Thelazia callipaeda (Oriental eyeworm) (Parasitic nematode)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Spiruromorpha; Thelazioidea; Thelaziidae; Thelazia

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10885 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N5DBB6|A0A0N5DBB6_THECL TPR_REGION domain-containing protein OS=Thelazia callipaeda OX=103827 GN=TCLT_LOCUS10467 PE=4 SV=1
MM1 pKa = 7.69SSKK4 pKa = 10.35CDD6 pKa = 3.43HH7 pKa = 6.86EE8 pKa = 4.57YY9 pKa = 11.17KK10 pKa = 10.42SDD12 pKa = 5.09GDD14 pKa = 4.21DD15 pKa = 4.78QDD17 pKa = 4.88DD18 pKa = 4.49DD19 pKa = 5.16YY20 pKa = 11.87YY21 pKa = 12.08NHH23 pKa = 7.6DD24 pKa = 3.9GDD26 pKa = 4.17GDD28 pKa = 4.22GDD30 pKa = 3.92VMVIVMTTMMMITTIIIEE48 pKa = 4.29EE49 pKa = 4.43FSQVSIKK56 pKa = 10.48DD57 pKa = 3.14INFILVLTTCPSPFTDD73 pKa = 3.39QQHH76 pKa = 5.63AQIFTTAMNIIYY88 pKa = 10.31VMNNINTQTSLL99 pKa = 3.39

Molecular weight: 11.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N5D0A8|A0A0N5D0A8_THECL Uncharacterized protein OS=Thelazia callipaeda OX=103827 GN=TCLT_LOCUS6212 PE=4 SV=2
MM1 pKa = 7.11EE2 pKa = 5.47RR3 pKa = 11.84KK4 pKa = 10.15GGVCSSPLLYY14 pKa = 10.29IPGVCVEE21 pKa = 4.1SHH23 pKa = 6.22NLSSSGRR30 pKa = 11.84SFVQQRR36 pKa = 11.84RR37 pKa = 11.84FSRR40 pKa = 11.84RR41 pKa = 11.84LRR43 pKa = 11.84RR44 pKa = 11.84AIEE47 pKa = 4.49AIFITDD53 pKa = 3.29KK54 pKa = 11.11FGLITCGRR62 pKa = 11.84DD63 pKa = 3.03ASALSRR69 pKa = 11.84KK70 pKa = 9.58DD71 pKa = 3.29LSYY74 pKa = 10.85SQQRR78 pKa = 11.84QISAFAA84 pKa = 4.1

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10885

0

10885

4428208

29

7297

406.8

46.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.026 ± 0.017

2.341 ± 0.019

5.316 ± 0.019

6.456 ± 0.032

4.445 ± 0.018

4.906 ± 0.025

2.468 ± 0.01

6.494 ± 0.021

6.28 ± 0.025

9.48 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.009

5.119 ± 0.018

4.161 ± 0.024

4.099 ± 0.021

5.576 ± 0.017

8.486 ± 0.03

5.358 ± 0.019

6.155 ± 0.017

1.121 ± 0.008

3.294 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski