Amino acid dipepetide frequency for CrAssphage ZA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.125AlaAla: 4.125 ± 0.426
0.524AlaCys: 0.524 ± 0.156
2.75AlaAsp: 2.75 ± 0.325
3.994AlaGlu: 3.994 ± 0.434
2.455AlaPhe: 2.455 ± 0.275
3.83AlaGly: 3.83 ± 0.573
0.557AlaHis: 0.557 ± 0.104
5.402AlaIle: 5.402 ± 0.578
4.976AlaLys: 4.976 ± 0.485
3.765AlaLeu: 3.765 ± 0.373
1.113AlaMet: 1.113 ± 0.212
4.976AlaAsn: 4.976 ± 0.515
1.244AlaPro: 1.244 ± 0.199
2.292AlaGln: 2.292 ± 0.473
2.39AlaArg: 2.39 ± 0.313
4.027AlaSer: 4.027 ± 0.653
2.816AlaThr: 2.816 ± 0.522
3.536AlaVal: 3.536 ± 0.365
0.884AlaTrp: 0.884 ± 0.192
2.783AlaTyr: 2.783 ± 0.408
0.0AlaXaa: 0.0 ± 0.0
Cys
0.655CysAla: 0.655 ± 0.166
0.229CysCys: 0.229 ± 0.103
0.655CysAsp: 0.655 ± 0.171
0.589CysGlu: 0.589 ± 0.175
0.36CysPhe: 0.36 ± 0.129
0.72CysGly: 0.72 ± 0.173
0.164CysHis: 0.164 ± 0.073
0.884CysIle: 0.884 ± 0.173
0.851CysLys: 0.851 ± 0.201
0.72CysLeu: 0.72 ± 0.175
0.164CysMet: 0.164 ± 0.07
1.048CysAsn: 1.048 ± 0.224
0.262CysPro: 0.262 ± 0.114
0.426CysGln: 0.426 ± 0.161
0.458CysArg: 0.458 ± 0.126
0.295CysSer: 0.295 ± 0.104
0.262CysThr: 0.262 ± 0.106
0.884CysVal: 0.884 ± 0.206
0.229CysTrp: 0.229 ± 0.102
0.688CysTyr: 0.688 ± 0.236
0.0CysXaa: 0.0 ± 0.0
Asp
4.125AspAla: 4.125 ± 0.421
0.884AspCys: 0.884 ± 0.202
4.682AspAsp: 4.682 ± 0.403
4.256AspGlu: 4.256 ± 0.386
2.848AspPhe: 2.848 ± 0.279
5.107AspGly: 5.107 ± 0.364
0.393AspHis: 0.393 ± 0.124
7.235AspIle: 7.235 ± 0.493
6.941AspLys: 6.941 ± 0.534
3.503AspLeu: 3.503 ± 0.386
1.604AspMet: 1.604 ± 0.3
5.664AspAsn: 5.664 ± 0.434
0.818AspPro: 0.818 ± 0.171
0.753AspGln: 0.753 ± 0.174
2.455AspArg: 2.455 ± 0.258
4.06AspSer: 4.06 ± 0.382
4.125AspThr: 4.125 ± 0.316
3.11AspVal: 3.11 ± 0.346
1.048AspTrp: 1.048 ± 0.185
3.699AspTyr: 3.699 ± 0.288
0.0AspXaa: 0.0 ± 0.0
Glu
4.387GluAla: 4.387 ± 0.372
0.753GluCys: 0.753 ± 0.164
4.256GluAsp: 4.256 ± 0.408
5.042GluGlu: 5.042 ± 0.642
2.946GluPhe: 2.946 ± 0.39
2.357GluGly: 2.357 ± 0.345
1.244GluHis: 1.244 ± 0.224
4.387GluIle: 4.387 ± 0.374
3.503GluLys: 3.503 ± 0.368
6.089GluLeu: 6.089 ± 0.479
1.375GluMet: 1.375 ± 0.235
4.125GluAsn: 4.125 ± 0.415
1.473GluPro: 1.473 ± 0.272
2.979GluGln: 2.979 ± 0.4
2.881GluArg: 2.881 ± 0.438
3.143GluSer: 3.143 ± 0.351
3.012GluThr: 3.012 ± 0.351
3.765GluVal: 3.765 ± 0.336
0.753GluTrp: 0.753 ± 0.176
2.946GluTyr: 2.946 ± 0.234
0.0GluXaa: 0.0 ± 0.0
Phe
2.063PheAla: 2.063 ± 0.262
0.491PheCys: 0.491 ± 0.151
3.208PheAsp: 3.208 ± 0.36
2.783PheGlu: 2.783 ± 0.345
0.786PhePhe: 0.786 ± 0.145
2.324PheGly: 2.324 ± 0.296
0.36PheHis: 0.36 ± 0.124
3.503PheIle: 3.503 ± 0.407
3.601PheLys: 3.601 ± 0.383
1.964PheLeu: 1.964 ± 0.318
0.72PheMet: 0.72 ± 0.197
4.485PheAsn: 4.485 ± 0.452
1.08PhePro: 1.08 ± 0.209
0.655PheGln: 0.655 ± 0.142
1.44PheArg: 1.44 ± 0.267
1.997PheSer: 1.997 ± 0.237
2.161PheThr: 2.161 ± 0.337
2.39PheVal: 2.39 ± 0.344
0.426PheTrp: 0.426 ± 0.12
2.161PheTyr: 2.161 ± 0.265
0.0PheXaa: 0.0 ± 0.0
Gly
3.176GlyAla: 3.176 ± 0.484
0.491GlyCys: 0.491 ± 0.154
3.634GlyAsp: 3.634 ± 0.387
2.914GlyGlu: 2.914 ± 0.313
2.652GlyPhe: 2.652 ± 0.33
5.107GlyGly: 5.107 ± 0.9
0.655GlyHis: 0.655 ± 0.123
5.238GlyIle: 5.238 ± 0.499
4.551GlyLys: 4.551 ± 0.489
4.551GlyLeu: 4.551 ± 0.36
1.408GlyMet: 1.408 ± 0.266
5.271GlyAsn: 5.271 ± 0.41
0.426GlyPro: 0.426 ± 0.133
2.324GlyGln: 2.324 ± 0.305
2.554GlyArg: 2.554 ± 0.313
4.158GlySer: 4.158 ± 0.931
4.223GlyThr: 4.223 ± 0.63
3.503GlyVal: 3.503 ± 0.405
0.655GlyTrp: 0.655 ± 0.102
3.11GlyTyr: 3.11 ± 0.36
0.0GlyXaa: 0.0 ± 0.0
His
0.688HisAla: 0.688 ± 0.123
0.295HisCys: 0.295 ± 0.114
0.72HisAsp: 0.72 ± 0.139
0.884HisGlu: 0.884 ± 0.195
0.589HisPhe: 0.589 ± 0.129
0.884HisGly: 0.884 ± 0.172
0.164HisHis: 0.164 ± 0.078
1.506HisIle: 1.506 ± 0.274
1.015HisLys: 1.015 ± 0.189
0.949HisLeu: 0.949 ± 0.163
0.262HisMet: 0.262 ± 0.088
1.211HisAsn: 1.211 ± 0.189
0.72HisPro: 0.72 ± 0.15
0.295HisGln: 0.295 ± 0.091
0.688HisArg: 0.688 ± 0.146
0.688HisSer: 0.688 ± 0.146
0.72HisThr: 0.72 ± 0.184
0.753HisVal: 0.753 ± 0.195
0.131HisTrp: 0.131 ± 0.087
0.72HisTyr: 0.72 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
5.435IleAla: 5.435 ± 0.425
0.818IleCys: 0.818 ± 0.177
7.039IleAsp: 7.039 ± 0.443
4.813IleGlu: 4.813 ± 0.343
2.619IlePhe: 2.619 ± 0.494
5.14IleGly: 5.14 ± 0.398
1.211IleHis: 1.211 ± 0.159
7.792IleIle: 7.792 ± 0.614
7.563IleLys: 7.563 ± 0.669
4.747IleLeu: 4.747 ± 0.463
1.801IleMet: 1.801 ± 0.268
10.116IleAsn: 10.116 ± 0.705
2.979IlePro: 2.979 ± 0.331
2.652IleGln: 2.652 ± 0.285
3.208IleArg: 3.208 ± 0.386
5.467IleSer: 5.467 ± 0.418
6.548IleThr: 6.548 ± 0.588
4.289IleVal: 4.289 ± 0.46
0.491IleTrp: 0.491 ± 0.148
3.307IleTyr: 3.307 ± 0.34
0.0IleXaa: 0.0 ± 0.0
Lys
4.682LysAla: 4.682 ± 0.563
0.884LysCys: 0.884 ± 0.229
6.45LysAsp: 6.45 ± 0.472
6.613LysGlu: 6.613 ± 0.623
3.47LysPhe: 3.47 ± 0.304
4.485LysGly: 4.485 ± 0.34
1.244LysHis: 1.244 ± 0.259
4.682LysIle: 4.682 ± 0.415
4.354LysLys: 4.354 ± 0.506
8.054LysLeu: 8.054 ± 0.528
1.604LysMet: 1.604 ± 0.28
4.714LysAsn: 4.714 ± 0.436
1.768LysPro: 1.768 ± 0.237
2.881LysGln: 2.881 ± 0.358
3.11LysArg: 3.11 ± 0.445
3.765LysSer: 3.765 ± 0.348
4.42LysThr: 4.42 ± 0.369
5.271LysVal: 5.271 ± 0.535
0.589LysTrp: 0.589 ± 0.135
4.878LysTyr: 4.878 ± 0.405
0.0LysXaa: 0.0 ± 0.0
Leu
3.634LeuAla: 3.634 ± 0.385
0.786LeuCys: 0.786 ± 0.18
5.205LeuAsp: 5.205 ± 0.438
4.06LeuGlu: 4.06 ± 0.466
2.619LeuPhe: 2.619 ± 0.332
3.634LeuGly: 3.634 ± 0.315
0.917LeuHis: 0.917 ± 0.205
5.238LeuIle: 5.238 ± 0.555
6.122LeuLys: 6.122 ± 0.597
4.976LeuLeu: 4.976 ± 0.516
1.31LeuMet: 1.31 ± 0.219
6.45LeuAsn: 6.45 ± 0.581
2.816LeuPro: 2.816 ± 0.36
1.768LeuGln: 1.768 ± 0.231
3.012LeuArg: 3.012 ± 0.328
5.107LeuSer: 5.107 ± 0.58
3.798LeuThr: 3.798 ± 0.29
4.223LeuVal: 4.223 ± 0.359
0.884LeuTrp: 0.884 ± 0.149
3.634LeuTyr: 3.634 ± 0.402
0.0LeuXaa: 0.0 ± 0.0
Met
1.702MetAla: 1.702 ± 0.249
0.098MetCys: 0.098 ± 0.062
1.048MetAsp: 1.048 ± 0.145
1.08MetGlu: 1.08 ± 0.231
0.982MetPhe: 0.982 ± 0.229
0.622MetGly: 0.622 ± 0.149
0.131MetHis: 0.131 ± 0.067
1.866MetIle: 1.866 ± 0.279
1.473MetLys: 1.473 ± 0.238
1.408MetLeu: 1.408 ± 0.228
0.917MetMet: 0.917 ± 0.191
1.702MetAsn: 1.702 ± 0.216
1.179MetPro: 1.179 ± 0.224
0.589MetGln: 0.589 ± 0.118
0.982MetArg: 0.982 ± 0.233
0.917MetSer: 0.917 ± 0.18
1.408MetThr: 1.408 ± 0.246
1.015MetVal: 1.015 ± 0.172
0.065MetTrp: 0.065 ± 0.041
0.949MetTyr: 0.949 ± 0.203
0.0MetXaa: 0.0 ± 0.0
Asn
4.42AsnAla: 4.42 ± 0.475
0.786AsnCys: 0.786 ± 0.168
5.533AsnAsp: 5.533 ± 0.554
5.107AsnGlu: 5.107 ± 0.404
2.488AsnPhe: 2.488 ± 0.281
6.384AsnGly: 6.384 ± 0.461
1.375AsnHis: 1.375 ± 0.217
8.054AsnIle: 8.054 ± 0.725
7.497AsnLys: 7.497 ± 0.549
5.991AsnLeu: 5.991 ± 0.555
1.244AsnMet: 1.244 ± 0.206
8.512AsnAsn: 8.512 ± 0.658
3.372AsnPro: 3.372 ± 0.355
2.554AsnGln: 2.554 ± 0.391
3.077AsnArg: 3.077 ± 0.445
5.435AsnSer: 5.435 ± 0.539
5.238AsnThr: 5.238 ± 0.411
5.598AsnVal: 5.598 ± 0.51
1.08AsnTrp: 1.08 ± 0.221
4.583AsnTyr: 4.583 ± 0.439
0.0AsnXaa: 0.0 ± 0.0
Pro
1.801ProAla: 1.801 ± 0.299
0.229ProCys: 0.229 ± 0.095
1.833ProAsp: 1.833 ± 0.189
1.604ProGlu: 1.604 ± 0.211
1.342ProPhe: 1.342 ± 0.239
0.753ProGly: 0.753 ± 0.187
0.295ProHis: 0.295 ± 0.137
3.307ProIle: 3.307 ± 0.341
1.833ProLys: 1.833 ± 0.264
1.768ProLeu: 1.768 ± 0.219
0.36ProMet: 0.36 ± 0.148
2.488ProAsn: 2.488 ± 0.31
0.458ProPro: 0.458 ± 0.14
0.589ProGln: 0.589 ± 0.148
1.179ProArg: 1.179 ± 0.211
1.506ProSer: 1.506 ± 0.22
2.357ProThr: 2.357 ± 0.362
2.063ProVal: 2.063 ± 0.229
0.164ProTrp: 0.164 ± 0.072
1.604ProTyr: 1.604 ± 0.219
0.0ProXaa: 0.0 ± 0.0
Gln
2.226GlnAla: 2.226 ± 0.341
0.229GlnCys: 0.229 ± 0.1
1.964GlnAsp: 1.964 ± 0.303
1.801GlnGlu: 1.801 ± 0.304
1.277GlnPhe: 1.277 ± 0.178
1.833GlnGly: 1.833 ± 0.313
0.72GlnHis: 0.72 ± 0.135
2.357GlnIle: 2.357 ± 0.367
1.44GlnLys: 1.44 ± 0.238
2.914GlnLeu: 2.914 ± 0.34
0.524GlnMet: 0.524 ± 0.147
1.735GlnAsn: 1.735 ± 0.251
0.982GlnPro: 0.982 ± 0.22
1.539GlnGln: 1.539 ± 0.276
1.244GlnArg: 1.244 ± 0.189
2.03GlnSer: 2.03 ± 0.305
2.193GlnThr: 2.193 ± 0.27
1.67GlnVal: 1.67 ± 0.208
0.524GlnTrp: 0.524 ± 0.15
2.063GlnTyr: 2.063 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
2.193ArgAla: 2.193 ± 0.28
0.393ArgCys: 0.393 ± 0.159
2.717ArgAsp: 2.717 ± 0.272
2.324ArgGlu: 2.324 ± 0.317
1.833ArgPhe: 1.833 ± 0.211
2.816ArgGly: 2.816 ± 0.351
0.458ArgHis: 0.458 ± 0.138
3.176ArgIle: 3.176 ± 0.321
3.339ArgLys: 3.339 ± 0.462
3.077ArgLeu: 3.077 ± 0.354
0.786ArgMet: 0.786 ± 0.187
3.405ArgAsn: 3.405 ± 0.421
0.655ArgPro: 0.655 ± 0.138
1.408ArgGln: 1.408 ± 0.233
1.932ArgArg: 1.932 ± 0.329
2.128ArgSer: 2.128 ± 0.309
2.685ArgThr: 2.685 ± 0.363
2.685ArgVal: 2.685 ± 0.262
0.458ArgTrp: 0.458 ± 0.102
2.652ArgTyr: 2.652 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
3.798SerAla: 3.798 ± 0.789
0.426SerCys: 0.426 ± 0.107
3.699SerAsp: 3.699 ± 0.358
3.11SerGlu: 3.11 ± 0.35
2.259SerPhe: 2.259 ± 0.353
3.896SerGly: 3.896 ± 0.978
0.949SerHis: 0.949 ± 0.146
5.631SerIle: 5.631 ± 0.611
4.845SerLys: 4.845 ± 0.461
3.896SerLeu: 3.896 ± 0.31
1.375SerMet: 1.375 ± 0.219
5.074SerAsn: 5.074 ± 0.37
1.408SerPro: 1.408 ± 0.192
1.899SerGln: 1.899 ± 0.262
2.914SerArg: 2.914 ± 0.38
3.765SerSer: 3.765 ± 0.518
3.634SerThr: 3.634 ± 0.634
3.536SerVal: 3.536 ± 0.452
0.557SerTrp: 0.557 ± 0.192
3.274SerTyr: 3.274 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
3.536ThrAla: 3.536 ± 0.406
0.753ThrCys: 0.753 ± 0.159
3.699ThrAsp: 3.699 ± 0.296
3.569ThrGlu: 3.569 ± 0.384
2.292ThrPhe: 2.292 ± 0.271
3.798ThrGly: 3.798 ± 0.375
0.655ThrHis: 0.655 ± 0.127
5.893ThrIle: 5.893 ± 0.667
4.06ThrLys: 4.06 ± 0.332
3.438ThrLeu: 3.438 ± 0.544
1.015ThrMet: 1.015 ± 0.197
5.107ThrAsn: 5.107 ± 0.4
2.226ThrPro: 2.226 ± 0.316
2.193ThrGln: 2.193 ± 0.26
2.717ThrArg: 2.717 ± 0.233
4.256ThrSer: 4.256 ± 0.732
3.274ThrThr: 3.274 ± 0.518
4.289ThrVal: 4.289 ± 0.672
0.917ThrTrp: 0.917 ± 0.381
3.503ThrTyr: 3.503 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
3.438ValAla: 3.438 ± 0.347
0.557ValCys: 0.557 ± 0.176
4.256ValAsp: 4.256 ± 0.299
4.06ValGlu: 4.06 ± 0.366
2.259ValPhe: 2.259 ± 0.312
3.143ValGly: 3.143 ± 0.289
1.015ValHis: 1.015 ± 0.181
5.631ValIle: 5.631 ± 0.624
4.289ValLys: 4.289 ± 0.384
4.06ValLeu: 4.06 ± 0.369
1.244ValMet: 1.244 ± 0.243
5.467ValAsn: 5.467 ± 0.594
2.161ValPro: 2.161 ± 0.231
1.539ValGln: 1.539 ± 0.249
2.161ValArg: 2.161 ± 0.219
3.405ValSer: 3.405 ± 0.529
3.601ValThr: 3.601 ± 0.565
3.569ValVal: 3.569 ± 0.386
0.655ValTrp: 0.655 ± 0.195
3.241ValTyr: 3.241 ± 0.514
0.0ValXaa: 0.0 ± 0.0
Trp
0.524TrpAla: 0.524 ± 0.124
0.229TrpCys: 0.229 ± 0.102
0.557TrpAsp: 0.557 ± 0.151
0.786TrpGlu: 0.786 ± 0.143
0.622TrpPhe: 0.622 ± 0.129
0.557TrpGly: 0.557 ± 0.114
0.164TrpHis: 0.164 ± 0.062
0.786TrpIle: 0.786 ± 0.175
0.917TrpLys: 0.917 ± 0.168
0.589TrpLeu: 0.589 ± 0.137
0.262TrpMet: 0.262 ± 0.096
1.113TrpAsn: 1.113 ± 0.316
0.033TrpPro: 0.033 ± 0.037
0.491TrpGln: 0.491 ± 0.112
0.491TrpArg: 0.491 ± 0.125
0.851TrpSer: 0.851 ± 0.293
1.08TrpThr: 1.08 ± 0.308
0.557TrpVal: 0.557 ± 0.138
0.131TrpTrp: 0.131 ± 0.057
0.393TrpTyr: 0.393 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.833TyrAla: 1.833 ± 0.237
0.688TyrCys: 0.688 ± 0.158
3.405TyrAsp: 3.405 ± 0.244
2.03TyrGlu: 2.03 ± 0.231
1.899TyrPhe: 1.899 ± 0.294
3.208TyrGly: 3.208 ± 0.37
1.211TyrHis: 1.211 ± 0.194
5.467TyrIle: 5.467 ± 0.507
4.747TyrLys: 4.747 ± 0.468
3.732TyrLeu: 3.732 ± 0.352
1.015TyrMet: 1.015 ± 0.188
5.697TyrAsn: 5.697 ± 0.539
1.473TyrPro: 1.473 ± 0.361
1.44TyrGln: 1.44 ± 0.241
2.226TyrArg: 2.226 ± 0.264
2.946TyrSer: 2.946 ± 0.359
3.634TyrThr: 3.634 ± 0.451
3.045TyrVal: 3.045 ± 0.293
0.491TyrTrp: 0.491 ± 0.137
2.488TyrTyr: 2.488 ± 0.338
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (30546 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski