Amino acid dipepetide frequency for Streptomyces caelestis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.072AlaAla: 21.072 ± 0.164	1.12AlaCys: 1.12 ± 0.026	8.553AlaAsp: 8.553 ± 0.067	8.985AlaGlu: 8.985 ± 0.099	3.504AlaPhe: 3.504 ± 0.038	13.561AlaGly: 13.561 ± 0.096	3.061AlaHis: 3.061 ± 0.044	2.962AlaIle: 2.962 ± 0.041	2.526AlaLys: 2.526 ± 0.05	14.886AlaLeu: 14.886 ± 0.117	2.346AlaMet: 2.346 ± 0.033	1.736AlaAsn: 1.736 ± 0.033	7.517AlaPro: 7.517 ± 0.077	3.472AlaGln: 3.472 ± 0.049	11.113AlaArg: 11.113 ± 0.09	5.747AlaSer: 5.747 ± 0.049	6.692AlaThr: 6.692 ± 0.062	12.827AlaVal: 12.827 ± 0.088	1.859AlaTrp: 1.859 ± 0.032	2.741AlaTyr: 2.741 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	1.138CysAla: 1.138 ± 0.023	0.093CysCys: 0.093 ± 0.007	0.474CysAsp: 0.474 ± 0.014	0.406CysGlu: 0.406 ± 0.013	0.216CysPhe: 0.216 ± 0.009	0.96CysGly: 0.96 ± 0.021	0.201CysHis: 0.201 ± 0.01	0.141CysIle: 0.141 ± 0.009	0.104CysLys: 0.104 ± 0.007	0.75CysLeu: 0.75 ± 0.023	0.11CysMet: 0.11 ± 0.007	0.126CysAsn: 0.126 ± 0.007	0.5CysPro: 0.5 ± 0.016	0.142CysGln: 0.142 ± 0.007	0.655CysArg: 0.655 ± 0.019	0.444CysSer: 0.444 ± 0.015	0.5CysThr: 0.5 ± 0.017	0.695CysVal: 0.695 ± 0.018	0.12CysTrp: 0.12 ± 0.008	0.141CysTyr: 0.141 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.063AspAla: 8.063 ± 0.08	0.438AspCys: 0.438 ± 0.016	3.875AspAsp: 3.875 ± 0.049	4.1AspGlu: 4.1 ± 0.045	1.624AspPhe: 1.624 ± 0.031	6.848AspGly: 6.848 ± 0.068	1.494AspHis: 1.494 ± 0.028	1.713AspIle: 1.713 ± 0.032	1.086AspLys: 1.086 ± 0.027	6.33AspLeu: 6.33 ± 0.058	0.805AspMet: 0.805 ± 0.018	0.903AspAsn: 0.903 ± 0.019	4.612AspPro: 4.612 ± 0.055	1.401AspGln: 1.401 ± 0.028	5.351AspArg: 5.351 ± 0.056	2.382AspSer: 2.382 ± 0.036	3.359AspThr: 3.359 ± 0.042	4.921AspVal: 4.921 ± 0.051	1.058AspTrp: 1.058 ± 0.023	1.058AspTyr: 1.058 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.627GluAla: 7.627 ± 0.08	0.386GluCys: 0.386 ± 0.014	3.163GluAsp: 3.163 ± 0.043	4.03GluGlu: 4.03 ± 0.052	1.429GluPhe: 1.429 ± 0.03	4.649GluGly: 4.649 ± 0.047	1.638GluHis: 1.638 ± 0.03	2.244GluIle: 2.244 ± 0.037	1.499GluLys: 1.499 ± 0.032	6.8GluLeu: 6.8 ± 0.068	0.875GluMet: 0.875 ± 0.021	1.106GluAsn: 1.106 ± 0.026	3.609GluPro: 3.609 ± 0.047	2.185GluGln: 2.185 ± 0.037	5.972GluArg: 5.972 ± 0.063	2.548GluSer: 2.548 ± 0.038	2.971GluThr: 2.971 ± 0.034	4.588GluVal: 4.588 ± 0.045	0.756GluTrp: 0.756 ± 0.019	1.132GluTyr: 1.132 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.578PheAla: 3.578 ± 0.043	0.259PheCys: 0.259 ± 0.011	1.955PheAsp: 1.955 ± 0.033	1.437PheGlu: 1.437 ± 0.023	0.83PhePhe: 0.83 ± 0.021	2.906PheGly: 2.906 ± 0.045	0.627PheHis: 0.627 ± 0.016	0.61PheIle: 0.61 ± 0.019	0.471PheLys: 0.471 ± 0.015	2.599PheLeu: 2.599 ± 0.043	0.393PheMet: 0.393 ± 0.014	0.528PheAsn: 0.528 ± 0.017	1.361PhePro: 1.361 ± 0.028	0.635PheGln: 0.635 ± 0.018	1.903PheArg: 1.903 ± 0.034	1.369PheSer: 1.369 ± 0.025	1.979PheThr: 1.979 ± 0.032	2.262PheVal: 2.262 ± 0.033	0.405PheTrp: 0.405 ± 0.014	0.519PheTyr: 0.519 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	11.745GlyAla: 11.745 ± 0.079	0.828GlyCys: 0.828 ± 0.021	5.48GlyAsp: 5.48 ± 0.056	5.51GlyGlu: 5.51 ± 0.053	2.874GlyPhe: 2.874 ± 0.041	9.178GlyGly: 9.178 ± 0.104	2.482GlyHis: 2.482 ± 0.036	3.18GlyIle: 3.18 ± 0.035	2.238GlyLys: 2.238 ± 0.043	9.42GlyLeu: 9.42 ± 0.075	1.958GlyMet: 1.958 ± 0.027	1.696GlyAsn: 1.696 ± 0.038	5.461GlyPro: 5.461 ± 0.07	2.462GlyGln: 2.462 ± 0.035	8.471GlyArg: 8.471 ± 0.082	5.125GlySer: 5.125 ± 0.053	6.374GlyThr: 6.374 ± 0.064	7.739GlyVal: 7.739 ± 0.071	1.681GlyTrp: 1.681 ± 0.033	2.157GlyTyr: 2.157 ± 0.031	0.0GlyXaa: 0.0 ± 0.0
His	2.845HisAla: 2.845 ± 0.048	0.215HisCys: 0.215 ± 0.011	1.392HisAsp: 1.392 ± 0.025	1.281HisGlu: 1.281 ± 0.026	0.635HisPhe: 0.635 ± 0.016	2.573HisGly: 2.573 ± 0.035	0.726HisHis: 0.726 ± 0.021	0.627HisIle: 0.627 ± 0.017	0.346HisLys: 0.346 ± 0.013	2.511HisLeu: 2.511 ± 0.036	0.333HisMet: 0.333 ± 0.011	0.345HisAsn: 0.345 ± 0.013	1.87HisPro: 1.87 ± 0.031	0.628HisGln: 0.628 ± 0.016	2.306HisArg: 2.306 ± 0.036	0.977HisSer: 0.977 ± 0.024	1.433HisThr: 1.433 ± 0.027	1.796HisVal: 1.796 ± 0.028	0.39HisTrp: 0.39 ± 0.014	0.477HisTyr: 0.477 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.339IleAla: 4.339 ± 0.054	0.263IleCys: 0.263 ± 0.011	2.052IleAsp: 2.052 ± 0.037	1.886IleGlu: 1.886 ± 0.035	0.568IlePhe: 0.568 ± 0.017	3.311IleGly: 3.311 ± 0.043	0.565IleHis: 0.565 ± 0.016	0.726IleIle: 0.726 ± 0.023	0.627IleLys: 0.627 ± 0.019	2.194IleLeu: 2.194 ± 0.039	0.412IleMet: 0.412 ± 0.017	0.566IleAsn: 0.566 ± 0.018	1.554IlePro: 1.554 ± 0.025	0.599IleGln: 0.599 ± 0.018	2.19IleArg: 2.19 ± 0.03	1.417IleSer: 1.417 ± 0.024	2.045IleThr: 2.045 ± 0.033	2.51IleVal: 2.51 ± 0.039	0.3IleTrp: 0.3 ± 0.011	0.424IleTyr: 0.424 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.752LysAla: 2.752 ± 0.051	0.105LysCys: 0.105 ± 0.007	1.28LysAsp: 1.28 ± 0.03	1.231LysGlu: 1.231 ± 0.026	0.404LysPhe: 0.404 ± 0.018	1.787LysGly: 1.787 ± 0.038	0.405LysHis: 0.405 ± 0.017	0.76LysIle: 0.76 ± 0.022	0.763LysLys: 0.763 ± 0.027	1.826LysLeu: 1.826 ± 0.035	0.36LysMet: 0.36 ± 0.012	0.471LysAsn: 0.471 ± 0.018	1.242LysPro: 1.242 ± 0.033	0.611LysGln: 0.611 ± 0.017	1.427LysArg: 1.427 ± 0.034	1.031LysSer: 1.031 ± 0.026	1.191LysThr: 1.191 ± 0.026	1.723LysVal: 1.723 ± 0.031	0.258LysTrp: 0.258 ± 0.013	0.418LysTyr: 0.418 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.097LeuAla: 15.097 ± 0.12	0.856LeuCys: 0.856 ± 0.019	6.891LeuAsp: 6.891 ± 0.067	4.946LeuGlu: 4.946 ± 0.05	2.574LeuPhe: 2.574 ± 0.038	9.111LeuGly: 9.111 ± 0.084	2.373LeuHis: 2.373 ± 0.033	2.986LeuIle: 2.986 ± 0.043	1.973LeuLys: 1.973 ± 0.037	11.361LeuLeu: 11.361 ± 0.098	1.604LeuMet: 1.604 ± 0.031	1.582LeuAsn: 1.582 ± 0.03	6.58LeuPro: 6.58 ± 0.069	2.058LeuGln: 2.058 ± 0.035	9.072LeuArg: 9.072 ± 0.085	5.213LeuSer: 5.213 ± 0.046	7.077LeuThr: 7.077 ± 0.068	8.882LeuVal: 8.882 ± 0.076	1.323LeuTrp: 1.323 ± 0.027	1.851LeuTyr: 1.851 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	2.143MetAla: 2.143 ± 0.032	0.138MetCys: 0.138 ± 0.008	0.89MetAsp: 0.89 ± 0.022	0.795MetGlu: 0.795 ± 0.021	0.455MetPhe: 0.455 ± 0.015	1.268MetGly: 1.268 ± 0.025	0.337MetHis: 0.337 ± 0.012	0.58MetIle: 0.58 ± 0.018	0.371MetLys: 0.371 ± 0.014	1.624MetLeu: 1.624 ± 0.03	0.273MetMet: 0.273 ± 0.012	0.388MetAsn: 0.388 ± 0.012	1.059MetPro: 1.059 ± 0.024	0.401MetGln: 0.401 ± 0.016	1.5MetArg: 1.5 ± 0.022	1.197MetSer: 1.197 ± 0.023	1.456MetThr: 1.456 ± 0.027	1.295MetVal: 1.295 ± 0.028	0.218MetTrp: 0.218 ± 0.01	0.314MetTyr: 0.314 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.074AsnAla: 2.074 ± 0.03	0.155AsnCys: 0.155 ± 0.008	0.89AsnAsp: 0.89 ± 0.023	0.798AsnGlu: 0.798 ± 0.026	0.458AsnPhe: 0.458 ± 0.015	1.872AsnGly: 1.872 ± 0.033	0.377AsnHis: 0.377 ± 0.013	0.57AsnIle: 0.57 ± 0.018	0.378AsnLys: 0.378 ± 0.013	1.559AsnLeu: 1.559 ± 0.033	0.267AsnMet: 0.267 ± 0.013	0.37AsnAsn: 0.37 ± 0.014	1.283AsnPro: 1.283 ± 0.026	0.473AsnGln: 0.473 ± 0.018	1.27AsnArg: 1.27 ± 0.028	0.803AsnSer: 0.803 ± 0.022	1.02AsnThr: 1.02 ± 0.027	1.31AsnVal: 1.31 ± 0.028	0.284AsnTrp: 0.284 ± 0.012	0.365AsnTyr: 0.365 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.143ProAla: 9.143 ± 0.084	0.371ProCys: 0.371 ± 0.013	4.678ProAsp: 4.678 ± 0.059	4.505ProGlu: 4.505 ± 0.045	1.499ProPhe: 1.499 ± 0.024	6.986ProGly: 6.986 ± 0.084	1.441ProHis: 1.441 ± 0.028	1.055ProIle: 1.055 ± 0.021	1.084ProLys: 1.084 ± 0.028	5.398ProLeu: 5.398 ± 0.051	0.973ProMet: 0.973 ± 0.02	0.804ProAsn: 0.804 ± 0.018	3.733ProPro: 3.733 ± 0.061	1.588ProGln: 1.588 ± 0.032	4.361ProArg: 4.361 ± 0.057	3.139ProSer: 3.139 ± 0.047	3.046ProThr: 3.046 ± 0.038	5.836ProVal: 5.836 ± 0.058	0.888ProTrp: 0.888 ± 0.022	1.39ProTyr: 1.39 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.506GlnAla: 3.506 ± 0.043	0.154GlnCys: 0.154 ± 0.009	1.376GlnAsp: 1.376 ± 0.027	1.466GlnGlu: 1.466 ± 0.026	0.592GlnPhe: 0.592 ± 0.016	2.181GlnGly: 2.181 ± 0.034	0.611GlnHis: 0.611 ± 0.018	0.878GlnIle: 0.878 ± 0.022	0.575GlnLys: 0.575 ± 0.02	2.687GlnLeu: 2.687 ± 0.041	0.446GlnMet: 0.446 ± 0.014	0.466GlnAsn: 0.466 ± 0.014	1.502GlnPro: 1.502 ± 0.031	1.134GlnGln: 1.134 ± 0.036	2.264GlnArg: 2.264 ± 0.035	1.132GlnSer: 1.132 ± 0.023	1.215GlnThr: 1.215 ± 0.027	2.222GlnVal: 2.222 ± 0.033	0.449GlnTrp: 0.449 ± 0.015	0.566GlnTyr: 0.566 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	10.65ArgAla: 10.65 ± 0.096	0.631ArgCys: 0.631 ± 0.018	4.586ArgAsp: 4.586 ± 0.049	5.18ArgGlu: 5.18 ± 0.05	2.422ArgPhe: 2.422 ± 0.036	6.275ArgGly: 6.275 ± 0.059	2.344ArgHis: 2.344 ± 0.033	3.211ArgIle: 3.211 ± 0.041	1.642ArgLys: 1.642 ± 0.031	9.492ArgLeu: 9.492 ± 0.096	1.785ArgMet: 1.785 ± 0.03	1.404ArgAsn: 1.404 ± 0.028	5.552ArgPro: 5.552 ± 0.055	2.259ArgGln: 2.259 ± 0.034	8.548ArgArg: 8.548 ± 0.09	4.195ArgSer: 4.195 ± 0.038	5.767ArgThr: 5.767 ± 0.056	6.337ArgVal: 6.337 ± 0.053	1.401ArgTrp: 1.401 ± 0.027	1.801ArgTyr: 1.801 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.395SerAla: 6.395 ± 0.058	0.399SerCys: 0.399 ± 0.016	2.58SerAsp: 2.58 ± 0.035	2.332SerGlu: 2.332 ± 0.039	1.469SerPhe: 1.469 ± 0.027	5.805SerGly: 5.805 ± 0.05	0.994SerHis: 0.994 ± 0.02	1.187SerIle: 1.187 ± 0.025	0.934SerLys: 0.934 ± 0.025	4.767SerLeu: 4.767 ± 0.048	0.944SerMet: 0.944 ± 0.021	0.793SerAsn: 0.793 ± 0.021	3.103SerPro: 3.103 ± 0.041	1.1SerGln: 1.1 ± 0.026	3.781SerArg: 3.781 ± 0.046	2.642SerSer: 2.642 ± 0.043	2.87SerThr: 2.87 ± 0.043	4.269SerVal: 4.269 ± 0.041	0.868SerTrp: 0.868 ± 0.02	1.146SerTyr: 1.146 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	9.157ThrAla: 9.157 ± 0.087	0.414ThrCys: 0.414 ± 0.013	3.678ThrAsp: 3.678 ± 0.043	3.326ThrGlu: 3.326 ± 0.036	1.517ThrPhe: 1.517 ± 0.025	6.977ThrGly: 6.977 ± 0.069	1.206ThrHis: 1.206 ± 0.025	1.485ThrIle: 1.485 ± 0.027	1.035ThrLys: 1.035 ± 0.027	5.609ThrLeu: 5.609 ± 0.053	0.913ThrMet: 0.913 ± 0.02	0.913ThrAsn: 0.913 ± 0.019	4.038ThrPro: 4.038 ± 0.048	1.207ThrGln: 1.207 ± 0.025	4.121ThrArg: 4.121 ± 0.045	2.957ThrSer: 2.957 ± 0.035	3.729ThrThr: 3.729 ± 0.047	6.315ThrVal: 6.315 ± 0.059	0.902ThrTrp: 0.902 ± 0.02	1.329ThrTyr: 1.329 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.588ValAla: 10.588 ± 0.077	0.764ValCys: 0.764 ± 0.017	5.242ValAsp: 5.242 ± 0.055	4.995ValGlu: 4.995 ± 0.054	2.482ValPhe: 2.482 ± 0.034	6.444ValGly: 6.444 ± 0.061	2.091ValHis: 2.091 ± 0.032	2.681ValIle: 2.681 ± 0.039	1.671ValLys: 1.671 ± 0.034	9.874ValLeu: 9.874 ± 0.085	1.39ValMet: 1.39 ± 0.027	1.596ValAsn: 1.596 ± 0.031	5.557ValPro: 5.557 ± 0.056	1.948ValGln: 1.948 ± 0.033	7.906ValArg: 7.906 ± 0.066	4.243ValSer: 4.243 ± 0.045	5.874ValThr: 5.874 ± 0.046	8.187ValVal: 8.187 ± 0.07	1.175ValTrp: 1.175 ± 0.026	1.581ValTyr: 1.581 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.653TrpAla: 1.653 ± 0.03	0.159TrpCys: 0.159 ± 0.009	0.839TrpAsp: 0.839 ± 0.023	0.76TrpGlu: 0.76 ± 0.018	0.481TrpPhe: 0.481 ± 0.014	1.079TrpGly: 1.079 ± 0.021	0.353TrpHis: 0.353 ± 0.012	0.513TrpIle: 0.513 ± 0.017	0.351TrpLys: 0.351 ± 0.013	1.709TrpLeu: 1.709 ± 0.03	0.28TrpMet: 0.28 ± 0.012	0.412TrpAsn: 0.412 ± 0.015	0.805TrpPro: 0.805 ± 0.021	0.571TrpGln: 0.571 ± 0.017	1.387TrpArg: 1.387 ± 0.026	0.918TrpSer: 0.918 ± 0.021	1.081TrpThr: 1.081 ± 0.022	0.954TrpVal: 0.954 ± 0.019	0.32TrpTrp: 0.32 ± 0.013	0.368TrpTyr: 0.368 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.714TyrAla: 2.714 ± 0.041	0.168TyrCys: 0.168 ± 0.009	1.546TyrAsp: 1.546 ± 0.029	1.24TyrGlu: 1.24 ± 0.026	0.592TyrPhe: 0.592 ± 0.017	2.288TyrGly: 2.288 ± 0.036	0.383TyrHis: 0.383 ± 0.014	0.439TyrIle: 0.439 ± 0.014	0.369TyrLys: 0.369 ± 0.014	2.015TyrLeu: 2.015 ± 0.03	0.234TyrMet: 0.234 ± 0.009	0.37TyrAsn: 0.37 ± 0.013	0.978TyrPro: 0.978 ± 0.021	0.548TyrGln: 0.548 ± 0.017	1.886TyrArg: 1.886 ± 0.034	0.9TyrSer: 0.9 ± 0.018	1.147TyrThr: 1.147 ± 0.025	1.619TyrVal: 1.619 ± 0.024	0.34TyrTrp: 0.34 ± 0.013	0.412TyrTyr: 0.412 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6882 proteins (2213699 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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