Abalone herpesvirus (isolate Abalone/Australia/Victoria/2009) (AbHV)
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 112 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K4JUE5|K4JUE5_ABHV Uncharacterized protein OS=Abalone herpesvirus (isolate Abalone/Australia/Victoria/2009) OX=1241371 GN=AbHV_ORF19 PE=4 SV=1
MM1 pKa = 8.12 DD2 pKa = 5.86 DD3 pKa = 4.46 KK4 pKa = 11.63 SSGMQAAALATEE16 pKa = 4.94 DD17 pKa = 5.05 DD18 pKa = 4.21 DD19 pKa = 6.99 DD20 pKa = 4.74 GVSSLVDD27 pKa = 3.94 MINSGALSLEE37 pKa = 4.52 VEE39 pKa = 4.34 RR40 pKa = 11.84 QMKK43 pKa = 10.21 RR44 pKa = 11.84 DD45 pKa = 3.42 GEE47 pKa = 4.38 RR48 pKa = 11.84 NLLFYY53 pKa = 10.89 QEE55 pKa = 4.14 VMSATFDD62 pKa = 3.83 RR63 pKa = 11.84 DD64 pKa = 3.32 LLRR67 pKa = 11.84 SKK69 pKa = 10.41 IFPEE73 pKa = 4.49 DD74 pKa = 3.58 ASKK77 pKa = 10.65 GPCMRR82 pKa = 11.84 MKK84 pKa = 10.47 QSLYY88 pKa = 10.81 KK89 pKa = 9.99 EE90 pKa = 3.82 ASKK93 pKa = 10.64 PIVGEE98 pKa = 4.0 YY99 pKa = 10.57 AFEE102 pKa = 4.47 SDD104 pKa = 4.25 PVFSSASTAHH114 pKa = 6.12 LRR116 pKa = 11.84 LVEE119 pKa = 3.96 KK120 pKa = 10.85 SKK122 pKa = 10.73 ILSKK126 pKa = 10.54 DD127 pKa = 2.77 IPEE130 pKa = 4.22 IVLDD134 pKa = 4.04 YY135 pKa = 11.11 NQDD138 pKa = 3.35 PSVISVDD145 pKa = 3.59 EE146 pKa = 4.44 SNMIVTDD153 pKa = 3.16 VHH155 pKa = 6.87 HH156 pKa = 6.42 PQEE159 pKa = 4.11 NQTRR163 pKa = 11.84 IFFQLDD169 pKa = 2.9 NGYY172 pKa = 9.6 VLSIPTVNPWSEE184 pKa = 3.69 RR185 pKa = 11.84 IYY187 pKa = 10.32 RR188 pKa = 11.84 YY189 pKa = 8.64 TITSVPQDD197 pKa = 3.66 KK198 pKa = 10.65 EE199 pKa = 3.93 LLVYY203 pKa = 10.92 DD204 pKa = 3.9 NLEE207 pKa = 4.24 DD208 pKa = 4.06 VFKK211 pKa = 10.96 NYY213 pKa = 10.06 PNYY216 pKa = 10.56 AEE218 pKa = 4.95 KK219 pKa = 10.96 LFTNAPDD226 pKa = 5.4 DD227 pKa = 5.31 DD228 pKa = 6.56 DD229 pKa = 6.93 DD230 pKa = 6.33 DD231 pKa = 5.21 EE232 pKa = 5.37 EE233 pKa = 5.64 GGPEE237 pKa = 4.1 QYY239 pKa = 11.09 LKK241 pKa = 10.85 LDD243 pKa = 3.5 RR244 pKa = 11.84 SFLGVSGVSDD254 pKa = 3.89 GSDD257 pKa = 3.36 LEE259 pKa = 4.68 VVARR263 pKa = 11.84 YY264 pKa = 8.45 HH265 pKa = 7.5 IPMEE269 pKa = 4.61 LNPLQYY275 pKa = 11.29 VMHH278 pKa = 6.97 KK279 pKa = 10.01 PNLYY283 pKa = 10.53 LFTGKK288 pKa = 9.97 DD289 pKa = 3.22 QEE291 pKa = 4.6 GNNNCLYY298 pKa = 11.01 ALNTEE303 pKa = 4.24 LVEE306 pKa = 4.73 GEE308 pKa = 4.38 GKK310 pKa = 9.59 PWIAPWTKK318 pKa = 10.14 VKK320 pKa = 10.32 IYY322 pKa = 10.65 DD323 pKa = 3.47 SEE325 pKa = 4.95 TYY327 pKa = 10.94 VDD329 pKa = 4.01 LTDD332 pKa = 4.72 PNTLTVFEE340 pKa = 5.44 DD341 pKa = 3.93 EE342 pKa = 4.55 VPSYY346 pKa = 11.06 RR347 pKa = 11.84 LTIEE351 pKa = 3.76 VSEE354 pKa = 4.29 GSNIGVLSVPDD365 pKa = 3.91 YY366 pKa = 7.96 PTYY369 pKa = 10.46 DD370 pKa = 3.41 IPSLTLIEE378 pKa = 4.23 EE379 pKa = 4.29 VNDD382 pKa = 3.5 IVLEE386 pKa = 4.16 AAEE389 pKa = 4.64 EE390 pKa = 4.07 EE391 pKa = 4.35 DD392 pKa = 6.18 DD393 pKa = 4.39 EE394 pKa = 5.26 DD395 pKa = 4.15
Molecular weight: 44.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.179
IPC2_protein 4.075
IPC_protein 4.075
Toseland 3.872
ProMoST 4.177
Dawson 4.037
Bjellqvist 4.202
Wikipedia 3.935
Rodwell 3.897
Grimsley 3.783
Solomon 4.037
Lehninger 3.986
Nozaki 4.139
DTASelect 4.342
Thurlkill 3.897
EMBOSS 3.948
Sillero 4.177
Patrickios 3.401
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.101
Protein with the highest isoelectric point:
>tr|K4JYI3|K4JYI3_ABHV Uncharacterized protein OS=Abalone herpesvirus (isolate Abalone/Australia/Victoria/2009) OX=1241371 GN=AbHV_ORF66 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.29 FVTRR6 pKa = 11.84 KK7 pKa = 10.0 RR8 pKa = 11.84 LVTKK12 pKa = 10.14 MLLTHH17 pKa = 6.76 KK18 pKa = 10.07 FKK20 pKa = 11.12 LVTRR24 pKa = 11.84 TGLATKK30 pKa = 10.11 KK31 pKa = 10.61 YY32 pKa = 10.1 LALKK36 pKa = 10.12 MNDD39 pKa = 2.99 TWGKK43 pKa = 10.09 DD44 pKa = 3.15 ALSRR48 pKa = 11.84 AEE50 pKa = 3.61 INYY53 pKa = 9.61 VNEE56 pKa = 3.57 NDD58 pKa = 3.5 TWGKK62 pKa = 9.24 NAPTLQKK69 pKa = 10.41 NLRR72 pKa = 11.84 SAVFLVPTSIVHH84 pKa = 5.75 AQHH87 pKa = 6.46 PQRR90 pKa = 11.84 KK91 pKa = 7.15 RR92 pKa = 11.84 TEE94 pKa = 3.71 ARR96 pKa = 11.84 LPPSLNLQRR105 pKa = 11.84 TLTDD109 pKa = 2.82 SKK111 pKa = 10.3 MYY113 pKa = 9.94 PSLKK117 pKa = 9.78 IILRR121 pKa = 11.84 RR122 pKa = 11.84 KK123 pKa = 8.31 RR124 pKa = 11.84 TVATLLPSLKK134 pKa = 10.12 KK135 pKa = 10.51 KK136 pKa = 10.21 LWSGLCWTT144 pKa = 5.04
Molecular weight: 16.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 10.101
IPC_protein 10.994
Toseland 11.33
ProMoST 11.286
Dawson 11.374
Bjellqvist 11.14
Wikipedia 11.637
Rodwell 11.681
Grimsley 11.403
Solomon 11.608
Lehninger 11.564
Nozaki 11.316
DTASelect 11.14
Thurlkill 11.316
EMBOSS 11.754
Sillero 11.316
Patrickios 11.403
IPC_peptide 11.623
IPC2_peptide 10.189
IPC2.peptide.svr19 8.472
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
112
0
112
56122
79
1926
501.1
57.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.7 ± 0.13
1.937 ± 0.118
5.928 ± 0.13
8.025 ± 0.247
4.321 ± 0.095
4.994 ± 0.12
1.994 ± 0.101
5.675 ± 0.126
7.658 ± 0.196
8.269 ± 0.195
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.067 ± 0.096
5.023 ± 0.119
4.572 ± 0.18
3.304 ± 0.09
5.399 ± 0.168
6.976 ± 0.15
5.958 ± 0.144
6.971 ± 0.1
0.798 ± 0.043
3.43 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here