Sporomusa termitida
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4716 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A517DR56|A0A517DR56_9FIRM TRAP-T-associated universal stress protein TeaD OS=Sporomusa termitida OX=2377 GN=teaD PE=3 SV=1
MM1 pKa = 7.11 STTSVSNYY9 pKa = 6.93 WKK11 pKa = 9.44 TPTTTAADD19 pKa = 3.93 TTSTTGTSSLDD30 pKa = 3.35 FNDD33 pKa = 4.22 FVEE36 pKa = 4.83 LLATEE41 pKa = 5.45 LRR43 pKa = 11.84 YY44 pKa = 9.49 QDD46 pKa = 3.73 PQDD49 pKa = 3.93 PVSSTEE55 pKa = 3.86 YY56 pKa = 10.25 VAQLAQFGTLDD67 pKa = 3.4 KK68 pKa = 11.32 LNTIGNSVDD77 pKa = 3.65 AYY79 pKa = 10.25 QAYY82 pKa = 9.98 GLIGKK87 pKa = 8.94 SVTYY91 pKa = 8.94 ATTDD95 pKa = 3.2 AAGAAASATGTVDD108 pKa = 3.2 SVSIKK113 pKa = 10.84 SGTAYY118 pKa = 11.03 LNIGSMQITLSSVSQVAEE136 pKa = 3.97 ASSS139 pKa = 3.21
Molecular weight: 14.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.617
ProMoST 4.024
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 0.769
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A517DZF0|A0A517DZF0_9FIRM PhnA: putative alkylphosphonate utilization operon protein PhnA OS=Sporomusa termitida OX=2377 GN=SPTER_41620 PE=3 SV=1
MM1 pKa = 7.26 KK2 pKa = 9.38 QTTKK6 pKa = 10.28 PAPTAQINKK15 pKa = 9.83 KK16 pKa = 8.99 GRR18 pKa = 11.84 PWAAWLIIVAGSGLLVLLMMFSITRR43 pKa = 11.84 GAAAIPFFAVGDD55 pKa = 3.75 ALVRR59 pKa = 11.84 FDD61 pKa = 5.49 AKK63 pKa = 9.86 NTQHH67 pKa = 7.32 LIVLDD72 pKa = 3.77 LRR74 pKa = 11.84 LPRR77 pKa = 11.84 VIASALVGAALAVAGAVMQGTTRR100 pKa = 11.84 NPLADD105 pKa = 3.91 SGLMGLNAGAGFALSICFAFFPRR128 pKa = 11.84 LGYY131 pKa = 8.41 MQLILFSFLGAALGAALVGGIASLRR156 pKa = 11.84 RR157 pKa = 11.84 GGATPMRR164 pKa = 11.84 LVLAGAAVSALLAALSQGIALYY186 pKa = 10.55 FDD188 pKa = 3.79 VAQDD192 pKa = 3.12 IMFWTTGGVAAASWEE207 pKa = 4.43 QIRR210 pKa = 11.84 IMAPWILGALLGSIALARR228 pKa = 11.84 SVSLLSLGQDD238 pKa = 3.39 VAKK241 pKa = 10.85 GLGLNTAVGNGLCFLIVLILAGASVSVVGAVGFVGLIIPHH281 pKa = 6.58 LARR284 pKa = 11.84 YY285 pKa = 8.2 FVGVDD290 pKa = 3.63 YY291 pKa = 10.84 RR292 pKa = 11.84 WVIPSSAVLGALLLVLADD310 pKa = 4.27 LGARR314 pKa = 11.84 TLNPPFEE321 pKa = 4.43 TPIGALIGLIGVPCFLYY338 pKa = 10.58 LARR341 pKa = 11.84 QQRR344 pKa = 11.84 MGLL347 pKa = 3.57
Molecular weight: 35.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.663
IPC_protein 10.496
Toseland 10.482
ProMoST 10.233
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.804
Grimsley 10.701
Solomon 10.73
Lehninger 10.687
Nozaki 10.496
DTASelect 10.365
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.57
Patrickios 10.496
IPC_peptide 10.73
IPC2_peptide 9.516
IPC2.peptide.svr19 8.509
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4716
0
4716
1486555
34
7221
315.2
34.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.234 ± 0.058
1.223 ± 0.018
4.762 ± 0.036
5.864 ± 0.043
3.74 ± 0.032
7.994 ± 0.128
1.805 ± 0.017
7.082 ± 0.034
5.266 ± 0.044
10.035 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.544 ± 0.02
3.859 ± 0.033
4.142 ± 0.031
4.028 ± 0.035
4.899 ± 0.036
5.237 ± 0.037
5.661 ± 0.121
7.355 ± 0.037
1.019 ± 0.017
3.252 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here