Breoghania sp. L-A4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Breoghaniaceae; Breoghania; unclassified Breoghania

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4084 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346R4X8|A0A346R4X8_9RHIZ GntR family transcriptional regulator OS=Breoghania sp. L-A4 OX=2304600 GN=D1F64_17895 PE=4 SV=1
MM1 pKa = 7.6SGTGNTTKK9 pKa = 10.59SVASTGNQFIDD20 pKa = 4.17GVLTYY25 pKa = 10.33DD26 pKa = 3.23AWADD30 pKa = 3.31STLYY34 pKa = 10.94YY35 pKa = 10.78SFSTSSAAYY44 pKa = 9.93SYY46 pKa = 10.68TGTGNEE52 pKa = 4.3DD53 pKa = 3.05LPANFNAITAAQQTAAHH70 pKa = 6.59FALSADD76 pKa = 4.49DD77 pKa = 5.14GPSASAGFSLEE88 pKa = 3.68GFTAINVASLGSTNSSTAEE107 pKa = 3.45IRR109 pKa = 11.84FGEE112 pKa = 4.43TSSSSLGTAQVADD125 pKa = 4.27FPGNYY130 pKa = 7.48ITGPSGDD137 pKa = 4.4NGDD140 pKa = 3.46VWFGSYY146 pKa = 9.84SGYY149 pKa = 10.42IYY151 pKa = 8.17RR152 pKa = 11.84TPVAGNYY159 pKa = 8.57AWHH162 pKa = 6.05THH164 pKa = 4.24LHH166 pKa = 6.28EE167 pKa = 5.26IGHH170 pKa = 6.46ALGLKK175 pKa = 9.87HH176 pKa = 6.06GQEE179 pKa = 4.22NSGAGPTPAAYY190 pKa = 10.21DD191 pKa = 3.51SMEE194 pKa = 4.2YY195 pKa = 10.89SVMTYY200 pKa = 9.8RR201 pKa = 11.84SYY203 pKa = 12.09VNDD206 pKa = 4.26PLIGGYY212 pKa = 9.39SNEE215 pKa = 4.35TYY217 pKa = 10.61GYY219 pKa = 8.22AQSYY223 pKa = 8.49MMADD227 pKa = 3.49IAALQHH233 pKa = 6.14MYY235 pKa = 10.79GADD238 pKa = 3.36FTTNSGDD245 pKa = 3.86TVYY248 pKa = 10.64KK249 pKa = 10.01WNPNSGDD256 pKa = 3.6TLVNGAVAIDD266 pKa = 3.47AGGNRR271 pKa = 11.84IFATVWDD278 pKa = 4.19GGGTDD283 pKa = 3.72TYY285 pKa = 11.43DD286 pKa = 3.09LSSYY290 pKa = 7.54TTALMLDD297 pKa = 4.3LRR299 pKa = 11.84PGEE302 pKa = 4.25HH303 pKa = 6.36SVFSAVQLAYY313 pKa = 10.78LGDD316 pKa = 3.88GNYY319 pKa = 10.31SRR321 pKa = 11.84GNIFNALQYY330 pKa = 10.93QSDD333 pKa = 3.79TRR335 pKa = 11.84SLIEE339 pKa = 3.81NAIGGSGDD347 pKa = 3.52DD348 pKa = 4.24TITGNAANNTLQGGNGADD366 pKa = 3.45MLYY369 pKa = 10.97GGLGNDD375 pKa = 3.72TLEE378 pKa = 4.69GGAGADD384 pKa = 4.01LLDD387 pKa = 4.56GQGGWDD393 pKa = 3.33MASYY397 pKa = 10.13AGSSAAVTVNLGDD410 pKa = 3.94GLTEE414 pKa = 3.98TGGDD418 pKa = 3.56AEE420 pKa = 4.61GDD422 pKa = 3.24QLLRR426 pKa = 11.84IEE428 pKa = 4.5HH429 pKa = 6.56LEE431 pKa = 3.9GSVFHH436 pKa = 7.18DD437 pKa = 3.57TLIGNAGINYY447 pKa = 9.84LYY449 pKa = 10.62GGNGNDD455 pKa = 3.72TLEE458 pKa = 4.62GGAGADD464 pKa = 4.01LLDD467 pKa = 4.7GQGGWDD473 pKa = 3.23RR474 pKa = 11.84ASYY477 pKa = 10.1AGSSAAVTVNLGDD490 pKa = 3.94GLTEE494 pKa = 3.98TGGDD498 pKa = 3.59AEE500 pKa = 4.83GDD502 pKa = 3.38LLLRR506 pKa = 11.84IEE508 pKa = 4.57HH509 pKa = 6.75LEE511 pKa = 4.15GSAFDD516 pKa = 3.78DD517 pKa = 3.94TLIGSAGINYY527 pKa = 10.04LYY529 pKa = 10.98GGDD532 pKa = 4.25GNDD535 pKa = 3.66TLEE538 pKa = 4.65GGAGADD544 pKa = 3.65RR545 pKa = 11.84LDD547 pKa = 4.27GQGGWDD553 pKa = 3.18RR554 pKa = 11.84ASYY557 pKa = 9.01TGSSAAVTVNLGDD570 pKa = 3.85GLVEE574 pKa = 4.18SGGDD578 pKa = 3.49AEE580 pKa = 5.25GDD582 pKa = 3.18QLLRR586 pKa = 11.84IEE588 pKa = 4.66HH589 pKa = 6.58LEE591 pKa = 4.04GSAFHH596 pKa = 6.97DD597 pKa = 3.86TLIGSAGINYY607 pKa = 10.04LYY609 pKa = 10.98GGDD612 pKa = 4.25GNDD615 pKa = 3.66TLEE618 pKa = 4.65GGAGADD624 pKa = 3.65RR625 pKa = 11.84LDD627 pKa = 4.21GQGGWDD633 pKa = 3.33MASYY637 pKa = 10.13AGSSAAVSVNLGDD650 pKa = 4.12GLVEE654 pKa = 4.18SGGDD658 pKa = 3.5AEE660 pKa = 4.7GDD662 pKa = 3.06RR663 pKa = 11.84LLRR666 pKa = 11.84IEE668 pKa = 4.6HH669 pKa = 6.72LEE671 pKa = 4.15GSAFSDD677 pKa = 3.59TLIGSAGINYY687 pKa = 9.51LFGGDD692 pKa = 4.18GNDD695 pKa = 3.65TLEE698 pKa = 4.65GGAGADD704 pKa = 3.65RR705 pKa = 11.84LDD707 pKa = 4.52GQGGWDD713 pKa = 3.21KK714 pKa = 11.25VSYY717 pKa = 9.8AGSSAGVSVNLGDD730 pKa = 4.24GLVEE734 pKa = 4.18SGGDD738 pKa = 3.49AEE740 pKa = 5.25GDD742 pKa = 3.18QLLRR746 pKa = 11.84IEE748 pKa = 4.42HH749 pKa = 6.8LEE751 pKa = 4.17GSSYY755 pKa = 11.72ADD757 pKa = 3.27ILTGSAGINWMGGGAGADD775 pKa = 3.58EE776 pKa = 4.3LAGGGGNDD784 pKa = 3.62RR785 pKa = 11.84LSGGTGADD793 pKa = 3.03DD794 pKa = 5.38FIFNTGDD801 pKa = 3.37GSDD804 pKa = 3.89TITDD808 pKa = 4.39FEE810 pKa = 5.85LGLDD814 pKa = 4.5DD815 pKa = 5.64IRR817 pKa = 11.84ILSGASSFGDD827 pKa = 3.41LTITDD832 pKa = 3.8SGANALVQFADD843 pKa = 3.99VSITLLNVDD852 pKa = 4.21HH853 pKa = 6.95TLLMSDD859 pKa = 4.73DD860 pKa = 4.06FLFAA864 pKa = 5.48

Molecular weight: 87.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346QXF9|A0A346QXF9_9RHIZ Heme exporter protein D OS=Breoghania sp. L-A4 OX=2304600 GN=ccmD PE=3 SV=1
MM1 pKa = 6.76WTRR4 pKa = 11.84IFISVLVFMQINAVLFGAGIVAVLVVPALKK34 pKa = 10.19AQAMMLLPAVVVASLVIALPVSWWLAPRR62 pKa = 11.84LRR64 pKa = 11.84LPRR67 pKa = 11.84WRR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 5.23AANAGQQ79 pKa = 3.38

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4084

0

4084

1258513

33

2978

308.2

33.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.67 ± 0.052

0.922 ± 0.013

6.034 ± 0.031

5.731 ± 0.033

3.726 ± 0.024

8.624 ± 0.038

2.049 ± 0.017

5.303 ± 0.029

3.123 ± 0.028

9.897 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.02

2.544 ± 0.019

5.057 ± 0.028

2.904 ± 0.024

7.075 ± 0.041

5.442 ± 0.028

5.47 ± 0.031

7.39 ± 0.035

1.284 ± 0.013

2.185 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski