Elstera litoralis
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3298 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F3IHN2|A0A0F3IHN2_9PROT Transposase (Fragment) OS=Elstera litoralis OX=552518 GN=VZ95_20870 PE=4 SV=1
MM1 pKa = 7.73 IGGRR5 pKa = 11.84 DD6 pKa = 3.57 NDD8 pKa = 4.33 TLTGGAGGDD17 pKa = 3.29 TFYY20 pKa = 11.11 FGSADD25 pKa = 3.27 GAAADD30 pKa = 4.7 LSAANSGVDD39 pKa = 3.55 TITDD43 pKa = 4.13 FTATDD48 pKa = 3.6 NLCFNVSALGMTSNVGTMKK67 pKa = 10.54 VATLSSGGATLTSLANTATAGNVDD91 pKa = 4.55 AVILLNTTGFASYY104 pKa = 10.52 AAAQAEE110 pKa = 4.53 LNATAAAITDD120 pKa = 3.71 NSGSAIVLWYY130 pKa = 10.75 SSVDD134 pKa = 3.58 SKK136 pKa = 11.5 IHH138 pKa = 5.65 ITHH141 pKa = 7.04 DD142 pKa = 3.4 TDD144 pKa = 3.0 ISTGAGTGTEE154 pKa = 3.8 IGIIGNSTSATLAALTGSNFTMIAA178 pKa = 3.14
Molecular weight: 17.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.656
IPC_protein 3.643
Toseland 3.414
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.681
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.605
Nozaki 3.808
DTASelect 4.113
Thurlkill 3.503
EMBOSS 3.681
Sillero 3.783
Patrickios 1.888
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A0F3IP59|A0A0F3IP59_9PROT Membrane protein (Fragment) OS=Elstera litoralis OX=552518 GN=VZ95_17300 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 EE3 pKa = 3.96 NLGTRR8 pKa = 11.84 QNLSGSVFGGLSALLIALTLAAQPVAAQQTQGASPRR44 pKa = 11.84 RR45 pKa = 11.84 PNPHH49 pKa = 7.36 PPRR52 pKa = 11.84 KK53 pKa = 8.28 PHH55 pKa = 6.42 PRR57 pKa = 11.84 SPQPKK62 pKa = 7.72 HH63 pKa = 5.98 RR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 RR67 pKa = 11.84 PRR69 pKa = 11.84 RR70 pKa = 11.84 QQ71 pKa = 2.83
Molecular weight: 7.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.281
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.018
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.126
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3298
0
3298
801678
26
1706
243.1
26.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.736 ± 0.064
0.805 ± 0.015
5.447 ± 0.033
5.205 ± 0.041
3.767 ± 0.03
8.474 ± 0.049
1.865 ± 0.023
5.261 ± 0.035
3.278 ± 0.043
11.307 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.288 ± 0.021
2.501 ± 0.025
5.403 ± 0.035
3.421 ± 0.026
6.779 ± 0.051
5.158 ± 0.03
5.639 ± 0.042
7.108 ± 0.037
1.313 ± 0.021
2.242 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here