Streptococcus virus C1
Average proteome isoelectric point is 5.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7Y3G1|Q7Y3G1_9CAUD Uncharacterized protein OS=Streptococcus virus C1 OX=230871 GN=orf1 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.16 TQEE5 pKa = 3.7 WYY7 pKa = 10.59 LVNFGLYY14 pKa = 7.08 EE15 pKa = 4.15 TKK17 pKa = 9.19 TQEE20 pKa = 3.65 MEE22 pKa = 3.93 TNSRR26 pKa = 11.84 YY27 pKa = 9.78 FEE29 pKa = 4.8 DD30 pKa = 3.71 KK31 pKa = 10.58 QAALDD36 pKa = 3.81 FFYY39 pKa = 10.89 TLANEE44 pKa = 5.51 GYY46 pKa = 9.51 YY47 pKa = 10.67 DD48 pKa = 3.56 WAHH51 pKa = 5.92 VYY53 pKa = 11.07 SNLEE57 pKa = 3.7 MEE59 pKa = 5.48 IILL62 pKa = 4.81
Molecular weight: 7.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.14
IPC2_protein 4.38
IPC_protein 4.19
Toseland 4.05
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 4.037
Grimsley 3.961
Solomon 4.113
Lehninger 4.062
Nozaki 4.253
DTASelect 4.329
Thurlkill 4.062
EMBOSS 4.012
Sillero 4.291
Patrickios 0.477
IPC_peptide 4.126
IPC2_peptide 4.291
IPC2.peptide.svr19 4.218
Protein with the highest isoelectric point:
>tr|Q7Y3F8|Q7Y3F8_9CAUD Uncharacterized protein OS=Streptococcus virus C1 OX=230871 GN=orf4 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.23 GDD4 pKa = 3.6 EE5 pKa = 4.11 EE6 pKa = 4.19 RR7 pKa = 11.84 TIKK10 pKa = 10.8 SLFPLFKK17 pKa = 10.97 YY18 pKa = 8.22 MANKK22 pKa = 9.78 RR23 pKa = 11.84 QRR25 pKa = 11.84 KK26 pKa = 8.25 KK27 pKa = 9.83 QLKK30 pKa = 7.14 QQYY33 pKa = 9.51 GVGHH37 pKa = 7.48 KK38 pKa = 7.19 YY39 pKa = 8.37 TPKK42 pKa = 10.62 LSQTQQKK49 pKa = 9.39 QADD52 pKa = 3.91 FLKK55 pKa = 11.03 SIGQKK60 pKa = 7.95 FTNYY64 pKa = 6.98 QTVTIDD70 pKa = 3.06 KK71 pKa = 9.34 TYY73 pKa = 11.21 SKK75 pKa = 10.74 NQEE78 pKa = 4.3 LLDD81 pKa = 3.85 TANEE85 pKa = 3.91 ALHH88 pKa = 6.37 RR89 pKa = 11.84 LGIFFDD95 pKa = 4.2 GSEE98 pKa = 4.31 KK99 pKa = 10.48 IKK101 pKa = 10.53 LQQVTDD107 pKa = 3.68 DD108 pKa = 3.82 DD109 pKa = 3.83 LRR111 pKa = 11.84 YY112 pKa = 9.89 IINKK116 pKa = 7.93 LQPLLEE122 pKa = 4.51 SVTMRR127 pKa = 11.84 YY128 pKa = 9.97 KK129 pKa = 10.77 KK130 pKa = 10.46 FLTNTYY136 pKa = 9.98 RR137 pKa = 11.84 SNNRR141 pKa = 11.84 DD142 pKa = 3.27 YY143 pKa = 11.41 RR144 pKa = 11.84 LDD146 pKa = 3.48 WLLKK150 pKa = 9.69 SAISKK155 pKa = 9.92 KK156 pKa = 10.42 LKK158 pKa = 9.66 NAQTVRR164 pKa = 11.84 GLVVAINKK172 pKa = 8.13 MDD174 pKa = 4.23 RR175 pKa = 11.84 DD176 pKa = 3.77 FKK178 pKa = 11.31 EE179 pKa = 3.7 YY180 pKa = 10.67 DD181 pKa = 2.96 KK182 pKa = 11.28 KK183 pKa = 11.01 LRR185 pKa = 11.84 KK186 pKa = 9.31 SSKK189 pKa = 9.27 QGNPFGFVVVKK200 pKa = 9.34 YY201 pKa = 11.05 SEE203 pKa = 4.17 MGLMM207 pKa = 4.43
Molecular weight: 24.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.082
IPC2_protein 9.443
IPC_protein 9.355
Toseland 10.218
ProMoST 9.794
Dawson 10.365
Bjellqvist 9.97
Wikipedia 10.496
Rodwell 11.008
Grimsley 10.409
Solomon 10.379
Lehninger 10.35
Nozaki 10.175
DTASelect 9.97
Thurlkill 10.218
EMBOSS 10.599
Sillero 10.262
Patrickios 10.687
IPC_peptide 10.379
IPC2_peptide 8.39
IPC2.peptide.svr19 8.479
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
0
20
4842
51
784
242.1
27.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.452 ± 0.742
0.599 ± 0.151
6.402 ± 0.461
6.568 ± 0.587
4.461 ± 0.345
5.7 ± 0.589
2.065 ± 0.259
6.981 ± 0.487
7.476 ± 0.742
8.633 ± 0.359
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.788 ± 0.155
7.993 ± 0.545
2.396 ± 0.338
4.048 ± 0.322
3.036 ± 0.293
6.402 ± 0.476
6.34 ± 0.411
5.927 ± 0.271
1.115 ± 0.198
5.618 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here