Hubei odonate virus 12
Average proteome isoelectric point is 8.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGF4|A0A1L3KGF4_9VIRU Chropara_Vmeth domain-containing protein OS=Hubei odonate virus 12 OX=1922993 PE=4 SV=1
MM1 pKa = 7.54 EE2 pKa = 5.49 FDD4 pKa = 3.98 EE5 pKa = 5.04 VGISNAEE12 pKa = 4.14 WIANWRR18 pKa = 11.84 WVRR21 pKa = 11.84 NKK23 pKa = 10.05 VEE25 pKa = 4.15 YY26 pKa = 11.14 VFDD29 pKa = 4.57 LDD31 pKa = 3.21 KK32 pKa = 11.3 HH33 pKa = 5.47 RR34 pKa = 11.84 AIAEE38 pKa = 4.09 AEE40 pKa = 3.86 AAEE43 pKa = 4.16 ALRR46 pKa = 11.84 LLAEE50 pKa = 4.31 YY51 pKa = 10.58 DD52 pKa = 3.72 DD53 pKa = 5.03 NSFGTHH59 pKa = 4.91 YY60 pKa = 10.42 RR61 pKa = 11.84 QASRR65 pKa = 11.84 LAKK68 pKa = 9.71 QCAASAVTLKK78 pKa = 11.1 DD79 pKa = 3.21 KK80 pKa = 10.87 TCDD83 pKa = 3.63 FLYY86 pKa = 9.16 KK87 pKa = 10.13 TYY89 pKa = 10.54 EE90 pKa = 4.15 ALTAPPRR97 pKa = 11.84 VVEE100 pKa = 3.89 VLPWYY105 pKa = 10.36 APSRR109 pKa = 11.84 LARR112 pKa = 11.84 SSLRR116 pKa = 11.84 WCWQHH121 pKa = 7.33 KK122 pKa = 9.99 VILAGSVVASLIYY135 pKa = 10.14 TGFYY139 pKa = 10.18 LYY141 pKa = 10.68 YY142 pKa = 10.34 RR143 pKa = 11.84 NAKK146 pKa = 7.97 VVRR149 pKa = 11.84 VDD151 pKa = 3.73 SGDD154 pKa = 3.59 SPFEE158 pKa = 4.17 VNTLKK163 pKa = 10.87 DD164 pKa = 3.0 RR165 pKa = 11.84 WEE167 pKa = 4.3 LFSRR171 pKa = 11.84 TYY173 pKa = 10.87 NPPAYY178 pKa = 10.18 HH179 pKa = 7.59 PGRR182 pKa = 11.84 HH183 pKa = 4.95 MRR185 pKa = 11.84 LAAEE189 pKa = 4.11 RR190 pKa = 11.84 RR191 pKa = 11.84 HH192 pKa = 4.75 VEE194 pKa = 3.91 RR195 pKa = 11.84 FIVDD199 pKa = 3.16 IFVTHH204 pKa = 6.86 RR205 pKa = 11.84 MRR207 pKa = 11.84 FRR209 pKa = 11.84 DD210 pKa = 3.34 VGGSRR215 pKa = 11.84 NRR217 pKa = 11.84 WVEE220 pKa = 3.79 SSVYY224 pKa = 10.46 KK225 pKa = 10.2 HH226 pKa = 6.34 LCFKK230 pKa = 10.32 EE231 pKa = 4.29 FSGFDD236 pKa = 2.84 ILRR239 pKa = 11.84 RR240 pKa = 11.84 QKK242 pKa = 10.85 RR243 pKa = 11.84 PITPFEE249 pKa = 3.98 EE250 pKa = 5.1 CGQSGQDD257 pKa = 3.14 CPQRR261 pKa = 11.84 HH262 pKa = 6.31 SIHH265 pKa = 6.32 GAMLVDD271 pKa = 3.31 VDD273 pKa = 5.01 YY274 pKa = 11.92 YY275 pKa = 10.86 MSNDD279 pKa = 3.4 DD280 pKa = 3.74 LAEE283 pKa = 4.19 VVTGPTFIVNHH294 pKa = 6.43 DD295 pKa = 3.92 FARR298 pKa = 11.84 GRR300 pKa = 11.84 QGEE303 pKa = 4.18 LGEE306 pKa = 4.29 EE307 pKa = 4.57 GMVEE311 pKa = 4.11 ARR313 pKa = 11.84 WHH315 pKa = 6.23 KK316 pKa = 10.93 EE317 pKa = 3.21 GDD319 pKa = 4.1 CVVMTSEE326 pKa = 5.33 DD327 pKa = 3.38 GTPYY331 pKa = 9.26 KK332 pKa = 10.53 HH333 pKa = 7.08 RR334 pKa = 11.84 WHH336 pKa = 6.16 TWSNEE341 pKa = 3.76 GIVVGKK347 pKa = 10.18 NGAFTYY353 pKa = 10.71 VRR355 pKa = 11.84 IGKK358 pKa = 8.89 LRR360 pKa = 11.84 TLQIYY365 pKa = 8.42 YY366 pKa = 9.91 AYY368 pKa = 8.46 PASGVYY374 pKa = 9.99 SGSDD378 pKa = 3.28 SCNLTWGGPEE388 pKa = 3.98 QIFCRR393 pKa = 11.84 NHH395 pKa = 6.42 AMDD398 pKa = 3.7 VFRR401 pKa = 11.84 RR402 pKa = 11.84 LEE404 pKa = 4.2 GEE406 pKa = 3.89 EE407 pKa = 4.29 EE408 pKa = 4.16 IYY410 pKa = 10.87 DD411 pKa = 3.5 IVTPGGTVSITGRR424 pKa = 11.84 VINQVVAQVANLEE437 pKa = 4.06 RR438 pKa = 11.84 DD439 pKa = 3.16 NNYY442 pKa = 9.78 YY443 pKa = 10.66 SRR445 pKa = 11.84 VRR447 pKa = 11.84 AFTLSRR453 pKa = 11.84 LIAEE457 pKa = 4.68 GSDD460 pKa = 2.89 RR461 pKa = 11.84 SLVKK465 pKa = 9.92 WVVEE469 pKa = 4.22 VVMAMADD476 pKa = 3.32 SFAYY480 pKa = 10.04 EE481 pKa = 4.26 LVRR484 pKa = 11.84 HH485 pKa = 6.52 CNTGMQPDD493 pKa = 4.09 MLTVVDD499 pKa = 3.92 HH500 pKa = 5.48 TRR502 pKa = 11.84 EE503 pKa = 3.76 RR504 pKa = 11.84 LRR506 pKa = 11.84 RR507 pKa = 11.84 TLITTLRR514 pKa = 11.84 SFYY517 pKa = 10.56 TSTWLEE523 pKa = 4.0 GLCARR528 pKa = 11.84 RR529 pKa = 11.84 PIARR533 pKa = 11.84 KK534 pKa = 7.29 LTPWVFKK541 pKa = 10.13 DD542 pKa = 2.98 IRR544 pKa = 11.84 MPTYY548 pKa = 10.19 EE549 pKa = 4.35 VYY551 pKa = 9.85 TPPRR555 pKa = 11.84 HH556 pKa = 6.04 RR557 pKa = 11.84 EE558 pKa = 3.5 EE559 pKa = 4.37 RR560 pKa = 11.84 MPAAAEE566 pKa = 3.88 NPFPVRR572 pKa = 11.84 RR573 pKa = 11.84 PPVNAGPDD581 pKa = 3.59 EE582 pKa = 4.29 RR583 pKa = 11.84 RR584 pKa = 11.84 GDD586 pKa = 4.26 LPGQHH591 pKa = 5.16 QRR593 pKa = 11.84 QRR595 pKa = 11.84 QRR597 pKa = 11.84 EE598 pKa = 4.0 RR599 pKa = 11.84 GIEE602 pKa = 4.07 GASTDD607 pKa = 3.2 SGRR610 pKa = 11.84 ASSADD615 pKa = 3.43 SSSDD619 pKa = 4.74 DD620 pKa = 4.17 GDD622 pKa = 4.41 SDD624 pKa = 5.79 DD625 pKa = 5.9 DD626 pKa = 4.35 PLHH629 pKa = 7.11 AIPPPPPPYY638 pKa = 9.85 RR639 pKa = 11.84 GPAGDD644 pKa = 5.1 EE645 pKa = 3.72 ITALIVPPPPSFARR659 pKa = 11.84 AGSPEE664 pKa = 3.49 HH665 pKa = 6.33 HH666 pKa = 6.64 RR667 pKa = 11.84 PVEE670 pKa = 3.77 NDD672 pKa = 2.49 GRR674 pKa = 11.84 PRR676 pKa = 11.84 RR677 pKa = 11.84 DD678 pKa = 2.96 SGITRR683 pKa = 11.84 SGDD686 pKa = 3.41 SPTRR690 pKa = 11.84 RR691 pKa = 11.84 APRR694 pKa = 11.84 GAGRR698 pKa = 11.84 DD699 pKa = 3.71 DD700 pKa = 4.55 DD701 pKa = 5.14 SGAEE705 pKa = 4.17 TEE707 pKa = 4.25 TSYY710 pKa = 9.78 RR711 pKa = 11.84 TASTTHH717 pKa = 7.57 DD718 pKa = 4.26 DD719 pKa = 3.76 PLSDD723 pKa = 3.84 VEE725 pKa = 4.97 IVTHH729 pKa = 5.71 PMRR732 pKa = 11.84 LGAVRR737 pKa = 11.84 GPRR740 pKa = 11.84 VLLEE744 pKa = 4.5 CGTKK748 pKa = 9.93 RR749 pKa = 11.84 GRR751 pKa = 11.84 TILRR755 pKa = 11.84 LHH757 pKa = 7.35 DD758 pKa = 4.07 SSQDD762 pKa = 3.43 LHH764 pKa = 7.98 SEE766 pKa = 4.29 PPSTTLPRR774 pKa = 11.84 RR775 pKa = 11.84 PSRR778 pKa = 11.84 AQLNEE783 pKa = 3.41 VLEE786 pKa = 4.28 RR787 pKa = 11.84 VEE789 pKa = 4.3 EE790 pKa = 4.57 GVGRR794 pKa = 11.84 PTNHH798 pKa = 5.89 TPQRR802 pKa = 11.84 AEE804 pKa = 3.63 HH805 pKa = 6.69 LFYY808 pKa = 10.19 QTLRR812 pKa = 11.84 NLGLLPTGQSPAQPRR827 pKa = 11.84 RR828 pKa = 11.84 GVPPNQRR835 pKa = 11.84 DD836 pKa = 3.72 GVVGGSEE843 pKa = 4.45 RR844 pKa = 11.84 ADD846 pKa = 3.5 EE847 pKa = 4.29 LPKK850 pKa = 10.19 VDD852 pKa = 4.68 KK853 pKa = 11.0 ALSHH857 pKa = 6.8 PPAGRR862 pKa = 11.84 AGRR865 pKa = 11.84 GTGRR869 pKa = 11.84 GRR871 pKa = 11.84 GPRR874 pKa = 11.84 PGARR878 pKa = 11.84 GRR880 pKa = 11.84 KK881 pKa = 7.72 NN882 pKa = 2.93
Molecular weight: 99.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.819
IPC2_protein 8.053
IPC_protein 7.936
Toseland 7.732
ProMoST 8.434
Dawson 8.609
Bjellqvist 8.873
Wikipedia 8.595
Rodwell 8.639
Grimsley 7.819
Solomon 8.682
Lehninger 8.668
Nozaki 8.946
DTASelect 8.653
Thurlkill 8.682
EMBOSS 8.785
Sillero 8.96
Patrickios 4.596
IPC_peptide 8.668
IPC2_peptide 7.922
IPC2.peptide.svr19 7.965
Protein with the highest isoelectric point:
>tr|A0A1L3KGF4|A0A1L3KGF4_9VIRU Chropara_Vmeth domain-containing protein OS=Hubei odonate virus 12 OX=1922993 PE=4 SV=1
MM1 pKa = 7.93 RR2 pKa = 11.84 CTLPPVTGRR11 pKa = 11.84 SACRR15 pKa = 11.84 QLLRR19 pKa = 11.84 THH21 pKa = 6.42 FQCGARR27 pKa = 11.84 QLMPALTSAEE37 pKa = 4.11 VTFPANTRR45 pKa = 11.84 DD46 pKa = 3.58 NASASVALRR55 pKa = 11.84 VLRR58 pKa = 11.84 PTLAVHH64 pKa = 6.99 PLLTVPPMTGTAMMIRR80 pKa = 11.84 CMPSPPPPHH89 pKa = 7.58 LIEE92 pKa = 4.94 DD93 pKa = 3.85 QPGTRR98 pKa = 11.84 SQHH101 pKa = 5.32 LSSRR105 pKa = 11.84 HH106 pKa = 5.68 HH107 pKa = 6.8 PPSPALGVRR116 pKa = 11.84 NTTDD120 pKa = 3.37 QLRR123 pKa = 11.84 MMGVRR128 pKa = 11.84 VEE130 pKa = 4.39 TVEE133 pKa = 4.1 SLEE136 pKa = 4.17 AGIARR141 pKa = 11.84 LDD143 pKa = 3.51 VLRR146 pKa = 11.84 EE147 pKa = 3.96 EE148 pKa = 4.67 LEE150 pKa = 4.16 GMTTAGRR157 pKa = 11.84 RR158 pKa = 11.84 RR159 pKa = 11.84 KK160 pKa = 10.06 RR161 pKa = 11.84 PTALLVPPMTIPYY174 pKa = 7.18 RR175 pKa = 11.84 TSRR178 pKa = 11.84 SSRR181 pKa = 11.84 IRR183 pKa = 11.84 CDD185 pKa = 3.23 LAPFADD191 pKa = 5.71 PEE193 pKa = 4.68 SCWNVEE199 pKa = 4.11 PNGDD203 pKa = 4.12 GPYY206 pKa = 10.12 SDD208 pKa = 3.96 CTIVRR213 pKa = 11.84 KK214 pKa = 9.97 IYY216 pKa = 9.41 TRR218 pKa = 11.84 SRR220 pKa = 11.84 RR221 pKa = 11.84 QQPFQDD227 pKa = 3.75 VQVVPSSTKK236 pKa = 9.8 CWRR239 pKa = 11.84 EE240 pKa = 3.59 WKK242 pKa = 10.45 KK243 pKa = 10.97 EE244 pKa = 3.79 WGDD247 pKa = 3.75 LPTIPHH253 pKa = 6.29 SEE255 pKa = 3.81 QSICFTKK262 pKa = 10.48 RR263 pKa = 11.84 SVISACSRR271 pKa = 11.84 RR272 pKa = 11.84 DD273 pKa = 3.29 SPLRR277 pKa = 11.84 NLVAEE282 pKa = 4.48 FLQTKK287 pKa = 8.23 EE288 pKa = 4.21 MEE290 pKa = 3.95 WLEE293 pKa = 4.15 AVSEE297 pKa = 4.08 PMNFQRR303 pKa = 11.84 WIKK306 pKa = 9.95 RR307 pKa = 11.84 YY308 pKa = 7.8 PIHH311 pKa = 7.1 RR312 pKa = 11.84 RR313 pKa = 11.84 VEE315 pKa = 4.05 LEE317 pKa = 3.61 EE318 pKa = 4.31 ARR320 pKa = 11.84 AEE322 pKa = 4.15 VEE324 pKa = 3.84 ARR326 pKa = 11.84 GLEE329 pKa = 4.06 RR330 pKa = 11.84 GDD332 pKa = 4.1 AKK334 pKa = 10.48 ISCFIKK340 pKa = 10.57 RR341 pKa = 11.84 EE342 pKa = 4.22 ATVKK346 pKa = 10.52 RR347 pKa = 11.84 VDD349 pKa = 3.74 PRR351 pKa = 11.84 NISPRR356 pKa = 11.84 TDD358 pKa = 2.84 QFLATLGPYY367 pKa = 8.75 IAAMEE372 pKa = 4.44 AKK374 pKa = 9.99 ACRR377 pKa = 11.84 APFMVKK383 pKa = 9.13 GHH385 pKa = 6.1 NLRR388 pKa = 11.84 GIQRR392 pKa = 11.84 KK393 pKa = 9.39 LGWLADD399 pKa = 3.63 YY400 pKa = 10.7 QSYY403 pKa = 10.28 VEE405 pKa = 4.14 VDD407 pKa = 4.14 FSRR410 pKa = 11.84 FDD412 pKa = 3.32 MTLTRR417 pKa = 11.84 HH418 pKa = 5.37 ILEE421 pKa = 4.2 IVEE424 pKa = 3.95 NTMLTKK430 pKa = 10.67 AFPKK434 pKa = 9.76 EE435 pKa = 3.93 LHH437 pKa = 6.75 PEE439 pKa = 3.66 LHH441 pKa = 6.57 IALKK445 pKa = 10.23 LARR448 pKa = 11.84 SPAGRR453 pKa = 11.84 YY454 pKa = 8.71 RR455 pKa = 11.84 CGVSYY460 pKa = 9.49 STEE463 pKa = 3.89 GTRR466 pKa = 11.84 CSGDD470 pKa = 2.75 AHH472 pKa = 6.1 TSFGNTLLNRR482 pKa = 11.84 FFTWLCLRR490 pKa = 11.84 KK491 pKa = 9.79 LPSSSWRR498 pKa = 11.84 SVHH501 pKa = 6.6 EE502 pKa = 4.52 GDD504 pKa = 5.04 DD505 pKa = 3.59 GLIAVSSRR513 pKa = 11.84 WVNQVLYY520 pKa = 10.61 NLSCLGCLGLEE531 pKa = 4.44 PKK533 pKa = 10.76 LKK535 pKa = 9.81 VTDD538 pKa = 4.07 NLHH541 pKa = 6.05 EE542 pKa = 4.14 ATFCGRR548 pKa = 11.84 NFHH551 pKa = 6.29 STTDD555 pKa = 4.49 GIISTCDD562 pKa = 3.06 VPRR565 pKa = 11.84 ALQKK569 pKa = 10.94 YY570 pKa = 7.02 HH571 pKa = 5.84 TTTSDD576 pKa = 3.23 GKK578 pKa = 10.02 PDD580 pKa = 3.37 ILLLCKK586 pKa = 10.19 SVSYY590 pKa = 10.27 LYY592 pKa = 10.98 SDD594 pKa = 3.38 GHH596 pKa = 5.53 TPGVGLLASSLVQILIKK613 pKa = 10.33 RR614 pKa = 11.84 VQCQKK619 pKa = 10.36 QFEE622 pKa = 4.58 KK623 pKa = 10.56 TMHH626 pKa = 6.26 RR627 pKa = 11.84 VLKK630 pKa = 9.82 EE631 pKa = 3.65 RR632 pKa = 11.84 YY633 pKa = 9.04 AADD636 pKa = 3.31 VLIGKK641 pKa = 8.86 KK642 pKa = 9.96 NKK644 pKa = 10.03 YY645 pKa = 10.37 FMGDD649 pKa = 3.18 VLEE652 pKa = 5.25 KK653 pKa = 10.78 ARR655 pKa = 11.84 AWDD658 pKa = 3.63 PRR660 pKa = 11.84 MVSPTVEE667 pKa = 3.81 ANVAHH672 pKa = 6.9 TYY674 pKa = 9.99 GIPLQPFLRR683 pKa = 11.84 SVSQFSSWVKK693 pKa = 10.23 FGHH696 pKa = 5.82 VPAEE700 pKa = 4.17 VEE702 pKa = 4.31 VLQLEE707 pKa = 4.4 TLEE710 pKa = 5.08 DD711 pKa = 3.47 KK712 pKa = 11.17 AGTVVYY718 pKa = 8.9 TT719 pKa = 4.03
Molecular weight: 81.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.771
IPC2_protein 8.551
IPC_protein 8.419
Toseland 8.887
ProMoST 8.99
Dawson 9.341
Bjellqvist 9.355
Wikipedia 9.458
Rodwell 9.487
Grimsley 9.238
Solomon 9.385
Lehninger 9.355
Nozaki 9.575
DTASelect 9.18
Thurlkill 9.297
EMBOSS 9.502
Sillero 9.502
Patrickios 4.876
IPC_peptide 9.385
IPC2_peptide 8.375
IPC2.peptide.svr19 7.802
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1601
719
882
800.5
90.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.183 ± 0.22
2.061 ± 0.515
5.309 ± 0.681
6.371 ± 0.234
3.061 ± 0.083
6.746 ± 0.874
3.123 ± 0.121
3.623 ± 0.163
3.498 ± 0.654
7.995 ± 1.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.124 ± 0.311
2.811 ± 0.184
7.121 ± 0.183
3.061 ± 0.249
10.369 ± 0.795
6.996 ± 0.308
6.496 ± 0.441
7.37 ± 0.083
1.686 ± 0.01
2.998 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here