Amino acid dipepetide frequency for Neodiprion lecontei nucleopolyhedrovirus (strain Canada) (NeleNPV)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.907AlaAla: 1.907 ± 0.329
1.202AlaCys: 1.202 ± 0.196
2.156AlaAsp: 2.156 ± 0.311
2.031AlaGlu: 2.031 ± 0.335
1.617AlaPhe: 1.617 ± 0.215
1.368AlaGly: 1.368 ± 0.315
0.953AlaHis: 0.953 ± 0.211
2.777AlaIle: 2.777 ± 0.35
3.109AlaLys: 3.109 ± 0.352
2.86AlaLeu: 2.86 ± 0.407
0.705AlaMet: 0.705 ± 0.182
3.233AlaAsn: 3.233 ± 0.317
0.539AlaPro: 0.539 ± 0.181
0.622AlaGln: 0.622 ± 0.125
0.995AlaArg: 0.995 ± 0.177
2.404AlaSer: 2.404 ± 0.329
2.653AlaThr: 2.653 ± 0.361
1.865AlaVal: 1.865 ± 0.257
0.0AlaTrp: 0.0 ± 0.0
1.617AlaTyr: 1.617 ± 0.238
0.041AlaXaa: 0.041 ± 0.041
Cys
0.788CysAla: 0.788 ± 0.183
0.373CysCys: 0.373 ± 0.131
1.99CysAsp: 1.99 ± 0.301
1.036CysGlu: 1.036 ± 0.22
1.409CysPhe: 1.409 ± 0.25
1.451CysGly: 1.451 ± 0.392
0.663CysHis: 0.663 ± 0.154
1.948CysIle: 1.948 ± 0.279
1.368CysLys: 1.368 ± 0.285
2.239CysLeu: 2.239 ± 0.306
0.58CysMet: 0.58 ± 0.164
2.197CysAsn: 2.197 ± 0.308
1.285CysPro: 1.285 ± 0.254
0.995CysGln: 0.995 ± 0.194
1.244CysArg: 1.244 ± 0.23
1.534CysSer: 1.534 ± 0.261
1.741CysThr: 1.741 ± 0.259
2.073CysVal: 2.073 ± 0.308
0.332CysTrp: 0.332 ± 0.129
1.078CysTyr: 1.078 ± 0.183
0.041CysXaa: 0.041 ± 0.043
Asp
2.653AspAla: 2.653 ± 0.31
1.451AspCys: 1.451 ± 0.253
5.721AspAsp: 5.721 ± 0.589
4.145AspGlu: 4.145 ± 0.458
2.57AspPhe: 2.57 ± 0.327
2.695AspGly: 2.695 ± 0.389
0.995AspHis: 0.995 ± 0.249
6.052AspIle: 6.052 ± 0.603
4.684AspLys: 4.684 ± 0.451
4.767AspLeu: 4.767 ± 0.427
1.658AspMet: 1.658 ± 0.299
5.057AspAsn: 5.057 ± 0.571
1.783AspPro: 1.783 ± 0.314
1.658AspGln: 1.658 ± 0.256
1.948AspArg: 1.948 ± 0.339
4.021AspSer: 4.021 ± 0.435
4.228AspThr: 4.228 ± 0.506
6.052AspVal: 6.052 ± 0.513
0.539AspTrp: 0.539 ± 0.137
3.109AspTyr: 3.109 ± 0.391
0.083AspXaa: 0.083 ± 0.054
Glu
1.617GluAla: 1.617 ± 0.312
1.327GluCys: 1.327 ± 0.245
2.695GluAsp: 2.695 ± 0.407
2.114GluGlu: 2.114 ± 0.362
2.197GluPhe: 2.197 ± 0.274
0.663GluGly: 0.663 ± 0.188
1.492GluHis: 1.492 ± 0.241
6.094GluIle: 6.094 ± 0.489
4.145GluLys: 4.145 ± 0.425
4.021GluLeu: 4.021 ± 0.433
1.078GluMet: 1.078 ± 0.192
5.762GluAsn: 5.762 ± 0.526
1.119GluPro: 1.119 ± 0.165
1.948GluGln: 1.948 ± 0.256
2.446GluArg: 2.446 ± 0.368
4.145GluSer: 4.145 ± 0.445
4.311GluThr: 4.311 ± 0.518
1.658GluVal: 1.658 ± 0.259
0.622GluTrp: 0.622 ± 0.171
2.404GluTyr: 2.404 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
2.363PheAla: 2.363 ± 0.336
1.409PheCys: 1.409 ± 0.219
4.145PheAsp: 4.145 ± 0.438
3.151PheGlu: 3.151 ± 0.372
3.026PhePhe: 3.026 ± 0.349
1.327PheGly: 1.327 ± 0.244
0.871PheHis: 0.871 ± 0.178
4.228PheIle: 4.228 ± 0.425
3.772PheLys: 3.772 ± 0.384
4.933PheLeu: 4.933 ± 0.467
1.036PheMet: 1.036 ± 0.181
2.695PheAsn: 2.695 ± 0.329
0.705PhePro: 0.705 ± 0.191
1.492PheGln: 1.492 ± 0.216
2.031PheArg: 2.031 ± 0.309
2.529PheSer: 2.529 ± 0.312
3.026PheThr: 3.026 ± 0.359
4.809PheVal: 4.809 ± 0.321
0.29PheTrp: 0.29 ± 0.101
2.114PheTyr: 2.114 ± 0.236
0.124PheXaa: 0.124 ± 0.073
Gly
0.788GlyAla: 0.788 ± 0.177
1.036GlyCys: 1.036 ± 0.209
2.114GlyAsp: 2.114 ± 0.269
1.368GlyGlu: 1.368 ± 0.216
1.492GlyPhe: 1.492 ± 0.28
1.036GlyGly: 1.036 ± 0.288
0.415GlyHis: 0.415 ± 0.123
2.695GlyIle: 2.695 ± 0.349
1.658GlyLys: 1.658 ± 0.264
3.109GlyLeu: 3.109 ± 0.388
0.29GlyMet: 0.29 ± 0.116
2.073GlyAsn: 2.073 ± 0.264
0.456GlyPro: 0.456 ± 0.149
1.202GlyGln: 1.202 ± 0.241
1.451GlyArg: 1.451 ± 0.265
2.114GlySer: 2.114 ± 0.286
1.658GlyThr: 1.658 ± 0.256
2.363GlyVal: 2.363 ± 0.343
0.373GlyTrp: 0.373 ± 0.147
1.409GlyTyr: 1.409 ± 0.263
0.124GlyXaa: 0.124 ± 0.064
His
0.912HisAla: 0.912 ± 0.16
0.456HisCys: 0.456 ± 0.134
1.7HisAsp: 1.7 ± 0.229
1.202HisGlu: 1.202 ± 0.28
1.285HisPhe: 1.285 ± 0.257
0.829HisGly: 0.829 ± 0.171
0.497HisHis: 0.497 ± 0.146
2.031HisIle: 2.031 ± 0.3
1.534HisLys: 1.534 ± 0.234
2.404HisLeu: 2.404 ± 0.361
0.539HisMet: 0.539 ± 0.127
1.451HisAsn: 1.451 ± 0.251
0.705HisPro: 0.705 ± 0.17
0.705HisGln: 0.705 ± 0.154
1.036HisArg: 1.036 ± 0.21
1.368HisSer: 1.368 ± 0.24
1.327HisThr: 1.327 ± 0.236
2.695HisVal: 2.695 ± 0.396
0.29HisTrp: 0.29 ± 0.106
1.119HisTyr: 1.119 ± 0.224
0.041HisXaa: 0.041 ± 0.039
Ile
3.233IleAla: 3.233 ± 0.385
2.943IleCys: 2.943 ± 0.384
6.342IleAsp: 6.342 ± 0.519
4.726IleGlu: 4.726 ± 0.429
4.643IlePhe: 4.643 ± 0.327
2.487IleGly: 2.487 ± 0.304
2.404IleHis: 2.404 ± 0.408
7.337IleIle: 7.337 ± 0.639
6.218IleLys: 6.218 ± 0.569
7.793IleLeu: 7.793 ± 0.487
2.114IleMet: 2.114 ± 0.297
7.296IleAsn: 7.296 ± 0.496
2.487IlePro: 2.487 ± 0.378
2.902IleGln: 2.902 ± 0.31
3.441IleArg: 3.441 ± 0.385
6.26IleSer: 6.26 ± 0.703
5.845IleThr: 5.845 ± 0.422
6.508IleVal: 6.508 ± 0.45
0.29IleTrp: 0.29 ± 0.141
4.353IleTyr: 4.353 ± 0.456
0.207IleXaa: 0.207 ± 0.088
Lys
1.741LysAla: 1.741 ± 0.287
2.031LysCys: 2.031 ± 0.316
2.777LysAsp: 2.777 ± 0.335
3.026LysGlu: 3.026 ± 0.329
3.938LysPhe: 3.938 ± 0.452
1.327LysGly: 1.327 ± 0.249
3.192LysHis: 3.192 ± 0.406
8.332LysIle: 8.332 ± 0.735
5.721LysLys: 5.721 ± 0.554
6.384LysLeu: 6.384 ± 0.576
1.99LysMet: 1.99 ± 0.342
6.674LysAsn: 6.674 ± 0.591
1.575LysPro: 1.575 ± 0.268
2.28LysGln: 2.28 ± 0.385
3.524LysArg: 3.524 ± 0.382
4.85LysSer: 4.85 ± 0.439
5.762LysThr: 5.762 ± 0.515
1.824LysVal: 1.824 ± 0.265
0.622LysTrp: 0.622 ± 0.15
4.104LysTyr: 4.104 ± 0.367
0.166LysXaa: 0.166 ± 0.087
Leu
3.068LeuAla: 3.068 ± 0.462
2.031LeuCys: 2.031 ± 0.313
3.565LeuAsp: 3.565 ± 0.396
4.145LeuGlu: 4.145 ± 0.35
4.643LeuPhe: 4.643 ± 0.377
2.363LeuGly: 2.363 ± 0.317
2.777LeuHis: 2.777 ± 0.365
7.876LeuIle: 7.876 ± 0.669
7.213LeuLys: 7.213 ± 0.568
9.286LeuLeu: 9.286 ± 0.643
2.239LeuMet: 2.239 ± 0.282
6.342LeuAsn: 6.342 ± 0.533
3.482LeuPro: 3.482 ± 0.384
4.767LeuGln: 4.767 ± 0.443
3.151LeuArg: 3.151 ± 0.407
7.006LeuSer: 7.006 ± 0.521
5.306LeuThr: 5.306 ± 0.461
3.731LeuVal: 3.731 ± 0.348
0.746LeuTrp: 0.746 ± 0.147
5.721LeuTyr: 5.721 ± 0.506
0.207LeuXaa: 0.207 ± 0.082
Met
0.58MetAla: 0.58 ± 0.155
0.995MetCys: 0.995 ± 0.212
1.409MetAsp: 1.409 ± 0.276
1.078MetGlu: 1.078 ± 0.215
1.368MetPhe: 1.368 ± 0.246
0.29MetGly: 0.29 ± 0.1
0.539MetHis: 0.539 ± 0.148
1.783MetIle: 1.783 ± 0.271
1.409MetLys: 1.409 ± 0.241
2.529MetLeu: 2.529 ± 0.342
0.539MetMet: 0.539 ± 0.178
1.824MetAsn: 1.824 ± 0.318
0.871MetPro: 0.871 ± 0.178
0.912MetGln: 0.912 ± 0.194
1.161MetArg: 1.161 ± 0.232
1.948MetSer: 1.948 ± 0.279
1.7MetThr: 1.7 ± 0.259
0.912MetVal: 0.912 ± 0.218
0.249MetTrp: 0.249 ± 0.094
0.953MetTyr: 0.953 ± 0.202
0.083MetXaa: 0.083 ± 0.053
Asn
3.068AsnAla: 3.068 ± 0.324
1.575AsnCys: 1.575 ± 0.257
5.223AsnAsp: 5.223 ± 0.499
5.265AsnGlu: 5.265 ± 0.595
3.938AsnPhe: 3.938 ± 0.442
2.653AsnGly: 2.653 ± 0.344
1.285AsnHis: 1.285 ± 0.206
8.374AsnIle: 8.374 ± 0.541
5.845AsnLys: 5.845 ± 0.64
7.047AsnLeu: 7.047 ± 0.599
2.073AsnMet: 2.073 ± 0.269
6.384AsnAsn: 6.384 ± 0.462
1.7AsnPro: 1.7 ± 0.229
2.363AsnGln: 2.363 ± 0.327
3.192AsnArg: 3.192 ± 0.397
4.436AsnSer: 4.436 ± 0.391
5.182AsnThr: 5.182 ± 0.509
7.296AsnVal: 7.296 ± 0.611
0.456AsnTrp: 0.456 ± 0.145
3.316AsnTyr: 3.316 ± 0.36
0.124AsnXaa: 0.124 ± 0.069
Pro
0.912ProAla: 0.912 ± 0.221
0.539ProCys: 0.539 ± 0.193
1.658ProAsp: 1.658 ± 0.208
1.409ProGlu: 1.409 ± 0.245
1.327ProPhe: 1.327 ± 0.27
0.746ProGly: 0.746 ± 0.196
0.746ProHis: 0.746 ± 0.152
2.653ProIle: 2.653 ± 0.299
1.741ProLys: 1.741 ± 0.308
2.28ProLeu: 2.28 ± 0.321
0.497ProMet: 0.497 ± 0.147
2.239ProAsn: 2.239 ± 0.322
0.663ProPro: 0.663 ± 0.166
0.995ProGln: 0.995 ± 0.212
1.119ProArg: 1.119 ± 0.279
1.741ProSer: 1.741 ± 0.243
1.907ProThr: 1.907 ± 0.276
1.824ProVal: 1.824 ± 0.277
0.083ProTrp: 0.083 ± 0.056
1.492ProTyr: 1.492 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
1.036GlnAla: 1.036 ± 0.218
0.953GlnCys: 0.953 ± 0.195
1.409GlnAsp: 1.409 ± 0.207
1.783GlnGlu: 1.783 ± 0.295
1.202GlnPhe: 1.202 ± 0.19
0.539GlnGly: 0.539 ± 0.144
1.036GlnHis: 1.036 ± 0.2
4.394GlnIle: 4.394 ± 0.458
3.109GlnLys: 3.109 ± 0.379
3.648GlnLeu: 3.648 ± 0.36
0.995GlnMet: 0.995 ± 0.214
3.482GlnAsn: 3.482 ± 0.333
1.036GlnPro: 1.036 ± 0.23
1.327GlnGln: 1.327 ± 0.26
1.285GlnArg: 1.285 ± 0.248
1.865GlnSer: 1.865 ± 0.274
3.026GlnThr: 3.026 ± 0.391
0.788GlnVal: 0.788 ± 0.155
0.166GlnTrp: 0.166 ± 0.07
1.409GlnTyr: 1.409 ± 0.204
0.083GlnXaa: 0.083 ± 0.059
Arg
1.078ArgAla: 1.078 ± 0.207
1.451ArgCys: 1.451 ± 0.275
2.363ArgAsp: 2.363 ± 0.338
1.865ArgGlu: 1.865 ± 0.315
2.073ArgPhe: 2.073 ± 0.296
0.912ArgGly: 0.912 ± 0.185
0.912ArgHis: 0.912 ± 0.178
3.151ArgIle: 3.151 ± 0.327
3.109ArgLys: 3.109 ± 0.342
4.436ArgLeu: 4.436 ± 0.494
1.161ArgMet: 1.161 ± 0.202
3.316ArgAsn: 3.316 ± 0.351
1.036ArgPro: 1.036 ± 0.242
1.824ArgGln: 1.824 ± 0.281
2.073ArgArg: 2.073 ± 0.313
3.855ArgSer: 3.855 ± 0.785
2.197ArgThr: 2.197 ± 0.302
1.948ArgVal: 1.948 ± 0.374
0.249ArgTrp: 0.249 ± 0.107
2.28ArgTyr: 2.28 ± 0.328
0.207ArgXaa: 0.207 ± 0.086
Ser
2.197SerAla: 2.197 ± 0.355
1.617SerCys: 1.617 ± 0.225
6.094SerAsp: 6.094 ± 0.498
3.607SerGlu: 3.607 ± 0.372
3.938SerPhe: 3.938 ± 0.409
2.321SerGly: 2.321 ± 0.427
1.285SerHis: 1.285 ± 0.248
5.182SerIle: 5.182 ± 0.443
4.519SerLys: 4.519 ± 0.494
5.845SerLeu: 5.845 ± 0.44
1.327SerMet: 1.327 ± 0.218
5.513SerAsn: 5.513 ± 0.41
1.824SerPro: 1.824 ± 0.274
2.031SerGln: 2.031 ± 0.252
3.441SerArg: 3.441 ± 0.568
5.306SerSer: 5.306 ± 0.519
5.596SerThr: 5.596 ± 0.581
4.477SerVal: 4.477 ± 0.406
0.871SerTrp: 0.871 ± 0.191
2.819SerTyr: 2.819 ± 0.341
0.166SerXaa: 0.166 ± 0.092
Thr
2.612ThrAla: 2.612 ± 0.398
1.327ThrCys: 1.327 ± 0.246
4.767ThrAsp: 4.767 ± 0.41
2.985ThrGlu: 2.985 ± 0.37
3.358ThrPhe: 3.358 ± 0.389
2.031ThrGly: 2.031 ± 0.297
1.285ThrHis: 1.285 ± 0.196
5.845ThrIle: 5.845 ± 0.581
4.021ThrLys: 4.021 ± 0.323
5.928ThrLeu: 5.928 ± 0.479
1.741ThrMet: 1.741 ± 0.262
5.804ThrAsn: 5.804 ± 0.58
2.28ThrPro: 2.28 ± 0.299
2.404ThrGln: 2.404 ± 0.319
3.026ThrArg: 3.026 ± 0.347
6.052ThrSer: 6.052 ± 0.552
5.721ThrThr: 5.721 ± 0.548
3.731ThrVal: 3.731 ± 0.451
0.539ThrTrp: 0.539 ± 0.14
2.819ThrTyr: 2.819 ± 0.36
0.207ThrXaa: 0.207 ± 0.097
Val
1.783ValAla: 1.783 ± 0.277
1.658ValCys: 1.658 ± 0.319
5.596ValAsp: 5.596 ± 0.526
4.063ValGlu: 4.063 ± 0.421
3.026ValPhe: 3.026 ± 0.327
1.7ValGly: 1.7 ± 0.276
1.368ValHis: 1.368 ± 0.26
4.311ValIle: 4.311 ± 0.491
4.27ValLys: 4.27 ± 0.434
4.933ValLeu: 4.933 ± 0.51
1.409ValMet: 1.409 ± 0.218
4.892ValAsn: 4.892 ± 0.453
1.783ValPro: 1.783 ± 0.307
2.487ValGln: 2.487 ± 0.323
2.57ValArg: 2.57 ± 0.257
4.85ValSer: 4.85 ± 0.465
3.607ValThr: 3.607 ± 0.409
4.85ValVal: 4.85 ± 0.448
0.58ValTrp: 0.58 ± 0.166
3.565ValTyr: 3.565 ± 0.416
0.041ValXaa: 0.041 ± 0.051
Trp
0.249TrpAla: 0.249 ± 0.085
0.249TrpCys: 0.249 ± 0.09
0.249TrpAsp: 0.249 ± 0.091
0.249TrpGlu: 0.249 ± 0.095
0.497TrpPhe: 0.497 ± 0.151
0.456TrpGly: 0.456 ± 0.159
0.249TrpHis: 0.249 ± 0.087
0.332TrpIle: 0.332 ± 0.122
0.58TrpLys: 0.58 ± 0.176
0.829TrpLeu: 0.829 ± 0.197
0.166TrpMet: 0.166 ± 0.088
0.58TrpAsn: 0.58 ± 0.145
0.29TrpPro: 0.29 ± 0.095
0.497TrpGln: 0.497 ± 0.139
0.29TrpArg: 0.29 ± 0.099
0.788TrpSer: 0.788 ± 0.16
0.539TrpThr: 0.539 ± 0.154
0.166TrpVal: 0.166 ± 0.072
0.124TrpTrp: 0.124 ± 0.064
0.249TrpTyr: 0.249 ± 0.108
0.041TrpXaa: 0.041 ± 0.045
Tyr
1.907TyrAla: 1.907 ± 0.229
1.575TyrCys: 1.575 ± 0.253
3.731TyrAsp: 3.731 ± 0.414
2.529TyrGlu: 2.529 ± 0.402
2.28TyrPhe: 2.28 ± 0.361
1.907TyrGly: 1.907 ± 0.279
1.161TyrHis: 1.161 ± 0.194
4.27TyrIle: 4.27 ± 0.4
3.731TyrLys: 3.731 ± 0.372
4.228TyrLeu: 4.228 ± 0.323
0.995TyrMet: 0.995 ± 0.211
3.772TyrAsn: 3.772 ± 0.395
0.912TyrPro: 0.912 ± 0.185
1.161TyrGln: 1.161 ± 0.238
1.99TyrArg: 1.99 ± 0.264
2.736TyrSer: 2.736 ± 0.307
3.068TyrThr: 3.068 ± 0.369
3.648TyrVal: 3.648 ± 0.464
0.249TyrTrp: 0.249 ± 0.084
2.28TyrTyr: 2.28 ± 0.291
0.124TyrXaa: 0.124 ± 0.079
Xaa
0.166XaaAla: 0.166 ± 0.077
0.166XaaCys: 0.166 ± 0.072
0.166XaaAsp: 0.166 ± 0.078
0.083XaaGlu: 0.083 ± 0.058
0.249XaaPhe: 0.249 ± 0.115
0.124XaaGly: 0.124 ± 0.07
0.0XaaHis: 0.0 ± 0.0
0.166XaaIle: 0.166 ± 0.08
0.083XaaLys: 0.083 ± 0.061
0.166XaaLeu: 0.166 ± 0.077
0.0XaaMet: 0.0 ± 0.0
0.207XaaAsn: 0.207 ± 0.101
0.041XaaPro: 0.041 ± 0.036
0.0XaaGln: 0.0 ± 0.0
0.083XaaArg: 0.083 ± 0.055
0.166XaaSer: 0.166 ± 0.075
0.083XaaThr: 0.083 ± 0.054
0.124XaaVal: 0.124 ± 0.064
0.0XaaTrp: 0.0 ± 0.0
0.041XaaTyr: 0.041 ± 0.041
0.083XaaXaa: 0.083 ± 0.058
Statistics based on 89 proteins (24124 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski