Escherichia phage JSS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9SEA5|A0A1I9SEA5_9CAUD HNH homing endonuclease OS=Escherichia phage JSS1 OX=1897443 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.17YY9 pKa = 10.19EE10 pKa = 4.4LLAATVEE17 pKa = 4.25AFNEE21 pKa = 4.27RR22 pKa = 11.84IQYY25 pKa = 10.74DD26 pKa = 4.22KK27 pKa = 10.88IAEE30 pKa = 4.25GDD32 pKa = 3.85DD33 pKa = 3.77YY34 pKa = 11.79SDD36 pKa = 4.02ALHH39 pKa = 6.33EE40 pKa = 4.42VVDD43 pKa = 4.7GNVPHH48 pKa = 6.9YY49 pKa = 8.99YY50 pKa = 10.31HH51 pKa = 7.6EE52 pKa = 4.77IFTVMAADD60 pKa = 5.4GIDD63 pKa = 3.84HH64 pKa = 6.56EE65 pKa = 5.23FEE67 pKa = 5.3DD68 pKa = 4.36SGLMPEE74 pKa = 4.69TKK76 pKa = 10.16DD77 pKa = 3.27VTHH80 pKa = 7.19ILQARR85 pKa = 11.84IYY87 pKa = 8.31EE88 pKa = 4.05ALYY91 pKa = 10.11IDD93 pKa = 4.29VSNSSDD99 pKa = 3.83VVWFEE104 pKa = 4.48AEE106 pKa = 4.15EE107 pKa = 4.4GDD109 pKa = 4.25EE110 pKa = 4.07

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9SEA2|A0A1I9SEA2_9CAUD Uncharacterized protein OS=Escherichia phage JSS1 OX=1897443 PE=4 SV=1
MM1 pKa = 7.64ISALLIAIGYY11 pKa = 8.61GLIAYY16 pKa = 8.18VLVRR20 pKa = 11.84DD21 pKa = 3.6INKK24 pKa = 9.28ARR26 pKa = 11.84KK27 pKa = 8.78VYY29 pKa = 9.84KK30 pKa = 10.31LNYY33 pKa = 8.2VRR35 pKa = 11.84LVRR38 pKa = 11.84WTVRR42 pKa = 11.84QPNGRR47 pKa = 11.84FMRR50 pKa = 11.84NLANVWDD57 pKa = 4.02IATLGSKK64 pKa = 10.07LL65 pKa = 3.58

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

11600

49

1295

257.8

28.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.707 ± 0.445

0.836 ± 0.151

6.543 ± 0.263

6.664 ± 0.333

3.647 ± 0.188

7.94 ± 0.33

1.759 ± 0.215

5.043 ± 0.165

6.483 ± 0.356

7.905 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.161

4.543 ± 0.254

3.75 ± 0.208

3.784 ± 0.379

5.5 ± 0.198

6.112 ± 0.347

5.871 ± 0.25

7.112 ± 0.297

1.483 ± 0.175

3.543 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski