Enterococcus asini ATCC 700915

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus asini

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R2PXA6|R2PXA6_9ENTE Uncharacterized protein OS=Enterococcus asini ATCC 700915 OX=1158606 GN=UAS_00631 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.09LIKK5 pKa = 10.47SILFGTAVLAGVAAFGSQSDD25 pKa = 3.6AAEE28 pKa = 4.22IEE30 pKa = 4.42HH31 pKa = 6.43TVTSSDD37 pKa = 4.0TLWGLSMKK45 pKa = 10.72YY46 pKa = 10.17YY47 pKa = 10.93GSDD50 pKa = 4.37DD51 pKa = 4.24YY52 pKa = 11.53IQTIANDD59 pKa = 3.79NNIEE63 pKa = 4.11NADD66 pKa = 3.89MIYY69 pKa = 10.68DD70 pKa = 4.03GEE72 pKa = 4.31VLKK75 pKa = 11.01INTDD79 pKa = 3.36SPAATAAQTEE89 pKa = 4.58TPKK92 pKa = 10.92AVAEE96 pKa = 4.5TTTEE100 pKa = 3.8AAPAEE105 pKa = 4.17TSEE108 pKa = 4.31PVTTTTEE115 pKa = 3.73EE116 pKa = 3.96ANYY119 pKa = 10.58SGTTMYY125 pKa = 10.07MEE127 pKa = 4.56ATAYY131 pKa = 10.85SSDD134 pKa = 3.62PADD137 pKa = 3.65ALGGGTVTATGQNLLEE153 pKa = 4.5NPMAVAVDD161 pKa = 3.82PSVIPLGTHH170 pKa = 7.02LYY172 pKa = 9.73VEE174 pKa = 5.63GYY176 pKa = 10.01GEE178 pKa = 4.76AYY180 pKa = 10.25AVDD183 pKa = 3.91TGSAIQGNIIDD194 pKa = 4.06VHH196 pKa = 6.01FSSYY200 pKa = 9.73DD201 pKa = 3.16QCVAWGRR208 pKa = 11.84RR209 pKa = 11.84QVKK212 pKa = 7.72VTILDD217 pKa = 3.46

Molecular weight: 22.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R2S995|R2S995_9ENTE 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase OS=Enterococcus asini ATCC 700915 OX=1158606 GN=UAS_00520 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.54NGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.85GRR39 pKa = 11.84KK40 pKa = 8.75VLAAA44 pKa = 4.31

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2389

0

2389

736464

22

1672

308.3

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.297 ± 0.058

0.683 ± 0.015

5.376 ± 0.058

7.2 ± 0.049

4.452 ± 0.04

7.03 ± 0.048

1.861 ± 0.019

6.568 ± 0.044

6.112 ± 0.039

10.335 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.024

3.982 ± 0.034

3.781 ± 0.028

4.594 ± 0.049

4.142 ± 0.039

5.459 ± 0.044

5.837 ± 0.037

7.097 ± 0.038

0.992 ± 0.016

3.609 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski