Streptomyces caelestis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6882 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N0S4Y0|A0A0N0S4Y0_9ACTN Methionine synthase OS=Streptomyces caelestis OX=36816 GN=ADK41_36415 PE=4 SV=1
MM1 pKa = 7.57SVQQEE6 pKa = 4.13AGVGGEE12 pKa = 4.33ALEE15 pKa = 4.1VWIDD19 pKa = 3.41QDD21 pKa = 3.99LCTGDD26 pKa = 5.01GICAQYY32 pKa = 10.9APEE35 pKa = 4.23VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.63GLAYY46 pKa = 10.45VKK48 pKa = 10.67SPQDD52 pKa = 3.3EE53 pKa = 4.28LLQDD57 pKa = 3.95EE58 pKa = 5.18GATTPVPLPLLTDD71 pKa = 3.6VVDD74 pKa = 4.12SAKK77 pKa = 10.15EE78 pKa = 3.93CPGEE82 pKa = 4.38CIHH85 pKa = 6.3VRR87 pKa = 11.84RR88 pKa = 11.84VSDD91 pKa = 3.56GVEE94 pKa = 4.16VYY96 pKa = 10.91GPDD99 pKa = 3.28AGG101 pKa = 4.06

Molecular weight: 10.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8QJF8|A0A0M8QJF8_9ACTN AMP-dependent synthetase OS=Streptomyces caelestis OX=36816 GN=ADK41_35800 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84ARR4 pKa = 11.84GRR6 pKa = 11.84RR7 pKa = 11.84INRR10 pKa = 11.84KK11 pKa = 8.72RR12 pKa = 11.84VTRR15 pKa = 11.84LMRR18 pKa = 11.84VNHH21 pKa = 6.57IIGRR25 pKa = 11.84HH26 pKa = 4.09LRR28 pKa = 11.84KK29 pKa = 9.78KK30 pKa = 10.44KK31 pKa = 10.21RR32 pKa = 3.33

Molecular weight: 4.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6882

0

6882

2213699

25

8129

321.7

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.628 ± 0.047

0.792 ± 0.008

6.142 ± 0.026

5.921 ± 0.032

2.663 ± 0.019

9.606 ± 0.03

2.371 ± 0.016

2.855 ± 0.022

2.004 ± 0.025

10.341 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.67 ± 0.012

1.658 ± 0.015

6.192 ± 0.03

2.542 ± 0.016

8.548 ± 0.036

4.838 ± 0.021

6.083 ± 0.026

8.629 ± 0.03

1.499 ± 0.012

2.019 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski