Aerococcus christensenii
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1496 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I1K5H1|A0A2I1K5H1_9LACT Endonuclease OS=Aerococcus christensenii OX=87541 GN=CYJ27_08045 PE=4 SV=1
MM1 pKa = 7.57 LAINQDD7 pKa = 3.57 LVDD10 pKa = 4.1 EE11 pKa = 4.7 YY12 pKa = 10.72 IARR15 pKa = 11.84 GLKK18 pKa = 10.14 EE19 pKa = 4.22 DD20 pKa = 3.61 VPYY23 pKa = 11.27 EE24 pKa = 4.15 DD25 pKa = 3.99 VTTQAIYY32 pKa = 9.57 MGQQATVQLLAKK44 pKa = 9.69 EE45 pKa = 4.19 SGIICGLAVFEE56 pKa = 6.05 RR57 pKa = 11.84 VFQYY61 pKa = 11.13 LDD63 pKa = 3.55 PQTTCQWLVQEE74 pKa = 4.84 GDD76 pKa = 3.53 AVV78 pKa = 3.44
Molecular weight: 8.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.787
IPC2_protein 3.961
IPC_protein 3.834
Toseland 3.643
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.757
Sillero 3.948
Patrickios 0.693
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|A0A0X8F826|A0A0X8F826_9LACT 50S ribosomal protein L33 OS=Aerococcus christensenii OX=87541 GN=rpmG PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.3 QPNKK9 pKa = 9.14 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 QKK14 pKa = 10.64 KK15 pKa = 8.16 HH16 pKa = 4.35 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 10.03 NGRR28 pKa = 11.84 HH29 pKa = 3.65 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 9.96 GRR39 pKa = 11.84 KK40 pKa = 9.01 RR41 pKa = 11.84 ISAA44 pKa = 3.72
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.45
IPC2_protein 11.169
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.427
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1496
0
1496
463502
23
2350
309.8
34.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.272 ± 0.088
0.853 ± 0.02
5.385 ± 0.058
7.251 ± 0.073
4.202 ± 0.049
6.597 ± 0.062
2.211 ± 0.029
6.921 ± 0.064
7.041 ± 0.081
10.117 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.573 ± 0.032
4.183 ± 0.045
3.578 ± 0.033
4.754 ± 0.059
4.313 ± 0.054
5.979 ± 0.043
5.34 ± 0.053
6.663 ± 0.055
0.911 ± 0.023
3.857 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here