Nocardioides luteus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5357 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J4N5J0|A0A1J4N5J0_9ACTN Amidohydrolase OS=Nocardioides luteus OX=1844 GN=UG56_010660 PE=4 SV=1
MM1 pKa = 7.71ILTGYY6 pKa = 10.19DD7 pKa = 3.08VYY9 pKa = 10.54EE10 pKa = 4.36TYY12 pKa = 11.34GLAGDD17 pKa = 3.93DD18 pKa = 4.03TICRR22 pKa = 11.84VEE24 pKa = 4.39DD25 pKa = 4.01GPRR28 pKa = 11.84GKK30 pKa = 10.01IYY32 pKa = 10.73GGDD35 pKa = 3.84GDD37 pKa = 4.25DD38 pKa = 4.03VIYY41 pKa = 10.75GGIVEE46 pKa = 4.16YY47 pKa = 10.46SGPEE51 pKa = 3.55IYY53 pKa = 10.78GGDD56 pKa = 4.01GNDD59 pKa = 3.85TIYY62 pKa = 11.08GSNGWTYY69 pKa = 11.3INAGDD74 pKa = 4.07GADD77 pKa = 3.4TVYY80 pKa = 10.75VRR82 pKa = 11.84ARR84 pKa = 11.84GGDD87 pKa = 3.55VYY89 pKa = 11.32GGAGNDD95 pKa = 3.61LVYY98 pKa = 11.06GGAGNDD104 pKa = 3.67AMWAGSGNDD113 pKa = 3.03RR114 pKa = 11.84VYY116 pKa = 11.35GGLGDD121 pKa = 4.38DD122 pKa = 4.25FLVGGSDD129 pKa = 2.97HH130 pKa = 7.48DD131 pKa = 4.1YY132 pKa = 11.41VYY134 pKa = 11.26GGDD137 pKa = 4.22GNDD140 pKa = 3.79LMTGLGGDD148 pKa = 3.5DD149 pKa = 3.63RR150 pKa = 11.84LYY152 pKa = 11.31GGAGADD158 pKa = 3.87QINGADD164 pKa = 3.67GNDD167 pKa = 4.24RR168 pKa = 11.84IWGSSGGDD176 pKa = 3.41TINAGGGADD185 pKa = 4.3RR186 pKa = 11.84IWGEE190 pKa = 3.89GGNDD194 pKa = 3.06EE195 pKa = 4.55VEE197 pKa = 4.4AGGGADD203 pKa = 3.63QVFGGAGWDD212 pKa = 3.54WLSGGSGNDD221 pKa = 3.07ILSGGDD227 pKa = 3.18GRR229 pKa = 11.84DD230 pKa = 3.3RR231 pKa = 11.84LIGGSGTDD239 pKa = 3.53KK240 pKa = 11.22LSGGSGADD248 pKa = 3.03RR249 pKa = 11.84LTYY252 pKa = 10.76

Molecular weight: 25.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J4N109|A0A1J4N109_9ACTN Single-stranded DNA-binding protein OS=Nocardioides luteus OX=1844 GN=UG56_018405 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.64KK15 pKa = 8.79THH17 pKa = 6.48GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.67GRR40 pKa = 11.84ASLTVV45 pKa = 3.04

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5357

0

5357

1728418

32

2075

322.6

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.879 ± 0.05

0.65 ± 0.01

6.28 ± 0.027

6.089 ± 0.034

2.906 ± 0.019

9.118 ± 0.03

2.131 ± 0.018

4.066 ± 0.023

2.366 ± 0.026

10.127 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.902 ± 0.013

1.916 ± 0.018

5.486 ± 0.028

2.682 ± 0.016

7.032 ± 0.04

5.387 ± 0.026

6.26 ± 0.031

9.009 ± 0.03

1.569 ± 0.012

2.147 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski