Frankia sp. EUN1f

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia; unclassified Frankia

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3DDP7|D3DDP7_9ACTN Uncharacterized protein (Fragment) OS=Frankia sp. EUN1f OX=102897 GN=FrEUN1fDRAFT_7919 PE=4 SV=1
MM1 pKa = 7.81ASPTAYY7 pKa = 10.44LAFGVALGGGDD18 pKa = 4.08DD19 pKa = 4.25GFDD22 pKa = 3.21GLVLPEE28 pKa = 4.52GEE30 pKa = 4.12YY31 pKa = 11.39GMRR34 pKa = 11.84LLPEE38 pKa = 4.26WLHH41 pKa = 6.39DD42 pKa = 3.95APAGDD47 pKa = 4.95EE48 pKa = 3.9AAIWDD53 pKa = 4.55RR54 pKa = 11.84LLPAYY59 pKa = 10.25DD60 pKa = 3.8VDD62 pKa = 4.39TPDD65 pKa = 3.61AAPVEE70 pKa = 4.34LTTYY74 pKa = 10.21GYY76 pKa = 11.05EE77 pKa = 4.1FTALLLHH84 pKa = 6.06TRR86 pKa = 11.84GTLVSAIDD94 pKa = 3.35WGDD97 pKa = 3.63SVVSAEE103 pKa = 4.16LLARR107 pKa = 11.84PAADD111 pKa = 3.34LAAPVIAALATLGLTAKK128 pKa = 8.33EE129 pKa = 3.89APGWVLATTYY139 pKa = 11.05GG140 pKa = 3.34

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3D2L2|D3D2L2_9ACTN Uncharacterized protein OS=Frankia sp. EUN1f OX=102897 GN=FrEUN1fDRAFT_4033 PE=4 SV=1
MM1 pKa = 7.22ATGGRR6 pKa = 11.84ARR8 pKa = 11.84YY9 pKa = 8.34VAGRR13 pKa = 11.84RR14 pKa = 11.84GITGPRR20 pKa = 11.84GLTGRR25 pKa = 11.84EE26 pKa = 3.71LSGQGVQAIRR36 pKa = 11.84RR37 pKa = 11.84IGLRR41 pKa = 11.84RR42 pKa = 11.84LARR45 pKa = 11.84LEE47 pKa = 3.77VSRR50 pKa = 11.84LGLTRR55 pKa = 11.84LRR57 pKa = 11.84VARR60 pKa = 11.84LRR62 pKa = 11.84LSLLWVSRR70 pKa = 11.84LGLTRR75 pKa = 11.84LRR77 pKa = 11.84VARR80 pKa = 11.84LRR82 pKa = 11.84LSLLWVTRR90 pKa = 11.84LWVTRR95 pKa = 11.84LWLSRR100 pKa = 11.84LSILPRR106 pKa = 11.84RR107 pKa = 11.84ITARR111 pKa = 11.84RR112 pKa = 11.84VAAVLISTAHH122 pKa = 5.23GTPTRR127 pKa = 11.84IRR129 pKa = 11.84RR130 pKa = 11.84SAAIGISRR138 pKa = 11.84LRR140 pKa = 11.84IVRR143 pKa = 11.84VRR145 pKa = 11.84LRR147 pKa = 11.84LTT149 pKa = 3.22

Molecular weight: 16.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8160

0

8160

2647754

30

6652

324.5

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.108 ± 0.044

0.791 ± 0.008

6.108 ± 0.021

5.08 ± 0.022

2.65 ± 0.015

9.743 ± 0.028

2.154 ± 0.011

3.429 ± 0.019

1.404 ± 0.015

10.029 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.621 ± 0.01

1.716 ± 0.014

6.53 ± 0.028

2.665 ± 0.013

8.547 ± 0.033

5.41 ± 0.021

6.133 ± 0.025

8.593 ± 0.025

1.456 ± 0.012

1.832 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski