVSIWDD68 pKa = 3.76RR69 pKa = 11.84VRR71 pKa = 11.84LSAHH75 pKa = 5.49TAAHH79 pKa = 6.37RR80 pKa = 11.84NTYY83 pKa = 9.48VSYY86 pKa = 11.08EE87 pKa = 4.05EE88 pKa = 5.91VVAKK92 pKa = 8.95WVPSCLKK99 pKa = 10.74NEE101 pKa = 4.2AFGQFAVVDD110 pKa = 3.88TRR112 pKa = 11.84LQADD116 pKa = 4.14DD117 pKa = 4.9LEE119 pKa = 6.27DD120 pKa = 3.44MLRR123 pKa = 11.84RR124 pKa = 11.84AIWITDD130 pKa = 3.4EE131 pKa = 4.1VDD133 pKa = 3.12LGRR136 pKa = 11.84QYY138 pKa = 10.5TCSGALPFCLPVSSTDD154 pKa = 3.21RR155 pKa = 11.84GTINDD160 pKa = 3.67NPIKK164 pKa = 10.52VVLYY168 pKa = 6.54VTRR171 pKa = 11.84SGYY174 pKa = 9.93QEE176 pKa = 3.85AAKK179 pKa = 10.37LGSLTTTTKK188 pKa = 10.65LALSTSPIDD197 pKa = 4.31LVNTRR202 pKa = 11.84PAAFVEE208 pKa = 4.73KK209 pKa = 10.29KK210 pKa = 10.31AALKK214 pKa = 10.56IEE216 pKa = 4.13GTRR219 pKa = 11.84GGNLPSRR226 pKa = 11.84RR227 pKa = 11.84SSVEE231 pKa = 3.46TRR233 pKa = 11.84RR234 pKa = 11.84DD235 pKa = 3.26RR236 pKa = 11.84YY237 pKa = 10.36RR238 pKa = 11.84SGSARR243 pKa = 11.84WPARR247 pKa = 11.84DD248 pKa = 3.74RR249 pKa = 11.84DD250 pKa = 3.77EE251 pKa = 5.07SPTPGRR257 pKa = 11.84RR258 pKa = 11.84SFDD261 pKa = 3.44LPNRR265 pKa = 11.84LPVGGGTVPAAQDD278 pKa = 3.61PDD280 pKa = 4.2CFSSAGEE287 pKa = 4.11VGG289 pKa = 3.41

Molecular weight: 31.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5U1W8|B5U1W8_9VIRU Coat protein OS=Cassava virus C OX=561576 PE=2 SV=1
MM1 pKa = 7.11VARR4 pKa = 11.84SNRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84QNRR13 pKa = 11.84QPQGQITRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84NGGGNNNNPPRR34 pKa = 11.84SIPRR38 pKa = 11.84LAGTVIKK45 pKa = 10.41EE46 pKa = 4.46SIFEE50 pKa = 4.19SSFDD54 pKa = 3.97LDD56 pKa = 3.86LDD58 pKa = 3.76KK59 pKa = 11.34GANKK63 pKa = 10.47LKK65 pKa = 10.5VQALAFGPGATIGWTKK81 pKa = 10.1PPRR84 pKa = 11.84TIAWKK89 pKa = 10.31LKK91 pKa = 8.88TMQLDD96 pKa = 3.34WVSFLRR102 pKa = 11.84PTKK105 pKa = 10.34HH106 pKa = 6.54AGEE109 pKa = 4.32LKK111 pKa = 9.73ICLVPGRR118 pKa = 11.84GLNGLSKK125 pKa = 10.92DD126 pKa = 3.46QTLALAPAEE135 pKa = 4.13IARR138 pKa = 11.84SNSVRR143 pKa = 11.84TIRR146 pKa = 11.84VGGTLSRR153 pKa = 11.84NPWSGYY159 pKa = 6.71SQWVEE164 pKa = 3.78PPKK167 pKa = 10.69GVSNSGEE174 pKa = 4.28GEE176 pKa = 3.91NVAYY180 pKa = 9.83RR181 pKa = 11.84VVFDD185 pKa = 4.21PSNVLGTDD193 pKa = 3.04EE194 pKa = 4.59GEE196 pKa = 4.1KK197 pKa = 10.63LLRR200 pKa = 11.84VYY202 pKa = 10.44FKK204 pKa = 10.85LVYY207 pKa = 9.7QVQEE211 pKa = 3.95VAII214 pKa = 4.31

Molecular weight: 23.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1373

214

870

457.7

51.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.992 ± 0.577

1.748 ± 0.34

5.098 ± 0.689

5.39 ± 0.33

4.079 ± 0.806

7.065 ± 0.734

1.675 ± 0.475

5.171 ± 0.323

4.516 ± 0.428

9.905 ± 0.623

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.311 ± 0.169

3.714 ± 0.772

6.555 ± 0.133

3.132 ± 0.424

8.958 ± 0.127

8.012 ± 0.374

5.317 ± 0.952

6.919 ± 0.354

1.894 ± 0.186

2.549 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski