Turnip curly top virus (isolate Turnip/South Africa/B11/2006)
Average proteome isoelectric point is 7.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D8WZ27|D8WZ27_TCTVB Capsid protein OS=Turnip curly top virus (isolate Turnip/South Africa/B11/2006) OX=1559365 PE=3 SV=1
MM1 pKa = 7.47 IWRR4 pKa = 11.84 IAKK7 pKa = 9.52 DD8 pKa = 3.65 DD9 pKa = 3.4 KK10 pKa = 10.67 RR11 pKa = 11.84 VRR13 pKa = 11.84 AFRR16 pKa = 11.84 YY17 pKa = 8.4 KK18 pKa = 10.17 KK19 pKa = 9.85 RR20 pKa = 11.84 RR21 pKa = 11.84 FYY23 pKa = 11.29 SLSEE27 pKa = 3.99 YY28 pKa = 10.94 LLAPLATHH36 pKa = 6.96 LRR38 pKa = 11.84 LARR41 pKa = 11.84 ILPEE45 pKa = 4.29 KK46 pKa = 9.8 CWSVVPAYY54 pKa = 9.53 TDD56 pKa = 3.16 EE57 pKa = 4.98 AYY59 pKa = 11.03 SFLFMLKK66 pKa = 10.15 EE67 pKa = 3.96 EE68 pKa = 4.39 TRR70 pKa = 11.84 TPFQILKK77 pKa = 10.45 DD78 pKa = 3.52 EE79 pKa = 4.46 WNVEE83 pKa = 4.27 FKK85 pKa = 10.46 EE86 pKa = 4.41 AEE88 pKa = 4.11 VALSRR93 pKa = 11.84 LRR95 pKa = 11.84 LSTEE99 pKa = 3.8 DD100 pKa = 3.22 TGEE103 pKa = 3.96 EE104 pKa = 4.08 TGGEE108 pKa = 4.14 EE109 pKa = 5.2 SPVQQSSPVQSSSCEE124 pKa = 3.92 TT125 pKa = 3.49
Molecular weight: 14.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.632
IPC2_protein 5.868
IPC_protein 5.804
Toseland 5.855
ProMoST 5.957
Dawson 5.881
Bjellqvist 5.931
Wikipedia 5.779
Rodwell 5.83
Grimsley 5.842
Solomon 5.881
Lehninger 5.842
Nozaki 6.033
DTASelect 6.16
Thurlkill 6.046
EMBOSS 5.982
Sillero 6.148
Patrickios 4.406
IPC_peptide 5.893
IPC2_peptide 6.109
IPC2.peptide.svr19 6.335
Protein with the highest isoelectric point:
>tr|D8WZ29|D8WZ29_TCTVB C2 OS=Turnip curly top virus (isolate Turnip/South Africa/B11/2006) OX=1559365 PE=3 SV=1
MM1 pKa = 7.39 SVVRR5 pKa = 11.84 DD6 pKa = 3.8 LRR8 pKa = 11.84 TGEE11 pKa = 4.83 PITHH15 pKa = 6.2 RR16 pKa = 11.84 QSTIGTYY23 pKa = 9.16 IHH25 pKa = 5.99 QVPNPFHH32 pKa = 6.97 LKK34 pKa = 10.29 LISWDD39 pKa = 3.4 HH40 pKa = 5.56 QEE42 pKa = 4.21 NGFFNIRR49 pKa = 11.84 LQLRR53 pKa = 11.84 FNYY56 pKa = 10.1 NLRR59 pKa = 11.84 KK60 pKa = 9.6 QLGLHH65 pKa = 5.5 MAWIEE70 pKa = 3.88 FTVLGRR76 pKa = 11.84 HH77 pKa = 6.14 RR78 pKa = 11.84 SLTGHH83 pKa = 7.2 RR84 pKa = 11.84 FTTIFLKK91 pKa = 10.8 RR92 pKa = 11.84 LLFYY96 pKa = 10.99 LNNLGIISLNLVINGISHH114 pKa = 6.86 VLFKK118 pKa = 10.82 DD119 pKa = 4.25 FKK121 pKa = 10.57 FVEE124 pKa = 4.44 SVINQEE130 pKa = 3.57 YY131 pKa = 8.8 RR132 pKa = 11.84 VAMKK136 pKa = 10.76 EE137 pKa = 3.68 EE138 pKa = 5.1 FYY140 pKa = 11.56
Molecular weight: 16.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.24
IPC2_protein 9.545
IPC_protein 9.984
Toseland 10.145
ProMoST 9.926
Dawson 10.365
Bjellqvist 10.058
Wikipedia 10.57
Rodwell 10.643
Grimsley 10.452
Solomon 10.409
Lehninger 10.365
Nozaki 10.116
DTASelect 10.058
Thurlkill 10.204
EMBOSS 10.555
Sillero 10.277
Patrickios 10.292
IPC_peptide 10.409
IPC2_peptide 8.741
IPC2.peptide.svr19 8.691
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1138
85
371
189.7
21.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.482 ± 0.977
1.318 ± 0.254
4.833 ± 0.826
5.712 ± 1.029
4.833 ± 0.689
5.712 ± 0.611
3.427 ± 0.714
6.942 ± 1.068
6.678 ± 0.876
7.645 ± 1.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.373 ± 0.786
5.097 ± 0.682
5.624 ± 0.649
3.954 ± 0.428
6.415 ± 0.93
8.084 ± 1.364
6.151 ± 0.862
4.921 ± 0.504
1.933 ± 0.408
3.866 ± 0.715
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here