Draconibacterium orientale
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3940 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X5DM66|X5DM66_9BACT Hemoglobin/transferrin/lactoferrin receptor protein OS=Draconibacterium orientale OX=1168034 GN=FH5T_06340 PE=3 SV=1
MM1 pKa = 7.61 SIYY4 pKa = 10.44 GYY6 pKa = 9.38 SKK8 pKa = 10.95 KK9 pKa = 10.64 LVFLFAMVLIAGFVMAQKK27 pKa = 7.67 TTTLVQSGNSNIATVDD43 pKa = 3.31 QMACLSGAVNSIFATQSGNWNTLTTFQKK71 pKa = 10.81 GWDD74 pKa = 3.72 NNIDD78 pKa = 3.79 LLQSGDD84 pKa = 3.85 HH85 pKa = 5.95 NTADD89 pKa = 3.88 MTQEE93 pKa = 3.75 TCDD96 pKa = 3.68 YY97 pKa = 10.79 DD98 pKa = 4.84 DD99 pKa = 3.34 SHH101 pKa = 7.9 GYY103 pKa = 7.97 NTAKK107 pKa = 10.08 VVQYY111 pKa = 11.54 GDD113 pKa = 3.89 GNTANLSQMEE123 pKa = 4.4 EE124 pKa = 4.18 DD125 pKa = 4.94 GGLADD130 pKa = 3.92 IDD132 pKa = 4.46 YY133 pKa = 11.11 VGGWLPLPILPEE145 pKa = 3.96 RR146 pKa = 11.84 DD147 pKa = 3.1 RR148 pKa = 11.84 SINEE152 pKa = 3.61 ATTTQSGNSNTYY164 pKa = 9.92 NLAQGRR170 pKa = 11.84 EE171 pKa = 4.02 NYY173 pKa = 10.03 IPDD176 pKa = 3.36 NTSYY180 pKa = 9.83 LTQSGNSNAADD191 pKa = 3.26 IDD193 pKa = 3.62 QLGFNNYY200 pKa = 9.77 SEE202 pKa = 4.25 IRR204 pKa = 11.84 QTGDD208 pKa = 2.37 WNTADD213 pKa = 4.7 LLQDD217 pKa = 3.67 GADD220 pKa = 3.29 ISLTAVNKK228 pKa = 10.43 DD229 pKa = 3.62 EE230 pKa = 4.49 SYY232 pKa = 9.05 SWQIGVHH239 pKa = 4.61 STLNVKK245 pKa = 9.73 QVGDD249 pKa = 3.52 VDD251 pKa = 3.83 EE252 pKa = 4.49 QLAEE256 pKa = 4.14 SRR258 pKa = 11.84 QNGSGNTTNILQVGVTMQNVDD279 pKa = 2.6 AWQYY283 pKa = 8.08 GTDD286 pKa = 4.08 VIDD289 pKa = 3.66 IDD291 pKa = 4.02 QVDD294 pKa = 4.14 VEE296 pKa = 4.46 PEE298 pKa = 3.79 EE299 pKa = 4.1
Molecular weight: 32.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.554
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.91
Patrickios 1.189
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|X5DLY7|X5DLY7_9BACT Curlin associated repeat-containing protein OS=Draconibacterium orientale OX=1168034 GN=FH5T_18445 PE=3 SV=1
MM1 pKa = 7.6 LRR3 pKa = 11.84 TDD5 pKa = 3.53 CRR7 pKa = 11.84 KK8 pKa = 10.16 LKK10 pKa = 10.65 NSNQRR15 pKa = 11.84 KK16 pKa = 8.04 QLILNLPQVPEE27 pKa = 3.98 VSFKK31 pKa = 10.88 RR32 pKa = 11.84 VFLRR36 pKa = 11.84 PQLTEE41 pKa = 3.66 NGFKK45 pKa = 10.33 RR46 pKa = 11.84 VSLLHH51 pKa = 5.64 QVTEE55 pKa = 4.34 EE56 pKa = 4.0 GFKK59 pKa = 10.68 RR60 pKa = 11.84 IFARR64 pKa = 11.84 RR65 pKa = 11.84 QINRR69 pKa = 11.84 VDD71 pKa = 3.36 TKK73 pKa = 10.94 RR74 pKa = 11.84 IFSCAQHH81 pKa = 6.19 IAGSTKK87 pKa = 10.3 HH88 pKa = 6.51 ILTGHH93 pKa = 5.61 CHH95 pKa = 5.64 MGIKK99 pKa = 10.19 NISFYY104 pKa = 11.34 SVVIQKK110 pKa = 8.54 TGNSHH115 pKa = 6.82 VEE117 pKa = 3.32 ICYY120 pKa = 10.21 LRR122 pKa = 11.84 SGPFFTLAYY131 pKa = 10.27 FMAKK135 pKa = 9.74 NVV137 pKa = 3.29
Molecular weight: 15.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.706
IPC_protein 10.204
Toseland 10.789
ProMoST 10.365
Dawson 10.862
Bjellqvist 10.511
Wikipedia 11.023
Rodwell 11.242
Grimsley 10.891
Solomon 10.95
Lehninger 10.921
Nozaki 10.774
DTASelect 10.511
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.979
IPC_peptide 10.95
IPC2_peptide 9.545
IPC2.peptide.svr19 8.591
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3940
0
3940
1407688
48
3688
357.3
40.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.891 ± 0.034
0.844 ± 0.016
5.585 ± 0.033
7.037 ± 0.037
5.064 ± 0.028
6.758 ± 0.04
1.806 ± 0.015
7.275 ± 0.039
7.035 ± 0.047
9.135 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.411 ± 0.018
5.702 ± 0.031
3.662 ± 0.023
3.504 ± 0.025
3.872 ± 0.024
6.091 ± 0.028
5.438 ± 0.04
6.544 ± 0.028
1.271 ± 0.016
4.074 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here