Alsobacter soli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Alsobacteraceae; Alsobacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4752 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2T1HX16|A0A2T1HX16_9RHIZ Lytic transglycosylase OS=Alsobacter soli OX=2109933 GN=SLNSH_04955 PE=4 SV=1
MM1 pKa = 7.95GDD3 pKa = 3.71TLSGGAGDD11 pKa = 4.13DD12 pKa = 4.46LIYY15 pKa = 10.94GSGGADD21 pKa = 3.31SLDD24 pKa = 3.58GGAGNDD30 pKa = 3.6TLVAGSGADD39 pKa = 3.54TLVGGAGVDD48 pKa = 3.48TADD51 pKa = 3.48YY52 pKa = 11.02SAFPTLISNGDD63 pKa = 3.59FSSAATGWTLGSNTAVTSGAAQATNATRR91 pKa = 11.84MLTQDD96 pKa = 2.81VGFVVGQTYY105 pKa = 7.93TVTFDD110 pKa = 3.39YY111 pKa = 11.19TMTAGARR118 pKa = 11.84LRR120 pKa = 11.84ISNSDD125 pKa = 3.32TNGANVVYY133 pKa = 9.72TSTVLDD139 pKa = 3.53SAGTVTATFVATGSKK154 pKa = 10.33LGIEE158 pKa = 4.39ADD160 pKa = 3.53SGSFTGTLDD169 pKa = 4.1NIRR172 pKa = 11.84MLQVVDD178 pKa = 4.29LATGKK183 pKa = 11.07SNDD186 pKa = 3.25GDD188 pKa = 3.67VLQGVEE194 pKa = 4.19NVIGGATGNKK204 pKa = 9.43IYY206 pKa = 10.86GDD208 pKa = 3.6TGANRR213 pKa = 11.84LDD215 pKa = 3.75GGAGNDD221 pKa = 3.65TLAGGSGADD230 pKa = 3.73TLAGGAGNDD239 pKa = 3.36RR240 pKa = 11.84LNGGEE245 pKa = 4.1AWLADD250 pKa = 4.06KK251 pKa = 10.92EE252 pKa = 4.36PASLTNAYY260 pKa = 10.09LFQNKK265 pKa = 7.57ATSFYY270 pKa = 10.59NGEE273 pKa = 4.17SLYY276 pKa = 10.56IPAQYY281 pKa = 8.73THH283 pKa = 7.05NIFEE287 pKa = 4.93HH288 pKa = 6.48PNSGAGEE295 pKa = 4.2TVAVYY300 pKa = 10.86NLGGATTFSSDD311 pKa = 4.52FVTNWSTSDD320 pKa = 3.13GATFRR325 pKa = 11.84VYY327 pKa = 11.34ADD329 pKa = 3.27GALIFEE335 pKa = 4.87KK336 pKa = 10.68VDD338 pKa = 3.48APSSQVFSTGLLNVAGASQLKK359 pKa = 10.18LVTTAGTSGNNGADD373 pKa = 2.93HH374 pKa = 7.11AMWLNPRR381 pKa = 11.84LSWGIADD388 pKa = 4.22GADD391 pKa = 4.03SISGGAGDD399 pKa = 4.3DD400 pKa = 3.97TIEE403 pKa = 4.48GGFGADD409 pKa = 3.51TLDD412 pKa = 3.89GGSGVDD418 pKa = 3.42TLDD421 pKa = 3.41YY422 pKa = 9.41TASTGAVTVNLATGAASGGDD442 pKa = 3.5AQGDD446 pKa = 3.76IISGFEE452 pKa = 4.14NVWGSAQADD461 pKa = 4.14SLVGDD466 pKa = 4.3AGANIFIGGLGADD479 pKa = 4.37TIVGGAGVDD488 pKa = 3.5TVDD491 pKa = 4.0YY492 pKa = 8.65STSGMNPAGGVGFFVDD508 pKa = 4.07LLAGKK513 pKa = 8.79GHH515 pKa = 6.63NGAAEE520 pKa = 4.23GDD522 pKa = 3.95VLSGIEE528 pKa = 3.99NLVGSVWNDD537 pKa = 3.06KK538 pKa = 11.08LYY540 pKa = 11.44GDD542 pKa = 4.77AGNNRR547 pKa = 11.84IDD549 pKa = 3.6GGGWGNDD556 pKa = 3.66LLSGGAGNDD565 pKa = 3.66TLIGGAGNEE574 pKa = 3.99RR575 pKa = 11.84LVGGLTWLADD585 pKa = 3.58IAPTSQTNILQYY597 pKa = 9.41STPGPGGLIPFQYY610 pKa = 9.48QHH612 pKa = 6.91NIFEE616 pKa = 5.1HH617 pKa = 6.3PNSTGEE623 pKa = 3.99AVLTYY628 pKa = 10.17DD629 pKa = 3.9IGSATTFSASFIKK642 pKa = 10.6NVSAGGDD649 pKa = 3.47GVTWRR654 pKa = 11.84VYY656 pKa = 11.2GDD658 pKa = 3.39STLLFEE664 pKa = 5.27MADD667 pKa = 3.46APLGQMFSTGVINVAGYY684 pKa = 8.91QQLKK688 pKa = 10.44LVTTSGSAGNNGFDD702 pKa = 3.24SAYY705 pKa = 9.02WLNPVLSSGAVGSDD719 pKa = 2.8NDD721 pKa = 4.36SLVGGAGNDD730 pKa = 3.72TLEE733 pKa = 4.68GGAGADD739 pKa = 3.86TMDD742 pKa = 4.38GGAGTDD748 pKa = 3.49TLDD751 pKa = 3.59YY752 pKa = 9.81AASSAAVTVDD762 pKa = 3.72LSANTASGGDD772 pKa = 3.48AQGDD776 pKa = 4.02VISGFEE782 pKa = 4.09YY783 pKa = 10.83VLGSAFADD791 pKa = 5.12RR792 pKa = 11.84ITGDD796 pKa = 3.92ANANWFYY803 pKa = 11.18GGSGDD808 pKa = 4.39DD809 pKa = 3.87TLIGAAGADD818 pKa = 3.58TMVGGWGADD827 pKa = 3.36SMDD830 pKa = 3.99GGSGVDD836 pKa = 3.27TLDD839 pKa = 3.44YY840 pKa = 9.82TGSSTGVTVNLATGVGLGGDD860 pKa = 3.69AQGDD864 pKa = 3.91TLIGFEE870 pKa = 4.49NVWGTSSADD879 pKa = 3.63SLVGDD884 pKa = 4.19ANDD887 pKa = 3.92NIFRR891 pKa = 11.84PYY893 pKa = 10.52LGADD897 pKa = 3.94TIVGGAGQDD906 pKa = 3.59TVDD909 pKa = 3.17YY910 pKa = 8.78WYY912 pKa = 10.88SGTGVSIDD920 pKa = 3.93LTLSTAQSGGDD931 pKa = 3.21AAGDD935 pKa = 3.46VFSGIEE941 pKa = 3.94NVIGSTTAANWIKK954 pKa = 11.15GDD956 pKa = 3.77ANANALTGWNGNDD969 pKa = 3.36TLIGGGGADD978 pKa = 3.9TLSGGAGNDD987 pKa = 3.07AFTLDD992 pKa = 3.94GSSLTIAAKK1001 pKa = 10.49VDD1003 pKa = 3.3GGTGADD1009 pKa = 3.55TLTIAANSGSSFTAANLAAAVTNMEE1034 pKa = 4.47VIDD1037 pKa = 3.87FRR1039 pKa = 11.84QAGVNANLSLSGAQVAAMTDD1059 pKa = 3.31ANKK1062 pKa = 10.76DD1063 pKa = 3.42LTINTSLGGGDD1074 pKa = 4.15TIAIADD1080 pKa = 3.67AASRR1084 pKa = 11.84YY1085 pKa = 7.39TMSVSGNTTNYY1096 pKa = 10.66DD1097 pKa = 3.02IYY1099 pKa = 11.35ADD1101 pKa = 4.16DD1102 pKa = 3.7AHH1104 pKa = 5.89TTLLAHH1110 pKa = 6.29LHH1112 pKa = 5.27VVAAA1116 pKa = 4.72

Molecular weight:
110.57 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2T1HRP6|A0A2T1HRP6_9RHIZ 30S ribosomal protein S4 OS=Alsobacter soli OX=2109933 GN=rpsD PE=3 SV=1
AA1 pKa = 7.47GRR3 pKa = 11.84GGGGGAARR11 pKa = 11.84SGWTGLATGSGRR23 pKa = 11.84AGRR26 pKa = 11.84AGSGRR31 pKa = 11.84ASARR35 pKa = 11.84STGSGRR41 pKa = 11.84IAGSGRR47 pKa = 11.84RR48 pKa = 11.84TGSARR53 pKa = 11.84RR54 pKa = 11.84SGSGRR59 pKa = 11.84ITSGRR64 pKa = 11.84RR65 pKa = 11.84GGGGGGLRR73 pKa = 4.03

Molecular weight:
6.71 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4752

0

4752

1490446

30

2818

313.6

33.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.787 ± 0.051

0.839 ± 0.01

5.387 ± 0.031

5.6 ± 0.034

3.559 ± 0.022

8.931 ± 0.038

1.884 ± 0.018

4.421 ± 0.028

3.042 ± 0.031

10.246 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.016

2.221 ± 0.02

5.635 ± 0.027

3.067 ± 0.022

7.669 ± 0.04

5.154 ± 0.025

4.898 ± 0.023

7.897 ± 0.036

1.343 ± 0.015

2.065 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski