Amino acid dipepetide frequency for Escherichia phage JSS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.708AlaAla: 8.708 ± 1.073
0.776AlaCys: 0.776 ± 0.256
5.604AlaAsp: 5.604 ± 0.775
5.949AlaGlu: 5.949 ± 0.909
3.707AlaPhe: 3.707 ± 0.592
6.983AlaGly: 6.983 ± 1.127
0.862AlaHis: 0.862 ± 0.288
5.518AlaIle: 5.518 ± 0.769
5.776AlaLys: 5.776 ± 0.666
7.07AlaLeu: 7.07 ± 1.221
2.673AlaMet: 2.673 ± 0.418
3.276AlaAsn: 3.276 ± 0.396
2.845AlaPro: 2.845 ± 0.529
2.931AlaGln: 2.931 ± 0.588
4.138AlaArg: 4.138 ± 0.582
4.569AlaSer: 4.569 ± 0.454
4.311AlaThr: 4.311 ± 0.52
6.983AlaVal: 6.983 ± 1.019
1.724AlaTrp: 1.724 ± 0.441
2.673AlaTyr: 2.673 ± 0.538
0.0AlaXaa: 0.0 ± 0.0
Cys
0.431CysAla: 0.431 ± 0.156
0.172CysCys: 0.172 ± 0.171
0.69CysAsp: 0.69 ± 0.336
0.604CysGlu: 0.604 ± 0.163
0.604CysPhe: 0.604 ± 0.231
0.259CysGly: 0.259 ± 0.163
0.172CysHis: 0.172 ± 0.115
0.345CysIle: 0.345 ± 0.17
0.604CysLys: 0.604 ± 0.271
0.776CysLeu: 0.776 ± 0.326
0.259CysMet: 0.259 ± 0.195
0.259CysAsn: 0.259 ± 0.141
0.431CysPro: 0.431 ± 0.184
0.259CysGln: 0.259 ± 0.161
0.517CysArg: 0.517 ± 0.247
0.604CysSer: 0.604 ± 0.248
0.172CysThr: 0.172 ± 0.115
0.776CysVal: 0.776 ± 0.258
0.172CysTrp: 0.172 ± 0.117
0.259CysTyr: 0.259 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
6.466AspAla: 6.466 ± 0.638
0.517AspCys: 0.517 ± 0.268
4.483AspAsp: 4.483 ± 0.67
3.535AspGlu: 3.535 ± 0.501
2.155AspPhe: 2.155 ± 0.509
6.897AspGly: 6.897 ± 0.666
1.379AspHis: 1.379 ± 0.332
3.362AspIle: 3.362 ± 0.504
3.449AspLys: 3.449 ± 0.586
5.087AspLeu: 5.087 ± 0.585
2.328AspMet: 2.328 ± 0.375
2.328AspAsn: 2.328 ± 0.491
3.018AspPro: 3.018 ± 0.73
1.638AspGln: 1.638 ± 0.352
2.5AspArg: 2.5 ± 0.519
3.793AspSer: 3.793 ± 0.491
3.793AspThr: 3.793 ± 0.695
5.173AspVal: 5.173 ± 0.735
1.207AspTrp: 1.207 ± 0.35
2.328AspTyr: 2.328 ± 0.417
0.0AspXaa: 0.0 ± 0.0
Glu
6.466GluAla: 6.466 ± 0.974
0.345GluCys: 0.345 ± 0.169
4.742GluAsp: 4.742 ± 0.689
4.656GluGlu: 4.656 ± 0.719
2.759GluPhe: 2.759 ± 0.533
5.173GluGly: 5.173 ± 0.86
0.948GluHis: 0.948 ± 0.281
2.586GluIle: 2.586 ± 0.401
3.276GluLys: 3.276 ± 0.444
6.121GluLeu: 6.121 ± 0.792
1.897GluMet: 1.897 ± 0.386
2.586GluAsn: 2.586 ± 0.52
2.155GluPro: 2.155 ± 0.473
2.759GluGln: 2.759 ± 0.511
3.88GluArg: 3.88 ± 0.613
3.707GluSer: 3.707 ± 0.686
3.966GluThr: 3.966 ± 0.378
3.793GluVal: 3.793 ± 0.647
1.466GluTrp: 1.466 ± 0.327
3.276GluTyr: 3.276 ± 0.502
0.0GluXaa: 0.0 ± 0.0
Phe
3.362PheAla: 3.362 ± 0.455
0.259PheCys: 0.259 ± 0.187
3.19PheAsp: 3.19 ± 0.599
1.811PheGlu: 1.811 ± 0.34
1.379PhePhe: 1.379 ± 0.389
2.328PheGly: 2.328 ± 0.515
0.69PheHis: 0.69 ± 0.221
1.552PheIle: 1.552 ± 0.437
2.931PheLys: 2.931 ± 0.445
2.759PheLeu: 2.759 ± 0.475
0.69PheMet: 0.69 ± 0.247
2.759PheAsn: 2.759 ± 0.489
1.293PhePro: 1.293 ± 0.308
0.776PheGln: 0.776 ± 0.238
1.466PheArg: 1.466 ± 0.294
2.328PheSer: 2.328 ± 0.404
2.586PheThr: 2.586 ± 0.266
2.673PheVal: 2.673 ± 0.58
0.431PheTrp: 0.431 ± 0.146
1.207PheTyr: 1.207 ± 0.273
0.0PheXaa: 0.0 ± 0.0
Gly
6.294GlyAla: 6.294 ± 0.908
0.345GlyCys: 0.345 ± 0.192
5.259GlyAsp: 5.259 ± 0.9
5.604GlyGlu: 5.604 ± 0.546
2.155GlyPhe: 2.155 ± 0.437
5.863GlyGly: 5.863 ± 0.716
1.293GlyHis: 1.293 ± 0.343
4.569GlyIle: 4.569 ± 0.704
5.863GlyLys: 5.863 ± 1.004
5.776GlyLeu: 5.776 ± 0.757
2.673GlyMet: 2.673 ± 0.525
2.673GlyAsn: 2.673 ± 0.408
1.207GlyPro: 1.207 ± 0.324
2.931GlyGln: 2.931 ± 0.417
5.69GlyArg: 5.69 ± 0.533
5.863GlySer: 5.863 ± 0.693
4.483GlyThr: 4.483 ± 0.488
5.69GlyVal: 5.69 ± 0.605
1.207GlyTrp: 1.207 ± 0.296
3.966GlyTyr: 3.966 ± 0.639
0.0GlyXaa: 0.0 ± 0.0
His
0.604HisAla: 0.604 ± 0.247
0.259HisCys: 0.259 ± 0.143
1.379HisAsp: 1.379 ± 0.496
1.293HisGlu: 1.293 ± 0.439
0.345HisPhe: 0.345 ± 0.23
1.035HisGly: 1.035 ± 0.316
0.345HisHis: 0.345 ± 0.172
1.379HisIle: 1.379 ± 0.259
1.293HisLys: 1.293 ± 0.39
1.811HisLeu: 1.811 ± 0.458
0.431HisMet: 0.431 ± 0.216
0.517HisAsn: 0.517 ± 0.184
0.517HisPro: 0.517 ± 0.212
0.604HisGln: 0.604 ± 0.208
1.121HisArg: 1.121 ± 0.281
1.293HisSer: 1.293 ± 0.288
1.207HisThr: 1.207 ± 0.317
1.207HisVal: 1.207 ± 0.277
0.345HisTrp: 0.345 ± 0.186
0.604HisTyr: 0.604 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
4.828IleAla: 4.828 ± 0.693
0.776IleCys: 0.776 ± 0.273
3.19IleAsp: 3.19 ± 0.362
3.362IleGlu: 3.362 ± 0.424
1.035IlePhe: 1.035 ± 0.302
4.138IleGly: 4.138 ± 0.526
1.121IleHis: 1.121 ± 0.376
2.155IleIle: 2.155 ± 0.382
4.052IleLys: 4.052 ± 0.581
2.931IleLeu: 2.931 ± 0.47
0.862IleMet: 0.862 ± 0.213
2.845IleAsn: 2.845 ± 0.512
1.983IlePro: 1.983 ± 0.493
1.552IleGln: 1.552 ± 0.485
2.931IleArg: 2.931 ± 0.559
2.845IleSer: 2.845 ± 0.513
3.018IleThr: 3.018 ± 0.479
4.656IleVal: 4.656 ± 0.394
0.69IleTrp: 0.69 ± 0.255
1.466IleTyr: 1.466 ± 0.316
0.0IleXaa: 0.0 ± 0.0
Lys
7.07LysAla: 7.07 ± 0.642
0.776LysCys: 0.776 ± 0.294
4.052LysAsp: 4.052 ± 0.456
3.535LysGlu: 3.535 ± 0.432
2.414LysPhe: 2.414 ± 0.559
4.138LysGly: 4.138 ± 0.627
1.207LysHis: 1.207 ± 0.458
2.242LysIle: 2.242 ± 0.296
3.449LysLys: 3.449 ± 0.857
5.259LysLeu: 5.259 ± 0.6
1.897LysMet: 1.897 ± 0.361
2.414LysAsn: 2.414 ± 0.457
3.018LysPro: 3.018 ± 0.708
2.328LysGln: 2.328 ± 0.532
4.311LysArg: 4.311 ± 0.71
4.052LysSer: 4.052 ± 0.515
4.052LysThr: 4.052 ± 0.486
4.914LysVal: 4.914 ± 0.851
1.121LysTrp: 1.121 ± 0.355
2.586LysTyr: 2.586 ± 0.445
0.0LysXaa: 0.0 ± 0.0
Leu
6.035LeuAla: 6.035 ± 0.716
0.345LeuCys: 0.345 ± 0.178
4.311LeuAsp: 4.311 ± 0.479
6.639LeuGlu: 6.639 ± 0.884
2.242LeuPhe: 2.242 ± 0.406
4.914LeuGly: 4.914 ± 0.848
0.862LeuHis: 0.862 ± 0.253
3.966LeuIle: 3.966 ± 0.622
6.983LeuLys: 6.983 ± 0.695
4.742LeuLeu: 4.742 ± 0.663
2.931LeuMet: 2.931 ± 0.466
4.225LeuAsn: 4.225 ± 0.609
3.362LeuPro: 3.362 ± 0.383
3.707LeuGln: 3.707 ± 0.64
5.604LeuArg: 5.604 ± 0.542
5.087LeuSer: 5.087 ± 0.643
5.087LeuThr: 5.087 ± 0.667
4.742LeuVal: 4.742 ± 0.604
0.862LeuTrp: 0.862 ± 0.293
2.414LeuTyr: 2.414 ± 0.52
0.0LeuXaa: 0.0 ± 0.0
Met
3.018MetAla: 3.018 ± 0.49
0.431MetCys: 0.431 ± 0.201
1.466MetAsp: 1.466 ± 0.356
2.069MetGlu: 2.069 ± 0.404
1.293MetPhe: 1.293 ± 0.296
2.242MetGly: 2.242 ± 0.43
0.259MetHis: 0.259 ± 0.14
1.293MetIle: 1.293 ± 0.257
1.207MetLys: 1.207 ± 0.299
2.5MetLeu: 2.5 ± 0.403
0.604MetMet: 0.604 ± 0.213
1.121MetAsn: 1.121 ± 0.259
0.948MetPro: 0.948 ± 0.359
0.948MetGln: 0.948 ± 0.317
1.121MetArg: 1.121 ± 0.313
1.638MetSer: 1.638 ± 0.359
2.069MetThr: 2.069 ± 0.377
2.5MetVal: 2.5 ± 0.387
0.259MetTrp: 0.259 ± 0.135
0.776MetTyr: 0.776 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
4.483AsnAla: 4.483 ± 0.595
0.431AsnCys: 0.431 ± 0.223
1.983AsnAsp: 1.983 ± 0.427
2.414AsnGlu: 2.414 ± 0.44
1.379AsnPhe: 1.379 ± 0.245
5.087AsnGly: 5.087 ± 0.717
0.948AsnHis: 0.948 ± 0.301
2.328AsnIle: 2.328 ± 0.408
2.673AsnLys: 2.673 ± 0.467
3.793AsnLeu: 3.793 ± 0.705
1.379AsnMet: 1.379 ± 0.334
2.414AsnAsn: 2.414 ± 0.627
2.931AsnPro: 2.931 ± 0.421
1.466AsnGln: 1.466 ± 0.368
2.242AsnArg: 2.242 ± 0.53
2.328AsnSer: 2.328 ± 0.505
2.673AsnThr: 2.673 ± 0.871
2.759AsnVal: 2.759 ± 0.455
0.259AsnTrp: 0.259 ± 0.15
1.466AsnTyr: 1.466 ± 0.419
0.0AsnXaa: 0.0 ± 0.0
Pro
2.759ProAla: 2.759 ± 0.579
0.604ProCys: 0.604 ± 0.271
2.673ProAsp: 2.673 ± 0.382
3.19ProGlu: 3.19 ± 0.566
1.293ProPhe: 1.293 ± 0.243
1.379ProGly: 1.379 ± 0.362
0.517ProHis: 0.517 ± 0.133
1.983ProIle: 1.983 ± 0.391
3.018ProLys: 3.018 ± 0.552
1.724ProLeu: 1.724 ± 0.395
0.948ProMet: 0.948 ± 0.308
2.414ProAsn: 2.414 ± 0.45
0.776ProPro: 0.776 ± 0.217
1.811ProGln: 1.811 ± 0.299
1.552ProArg: 1.552 ± 0.312
2.845ProSer: 2.845 ± 0.415
2.931ProThr: 2.931 ± 0.387
3.362ProVal: 3.362 ± 0.456
0.69ProTrp: 0.69 ± 0.29
1.035ProTyr: 1.035 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
4.138GlnAla: 4.138 ± 0.499
0.0GlnCys: 0.0 ± 0.0
3.018GlnAsp: 3.018 ± 0.912
2.414GlnGlu: 2.414 ± 0.417
1.724GlnPhe: 1.724 ± 0.31
2.586GlnGly: 2.586 ± 0.521
0.604GlnHis: 0.604 ± 0.219
1.379GlnIle: 1.379 ± 0.315
2.328GlnLys: 2.328 ± 0.465
4.225GlnLeu: 4.225 ± 0.662
1.293GlnMet: 1.293 ± 0.364
1.293GlnAsn: 1.293 ± 0.422
0.776GlnPro: 0.776 ± 0.276
1.724GlnGln: 1.724 ± 0.498
2.155GlnArg: 2.155 ± 0.558
2.673GlnSer: 2.673 ± 0.454
2.069GlnThr: 2.069 ± 0.483
1.983GlnVal: 1.983 ± 0.367
0.431GlnTrp: 0.431 ± 0.164
1.035GlnTyr: 1.035 ± 0.331
0.0GlnXaa: 0.0 ± 0.0
Arg
4.569ArgAla: 4.569 ± 0.798
0.431ArgCys: 0.431 ± 0.186
4.397ArgAsp: 4.397 ± 0.455
3.793ArgGlu: 3.793 ± 0.503
2.414ArgPhe: 2.414 ± 0.348
4.225ArgGly: 4.225 ± 0.417
0.862ArgHis: 0.862 ± 0.285
3.88ArgIle: 3.88 ± 0.712
3.018ArgLys: 3.018 ± 0.637
5.949ArgLeu: 5.949 ± 0.643
0.776ArgMet: 0.776 ± 0.276
2.759ArgAsn: 2.759 ± 0.527
1.811ArgPro: 1.811 ± 0.353
2.673ArgGln: 2.673 ± 0.44
2.414ArgArg: 2.414 ± 0.374
3.535ArgSer: 3.535 ± 0.538
2.5ArgThr: 2.5 ± 0.454
2.586ArgVal: 2.586 ± 0.594
1.035ArgTrp: 1.035 ± 0.32
1.379ArgTyr: 1.379 ± 0.287
0.0ArgXaa: 0.0 ± 0.0
Ser
4.742SerAla: 4.742 ± 0.703
0.69SerCys: 0.69 ± 0.319
4.742SerAsp: 4.742 ± 0.426
3.362SerGlu: 3.362 ± 0.692
3.018SerPhe: 3.018 ± 0.44
6.035SerGly: 6.035 ± 0.72
1.983SerHis: 1.983 ± 0.344
2.673SerIle: 2.673 ± 0.543
2.931SerLys: 2.931 ± 0.496
3.793SerLeu: 3.793 ± 0.571
1.121SerMet: 1.121 ± 0.264
3.19SerAsn: 3.19 ± 0.536
2.845SerPro: 2.845 ± 0.522
1.897SerGln: 1.897 ± 0.49
3.621SerArg: 3.621 ± 0.51
3.793SerSer: 3.793 ± 0.558
2.845SerThr: 2.845 ± 0.352
4.138SerVal: 4.138 ± 0.424
0.862SerTrp: 0.862 ± 0.238
2.845SerTyr: 2.845 ± 0.541
0.0SerXaa: 0.0 ± 0.0
Thr
3.88ThrAla: 3.88 ± 0.772
0.431ThrCys: 0.431 ± 0.183
3.707ThrAsp: 3.707 ± 0.659
5.087ThrGlu: 5.087 ± 0.617
2.586ThrPhe: 2.586 ± 0.402
5.863ThrGly: 5.863 ± 0.593
0.948ThrHis: 0.948 ± 0.297
3.966ThrIle: 3.966 ± 0.612
3.19ThrLys: 3.19 ± 0.502
5.345ThrLeu: 5.345 ± 0.606
1.638ThrMet: 1.638 ± 0.322
2.155ThrAsn: 2.155 ± 0.578
2.759ThrPro: 2.759 ± 0.329
2.586ThrGln: 2.586 ± 0.583
2.414ThrArg: 2.414 ± 0.501
2.242ThrSer: 2.242 ± 0.451
3.535ThrThr: 3.535 ± 0.652
4.311ThrVal: 4.311 ± 0.527
0.604ThrTrp: 0.604 ± 0.225
1.466ThrTyr: 1.466 ± 0.303
0.0ThrXaa: 0.0 ± 0.0
Val
5.087ValAla: 5.087 ± 0.74
0.345ValCys: 0.345 ± 0.186
3.621ValAsp: 3.621 ± 0.545
4.483ValGlu: 4.483 ± 0.764
2.069ValPhe: 2.069 ± 0.506
6.121ValGly: 6.121 ± 0.668
1.724ValHis: 1.724 ± 0.651
3.19ValIle: 3.19 ± 0.479
5.0ValLys: 5.0 ± 0.619
5.087ValLeu: 5.087 ± 0.447
2.155ValMet: 2.155 ± 0.499
3.449ValAsn: 3.449 ± 0.657
3.19ValPro: 3.19 ± 0.54
3.104ValGln: 3.104 ± 0.482
4.138ValArg: 4.138 ± 0.593
4.914ValSer: 4.914 ± 0.633
4.569ValThr: 4.569 ± 0.641
5.345ValVal: 5.345 ± 0.896
0.776ValTrp: 0.776 ± 0.245
2.759ValTyr: 2.759 ± 0.5
0.0ValXaa: 0.0 ± 0.0
Trp
0.431TrpAla: 0.431 ± 0.145
0.086TrpCys: 0.086 ± 0.084
0.69TrpAsp: 0.69 ± 0.249
1.207TrpGlu: 1.207 ± 0.295
0.776TrpPhe: 0.776 ± 0.273
0.948TrpGly: 0.948 ± 0.277
0.431TrpHis: 0.431 ± 0.222
0.431TrpIle: 0.431 ± 0.223
1.552TrpLys: 1.552 ± 0.336
2.242TrpLeu: 2.242 ± 0.491
0.172TrpMet: 0.172 ± 0.121
0.948TrpAsn: 0.948 ± 0.265
0.345TrpPro: 0.345 ± 0.187
0.69TrpGln: 0.69 ± 0.249
0.776TrpArg: 0.776 ± 0.241
0.776TrpSer: 0.776 ± 0.422
0.862TrpThr: 0.862 ± 0.291
1.035TrpVal: 1.035 ± 0.289
0.086TrpTrp: 0.086 ± 0.085
0.345TrpTyr: 0.345 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.707TyrAla: 3.707 ± 0.579
0.345TyrCys: 0.345 ± 0.162
2.242TyrAsp: 2.242 ± 0.422
1.638TyrGlu: 1.638 ± 0.476
1.121TyrPhe: 1.121 ± 0.247
3.19TyrGly: 3.19 ± 0.573
0.431TyrHis: 0.431 ± 0.2
1.638TyrIle: 1.638 ± 0.47
2.242TyrLys: 2.242 ± 0.39
2.069TyrLeu: 2.069 ± 0.305
0.948TyrMet: 0.948 ± 0.249
1.811TyrAsn: 1.811 ± 0.41
1.293TyrPro: 1.293 ± 0.342
1.466TyrGln: 1.466 ± 0.514
2.414TyrArg: 2.414 ± 0.456
2.242TyrSer: 2.242 ± 0.374
1.983TyrThr: 1.983 ± 0.306
2.5TyrVal: 2.5 ± 0.402
0.604TyrTrp: 0.604 ± 0.199
1.552TyrTyr: 1.552 ± 0.447
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (11600 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski