Alsobacter soli
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4752 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T1HX16|A0A2T1HX16_9RHIZ Lytic transglycosylase OS=Alsobacter soli OX=2109933 GN=SLNSH_04955 PE=4 SV=1
MM1 pKa = 7.95 GDD3 pKa = 3.71 TLSGGAGDD11 pKa = 4.13 DD12 pKa = 4.46 LIYY15 pKa = 10.94 GSGGADD21 pKa = 3.31 SLDD24 pKa = 3.58 GGAGNDD30 pKa = 3.6 TLVAGSGADD39 pKa = 3.54 TLVGGAGVDD48 pKa = 3.48 TADD51 pKa = 3.48 YY52 pKa = 11.02 SAFPTLISNGDD63 pKa = 3.59 FSSAATGWTLGSNTAVTSGAAQATNATRR91 pKa = 11.84 MLTQDD96 pKa = 2.81 VGFVVGQTYY105 pKa = 7.93 TVTFDD110 pKa = 3.39 YY111 pKa = 11.19 TMTAGARR118 pKa = 11.84 LRR120 pKa = 11.84 ISNSDD125 pKa = 3.32 TNGANVVYY133 pKa = 9.72 TSTVLDD139 pKa = 3.53 SAGTVTATFVATGSKK154 pKa = 10.33 LGIEE158 pKa = 4.39 ADD160 pKa = 3.53 SGSFTGTLDD169 pKa = 4.1 NIRR172 pKa = 11.84 MLQVVDD178 pKa = 4.29 LATGKK183 pKa = 11.07 SNDD186 pKa = 3.25 GDD188 pKa = 3.67 VLQGVEE194 pKa = 4.19 NVIGGATGNKK204 pKa = 9.43 IYY206 pKa = 10.86 GDD208 pKa = 3.6 TGANRR213 pKa = 11.84 LDD215 pKa = 3.75 GGAGNDD221 pKa = 3.65 TLAGGSGADD230 pKa = 3.73 TLAGGAGNDD239 pKa = 3.36 RR240 pKa = 11.84 LNGGEE245 pKa = 4.1 AWLADD250 pKa = 4.06 KK251 pKa = 10.92 EE252 pKa = 4.36 PASLTNAYY260 pKa = 10.09 LFQNKK265 pKa = 7.57 ATSFYY270 pKa = 10.59 NGEE273 pKa = 4.17 SLYY276 pKa = 10.56 IPAQYY281 pKa = 8.73 THH283 pKa = 7.05 NIFEE287 pKa = 4.93 HH288 pKa = 6.48 PNSGAGEE295 pKa = 4.2 TVAVYY300 pKa = 10.86 NLGGATTFSSDD311 pKa = 4.52 FVTNWSTSDD320 pKa = 3.13 GATFRR325 pKa = 11.84 VYY327 pKa = 11.34 ADD329 pKa = 3.27 GALIFEE335 pKa = 4.87 KK336 pKa = 10.68 VDD338 pKa = 3.48 APSSQVFSTGLLNVAGASQLKK359 pKa = 10.18 LVTTAGTSGNNGADD373 pKa = 2.93 HH374 pKa = 7.11 AMWLNPRR381 pKa = 11.84 LSWGIADD388 pKa = 4.22 GADD391 pKa = 4.03 SISGGAGDD399 pKa = 4.3 DD400 pKa = 3.97 TIEE403 pKa = 4.48 GGFGADD409 pKa = 3.51 TLDD412 pKa = 3.89 GGSGVDD418 pKa = 3.42 TLDD421 pKa = 3.41 YY422 pKa = 9.41 TASTGAVTVNLATGAASGGDD442 pKa = 3.5 AQGDD446 pKa = 3.76 IISGFEE452 pKa = 4.14 NVWGSAQADD461 pKa = 4.14 SLVGDD466 pKa = 4.3 AGANIFIGGLGADD479 pKa = 4.37 TIVGGAGVDD488 pKa = 3.5 TVDD491 pKa = 4.0 YY492 pKa = 8.65 STSGMNPAGGVGFFVDD508 pKa = 4.07 LLAGKK513 pKa = 8.79 GHH515 pKa = 6.63 NGAAEE520 pKa = 4.23 GDD522 pKa = 3.95 VLSGIEE528 pKa = 3.99 NLVGSVWNDD537 pKa = 3.06 KK538 pKa = 11.08 LYY540 pKa = 11.44 GDD542 pKa = 4.77 AGNNRR547 pKa = 11.84 IDD549 pKa = 3.6 GGGWGNDD556 pKa = 3.66 LLSGGAGNDD565 pKa = 3.66 TLIGGAGNEE574 pKa = 3.99 RR575 pKa = 11.84 LVGGLTWLADD585 pKa = 3.58 IAPTSQTNILQYY597 pKa = 9.41 STPGPGGLIPFQYY610 pKa = 9.48 QHH612 pKa = 6.91 NIFEE616 pKa = 5.1 HH617 pKa = 6.3 PNSTGEE623 pKa = 3.99 AVLTYY628 pKa = 10.17 DD629 pKa = 3.9 IGSATTFSASFIKK642 pKa = 10.6 NVSAGGDD649 pKa = 3.47 GVTWRR654 pKa = 11.84 VYY656 pKa = 11.2 GDD658 pKa = 3.39 STLLFEE664 pKa = 5.27 MADD667 pKa = 3.46 APLGQMFSTGVINVAGYY684 pKa = 8.91 QQLKK688 pKa = 10.44 LVTTSGSAGNNGFDD702 pKa = 3.24 SAYY705 pKa = 9.02 WLNPVLSSGAVGSDD719 pKa = 2.8 NDD721 pKa = 4.36 SLVGGAGNDD730 pKa = 3.72 TLEE733 pKa = 4.68 GGAGADD739 pKa = 3.86 TMDD742 pKa = 4.38 GGAGTDD748 pKa = 3.49 TLDD751 pKa = 3.59 YY752 pKa = 9.81 AASSAAVTVDD762 pKa = 3.72 LSANTASGGDD772 pKa = 3.48 AQGDD776 pKa = 4.02 VISGFEE782 pKa = 4.09 YY783 pKa = 10.83 VLGSAFADD791 pKa = 5.12 RR792 pKa = 11.84 ITGDD796 pKa = 3.92 ANANWFYY803 pKa = 11.18 GGSGDD808 pKa = 4.39 DD809 pKa = 3.87 TLIGAAGADD818 pKa = 3.58 TMVGGWGADD827 pKa = 3.36 SMDD830 pKa = 3.99 GGSGVDD836 pKa = 3.27 TLDD839 pKa = 3.44 YY840 pKa = 9.82 TGSSTGVTVNLATGVGLGGDD860 pKa = 3.69 AQGDD864 pKa = 3.91 TLIGFEE870 pKa = 4.49 NVWGTSSADD879 pKa = 3.63 SLVGDD884 pKa = 4.19 ANDD887 pKa = 3.92 NIFRR891 pKa = 11.84 PYY893 pKa = 10.52 LGADD897 pKa = 3.94 TIVGGAGQDD906 pKa = 3.59 TVDD909 pKa = 3.17 YY910 pKa = 8.78 WYY912 pKa = 10.88 SGTGVSIDD920 pKa = 3.93 LTLSTAQSGGDD931 pKa = 3.21 AAGDD935 pKa = 3.46 VFSGIEE941 pKa = 3.94 NVIGSTTAANWIKK954 pKa = 11.15 GDD956 pKa = 3.77 ANANALTGWNGNDD969 pKa = 3.36 TLIGGGGADD978 pKa = 3.9 TLSGGAGNDD987 pKa = 3.07 AFTLDD992 pKa = 3.94 GSSLTIAAKK1001 pKa = 10.49 VDD1003 pKa = 3.3 GGTGADD1009 pKa = 3.55 TLTIAANSGSSFTAANLAAAVTNMEE1034 pKa = 4.47 VIDD1037 pKa = 3.87 FRR1039 pKa = 11.84 QAGVNANLSLSGAQVAAMTDD1059 pKa = 3.31 ANKK1062 pKa = 10.76 DD1063 pKa = 3.42 LTINTSLGGGDD1074 pKa = 4.15 TIAIADD1080 pKa = 3.67 AASRR1084 pKa = 11.84 YY1085 pKa = 7.39 TMSVSGNTTNYY1096 pKa = 10.66 DD1097 pKa = 3.02 IYY1099 pKa = 11.35 ADD1101 pKa = 4.16 DD1102 pKa = 3.7 AHH1104 pKa = 5.89 TTLLAHH1110 pKa = 6.29 LHH1112 pKa = 5.27 VVAAA1116 pKa = 4.72
Molecular weight: 110.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.516
IPC_protein 3.592
Toseland 3.338
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.757
Wikipedia 3.579
Rodwell 3.401
Grimsley 3.236
Solomon 3.605
Lehninger 3.567
Nozaki 3.706
DTASelect 4.05
Thurlkill 3.401
EMBOSS 3.579
Sillero 3.719
Patrickios 1.494
IPC_peptide 3.592
IPC2_peptide 3.694
IPC2.peptide.svr19 3.689
Protein with the highest isoelectric point:
>tr|A0A2T1HRP6|A0A2T1HRP6_9RHIZ 30S ribosomal protein S4 OS=Alsobacter soli OX=2109933 GN=rpsD PE=3 SV=1
AA1 pKa = 7.47 GRR3 pKa = 11.84 GGGGGAARR11 pKa = 11.84 SGWTGLATGSGRR23 pKa = 11.84 AGRR26 pKa = 11.84 AGSGRR31 pKa = 11.84 ASARR35 pKa = 11.84 STGSGRR41 pKa = 11.84 IAGSGRR47 pKa = 11.84 RR48 pKa = 11.84 TGSARR53 pKa = 11.84 RR54 pKa = 11.84 SGSGRR59 pKa = 11.84 ITSGRR64 pKa = 11.84 RR65 pKa = 11.84 GGGGGGLRR73 pKa = 4.03
Molecular weight: 6.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.524
IPC2_protein 11.374
IPC_protein 12.983
Toseland 13.144
ProMoST 13.656
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.647
Grimsley 13.188
Solomon 13.642
Lehninger 13.539
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.369
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.303
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4752
0
4752
1490446
30
2818
313.6
33.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.787 ± 0.051
0.839 ± 0.01
5.387 ± 0.031
5.6 ± 0.034
3.559 ± 0.022
8.931 ± 0.038
1.884 ± 0.018
4.421 ± 0.028
3.042 ± 0.031
10.246 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.016
2.221 ± 0.02
5.635 ± 0.027
3.067 ± 0.022
7.669 ± 0.04
5.154 ± 0.025
4.898 ± 0.023
7.897 ± 0.036
1.343 ± 0.015
2.065 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here