Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99) (ISAV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Isavirus; Salmon isavirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8V3U2|NS1_ISAV8 Non-structural protein 1 OS=Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99) OX=652965 GN=Segment-7 PE=4 SV=1
MM1 pKa = 7.44NLLLLLQVASFLSDD15 pKa = 3.19SKK17 pKa = 11.8VPGEE21 pKa = 5.17DD22 pKa = 3.39GTSSTSGMLDD32 pKa = 3.61LLRR35 pKa = 11.84DD36 pKa = 3.77QVDD39 pKa = 3.62SLSINDD45 pKa = 3.7STTEE49 pKa = 3.78PKK51 pKa = 9.8TRR53 pKa = 11.84LDD55 pKa = 3.52PGLYY59 pKa = 9.38PWLKK63 pKa = 7.7WTEE66 pKa = 3.69TAYY69 pKa = 10.61RR70 pKa = 11.84SSTRR74 pKa = 11.84SLASTIVMGALVQQRR89 pKa = 11.84GSGNGITMRR98 pKa = 11.84EE99 pKa = 4.14LEE101 pKa = 4.67LSLGLDD107 pKa = 3.83FTSEE111 pKa = 4.25CDD113 pKa = 3.26WLKK116 pKa = 9.58TCYY119 pKa = 10.13VNKK122 pKa = 10.35NFVFLSEE129 pKa = 4.45KK130 pKa = 10.21EE131 pKa = 3.84IAVNMEE137 pKa = 3.8VEE139 pKa = 4.59KK140 pKa = 10.32FICNEE145 pKa = 3.63NN146 pKa = 3.3

Molecular weight: 16.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8V3T7|NCAP_ISAV8 Nucleoprotein OS=Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99) OX=652965 GN=Segment-3 PE=1 SV=1
MM1 pKa = 6.71EE2 pKa = 4.7TLVGGLLTGEE12 pKa = 4.92DD13 pKa = 4.05SLISMSNDD21 pKa = 2.95VSCLYY26 pKa = 10.87VYY28 pKa = 10.04DD29 pKa = 3.75GPMRR33 pKa = 11.84VFSQNALMPTLQSVKK48 pKa = 10.46RR49 pKa = 11.84SDD51 pKa = 3.35QFSKK55 pKa = 11.07GKK57 pKa = 7.77TKK59 pKa = 10.7RR60 pKa = 11.84FIIDD64 pKa = 3.73LFGMKK69 pKa = 10.19RR70 pKa = 11.84MWDD73 pKa = 3.15IGNKK77 pKa = 7.83QLEE80 pKa = 4.44DD81 pKa = 3.73EE82 pKa = 4.77NLDD85 pKa = 3.68EE86 pKa = 4.55TVGVADD92 pKa = 4.99LGLVKK97 pKa = 10.61YY98 pKa = 10.05LINNKK103 pKa = 9.32YY104 pKa = 10.67DD105 pKa = 3.72EE106 pKa = 4.64AEE108 pKa = 4.08KK109 pKa = 10.18TSLRR113 pKa = 11.84KK114 pKa = 10.15SMEE117 pKa = 3.84EE118 pKa = 3.67AFEE121 pKa = 4.32KK122 pKa = 11.0SMNEE126 pKa = 3.68EE127 pKa = 3.51FVVLNKK133 pKa = 10.45GKK135 pKa = 10.03SANDD139 pKa = 3.57IISDD143 pKa = 3.82TNAMCKK149 pKa = 10.23FCVKK153 pKa = 9.84NWIVATGFRR162 pKa = 11.84GRR164 pKa = 11.84TMSDD168 pKa = 3.47LIEE171 pKa = 3.88HH172 pKa = 7.41HH173 pKa = 6.37FRR175 pKa = 11.84CMQGKK180 pKa = 9.06QEE182 pKa = 3.99VKK184 pKa = 10.51GYY186 pKa = 9.05IWKK189 pKa = 9.79HH190 pKa = 5.44KK191 pKa = 9.72YY192 pKa = 9.12NEE194 pKa = 3.93RR195 pKa = 11.84LKK197 pKa = 10.7RR198 pKa = 11.84KK199 pKa = 8.68QLSKK203 pKa = 11.48EE204 pKa = 3.75EE205 pKa = 4.07VKK207 pKa = 10.5FDD209 pKa = 3.54RR210 pKa = 11.84EE211 pKa = 4.49EE212 pKa = 3.89YY213 pKa = 8.45TSRR216 pKa = 11.84SFRR219 pKa = 11.84LLSFLKK225 pKa = 10.52NSEE228 pKa = 4.1RR229 pKa = 11.84TKK231 pKa = 10.83LEE233 pKa = 3.52PRR235 pKa = 11.84AVFTAGVPWRR245 pKa = 11.84AFIFVLEE252 pKa = 4.05QTMLVVNKK260 pKa = 9.88LDD262 pKa = 4.1PNSVIWMGSDD272 pKa = 3.33AKK274 pKa = 10.97INTTNSRR281 pKa = 11.84IKK283 pKa = 10.29EE284 pKa = 3.77IGMKK288 pKa = 10.28NQGQTLVTLTGDD300 pKa = 3.04NSKK303 pKa = 10.79YY304 pKa = 10.18NEE306 pKa = 4.27SMCPEE311 pKa = 3.7VMMVFLRR318 pKa = 11.84EE319 pKa = 4.09LGIKK323 pKa = 10.48GPMLEE328 pKa = 4.2VLDD331 pKa = 3.82YY332 pKa = 11.42ALWQFSQKK340 pKa = 8.96SVKK343 pKa = 9.71PVAPIKK349 pKa = 10.64KK350 pKa = 8.05RR351 pKa = 11.84TGKK354 pKa = 8.28STVVIKK360 pKa = 10.74ADD362 pKa = 3.55SVKK365 pKa = 10.36EE366 pKa = 3.81CRR368 pKa = 11.84DD369 pKa = 3.23AFNEE373 pKa = 4.25KK374 pKa = 9.95EE375 pKa = 4.07LEE377 pKa = 4.54LIQGVEE383 pKa = 3.73WMDD386 pKa = 3.69DD387 pKa = 3.0GFVRR391 pKa = 11.84VRR393 pKa = 11.84RR394 pKa = 11.84GMLMGMANNAFTTASTIASSFSFTPEE420 pKa = 3.34AVYY423 pKa = 9.53TLQSSDD429 pKa = 3.85DD430 pKa = 4.17FVTGSCGRR438 pKa = 11.84DD439 pKa = 3.32VQHH442 pKa = 6.72ARR444 pKa = 11.84QRR446 pKa = 11.84LEE448 pKa = 3.68MALKK452 pKa = 9.45VSKK455 pKa = 10.64AAGLNVSQKK464 pKa = 10.48KK465 pKa = 9.77SFYY468 pKa = 10.8VEE470 pKa = 3.68GTTFEE475 pKa = 4.31FNSMFVRR482 pKa = 11.84DD483 pKa = 4.43GKK485 pKa = 11.47VMANGGNFEE494 pKa = 4.38NMTVPGGLGPSTDD507 pKa = 3.34LFVVGKK513 pKa = 7.41QARR516 pKa = 11.84NSMLRR521 pKa = 11.84GNLSFSQAMEE531 pKa = 3.78MCKK534 pKa = 9.92IGITNVEE541 pKa = 3.68KK542 pKa = 10.78VYY544 pKa = 10.92YY545 pKa = 10.35GNRR548 pKa = 11.84KK549 pKa = 7.82YY550 pKa = 10.61QEE552 pKa = 4.1LKK554 pKa = 10.25NEE556 pKa = 3.71IRR558 pKa = 11.84EE559 pKa = 4.29KK560 pKa = 10.79CGEE563 pKa = 4.03EE564 pKa = 4.05TMSIPEE570 pKa = 4.06SMGGDD575 pKa = 3.35RR576 pKa = 11.84KK577 pKa = 9.75PRR579 pKa = 11.84PWEE582 pKa = 3.91LPQSFDD588 pKa = 4.4GIALKK593 pKa = 10.51EE594 pKa = 4.04AVNRR598 pKa = 11.84GHH600 pKa = 6.93WKK602 pKa = 7.77AAKK605 pKa = 9.64YY606 pKa = 10.07IKK608 pKa = 9.9SCCSIEE614 pKa = 3.84FDD616 pKa = 4.94EE617 pKa = 5.93EE618 pKa = 5.1GDD620 pKa = 4.16QSWDD624 pKa = 3.22TSKK627 pKa = 9.73TALVVIRR634 pKa = 11.84KK635 pKa = 9.35NEE637 pKa = 3.46TDD639 pKa = 2.89MRR641 pKa = 11.84RR642 pKa = 11.84RR643 pKa = 11.84TVKK646 pKa = 9.35TRR648 pKa = 11.84NPKK651 pKa = 10.58DD652 pKa = 3.68KK653 pKa = 10.77IFNDD657 pKa = 3.27AMNKK661 pKa = 8.56AKK663 pKa = 10.38RR664 pKa = 11.84MYY666 pKa = 8.79EE667 pKa = 4.05TVVDD671 pKa = 4.64RR672 pKa = 11.84NPLLGLKK679 pKa = 10.29GKK681 pKa = 10.3GGRR684 pKa = 11.84LTVKK688 pKa = 10.13DD689 pKa = 3.81LKK691 pKa = 10.87ARR693 pKa = 11.84KK694 pKa = 9.55LIDD697 pKa = 3.32EE698 pKa = 4.47VEE700 pKa = 4.3VVKK703 pKa = 10.76KK704 pKa = 10.36KK705 pKa = 10.56KK706 pKa = 9.93HH707 pKa = 3.95VV708 pKa = 3.3

Molecular weight: 80.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

4281

146

722

428.1

47.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.7 ± 0.48

2.453 ± 0.353

5.466 ± 0.427

7.452 ± 0.509

3.854 ± 0.363

8.339 ± 0.574

1.145 ± 0.284

4.999 ± 0.451

7.381 ± 0.531

7.779 ± 0.408

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.018 ± 0.377

4.205 ± 0.429

3.107 ± 0.279

3.2 ± 0.212

5.91 ± 0.523

7.381 ± 0.48

6.657 ± 0.434

7.265 ± 0.419

1.355 ± 0.171

2.219 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski