Streptococcus satellite phage Javan600
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZRJ5|A0A4D5ZRJ5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan600 OX=2558769 GN=JavanS600_0002 PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.14 IKK4 pKa = 10.81 LFTRR8 pKa = 11.84 EE9 pKa = 3.95 HH10 pKa = 6.52 VSDD13 pKa = 5.89 GIHH16 pKa = 5.4 EE17 pKa = 4.29 TLGFEE22 pKa = 4.81 KK23 pKa = 10.52 FRR25 pKa = 11.84 IEE27 pKa = 3.85 NDD29 pKa = 3.29 VEE31 pKa = 4.2 FEE33 pKa = 4.01 TRR35 pKa = 11.84 INDD38 pKa = 3.32 FMIDD42 pKa = 3.44 KK43 pKa = 10.52 NVVSVQSLKK52 pKa = 11.17 DD53 pKa = 3.7 SVFVTYY59 pKa = 11.13 ADD61 pKa = 3.35
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.006
IPC2_protein 5.016
IPC_protein 4.825
Toseland 4.724
ProMoST 4.762
Dawson 4.8
Bjellqvist 5.003
Wikipedia 4.685
Rodwell 4.711
Grimsley 4.647
Solomon 4.8
Lehninger 4.762
Nozaki 4.927
DTASelect 5.08
Thurlkill 4.736
EMBOSS 4.711
Sillero 4.978
Patrickios 4.406
IPC_peptide 4.813
IPC2_peptide 4.978
IPC2.peptide.svr19 4.964
Protein with the highest isoelectric point:
>tr|A0A4D5ZRK7|A0A4D5ZRK7_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan600 OX=2558769 GN=JavanS600_0012 PE=3 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.57 RR3 pKa = 11.84 LGYY6 pKa = 10.64 LSAFKK11 pKa = 10.61 LLKK14 pKa = 10.5 KK15 pKa = 10.45 VDD17 pKa = 3.58 IKK19 pKa = 10.96 RR20 pKa = 11.84 PNYY23 pKa = 10.39 GKK25 pKa = 9.06 MLEE28 pKa = 4.25 NVGINAIDD36 pKa = 3.23 RR37 pKa = 4.1
Molecular weight: 4.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 9.663
IPC_protein 9.75
Toseland 10.496
ProMoST 10.262
Dawson 10.599
Bjellqvist 10.204
Wikipedia 10.73
Rodwell 11.228
Grimsley 10.643
Solomon 10.643
Lehninger 10.628
Nozaki 10.452
DTASelect 10.218
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.511
Patrickios 11.023
IPC_peptide 10.643
IPC2_peptide 8.697
IPC2.peptide.svr19 8.672
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
2138
37
502
178.2
20.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.034 ± 0.403
0.421 ± 0.133
5.706 ± 0.442
7.343 ± 0.788
3.648 ± 0.565
5.332 ± 0.238
1.731 ± 0.388
6.221 ± 0.452
9.916 ± 0.55
9.121 ± 0.761
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.292 ± 0.278
6.829 ± 0.631
3.601 ± 0.77
3.742 ± 1.097
4.163 ± 0.41
5.847 ± 0.667
7.016 ± 0.893
5.753 ± 0.488
0.935 ± 0.154
4.35 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here