Sweet potato leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9PED5|J9PED5_9GEMI Replication-associated protein OS=Sweet potato leaf curl virus OX=100755 PE=3 SV=1
MM1 pKa = 7.68LFQSWSPSMADD12 pKa = 2.69MWDD15 pKa = 3.63PLQNPLPDD23 pKa = 3.26TLYY26 pKa = 11.06GFRR29 pKa = 11.84CMLSVKK35 pKa = 9.89YY36 pKa = 10.19LQSILKK42 pKa = 9.68KK43 pKa = 10.31YY44 pKa = 10.47EE45 pKa = 4.14PGTLGFEE52 pKa = 4.51LCSEE56 pKa = 5.38LIRR59 pKa = 11.84IFRR62 pKa = 11.84VRR64 pKa = 11.84QYY66 pKa = 11.53DD67 pKa = 3.6RR68 pKa = 11.84ANARR72 pKa = 11.84YY73 pKa = 9.98AEE75 pKa = 4.24ISSVWGEE82 pKa = 4.06TGKK85 pKa = 8.61TEE87 pKa = 4.27AEE89 pKa = 3.93LRR91 pKa = 11.84DD92 pKa = 4.14SYY94 pKa = 11.36RR95 pKa = 11.84ALHH98 pKa = 6.22WEE100 pKa = 4.38CCPNCCPKK108 pKa = 10.53LCPGFKK114 pKa = 9.88RR115 pKa = 11.84RR116 pKa = 11.84PDD118 pKa = 3.47EE119 pKa = 4.28EE120 pKa = 4.75KK121 pKa = 10.81EE122 pKa = 4.18GG123 pKa = 3.68

Molecular weight: 14.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9PEW5|J9PEW5_9GEMI Replication enhancer OS=Sweet potato leaf curl virus OX=100755 PE=3 SV=1
MM1 pKa = 7.12TGRR4 pKa = 11.84MRR6 pKa = 11.84VTQRR10 pKa = 11.84FHH12 pKa = 7.35PYY14 pKa = 8.86GGRR17 pKa = 11.84PVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84LNFEE26 pKa = 3.93TAIVPYY32 pKa = 9.22TGNAVPIAARR42 pKa = 11.84SYY44 pKa = 8.64VPVSRR49 pKa = 11.84GVRR52 pKa = 11.84MKK54 pKa = 10.51RR55 pKa = 11.84KK56 pKa = 9.94RR57 pKa = 11.84GDD59 pKa = 4.09RR60 pKa = 11.84IPKK63 pKa = 9.95GCVGPCKK70 pKa = 10.08VQDD73 pKa = 3.86YY74 pKa = 8.72EE75 pKa = 4.55FKK77 pKa = 10.1MDD79 pKa = 3.29VPHH82 pKa = 7.25RR83 pKa = 11.84RR84 pKa = 11.84TFVCYY89 pKa = 10.46SDD91 pKa = 3.63FTRR94 pKa = 11.84GTGLTHH100 pKa = 7.28RR101 pKa = 11.84LGKK104 pKa = 9.73RR105 pKa = 11.84VCVKK109 pKa = 10.91SMGIDD114 pKa = 3.27GKK116 pKa = 11.25VWMDD120 pKa = 3.75DD121 pKa = 3.28NVAKK125 pKa = 10.24RR126 pKa = 11.84DD127 pKa = 3.48HH128 pKa = 6.56TNIITYY134 pKa = 9.02WLIRR138 pKa = 11.84DD139 pKa = 3.65RR140 pKa = 11.84RR141 pKa = 11.84PNKK144 pKa = 10.37DD145 pKa = 3.4PLNFDD150 pKa = 3.79QVFTMYY156 pKa = 11.02DD157 pKa = 3.62NEE159 pKa = 4.13PTTAKK164 pKa = 10.17IRR166 pKa = 11.84MDD168 pKa = 3.48LRR170 pKa = 11.84DD171 pKa = 3.42RR172 pKa = 11.84MQVLKK177 pKa = 10.39KK178 pKa = 10.29FSVTVSGGPYY188 pKa = 8.51NHH190 pKa = 7.45KK191 pKa = 10.06EE192 pKa = 3.61QALVRR197 pKa = 11.84KK198 pKa = 8.79FFKK201 pKa = 10.63GLYY204 pKa = 8.96NHH206 pKa = 5.46VTYY209 pKa = 10.88NHH211 pKa = 6.79KK212 pKa = 10.88EE213 pKa = 3.66EE214 pKa = 4.76ANYY217 pKa = 10.32EE218 pKa = 4.16NQLEE222 pKa = 4.28NALMLYY228 pKa = 9.88SASSHH233 pKa = 6.23ASNPVYY239 pKa = 9.35QTLRR243 pKa = 11.84CRR245 pKa = 11.84VYY247 pKa = 10.7FYY249 pKa = 10.92DD250 pKa = 3.57SHH252 pKa = 7.91NNN254 pKa = 3.3

Molecular weight: 29.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1118

85

364

186.3

21.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.098 ± 0.873

2.952 ± 0.781

5.098 ± 0.381

5.098 ± 0.721

4.114 ± 0.563

6.172 ± 0.259

2.952 ± 0.426

5.009 ± 0.638

5.725 ± 0.785

7.424 ± 1.127

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.604

4.919 ± 0.522

6.44 ± 0.951

4.204 ± 0.649

7.424 ± 1.257

8.05 ± 1.41

5.903 ± 1.127

5.546 ± 1.02

1.878 ± 0.316

3.667 ± 0.683
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski