Amino acid dipepetide frequency for Cnuibacter physcomitrellae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.608AlaAla: 20.608 ± 0.199	0.643AlaCys: 0.643 ± 0.028	8.352AlaAsp: 8.352 ± 0.089	7.886AlaGlu: 7.886 ± 0.095	3.85AlaPhe: 3.85 ± 0.055	12.299AlaGly: 12.299 ± 0.106	2.304AlaHis: 2.304 ± 0.042	5.464AlaIle: 5.464 ± 0.069	2.247AlaLys: 2.247 ± 0.047	14.32AlaLeu: 14.32 ± 0.144	2.492AlaMet: 2.492 ± 0.043	2.026AlaAsn: 2.026 ± 0.044	6.688AlaPro: 6.688 ± 0.089	3.674AlaGln: 3.674 ± 0.062	9.093AlaArg: 9.093 ± 0.116	8.199AlaSer: 8.199 ± 0.098	7.545AlaThr: 7.545 ± 0.089	12.153AlaVal: 12.153 ± 0.117	1.737AlaTrp: 1.737 ± 0.041	2.357AlaTyr: 2.357 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.65CysAla: 0.65 ± 0.023	0.054CysCys: 0.054 ± 0.007	0.277CysAsp: 0.277 ± 0.014	0.213CysGlu: 0.213 ± 0.013	0.168CysPhe: 0.168 ± 0.012	0.549CysGly: 0.549 ± 0.023	0.088CysHis: 0.088 ± 0.008	0.177CysIle: 0.177 ± 0.011	0.045CysLys: 0.045 ± 0.005	0.467CysLeu: 0.467 ± 0.021	0.066CysMet: 0.066 ± 0.008	0.091CysAsn: 0.091 ± 0.009	0.271CysPro: 0.271 ± 0.015	0.116CysGln: 0.116 ± 0.01	0.332CysArg: 0.332 ± 0.016	0.369CysSer: 0.369 ± 0.018	0.311CysThr: 0.311 ± 0.017	0.45CysVal: 0.45 ± 0.018	0.067CysTrp: 0.067 ± 0.008	0.091CysTyr: 0.091 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.555AspAla: 8.555 ± 0.095	0.263AspCys: 0.263 ± 0.016	4.733AspAsp: 4.733 ± 0.083	4.42AspGlu: 4.42 ± 0.059	1.733AspPhe: 1.733 ± 0.041	6.21AspGly: 6.21 ± 0.08	1.188AspHis: 1.188 ± 0.034	2.201AspIle: 2.201 ± 0.039	0.782AspLys: 0.782 ± 0.03	6.463AspLeu: 6.463 ± 0.077	0.808AspMet: 0.808 ± 0.029	0.852AspAsn: 0.852 ± 0.029	4.607AspPro: 4.607 ± 0.064	1.651AspGln: 1.651 ± 0.044	4.77AspArg: 4.77 ± 0.066	3.007AspSer: 3.007 ± 0.05	3.007AspThr: 3.007 ± 0.054	5.771AspVal: 5.771 ± 0.065	0.956AspTrp: 0.956 ± 0.029	1.298AspTyr: 1.298 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	6.933GluAla: 6.933 ± 0.086	0.219GluCys: 0.219 ± 0.014	2.549GluAsp: 2.549 ± 0.043	3.035GluGlu: 3.035 ± 0.058	1.582GluPhe: 1.582 ± 0.041	4.304GluGly: 4.304 ± 0.061	1.463GluHis: 1.463 ± 0.038	2.536GluIle: 2.536 ± 0.049	1.138GluLys: 1.138 ± 0.033	6.359GluLeu: 6.359 ± 0.084	0.847GluMet: 0.847 ± 0.028	1.151GluAsn: 1.151 ± 0.031	2.996GluPro: 2.996 ± 0.057	2.089GluGln: 2.089 ± 0.043	5.163GluArg: 5.163 ± 0.082	3.157GluSer: 3.157 ± 0.051	3.067GluThr: 3.067 ± 0.053	4.607GluVal: 4.607 ± 0.075	0.834GluTrp: 0.834 ± 0.024	1.153GluTyr: 1.153 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.971PheAla: 3.971 ± 0.064	0.173PheCys: 0.173 ± 0.013	2.313PheAsp: 2.313 ± 0.045	1.642PheGlu: 1.642 ± 0.038	0.997PhePhe: 0.997 ± 0.035	3.4PheGly: 3.4 ± 0.062	0.536PheHis: 0.536 ± 0.022	1.086PheIle: 1.086 ± 0.031	0.428PheLys: 0.428 ± 0.02	2.786PheLeu: 2.786 ± 0.054	0.395PheMet: 0.395 ± 0.018	0.572PheAsn: 0.572 ± 0.022	1.431PhePro: 1.431 ± 0.033	0.745PheGln: 0.745 ± 0.026	1.768PheArg: 1.768 ± 0.039	1.801PheSer: 1.801 ± 0.039	2.104PheThr: 2.104 ± 0.05	2.812PheVal: 2.812 ± 0.052	0.478PheTrp: 0.478 ± 0.02	0.642PheTyr: 0.642 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	11.086GlyAla: 11.086 ± 0.112	0.593GlyCys: 0.593 ± 0.023	5.209GlyAsp: 5.209 ± 0.077	4.768GlyGlu: 4.768 ± 0.074	3.236GlyPhe: 3.236 ± 0.055	7.954GlyGly: 7.954 ± 0.096	1.739GlyHis: 1.739 ± 0.042	4.564GlyIle: 4.564 ± 0.062	1.783GlyLys: 1.783 ± 0.041	9.062GlyLeu: 9.062 ± 0.103	1.819GlyMet: 1.819 ± 0.04	1.548GlyAsn: 1.548 ± 0.041	4.225GlyPro: 4.225 ± 0.062	2.431GlyGln: 2.431 ± 0.053	6.751GlyArg: 6.751 ± 0.089	6.394GlySer: 6.394 ± 0.077	5.709GlyThr: 5.709 ± 0.112	8.358GlyVal: 8.358 ± 0.086	1.645GlyTrp: 1.645 ± 0.038	2.325GlyTyr: 2.325 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.119HisAla: 2.119 ± 0.04	0.105HisCys: 0.105 ± 0.008	1.328HisAsp: 1.328 ± 0.035	1.082HisGlu: 1.082 ± 0.032	0.489HisPhe: 0.489 ± 0.02	1.84HisGly: 1.84 ± 0.041	0.525HisHis: 0.525 ± 0.023	0.642HisIle: 0.642 ± 0.022	0.216HisLys: 0.216 ± 0.014	1.929HisLeu: 1.929 ± 0.041	0.259HisMet: 0.259 ± 0.015	0.288HisAsn: 0.288 ± 0.016	1.624HisPro: 1.624 ± 0.044	0.466HisGln: 0.466 ± 0.02	1.558HisArg: 1.558 ± 0.034	1.019HisSer: 1.019 ± 0.03	0.934HisThr: 0.934 ± 0.025	1.586HisVal: 1.586 ± 0.041	0.284HisTrp: 0.284 ± 0.016	0.431HisTyr: 0.431 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.576IleAla: 6.576 ± 0.082	0.208IleCys: 0.208 ± 0.012	3.24IleAsp: 3.24 ± 0.058	2.624IleGlu: 2.624 ± 0.044	0.915IlePhe: 0.915 ± 0.031	4.443IleGly: 4.443 ± 0.075	0.618IleHis: 0.618 ± 0.025	1.645IleIle: 1.645 ± 0.05	0.695IleLys: 0.695 ± 0.027	3.764IleLeu: 3.764 ± 0.064	0.533IleMet: 0.533 ± 0.019	0.813IleAsn: 0.813 ± 0.024	2.41IlePro: 2.41 ± 0.045	1.0IleGln: 1.0 ± 0.029	2.769IleArg: 2.769 ± 0.048	2.247IleSer: 2.247 ± 0.046	2.829IleThr: 2.829 ± 0.055	4.527IleVal: 4.527 ± 0.061	0.481IleTrp: 0.481 ± 0.023	0.659IleTyr: 0.659 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	2.201LysAla: 2.201 ± 0.052	0.053LysCys: 0.053 ± 0.006	1.054LysAsp: 1.054 ± 0.027	0.877LysGlu: 0.877 ± 0.031	0.372LysPhe: 0.372 ± 0.018	1.411LysGly: 1.411 ± 0.039	0.362LysHis: 0.362 ± 0.015	0.703LysIle: 0.703 ± 0.026	0.54LysLys: 0.54 ± 0.024	1.578LysLeu: 1.578 ± 0.038	0.292LysMet: 0.292 ± 0.017	0.439LysAsn: 0.439 ± 0.017	1.02LysPro: 1.02 ± 0.025	0.61LysGln: 0.61 ± 0.023	1.378LysArg: 1.378 ± 0.037	0.953LysSer: 0.953 ± 0.028	1.149LysThr: 1.149 ± 0.033	1.417LysVal: 1.417 ± 0.04	0.192LysTrp: 0.192 ± 0.013	0.362LysTyr: 0.362 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.07LeuAla: 15.07 ± 0.143	0.516LeuCys: 0.516 ± 0.019	7.009LeuAsp: 7.009 ± 0.087	5.405LeuGlu: 5.405 ± 0.074	2.915LeuPhe: 2.915 ± 0.061	9.58LeuGly: 9.58 ± 0.096	1.779LeuHis: 1.779 ± 0.04	4.142LeuIle: 4.142 ± 0.064	1.569LeuLys: 1.569 ± 0.037	10.516LeuLeu: 10.516 ± 0.139	1.445LeuMet: 1.445 ± 0.039	1.579LeuAsn: 1.579 ± 0.036	5.643LeuPro: 5.643 ± 0.071	2.511LeuGln: 2.511 ± 0.045	7.359LeuArg: 7.359 ± 0.088	6.238LeuSer: 6.238 ± 0.065	6.345LeuThr: 6.345 ± 0.081	9.926LeuVal: 9.926 ± 0.102	1.218LeuTrp: 1.218 ± 0.033	1.656LeuTyr: 1.656 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.058MetAla: 2.058 ± 0.044	0.087MetCys: 0.087 ± 0.007	0.738MetAsp: 0.738 ± 0.021	0.59MetGlu: 0.59 ± 0.021	0.428MetPhe: 0.428 ± 0.017	1.219MetGly: 1.219 ± 0.03	0.32MetHis: 0.32 ± 0.016	0.765MetIle: 0.765 ± 0.025	0.325MetLys: 0.325 ± 0.015	1.877MetLeu: 1.877 ± 0.042	0.281MetMet: 0.281 ± 0.017	0.404MetAsn: 0.404 ± 0.022	1.062MetPro: 1.062 ± 0.028	0.475MetGln: 0.475 ± 0.017	1.385MetArg: 1.385 ± 0.033	1.402MetSer: 1.402 ± 0.036	1.629MetThr: 1.629 ± 0.03	1.286MetVal: 1.286 ± 0.034	0.2MetTrp: 0.2 ± 0.012	0.262MetTyr: 0.262 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.271AsnAla: 2.271 ± 0.05	0.089AsnCys: 0.089 ± 0.009	1.073AsnAsp: 1.073 ± 0.034	0.821AsnGlu: 0.821 ± 0.031	0.507AsnPhe: 0.507 ± 0.02	1.847AsnGly: 1.847 ± 0.05	0.328AsnHis: 0.328 ± 0.016	0.722AsnIle: 0.722 ± 0.022	0.335AsnLys: 0.335 ± 0.018	1.742AsnLeu: 1.742 ± 0.038	0.248AsnMet: 0.248 ± 0.014	0.397AsnAsn: 0.397 ± 0.017	1.456AsnPro: 1.456 ± 0.033	0.519AsnGln: 0.519 ± 0.021	1.106AsnArg: 1.106 ± 0.028	0.856AsnSer: 0.856 ± 0.031	1.022AsnThr: 1.022 ± 0.036	1.564AsnVal: 1.564 ± 0.037	0.249AsnTrp: 0.249 ± 0.015	0.412AsnTyr: 0.412 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	7.647ProAla: 7.647 ± 0.088	0.198ProCys: 0.198 ± 0.014	4.171ProAsp: 4.171 ± 0.064	3.737ProGlu: 3.737 ± 0.063	1.736ProPhe: 1.736 ± 0.036	5.382ProGly: 5.382 ± 0.067	1.107ProHis: 1.107 ± 0.031	2.138ProIle: 2.138 ± 0.037	0.866ProLys: 0.866 ± 0.032	5.146ProLeu: 5.146 ± 0.064	0.847ProMet: 0.847 ± 0.026	0.972ProAsn: 0.972 ± 0.03	2.632ProPro: 2.632 ± 0.065	1.459ProGln: 1.459 ± 0.036	3.576ProArg: 3.576 ± 0.062	3.838ProSer: 3.838 ± 0.068	3.671ProThr: 3.671 ± 0.065	5.049ProVal: 5.049 ± 0.065	0.838ProTrp: 0.838 ± 0.026	1.107ProTyr: 1.107 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.372GlnAla: 3.372 ± 0.068	0.112GlnCys: 0.112 ± 0.009	1.278GlnAsp: 1.278 ± 0.033	1.237GlnGlu: 1.237 ± 0.036	0.775GlnPhe: 0.775 ± 0.028	2.17GlnGly: 2.17 ± 0.044	0.577GlnHis: 0.577 ± 0.025	1.363GlnIle: 1.363 ± 0.033	0.594GlnLys: 0.594 ± 0.024	3.058GlnLeu: 3.058 ± 0.055	0.439GlnMet: 0.439 ± 0.018	0.635GlnAsn: 0.635 ± 0.025	1.512GlnPro: 1.512 ± 0.044	1.187GlnGln: 1.187 ± 0.042	2.215GlnArg: 2.215 ± 0.044	1.532GlnSer: 1.532 ± 0.039	1.599GlnThr: 1.599 ± 0.037	2.211GlnVal: 2.211 ± 0.043	0.428GlnTrp: 0.428 ± 0.017	0.659GlnTyr: 0.659 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	8.734ArgAla: 8.734 ± 0.105	0.284ArgCys: 0.284 ± 0.016	4.258ArgAsp: 4.258 ± 0.06	4.195ArgGlu: 4.195 ± 0.078	2.418ArgPhe: 2.418 ± 0.048	5.358ArgGly: 5.358 ± 0.074	1.47ArgHis: 1.47 ± 0.038	3.729ArgIle: 3.729 ± 0.057	1.163ArgLys: 1.163 ± 0.033	7.58ArgLeu: 7.58 ± 0.105	1.838ArgMet: 1.838 ± 0.032	1.124ArgAsn: 1.124 ± 0.033	4.02ArgPro: 4.02 ± 0.058	1.826ArgGln: 1.826 ± 0.042	7.162ArgArg: 7.162 ± 0.092	4.839ArgSer: 4.839 ± 0.065	4.308ArgThr: 4.308 ± 0.058	5.924ArgVal: 5.924 ± 0.076	1.284ArgTrp: 1.284 ± 0.033	1.551ArgTyr: 1.551 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	8.199SerAla: 8.199 ± 0.097	0.271SerCys: 0.271 ± 0.017	3.73SerAsp: 3.73 ± 0.057	2.852SerGlu: 2.852 ± 0.051	1.992SerPhe: 1.992 ± 0.04	6.561SerGly: 6.561 ± 0.081	1.044SerHis: 1.044 ± 0.028	2.819SerIle: 2.819 ± 0.05	1.037SerLys: 1.037 ± 0.034	5.819SerLeu: 5.819 ± 0.073	1.208SerMet: 1.208 ± 0.035	1.117SerAsn: 1.117 ± 0.035	3.574SerPro: 3.574 ± 0.055	1.432SerGln: 1.432 ± 0.036	4.202SerArg: 4.202 ± 0.052	4.316SerSer: 4.316 ± 0.081	4.249SerThr: 4.249 ± 0.063	5.294SerVal: 5.294 ± 0.069	0.984SerTrp: 0.984 ± 0.029	1.252SerTyr: 1.252 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	8.038ThrAla: 8.038 ± 0.095	0.302ThrCys: 0.302 ± 0.015	3.646ThrAsp: 3.646 ± 0.067	2.932ThrGlu: 2.932 ± 0.05	1.832ThrPhe: 1.832 ± 0.049	6.024ThrGly: 6.024 ± 0.084	1.09ThrHis: 1.09 ± 0.026	2.805ThrIle: 2.805 ± 0.056	1.086ThrLys: 1.086 ± 0.033	6.223ThrLeu: 6.223 ± 0.08	0.975ThrMet: 0.975 ± 0.032	1.102ThrAsn: 1.102 ± 0.038	4.238ThrPro: 4.238 ± 0.064	1.419ThrGln: 1.419 ± 0.034	3.761ThrArg: 3.761 ± 0.049	3.853ThrSer: 3.853 ± 0.058	4.237ThrThr: 4.237 ± 0.077	6.198ThrVal: 6.198 ± 0.097	0.905ThrTrp: 0.905 ± 0.033	1.158ThrTyr: 1.158 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	11.887ValAla: 11.887 ± 0.108	0.479ValCys: 0.479 ± 0.021	6.123ValAsp: 6.123 ± 0.072	5.125ValGlu: 5.125 ± 0.078	2.908ValPhe: 2.908 ± 0.052	7.579ValGly: 7.579 ± 0.088	1.6ValHis: 1.6 ± 0.036	4.188ValIle: 4.188 ± 0.062	1.537ValLys: 1.537 ± 0.039	9.916ValLeu: 9.916 ± 0.106	1.437ValMet: 1.437 ± 0.037	1.681ValAsn: 1.681 ± 0.04	4.922ValPro: 4.922 ± 0.066	2.264ValGln: 2.264 ± 0.039	5.936ValArg: 5.936 ± 0.082	5.608ValSer: 5.608 ± 0.065	5.985ValThr: 5.985 ± 0.095	9.744ValVal: 9.744 ± 0.115	1.154ValTrp: 1.154 ± 0.031	1.635ValTyr: 1.635 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.596TrpAla: 1.596 ± 0.038	0.081TrpCys: 0.081 ± 0.009	0.78TrpAsp: 0.78 ± 0.026	0.61TrpGlu: 0.61 ± 0.019	0.571TrpPhe: 0.571 ± 0.021	1.07TrpGly: 1.07 ± 0.025	0.277TrpHis: 0.277 ± 0.014	0.718TrpIle: 0.718 ± 0.023	0.286TrpLys: 0.286 ± 0.014	1.687TrpLeu: 1.687 ± 0.037	0.309TrpMet: 0.309 ± 0.016	0.391TrpAsn: 0.391 ± 0.018	0.691TrpPro: 0.691 ± 0.023	0.51TrpGln: 0.51 ± 0.019	1.214TrpArg: 1.214 ± 0.034	1.054TrpSer: 1.054 ± 0.026	0.955TrpThr: 0.955 ± 0.03	1.128TrpVal: 1.128 ± 0.032	0.348TrpTrp: 0.348 ± 0.022	0.311TrpTyr: 0.311 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.367TyrAla: 2.367 ± 0.046	0.125TyrCys: 0.125 ± 0.011	1.413TyrAsp: 1.413 ± 0.038	1.132TyrGlu: 1.132 ± 0.033	0.652TyrPhe: 0.652 ± 0.025	2.0TyrGly: 2.0 ± 0.042	0.309TyrHis: 0.309 ± 0.017	0.592TyrIle: 0.592 ± 0.021	0.292TyrLys: 0.292 ± 0.015	2.13TyrLeu: 2.13 ± 0.044	0.255TyrMet: 0.255 ± 0.015	0.38TyrAsn: 0.38 ± 0.017	1.094TyrPro: 1.094 ± 0.03	0.568TyrGln: 0.568 ± 0.022	1.568TyrArg: 1.568 ± 0.038	1.268TyrSer: 1.268 ± 0.029	1.17TyrThr: 1.17 ± 0.032	1.702TyrVal: 1.702 ± 0.036	0.303TyrTrp: 0.303 ± 0.015	0.485TyrTyr: 0.485 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4009 proteins (1285964 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---