Diplodia seriata

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae; Diplodia

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8038 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S8B9C2|A0A1S8B9C2_9PEZI ATP-grasp domain-containing protein OS=Diplodia seriata OX=420778 GN=BK809_0001532 PE=4 SV=1
MM1 pKa = 6.88VQINNALAGMIVGAGALVAAAPAPAPTAAPNLKK34 pKa = 9.89DD35 pKa = 3.43AQIGKK40 pKa = 9.59RR41 pKa = 11.84ADD43 pKa = 2.99ACTFTDD49 pKa = 4.24ASAASASKK57 pKa = 10.46ASCSTIILSDD67 pKa = 3.02ITVPAGEE74 pKa = 4.4TLDD77 pKa = 3.72MTDD80 pKa = 5.37LEE82 pKa = 5.02DD83 pKa = 3.37NTYY86 pKa = 10.71VEE88 pKa = 4.74FQGTTTFEE96 pKa = 3.9YY97 pKa = 10.94DD98 pKa = 2.94EE99 pKa = 4.51WEE101 pKa = 4.63GPLVSFSGTGITITGASGHH120 pKa = 5.71VLDD123 pKa = 5.83GNGAKK128 pKa = 8.87WWDD131 pKa = 3.73GEE133 pKa = 4.25GSNGGVTKK141 pKa = 10.54PKK143 pKa = 10.33FFYY146 pKa = 10.52AHH148 pKa = 6.06SLKK151 pKa = 10.9SSTIKK156 pKa = 10.71GLNIKK161 pKa = 7.66NTPVQCMSINGAEE174 pKa = 4.11EE175 pKa = 6.03LYY177 pKa = 11.04VQDD180 pKa = 3.45ITIDD184 pKa = 3.71NTDD187 pKa = 3.35GDD189 pKa = 4.27ATNDD193 pKa = 3.55DD194 pKa = 4.3GDD196 pKa = 4.35ALGHH200 pKa = 5.75NTDD203 pKa = 3.95AFDD206 pKa = 3.84IGSSTGVYY214 pKa = 9.77ISGANVQNQDD224 pKa = 2.67DD225 pKa = 4.17CMAINSGEE233 pKa = 4.49NISMMDD239 pKa = 3.81GTCSGGHH246 pKa = 5.92GLSIGSVGGRR256 pKa = 11.84SDD258 pKa = 4.44NDD260 pKa = 3.39VKK262 pKa = 11.25NVTVSGMTVKK272 pKa = 10.67NSQNGVRR279 pKa = 11.84IKK281 pKa = 9.73TVYY284 pKa = 10.35DD285 pKa = 3.33ATGSVSDD292 pKa = 3.93ITYY295 pKa = 10.91SDD297 pKa = 3.18ITLSGITKK305 pKa = 8.93YY306 pKa = 10.97GIVIEE311 pKa = 3.95QDD313 pKa = 3.68YY314 pKa = 11.2EE315 pKa = 4.22NGSPTGEE322 pKa = 4.18PTDD325 pKa = 3.87GVPITGLTIDD335 pKa = 4.09GVTGSVEE342 pKa = 4.5DD343 pKa = 4.62DD344 pKa = 3.19ATPIYY349 pKa = 10.05ILCADD354 pKa = 4.99GACSDD359 pKa = 4.07WSWSADD365 pKa = 3.1ITGGEE370 pKa = 4.19ASTEE374 pKa = 4.0CSGIPDD380 pKa = 4.02GASCC384 pKa = 5.2

Molecular weight: 39.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S8BP68|A0A1S8BP68_9PEZI MBOAT_2 domain-containing protein OS=Diplodia seriata OX=420778 GN=BK809_0005957 PE=4 SV=1
SS1 pKa = 6.27QKK3 pKa = 10.7SFRR6 pKa = 11.84TKK8 pKa = 10.38QKK10 pKa = 9.84LAKK13 pKa = 9.55AQKK16 pKa = 8.59QNRR19 pKa = 11.84PIPQWIRR26 pKa = 11.84LRR28 pKa = 11.84TGNTIRR34 pKa = 11.84YY35 pKa = 5.79NAKK38 pKa = 8.89RR39 pKa = 11.84RR40 pKa = 11.84HH41 pKa = 4.15WRR43 pKa = 11.84KK44 pKa = 7.38TRR46 pKa = 11.84IGII49 pKa = 4.0

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8038

0

8038

4002348

49

5079

497.9

54.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.719 ± 0.029

1.141 ± 0.009

5.828 ± 0.018

6.244 ± 0.028

3.681 ± 0.016

7.414 ± 0.029

2.349 ± 0.011

4.351 ± 0.017

4.74 ± 0.026

8.666 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.129 ± 0.01

3.474 ± 0.013

6.101 ± 0.027

3.863 ± 0.02

6.24 ± 0.023

7.744 ± 0.034

5.759 ± 0.017

6.357 ± 0.021

1.489 ± 0.011

2.712 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski