Cabbage cytorhabdovirus 1
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2PYM8|A0A2D2PYM8_9RHAB Matrix protein OS=Cabbage cytorhabdovirus 1 OX=2051550 PE=4 SV=1
MM1 pKa = 8.2 DD2 pKa = 5.15 PQQSAIDD9 pKa = 3.93 FSEE12 pKa = 4.04 IDD14 pKa = 3.77 ANLHH18 pKa = 5.41 RR19 pKa = 11.84 SPYY22 pKa = 9.77 VVPALDD28 pKa = 4.57 DD29 pKa = 4.8 DD30 pKa = 4.14 MDD32 pKa = 5.31 GGEE35 pKa = 4.44 IVQDD39 pKa = 3.8 DD40 pKa = 4.33 LSVPRR45 pKa = 11.84 STDD48 pKa = 3.03 DD49 pKa = 3.82 VAPPTQKK56 pKa = 10.69 SSLVSSEE63 pKa = 4.07 VVEE66 pKa = 4.24 SLLYY70 pKa = 9.76 EE71 pKa = 4.06 ASVLHH76 pKa = 6.67 GIAVTPHH83 pKa = 6.12 MSTTAIALAHH93 pKa = 6.3 NIGLEE98 pKa = 4.01 PHH100 pKa = 6.04 SLDD103 pKa = 3.04 WFLAGISYY111 pKa = 10.83 ANNSMIVEE119 pKa = 4.27 KK120 pKa = 10.49 LSSIIKK126 pKa = 10.22 DD127 pKa = 3.45 MQIEE131 pKa = 4.31 TRR133 pKa = 11.84 NLQTATSSVAAVSNEE148 pKa = 3.48 FLGKK152 pKa = 9.22 MSRR155 pKa = 11.84 NKK157 pKa = 10.45 RR158 pKa = 11.84 EE159 pKa = 4.52 IIDD162 pKa = 3.57 EE163 pKa = 4.15 MEE165 pKa = 4.04 KK166 pKa = 9.78 TRR168 pKa = 11.84 EE169 pKa = 4.04 SVLNAVASIQSAEE182 pKa = 4.19 LGSHH186 pKa = 6.62 IGDD189 pKa = 3.74 DD190 pKa = 4.26 FVNLTVNPEE199 pKa = 4.08 GTSEE203 pKa = 4.28 RR204 pKa = 11.84 STVPPPAPVDD214 pKa = 3.33 AGLLSKK220 pKa = 10.77 VLSNPVTLKK229 pKa = 11.03 SPDD232 pKa = 3.5 EE233 pKa = 3.54 ILYY236 pKa = 9.68 ARR238 pKa = 11.84 KK239 pKa = 9.93 YY240 pKa = 10.84 KK241 pKa = 10.77 LLLDD245 pKa = 4.55 LGIEE249 pKa = 3.97 IPKK252 pKa = 10.69 ANLSPDD258 pKa = 2.95 IMNTLIPDD266 pKa = 3.82 WQLEE270 pKa = 4.08 AAEE273 pKa = 5.08 AGLDD277 pKa = 3.59 SKK279 pKa = 10.12 TKK281 pKa = 10.09 MEE283 pKa = 4.49 LTDD286 pKa = 3.78 EE287 pKa = 4.63 LLDD290 pKa = 4.34 IVITLNLII298 pKa = 3.82
Molecular weight: 32.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.471
IPC2_protein 4.317
IPC_protein 4.291
Toseland 4.113
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.139
Rodwell 4.126
Grimsley 4.024
Solomon 4.253
Lehninger 4.202
Nozaki 4.355
DTASelect 4.546
Thurlkill 4.126
EMBOSS 4.151
Sillero 4.406
Patrickios 3.948
IPC_peptide 4.253
IPC2_peptide 4.393
IPC2.peptide.svr19 4.332
Protein with the highest isoelectric point:
>tr|A0A2D2PYL4|A0A2D2PYL4_9RHAB Glycoprotein OS=Cabbage cytorhabdovirus 1 OX=2051550 PE=4 SV=1
MM1 pKa = 7.58 CLSCLKK7 pKa = 10.28 YY8 pKa = 10.72 VWNLCLTMCDD18 pKa = 3.07 LRR20 pKa = 11.84 KK21 pKa = 9.62 LVRR24 pKa = 11.84 VTMINVNDD32 pKa = 3.49 VRR34 pKa = 11.84 KK35 pKa = 9.98 QIIKK39 pKa = 10.51 SGSLTSAVGTGYY51 pKa = 11.1 VYY53 pKa = 10.84 DD54 pKa = 4.05 GSYY57 pKa = 11.05 NKK59 pKa = 10.03 YY60 pKa = 9.56 ARR62 pKa = 11.84 KK63 pKa = 9.7 RR64 pKa = 11.84 EE65 pKa = 3.97 LNLRR69 pKa = 11.84 VSSSGDD75 pKa = 3.14 KK76 pKa = 10.56 NIMMRR81 pKa = 11.84 HH82 pKa = 4.69 VPIFDD87 pKa = 5.11 DD88 pKa = 3.67 EE89 pKa = 4.4 DD90 pKa = 3.6 LRR92 pKa = 11.84 ALRR95 pKa = 11.84 SEE97 pKa = 4.29 ANSNKK102 pKa = 9.82 YY103 pKa = 9.21 IHH105 pKa = 6.85 IGCLTISIEE114 pKa = 4.05 PLMHH118 pKa = 6.58 KK119 pKa = 10.08 RR120 pKa = 11.84 YY121 pKa = 9.07 MDD123 pKa = 4.17 LYY125 pKa = 8.05 GDD127 pKa = 3.9 KK128 pKa = 10.17 MRR130 pKa = 11.84 GICAVIDD137 pKa = 3.52 TTFADD142 pKa = 3.95 PAEE145 pKa = 4.84 SIISAHH151 pKa = 6.8 RR152 pKa = 11.84 YY153 pKa = 7.26 EE154 pKa = 4.35 LSKK157 pKa = 11.12 GRR159 pKa = 11.84 ADD161 pKa = 3.67 FVSMPNHH168 pKa = 6.43 CLSLLDD174 pKa = 4.42 PNLKK178 pKa = 9.67 QRR180 pKa = 11.84 LSVMISLDD188 pKa = 3.9 GINVKK193 pKa = 10.21 KK194 pKa = 10.34 GNEE197 pKa = 3.97 MFNVCIGYY205 pKa = 9.47 IVTGVNTLNPTKK217 pKa = 10.15 AVSMSNIPITGTSEE231 pKa = 4.26 CEE233 pKa = 3.9 PGDD236 pKa = 3.69 LSEE239 pKa = 6.36 DD240 pKa = 3.29 MLEE243 pKa = 4.66 GIKK246 pKa = 10.69 GSNGNLLISHH256 pKa = 7.42 DD257 pKa = 4.38 PSDD260 pKa = 4.14 DD261 pKa = 4.32 DD262 pKa = 5.47 IYY264 pKa = 10.96 IKK266 pKa = 10.94 SKK268 pKa = 11.04 GSFLSNLSGKK278 pKa = 7.76 TKK280 pKa = 9.92 IIKK283 pKa = 9.74 RR284 pKa = 11.84 RR285 pKa = 11.84 TMRR288 pKa = 11.84 AKK290 pKa = 10.51 PIDD293 pKa = 3.74 ITPADD298 pKa = 3.76 HH299 pKa = 6.66 NSNVRR304 pKa = 11.84 SISSDD309 pKa = 2.85 EE310 pKa = 4.01 HH311 pKa = 7.81 LGEE314 pKa = 4.18 SEE316 pKa = 4.55 RR317 pKa = 11.84 RR318 pKa = 11.84 SCSKK322 pKa = 10.41 ISASDD327 pKa = 3.98 CYY329 pKa = 9.89 QTLNHH334 pKa = 5.89 QAVLKK339 pKa = 10.25 RR340 pKa = 11.84 AMSSRR345 pKa = 11.84 YY346 pKa = 9.13 GG347 pKa = 3.31
Molecular weight: 38.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.696
IPC2_protein 7.936
IPC_protein 7.775
Toseland 7.41
ProMoST 8.346
Dawson 8.507
Bjellqvist 8.843
Wikipedia 8.434
Rodwell 8.536
Grimsley 7.41
Solomon 8.595
Lehninger 8.595
Nozaki 9.077
DTASelect 8.565
Thurlkill 8.639
EMBOSS 8.712
Sillero 8.96
Patrickios 4.355
IPC_peptide 8.58
IPC2_peptide 7.79
IPC2.peptide.svr19 7.929
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3919
168
2071
653.2
73.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.945 ± 0.953
1.863 ± 0.421
5.639 ± 0.466
5.588 ± 0.443
3.292 ± 0.431
5.537 ± 0.262
2.297 ± 0.152
6.405 ± 0.343
6.073 ± 0.543
10.564 ± 0.801
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.343 ± 0.196
4.925 ± 0.284
4.772 ± 0.274
2.679 ± 0.386
5.41 ± 0.417
8.497 ± 0.824
5.716 ± 0.444
6.634 ± 0.451
1.531 ± 0.286
3.292 ± 0.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here