Faeces associated gemycircularvirus 16
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160HWN0|A0A160HWN0_9VIRU Replication-associated protein OS=Faeces associated gemycircularvirus 16 OX=1843736 PE=3 SV=1
MM1 pKa = 6.93 TQMLFCNSKK10 pKa = 10.64 YY11 pKa = 11.1 VLLTYY16 pKa = 9.13 AQCGDD21 pKa = 3.67 LDD23 pKa = 3.59 EE24 pKa = 4.86 WAVSDD29 pKa = 4.21 HH30 pKa = 6.46 LSSLGAEE37 pKa = 4.23 CIVARR42 pKa = 11.84 EE43 pKa = 4.04 LHH45 pKa = 6.0 PTTGGVHH52 pKa = 5.86 LHH54 pKa = 5.79 VFVDD58 pKa = 4.54 FGRR61 pKa = 11.84 KK62 pKa = 7.9 FRR64 pKa = 11.84 SRR66 pKa = 11.84 RR67 pKa = 11.84 VDD69 pKa = 2.87 IFDD72 pKa = 3.52 VEE74 pKa = 4.44 GRR76 pKa = 11.84 HH77 pKa = 5.97 PNVVPSKK84 pKa = 8.26 GTPEE88 pKa = 3.8 KK89 pKa = 10.85 GYY91 pKa = 10.88 DD92 pKa = 3.43 YY93 pKa = 10.61 AIKK96 pKa = 10.61 DD97 pKa = 3.82 GEE99 pKa = 4.57 VVAGGLEE106 pKa = 4.08 RR107 pKa = 11.84 PRR109 pKa = 11.84 GGRR112 pKa = 11.84 PGTVSGLEE120 pKa = 4.4 AIAHH124 pKa = 5.74 LCEE127 pKa = 4.35 TQDD130 pKa = 3.61 EE131 pKa = 4.36 FLDD134 pKa = 3.72 IYY136 pKa = 11.48 GEE138 pKa = 3.96 VDD140 pKa = 2.94 TRR142 pKa = 11.84 GLIKK146 pKa = 10.77 NFANVRR152 pKa = 11.84 SYY154 pKa = 11.77 AKK156 pKa = 9.08 WRR158 pKa = 11.84 YY159 pKa = 10.07 AGTLPKK165 pKa = 10.45 YY166 pKa = 9.07 EE167 pKa = 4.12 SPYY170 pKa = 10.52 SIGEE174 pKa = 3.78 FRR176 pKa = 11.84 RR177 pKa = 11.84 GSDD180 pKa = 5.52 GRR182 pKa = 11.84 DD183 pKa = 2.71 QWLAQSGIRR192 pKa = 11.84 SGDD195 pKa = 3.58 LVVSKK200 pKa = 10.33 CFQQ203 pKa = 3.47
Molecular weight: 22.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.944
IPC2_protein 5.944
IPC_protein 6.02
Toseland 6.338
ProMoST 6.338
Dawson 6.338
Bjellqvist 6.3
Wikipedia 6.351
Rodwell 6.326
Grimsley 6.453
Solomon 6.338
Lehninger 6.338
Nozaki 6.605
DTASelect 6.781
Thurlkill 6.81
EMBOSS 6.781
Sillero 6.707
Patrickios 4.075
IPC_peptide 6.351
IPC2_peptide 6.664
IPC2.peptide.svr19 6.623
Protein with the highest isoelectric point:
>tr|A0A168MG69|A0A168MG69_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 16 OX=1843736 PE=4 SV=1
MM1 pKa = 7.65 PRR3 pKa = 11.84 FKK5 pKa = 10.87 NRR7 pKa = 11.84 RR8 pKa = 11.84 TRR10 pKa = 11.84 FARR13 pKa = 11.84 KK14 pKa = 8.7 AKK16 pKa = 9.69 VWFKK20 pKa = 11.1 EE21 pKa = 3.54 KK22 pKa = 10.55 LPRR25 pKa = 11.84 STRR28 pKa = 11.84 RR29 pKa = 11.84 VTKK32 pKa = 8.3 RR33 pKa = 11.84 TYY35 pKa = 9.6 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 PMTKK43 pKa = 9.84 EE44 pKa = 3.59 VDD46 pKa = 3.58 PQPQRR51 pKa = 11.84 RR52 pKa = 11.84 SRR54 pKa = 11.84 KK55 pKa = 8.15 KK56 pKa = 8.5 GCHH59 pKa = 5.87 ASVTNPTLDD68 pKa = 3.68 PLIASSASPAILGSGRR84 pKa = 11.84 PAPSGAPAYY93 pKa = 7.21 TYY95 pKa = 9.09 CIPWIATRR103 pKa = 11.84 RR104 pKa = 11.84 KK105 pKa = 8.0 PANVGNSAMAQLGTGTPYY123 pKa = 10.31 MKK125 pKa = 10.55 GLSEE129 pKa = 5.29 NITISTTGAAPWQWRR144 pKa = 11.84 RR145 pKa = 11.84 VCFTFKK151 pKa = 10.44 SKK153 pKa = 11.16 YY154 pKa = 10.26 LIEE157 pKa = 4.3 QFTISQGDD165 pKa = 3.68 DD166 pKa = 3.9 LIDD169 pKa = 4.34 FNHH172 pKa = 7.29 DD173 pKa = 3.44 PFFSNGYY180 pKa = 8.6 NDD182 pKa = 3.07 SSGVFRR188 pKa = 11.84 PMYY191 pKa = 10.53 DD192 pKa = 2.97 LASYY196 pKa = 9.53 YY197 pKa = 10.78 GKK199 pKa = 10.11 HH200 pKa = 5.66 NNPLKK205 pKa = 10.54 FRR207 pKa = 11.84 ARR209 pKa = 11.84 SPQHH213 pKa = 5.33 TLGFSTLCSVVAAQQLPGQPTQADD237 pKa = 4.57 YY238 pKa = 11.49 INVMTAKK245 pKa = 9.69 TDD247 pKa = 3.54 NTEE250 pKa = 3.74 VTIKK254 pKa = 10.45 YY255 pKa = 9.9 DD256 pKa = 3.34 KK257 pKa = 10.56 TVTIASGNEE266 pKa = 3.31 AGVQRR271 pKa = 11.84 NYY273 pKa = 10.6 RR274 pKa = 11.84 RR275 pKa = 11.84 YY276 pKa = 9.74 HH277 pKa = 6.11 PMNKK281 pKa = 8.41 TLVYY285 pKa = 10.71 DD286 pKa = 3.77 SLEE289 pKa = 3.9 QGSNSNYY296 pKa = 10.58 DD297 pKa = 3.49 SMSTEE302 pKa = 4.37 AKK304 pKa = 10.09 PGMGDD309 pKa = 3.59 YY310 pKa = 10.92 YY311 pKa = 11.56 VVDD314 pKa = 4.59 FFQARR319 pKa = 11.84 YY320 pKa = 8.86 LADD323 pKa = 4.58 ADD325 pKa = 4.18 DD326 pKa = 4.88 GNLIFDD332 pKa = 4.66 PRR334 pKa = 11.84 ATLYY338 pKa = 8.62 WHH340 pKa = 6.56 EE341 pKa = 4.23 RR342 pKa = 3.47
Molecular weight: 38.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.888
IPC2_protein 9.472
IPC_protein 9.648
Toseland 9.897
ProMoST 9.75
Dawson 10.175
Bjellqvist 9.897
Wikipedia 10.379
Rodwell 10.423
Grimsley 10.262
Solomon 10.189
Lehninger 10.145
Nozaki 9.926
DTASelect 9.882
Thurlkill 9.999
EMBOSS 10.321
Sillero 10.087
Patrickios 5.652
IPC_peptide 10.189
IPC2_peptide 8.712
IPC2.peptide.svr19 8.489
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
881
203
342
293.7
33.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.151 ± 0.545
1.93 ± 0.369
6.47 ± 0.447
4.654 ± 0.995
4.881 ± 0.368
9.194 ± 1.483
2.497 ± 0.36
3.973 ± 0.098
5.108 ± 0.367
6.583 ± 0.662
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.93 ± 0.386
3.519 ± 0.861
5.335 ± 0.825
3.065 ± 0.538
8.059 ± 0.36
6.924 ± 0.361
5.675 ± 1.226
6.47 ± 0.913
1.93 ± 0.356
4.654 ± 0.456
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here