Arthrobacter sp. U41
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4060 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C9WQX5|A0A1C9WQX5_9MICC Tyr recombinase domain-containing protein OS=Arthrobacter sp. U41 OX=1849032 GN=ASPU41_08770 PE=4 SV=1
MM1 pKa = 7.38 ARR3 pKa = 11.84 VAPEE7 pKa = 3.78 LQYY10 pKa = 11.19 SDD12 pKa = 3.22 EE13 pKa = 4.79 HH14 pKa = 6.26 EE15 pKa = 4.56 WIARR19 pKa = 11.84 EE20 pKa = 3.91 AGNVVSIGISAVATDD35 pKa = 3.79 ALGDD39 pKa = 3.62 IVYY42 pKa = 10.59 VDD44 pKa = 4.3 LPEE47 pKa = 4.75 VGATVTAGEE56 pKa = 4.35 TCGEE60 pKa = 4.21 VEE62 pKa = 4.36 STKK65 pKa = 10.7 SVSDD69 pKa = 4.05 LYY71 pKa = 11.58 SPVTGEE77 pKa = 3.86 VTEE80 pKa = 4.26 INPAVVDD87 pKa = 5.0 DD88 pKa = 4.26 PALINSDD95 pKa = 3.62 PYY97 pKa = 10.15 GAGWLFKK104 pKa = 10.68 VAAEE108 pKa = 4.13 SDD110 pKa = 4.13 GPLLSAEE117 pKa = 4.57 EE118 pKa = 4.16 YY119 pKa = 10.15 ASKK122 pKa = 11.09 NGGEE126 pKa = 4.13 LL127 pKa = 3.41
Molecular weight: 13.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.541
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.592
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.63
Nozaki 3.821
DTASelect 3.961
Thurlkill 3.579
EMBOSS 3.617
Sillero 3.834
Patrickios 1.825
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|A0A1C9WWH7|A0A1C9WWH7_9MICC CsbD-like protein OS=Arthrobacter sp. U41 OX=1849032 GN=ASPU41_14250 PE=3 SV=1
MM1 pKa = 7.27 VIVAVAFIAALFIGALISIARR22 pKa = 11.84 SRR24 pKa = 11.84 NHH26 pKa = 5.88 TPAGAALWVLAVAALPVLGPVLWFLTGRR54 pKa = 11.84 TPSRR58 pKa = 11.84 PGRR61 pKa = 11.84 PGSPP65 pKa = 3.12
Molecular weight: 6.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4060
0
4060
1290115
37
2603
317.8
34.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.184 ± 0.051
0.634 ± 0.011
5.546 ± 0.034
5.721 ± 0.036
3.201 ± 0.023
9.13 ± 0.037
2.104 ± 0.019
4.345 ± 0.024
2.829 ± 0.027
10.272 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.004 ± 0.015
2.385 ± 0.021
5.512 ± 0.032
3.108 ± 0.02
6.682 ± 0.039
5.838 ± 0.028
5.835 ± 0.03
8.181 ± 0.03
1.415 ± 0.016
2.074 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here