Klebsiella virus 2019KP1
Average proteome isoelectric point is 7.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M5CAN4|A0A6M5CAN4_9CAUD Uncharacterized protein OS=Klebsiella virus 2019KP1 OX=2736194 GN=KP1_21 PE=4 SV=1
MM1 pKa = 8.07 RR2 pKa = 11.84 IMDD5 pKa = 4.31 KK6 pKa = 10.95 VLNAYY11 pKa = 8.83 KK12 pKa = 10.55 KK13 pKa = 10.49 LVLAVSSVTHH23 pKa = 6.85 DD24 pKa = 3.32 AASGFDD30 pKa = 4.12 RR31 pKa = 11.84 GDD33 pKa = 3.3 LSDD36 pKa = 5.3 VYY38 pKa = 11.29 DD39 pKa = 4.81 ALDD42 pKa = 3.99 KK43 pKa = 11.12 LAALYY48 pKa = 10.99 GMDD51 pKa = 4.95 LEE53 pKa = 4.79 LAATAFKK60 pKa = 10.34 EE61 pKa = 4.22 HH62 pKa = 6.95 NDD64 pKa = 3.79 LAVHH68 pKa = 6.52 ADD70 pKa = 3.46 KK71 pKa = 11.3 LRR73 pKa = 11.84 GDD75 pKa = 4.17 DD76 pKa = 3.65 LVLIRR81 pKa = 11.84 VVGTLSIGLAEE92 pKa = 4.81 IGSCIYY98 pKa = 10.86 DD99 pKa = 3.38 ADD101 pKa = 3.65 QSLRR105 pKa = 11.84 TPEE108 pKa = 4.5 VIGDD112 pKa = 3.64 MLGTVLVLSEE122 pKa = 4.68 LEE124 pKa = 4.0 AA125 pKa = 5.95
Molecular weight: 13.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.456
IPC2_protein 4.38
IPC_protein 4.329
Toseland 4.139
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.266
Rodwell 4.164
Grimsley 4.05
Solomon 4.317
Lehninger 4.279
Nozaki 4.444
DTASelect 4.698
Thurlkill 4.177
EMBOSS 4.279
Sillero 4.457
Patrickios 3.872
IPC_peptide 4.317
IPC2_peptide 4.444
IPC2.peptide.svr19 4.381
Protein with the highest isoelectric point:
>tr|A0A6M5CCK9|A0A6M5CCK9_9CAUD Putative DNA helicase OS=Klebsiella virus 2019KP1 OX=2736194 GN=KP1_52 PE=4 SV=1
MM1 pKa = 7.75 AARR4 pKa = 11.84 TVLDD8 pKa = 3.87 AVNLGIITDD17 pKa = 3.95 WNNSMVNVFNIIVTSAMLVLGNDD40 pKa = 3.38 ASNPIPYY47 pKa = 8.66 CTVQLQQPATQQPQPRR63 pKa = 11.84 LSMTSLKK70 pKa = 10.07 TLMVVAKK77 pKa = 9.65 NWAHH81 pKa = 5.82 VYY83 pKa = 10.2 LRR85 pKa = 11.84 RR86 pKa = 11.84 CRR88 pKa = 11.84 SSTGRR93 pKa = 11.84 RR94 pKa = 11.84 GDD96 pKa = 3.42 AHH98 pKa = 7.24 CGRR101 pKa = 4.94
Molecular weight: 11.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.355
IPC_protein 9.97
Toseland 10.014
ProMoST 10.262
Dawson 10.248
Bjellqvist 10.058
Wikipedia 10.482
Rodwell 10.379
Grimsley 10.335
Solomon 10.35
Lehninger 10.321
Nozaki 10.204
DTASelect 10.014
Thurlkill 10.116
EMBOSS 10.438
Sillero 10.218
Patrickios 10.204
IPC_peptide 10.35
IPC2_peptide 9.531
IPC2.peptide.svr19 8.414
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
13518
49
1212
245.8
27.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.95 ± 0.461
1.62 ± 0.162
5.363 ± 0.252
5.082 ± 0.226
3.04 ± 0.143
7.22 ± 0.293
1.886 ± 0.229
4.194 ± 0.203
4.905 ± 0.288
9.114 ± 0.32
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.929 ± 0.208
3.536 ± 0.265
3.869 ± 0.274
4.202 ± 0.344
6.828 ± 0.374
7.39 ± 0.295
6.791 ± 0.436
6.931 ± 0.209
1.502 ± 0.107
3.647 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here