Alkalimonas amylolytica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Alkalimonas

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3615 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H4CKN6|A0A1H4CKN6_ALKAM N-formylglutamate deformylase OS=Alkalimonas amylolytica OX=152573 GN=SAMN04488051_104195 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.24KK3 pKa = 10.19LQVALLTAGLFSVGSVQASPISFDD27 pKa = 3.27FFNAHH32 pKa = 5.66EE33 pKa = 4.33TTEE36 pKa = 4.03IQQTGSLSMFDD47 pKa = 4.22DD48 pKa = 4.0ALGNLTSVTLNLLGEE63 pKa = 4.89SISSTILDD71 pKa = 3.65NQASNGQTFSYY82 pKa = 10.47LSQLSFFFDD91 pKa = 3.66ILGLSNNITLPAPTFITTLATTNGFATLASGTQLDD126 pKa = 4.46LGTNTEE132 pKa = 3.75QGAYY136 pKa = 10.22SFTFTDD142 pKa = 3.83PMDD145 pKa = 3.43IAAFVGAGSFTVNCNTLSSSGFTGGGGNIDD175 pKa = 3.85TNQVTTGACGAEE187 pKa = 4.26VIYY190 pKa = 10.52SYY192 pKa = 9.38NTPTTSISEE201 pKa = 4.2PMTLWLFGAGLFGFAAMRR219 pKa = 11.84RR220 pKa = 11.84FSS222 pKa = 3.28

Molecular weight: 23.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H4D2P6|A0A1H4D2P6_ALKAM DNA gyrase subunit B OS=Alkalimonas amylolytica OX=152573 GN=gyrB PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.34RR13 pKa = 11.84KK14 pKa = 9.1RR15 pKa = 11.84VHH17 pKa = 6.26GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.4NGRR29 pKa = 11.84QVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.87GRR40 pKa = 11.84KK41 pKa = 8.66RR42 pKa = 11.84LAVV45 pKa = 3.41

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3615

0

3615

1228723

39

2934

339.9

37.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.971 ± 0.045

1.029 ± 0.014

5.178 ± 0.033

5.583 ± 0.034

3.992 ± 0.023

6.758 ± 0.037

2.448 ± 0.019

5.149 ± 0.03

3.706 ± 0.039

12.029 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.017

3.294 ± 0.026

4.403 ± 0.028

6.546 ± 0.059

5.508 ± 0.033

6.267 ± 0.034

4.827 ± 0.027

6.55 ± 0.037

1.472 ± 0.019

2.916 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski