Microviridae sp. ctnrr37

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W727|A0A5Q2W727_9VIRU Scaffold protein OS=Microviridae sp. ctnrr37 OX=2656704 PE=4 SV=1
MM1 pKa = 7.44LRR3 pKa = 11.84GSVPSVSSRR12 pKa = 11.84SQHH15 pKa = 4.63TFSRR19 pKa = 11.84APQAGIPRR27 pKa = 11.84SSFNRR32 pKa = 11.84TFSVKK37 pKa = 7.06TTFNEE42 pKa = 5.29GYY44 pKa = 9.78LVPILADD51 pKa = 3.32EE52 pKa = 4.46MLPGDD57 pKa = 3.88TFSLKK62 pKa = 8.49MHH64 pKa = 6.67AFARR68 pKa = 11.84ISTLLFPIMDD78 pKa = 4.48NLYY81 pKa = 8.88MEE83 pKa = 4.9SFFFFVPNRR92 pKa = 11.84LTWDD96 pKa = 2.84NWEE99 pKa = 4.33NFCQVSTGPTDD110 pKa = 3.33TTEE113 pKa = 3.93YY114 pKa = 10.34TIPQQTGPDD123 pKa = 3.65TMAGGYY129 pKa = 10.49AEE131 pKa = 5.61GSLADD136 pKa = 3.82YY137 pKa = 10.6FGIPTKK143 pKa = 10.55IGGLTPNALHH153 pKa = 6.2FRR155 pKa = 11.84AYY157 pKa = 10.71NLIWNEE163 pKa = 3.75WFRR166 pKa = 11.84SEE168 pKa = 5.07DD169 pKa = 3.33LQAEE173 pKa = 4.62VTVDD177 pKa = 3.32MGDD180 pKa = 3.98GPDD183 pKa = 3.83DD184 pKa = 3.65ATDD187 pKa = 3.59YY188 pKa = 11.64VLLQRR193 pKa = 11.84GKK195 pKa = 10.04RR196 pKa = 11.84YY197 pKa = 10.49DD198 pKa = 3.66YY199 pKa = 8.68FTSCLPWPQKK209 pKa = 11.04GPDD212 pKa = 3.36VLLPLGSSAPVQLISPATDD231 pKa = 2.85TMIGVTGSGNTPVLSQTAIGTNGTGGLQSAPGGNGINIDD270 pKa = 3.93PNGQLIADD278 pKa = 4.39LTDD281 pKa = 3.12ATAATVNQVRR291 pKa = 11.84LAFQTQKK298 pKa = 10.58FYY300 pKa = 11.35EE301 pKa = 4.17KK302 pKa = 9.84QARR305 pKa = 11.84GGTRR309 pKa = 11.84YY310 pKa = 8.49TEE312 pKa = 4.33VIKK315 pKa = 10.96SHH317 pKa = 6.92FDD319 pKa = 3.35VVSPDD324 pKa = 3.11MRR326 pKa = 11.84LQRR329 pKa = 11.84PEE331 pKa = 4.1YY332 pKa = 9.65IGGGSTPVMINPVAQTAPTSGSNALAQLGAYY363 pKa = 7.44GVCAPEE369 pKa = 3.85AHH371 pKa = 6.5GFTYY375 pKa = 10.53SATEE379 pKa = 3.67HH380 pKa = 5.9GVIVGLINVRR390 pKa = 11.84ADD392 pKa = 3.43LNYY395 pKa = 10.4QSGLDD400 pKa = 3.81RR401 pKa = 11.84MWTRR405 pKa = 11.84QVFTDD410 pKa = 3.84YY411 pKa = 9.81YY412 pKa = 9.27WPVFSHH418 pKa = 6.89LGEE421 pKa = 3.99QAVLNQEE428 pKa = 4.66IYY430 pKa = 10.92AQGTSADD437 pKa = 3.65TDD439 pKa = 3.54VFGYY443 pKa = 7.41QEE445 pKa = 3.49RR446 pKa = 11.84HH447 pKa = 4.66AEE449 pKa = 3.95YY450 pKa = 9.88RR451 pKa = 11.84YY452 pKa = 10.22KK453 pKa = 10.72PSLITGLFRR462 pKa = 11.84SNATGTLDD470 pKa = 3.44SWHH473 pKa = 6.89LAQDD477 pKa = 4.29FGSLPEE483 pKa = 4.89LNDD486 pKa = 3.27EE487 pKa = 5.46FIVEE491 pKa = 4.52DD492 pKa = 4.1APMSRR497 pKa = 11.84IKK499 pKa = 10.7AVASSYY505 pKa = 11.59DD506 pKa = 3.43FLFDD510 pKa = 4.12AVFAYY515 pKa = 10.2HH516 pKa = 7.24CARR519 pKa = 11.84PMPVYY524 pKa = 10.31AVPGLIDD531 pKa = 3.33HH532 pKa = 7.15FF533 pKa = 4.9

Molecular weight: 58.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W648|A0A5Q2W648_9VIRU Uncharacterized protein OS=Microviridae sp. ctnrr37 OX=2656704 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.31LWLRR6 pKa = 11.84QNRR9 pKa = 11.84ILNLRR14 pKa = 11.84HH15 pKa = 6.52NILINNLNLILRR27 pKa = 11.84HH28 pKa = 5.77NYY30 pKa = 9.07INNHH34 pKa = 5.33SLSIFRR40 pKa = 11.84IKK42 pKa = 10.05TLVRR46 pKa = 11.84ASKK49 pKa = 10.24HH50 pKa = 4.56FKK52 pKa = 10.26RR53 pKa = 11.84VAKK56 pKa = 9.78RR57 pKa = 11.84LKK59 pKa = 10.4VEE61 pKa = 4.37LSAISSPSSRR71 pKa = 11.84RR72 pKa = 11.84DD73 pKa = 2.92SFAFAKK79 pKa = 10.34PSGSSRR85 pKa = 11.84SHH87 pKa = 6.85KK88 pKa = 10.4ISS90 pKa = 3.02

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1145

87

533

190.8

21.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.48 ± 1.96

1.048 ± 0.455

5.59 ± 0.762

4.017 ± 0.382

5.939 ± 0.492

8.384 ± 1.438

1.747 ± 0.567

3.93 ± 0.845

4.454 ± 1.457

7.773 ± 0.797

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.231 ± 0.701

4.367 ± 0.681

5.153 ± 1.015

5.066 ± 1.016

6.114 ± 1.259

7.511 ± 1.187

5.939 ± 1.461

4.978 ± 0.71

1.223 ± 0.206

3.057 ± 0.795
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski