Acidiluteibacter ferrifornacis
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3010 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6N9NN41|A0A6N9NN41_9FLAO Methyltransferase domain-containing protein OS=Acidiluteibacter ferrifornacis OX=2692424 GN=GQN54_10940 PE=4 SV=1
MM1 pKa = 7.82 KK2 pKa = 10.32 KK3 pKa = 10.06 RR4 pKa = 11.84 LLSVGLLLLSFAGYY18 pKa = 9.06 SQIFLDD24 pKa = 4.12 EE25 pKa = 4.57 DD26 pKa = 4.12 FEE28 pKa = 4.88 TFTVGAAGPTFANGWTNSGTTTPRR52 pKa = 11.84 WEE54 pKa = 4.15 VEE56 pKa = 4.09 DD57 pKa = 3.4 ATGANEE63 pKa = 3.86 NSLATGPFFDD73 pKa = 3.94 ATSPTVAGGKK83 pKa = 9.28 YY84 pKa = 10.19 LYY86 pKa = 10.83 LEE88 pKa = 4.39 TSGGALNDD96 pKa = 3.55 QNTLSSPPVSIPMSATGVKK115 pKa = 10.23 LDD117 pKa = 3.91 FAYY120 pKa = 10.78 HH121 pKa = 5.5 MFGASMGEE129 pKa = 4.08 LYY131 pKa = 10.97 VVIDD135 pKa = 3.97 TNNVSDD141 pKa = 4.16 TVVTLIGQQQTSGADD156 pKa = 3.42 PFITSSTILTGYY168 pKa = 9.59 AGKK171 pKa = 8.28 TLTVKK176 pKa = 10.68 FIGKK180 pKa = 9.85 RR181 pKa = 11.84 GTSYY185 pKa = 10.5 TGDD188 pKa = 3.09 ISIDD192 pKa = 3.67 EE193 pKa = 4.5 VKK195 pKa = 10.64 LFQPVPNEE203 pKa = 3.74 MGITEE208 pKa = 4.22 ITTPTSSCGLTASEE222 pKa = 4.48 VVTIEE227 pKa = 3.82 VQNFGTSNITSFDD240 pKa = 3.15 ASFSINGGTPVTEE253 pKa = 4.34 TVTTTITPNSIYY265 pKa = 10.33 SYY267 pKa = 10.23 TFTATANLSAFGPYY281 pKa = 8.35 EE282 pKa = 3.93 VKK284 pKa = 10.71 SYY286 pKa = 9.3 VTLLNDD292 pKa = 3.59 PTSSNDD298 pKa = 3.56 TLVKK302 pKa = 10.67 NITNIPILNGFPYY315 pKa = 10.98 LEE317 pKa = 4.35 TFEE320 pKa = 5.02 SGPGGWISGGTNSSWALGTPAGTVINSAAGGTNSWVTNLTGTYY363 pKa = 9.72 NATEE367 pKa = 3.81 ASYY370 pKa = 11.27 VVSPCFDD377 pKa = 3.53 FTSLVAPVFSADD389 pKa = 3.31 VWWNSEE395 pKa = 3.92 FSWDD399 pKa = 3.51 GAILQTSIDD408 pKa = 5.21 GGTTWQKK415 pKa = 10.93 VGANGDD421 pKa = 3.78 PNNWYY426 pKa = 8.91 TDD428 pKa = 3.48 NTISGLTPLAPSQEE442 pKa = 4.01 GWTGRR447 pKa = 11.84 NSTSNGSGGWVKK459 pKa = 10.63 VKK461 pKa = 10.94 APLTGLGSVSGVILRR476 pKa = 11.84 IAFGSDD482 pKa = 2.8 GSVMDD487 pKa = 4.46 EE488 pKa = 4.63 GFAFDD493 pKa = 5.37 NIQIYY498 pKa = 7.61 DD499 pKa = 3.89 TPAQDD504 pKa = 5.29 AEE506 pKa = 4.5 LLSITSPINGCGLTSTEE523 pKa = 3.88 QAEE526 pKa = 4.41 VQIVNAGSASISNFPVSITVNGGTPLTEE554 pKa = 4.07 TVTATILPLDD564 pKa = 3.57 TLFYY568 pKa = 10.48 TFTGTVNLSTTGNYY582 pKa = 9.82 SIKK585 pKa = 10.36 AYY587 pKa = 9.67 TGLTGDD593 pKa = 4.51 GLSSNDD599 pKa = 3.2 TATKK603 pKa = 10.25 VVTHH607 pKa = 6.6 IPIISTFPYY616 pKa = 8.68 STSFEE621 pKa = 4.14 NSNEE625 pKa = 3.69 GWLSSGINNSWSLGVPAGSIIDD647 pKa = 3.76 TASNGTKK654 pKa = 10.2 AWVTNLNGVYY664 pKa = 10.59 NNNEE668 pKa = 3.57 QSFVVSPCLDD678 pKa = 3.58 FTNLTSPLISLDD690 pKa = 3.15 IQYY693 pKa = 10.99 EE694 pKa = 4.15 LDD696 pKa = 3.48 NSNDD700 pKa = 3.32 GVVLQSSIDD709 pKa = 4.49 GGATWQKK716 pKa = 10.43 VGAFGSPNNWYY727 pKa = 8.87 TDD729 pKa = 3.37 NTIVGLSSIEE739 pKa = 3.95 TSQEE743 pKa = 3.12 GWTGSGTSGSNGWKK757 pKa = 9.51 SARR760 pKa = 11.84 AALSGLGGEE769 pKa = 4.38 PSVILRR775 pKa = 11.84 IALGTNGFTTFEE787 pKa = 4.07 GFAFDD792 pKa = 4.18 NVTIQEE798 pKa = 4.43 APSTDD803 pKa = 3.16 AGVVDD808 pKa = 5.68 IIDD811 pKa = 4.08 PASGCGLSSAEE822 pKa = 4.12 VVTVRR827 pKa = 11.84 IVNDD831 pKa = 3.62 GATNISNFPVSVVLNNGTPLTEE853 pKa = 4.2 TVTATMLPNDD863 pKa = 3.71 TLTYY867 pKa = 10.32 TFTGTLNMSALGTYY881 pKa = 10.1 SVVSYY886 pKa = 10.53 TSLPLDD892 pKa = 3.59 GFNVNDD898 pKa = 4.0 TASKK902 pKa = 8.75 TVVNIPVISGFPYY915 pKa = 9.56 TEE917 pKa = 4.08 SFEE920 pKa = 4.28 TSAGGWTVDD929 pKa = 3.24 GGTSSFALGAPIGSLINSASNGTQAWVTNLSGNYY963 pKa = 9.49 LADD966 pKa = 3.23 EE967 pKa = 4.58 AGYY970 pKa = 10.77 VKK972 pKa = 10.55 SPCLDD977 pKa = 3.8 FSTLIAPIISMDD989 pKa = 3.44 VWWNSEE995 pKa = 3.95 FSWDD999 pKa = 3.6 GAVLQSSIDD1008 pKa = 4.72 GGTTWQKK1015 pKa = 10.65 VGSFGDD1021 pKa = 3.58 PNNWYY1026 pKa = 10.18 NDD1028 pKa = 3.16 NSINGLNTGSPILEE1042 pKa = 4.11 PSGEE1046 pKa = 3.97 GWTGRR1051 pKa = 11.84 NSSSNGSNGWVNATHH1066 pKa = 6.51 QLTGLGGQPGVIFRR1080 pKa = 11.84 VAFGSDD1086 pKa = 3.14 GSVMDD1091 pKa = 4.46 EE1092 pKa = 4.54 GFAFDD1097 pKa = 3.9 NVQIYY1102 pKa = 7.96 EE1103 pKa = 4.31 SPAVDD1108 pKa = 3.61 IKK1110 pKa = 11.43 AMEE1113 pKa = 4.05 VSRR1116 pKa = 11.84 PLVGCGLSATEE1127 pKa = 4.48 TIAARR1132 pKa = 11.84 YY1133 pKa = 7.53 EE1134 pKa = 4.02 NLGTDD1139 pKa = 3.42 TLTNFPVAYY1148 pKa = 9.39 SVNGVAITPEE1158 pKa = 4.3 TFTDD1162 pKa = 3.59 TLFPGDD1168 pKa = 3.45 KK1169 pKa = 9.91 KK1170 pKa = 11.09 SYY1172 pKa = 10.65 EE1173 pKa = 4.14 FTTTANLSSTIQYY1186 pKa = 9.18 TIEE1189 pKa = 3.93 VWSAAAGDD1197 pKa = 4.9 AITSNDD1203 pKa = 3.54 TASVTFTNIAASTNPYY1219 pKa = 9.13 PQTFDD1224 pKa = 3.48 VLTDD1228 pKa = 3.71 GATDD1232 pKa = 3.85 FSPINWTPINAGAFDD1247 pKa = 3.72 WRR1249 pKa = 11.84 AEE1251 pKa = 4.15 TT1252 pKa = 4.54
Molecular weight: 131.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.935
Patrickios 0.909
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A6N9NJ49|A0A6N9NJ49_9FLAO DUF4834 family protein OS=Acidiluteibacter ferrifornacis OX=2692424 GN=GQN54_07115 PE=4 SV=1
MM1 pKa = 7.19 IQQEE5 pKa = 4.43 SRR7 pKa = 11.84 LSVADD12 pKa = 3.32 NSGAKK17 pKa = 9.22 EE18 pKa = 3.93 VLCIRR23 pKa = 11.84 VLGGTGKK30 pKa = 10.44 RR31 pKa = 11.84 YY32 pKa = 10.3 ARR34 pKa = 11.84 IGDD37 pKa = 3.98 KK38 pKa = 10.52 IVVSVKK44 pKa = 10.53 SAIPSGNIKK53 pKa = 10.12 KK54 pKa = 10.34 GAVSKK59 pKa = 10.89 AVVVRR64 pKa = 11.84 TRR66 pKa = 11.84 KK67 pKa = 9.57 EE68 pKa = 3.23 IRR70 pKa = 11.84 RR71 pKa = 11.84 PDD73 pKa = 3.16 GSYY76 pKa = 10.62 IRR78 pKa = 11.84 FDD80 pKa = 3.58 NNAVVLLNAAGEE92 pKa = 4.21 MRR94 pKa = 11.84 GTRR97 pKa = 11.84 IFGPVARR104 pKa = 11.84 EE105 pKa = 3.54 LRR107 pKa = 11.84 DD108 pKa = 3.52 RR109 pKa = 11.84 QFMKK113 pKa = 10.24 IVSLAPEE120 pKa = 4.12 VLL122 pKa = 3.56
Molecular weight: 13.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.706
IPC_protein 10.54
Toseland 10.965
ProMoST 10.716
Dawson 11.023
Bjellqvist 10.73
Wikipedia 11.242
Rodwell 11.242
Grimsley 11.052
Solomon 11.199
Lehninger 11.169
Nozaki 10.935
DTASelect 10.73
Thurlkill 10.95
EMBOSS 11.374
Sillero 10.965
Patrickios 10.979
IPC_peptide 11.213
IPC2_peptide 9.648
IPC2.peptide.svr19 8.704
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3010
0
3010
1071256
27
3996
355.9
40.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.337 ± 0.056
0.793 ± 0.019
5.372 ± 0.041
6.467 ± 0.056
5.236 ± 0.041
6.461 ± 0.052
1.686 ± 0.021
8.146 ± 0.045
7.221 ± 0.067
9.23 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.157 ± 0.025
6.216 ± 0.053
3.405 ± 0.028
3.526 ± 0.029
3.423 ± 0.032
7.172 ± 0.049
5.699 ± 0.077
6.205 ± 0.038
1.051 ± 0.019
4.196 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here