Sphingobacterium daejeonense

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U9JN63|A0A4U9JN63_9SPHI 3-oxoacyl-[acyl-carrier-protein] synthase III OS=Sphingobacterium daejeonense OX=371142 GN=NCTC13534_01583 PE=4 SV=1
MM1 pKa = 7.51ALDD4 pKa = 4.24SYY6 pKa = 11.41RR7 pKa = 11.84CLEE10 pKa = 4.23EE11 pKa = 4.6EE12 pKa = 4.24VLNYY16 pKa = 10.34PEE18 pKa = 5.66DD19 pKa = 4.55GDD21 pKa = 3.94VGSVLGIGFPAQTGGVFSYY40 pKa = 10.67IDD42 pKa = 3.89NIGLDD47 pKa = 3.82EE48 pKa = 5.0FIKK51 pKa = 10.63NCEE54 pKa = 4.15SFSQYY59 pKa = 10.2GDD61 pKa = 2.73EE62 pKa = 4.22WEE64 pKa = 4.36IPEE67 pKa = 4.3SLKK70 pKa = 10.79KK71 pKa = 10.72LRR73 pKa = 11.84DD74 pKa = 3.56EE75 pKa = 5.15GFTFYY80 pKa = 11.17DD81 pKa = 4.0GFISHH86 pKa = 6.9WPP88 pKa = 3.39

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V6JNY8|A0A4V6JNY8_9SPHI Uncharacterized protein OS=Sphingobacterium daejeonense OX=371142 GN=NCTC13534_00691 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.39KK6 pKa = 9.62RR7 pKa = 11.84KK8 pKa = 6.72RR9 pKa = 11.84HH10 pKa = 5.17KK11 pKa = 10.05MATHH15 pKa = 6.02KK16 pKa = 10.34RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight: 3.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5549

0

5549

1347359

29

2666

242.8

27.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.415 ± 0.041

0.767 ± 0.01

5.565 ± 0.026

6.488 ± 0.035

5.071 ± 0.029

6.562 ± 0.034

1.809 ± 0.018

7.695 ± 0.041

7.445 ± 0.035

9.36 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.451 ± 0.017

6.037 ± 0.038

3.597 ± 0.021

3.666 ± 0.02

3.91 ± 0.024

6.634 ± 0.033

5.168 ± 0.033

6.047 ± 0.027

1.192 ± 0.013

4.12 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski