Trinickia symbiotica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Trinickia

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5982 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N7WKB9|A0A2N7WKB9_9BURK MBL fold metallo-hydrolase OS=Trinickia symbiotica OX=863227 GN=C0Z20_30580 PE=4 SV=1
MM1 pKa = 8.08DD2 pKa = 4.31AQEE5 pKa = 4.8LGVLEE10 pKa = 4.87KK11 pKa = 10.28FTGEE15 pKa = 3.94NFVSSFTGGPDD26 pKa = 3.22PSNWHH31 pKa = 5.16QAQVLQDD38 pKa = 3.79AFNQLMNAVEE48 pKa = 4.27VRR50 pKa = 11.84LVAQGPLASYY60 pKa = 10.84LSGVGFNYY68 pKa = 9.13DD69 pKa = 3.04TDD71 pKa = 4.22SLVGSVDD78 pKa = 3.5LTALAEE84 pKa = 4.12ALINATPAGQLAAEE98 pKa = 5.45GYY100 pKa = 7.67WSSVAPMVGNLCSEE114 pKa = 4.82LGLGASAYY122 pKa = 7.54QTAFQGPFSSLNLPFSLDD140 pKa = 3.67EE141 pKa = 4.0IVQGNVFLGNGTNTVAVAHH160 pKa = 5.83TAGVPHH166 pKa = 6.65YY167 pKa = 9.9FDD169 pKa = 4.47GGPGVRR175 pKa = 11.84YY176 pKa = 9.36EE177 pKa = 4.27GSSGSGDD184 pKa = 3.02VFVFNQGYY192 pKa = 8.2GQLEE196 pKa = 4.05INEE199 pKa = 4.38FDD201 pKa = 3.37WNASNNVLRR210 pKa = 11.84LGAGITPDD218 pKa = 3.43AVKK221 pKa = 9.58VTLDD225 pKa = 3.73DD226 pKa = 4.0NQDD229 pKa = 3.01ILLTIGSNGDD239 pKa = 3.56QIKK242 pKa = 10.61LDD244 pKa = 3.85SEE246 pKa = 4.75GTGDD250 pKa = 4.02LRR252 pKa = 11.84WGVEE256 pKa = 3.89QVQFADD262 pKa = 4.13GAAWTQSQLIASARR276 pKa = 11.84NIQGTAASEE285 pKa = 4.05TLYY288 pKa = 9.62GTNGADD294 pKa = 3.2TFDD297 pKa = 4.52GKK299 pKa = 11.36GSTPYY304 pKa = 10.28SGSDD308 pKa = 3.36VEE310 pKa = 5.08IGNGGNDD317 pKa = 2.89TFIFNEE323 pKa = 5.21GYY325 pKa = 10.84GKK327 pKa = 8.74LTINEE332 pKa = 4.04YY333 pKa = 10.85DD334 pKa = 3.62YY335 pKa = 11.63GASPEE340 pKa = 4.15NVLQLGAGITPDD352 pKa = 3.32QVVVSVDD359 pKa = 3.04RR360 pKa = 11.84SQNIVLTIGTNGDD373 pKa = 3.05QVMLNRR379 pKa = 11.84EE380 pKa = 4.07ATGDD384 pKa = 3.39ARR386 pKa = 11.84EE387 pKa = 4.45GVQQIQFADD396 pKa = 3.94GTIWTQSQLLGMLATIDD413 pKa = 4.06GTPQDD418 pKa = 3.78DD419 pKa = 3.8TLYY422 pKa = 9.41GTGGANVFDD431 pKa = 4.41GKK433 pKa = 10.78GGYY436 pKa = 9.31DD437 pKa = 3.29VEE439 pKa = 4.63VGNGGNDD446 pKa = 3.13TYY448 pKa = 11.6VFNSGYY454 pKa = 8.15GTLVIKK460 pKa = 10.72DD461 pKa = 3.78SGSGVLDD468 pKa = 4.68LGTGITPSEE477 pKa = 4.19VKK479 pKa = 10.43ISTDD483 pKa = 3.25YY484 pKa = 11.55SGDD487 pKa = 3.21IVLTIGTEE495 pKa = 4.15GDD497 pKa = 3.42QVMLSGMTRR506 pKa = 11.84GASSSIQQVLFADD519 pKa = 4.59GTSWTAAQILAWSSDD534 pKa = 3.31IQGTTGNDD542 pKa = 3.33DD543 pKa = 4.12LYY545 pKa = 10.5GTSGDD550 pKa = 4.24DD551 pKa = 3.49VFDD554 pKa = 4.25GKK556 pKa = 11.08GGNDD560 pKa = 3.49YY561 pKa = 11.3EE562 pKa = 4.6SGRR565 pKa = 11.84GGNDD569 pKa = 2.83TFIFNQGYY577 pKa = 7.93GHH579 pKa = 7.37LEE581 pKa = 3.44ISEE584 pKa = 4.5VNGSAGFDD592 pKa = 3.41SVLEE596 pKa = 4.29LGSGITPSAVTIAYY610 pKa = 8.69GPNQSLVLTDD620 pKa = 4.41GNDD623 pKa = 3.24QITLDD628 pKa = 3.76GMANGSGRR636 pKa = 11.84DD637 pKa = 3.73GVQTVQFADD646 pKa = 3.9GTVWTASQMLASIGEE661 pKa = 4.06IRR663 pKa = 11.84GTAGDD668 pKa = 3.91DD669 pKa = 3.49QLFGTSGANLFDD681 pKa = 4.25GGGGNDD687 pKa = 3.82YY688 pKa = 11.36EE689 pKa = 6.34DD690 pKa = 4.83GDD692 pKa = 4.19GGNDD696 pKa = 3.06TFIFNRR702 pKa = 11.84GYY704 pKa = 8.6GTLEE708 pKa = 3.72IVEE711 pKa = 4.3QGAATTTGTLLLGPGILPSSVTVSLAGDD739 pKa = 3.88SVLLTIGSNGDD750 pKa = 3.66QVKK753 pKa = 10.4LDD755 pKa = 3.46YY756 pKa = 8.43MTDD759 pKa = 3.14RR760 pKa = 11.84GSNFGVRR767 pKa = 11.84DD768 pKa = 3.54VQFADD773 pKa = 3.73GTVWTASQILASLSDD788 pKa = 3.54VSGTAKK794 pKa = 10.36DD795 pKa = 3.8DD796 pKa = 4.62SIGGTSDD803 pKa = 3.27PSVIDD808 pKa = 3.75GKK810 pKa = 11.08GGNDD814 pKa = 3.14VAGGQGNNDD823 pKa = 3.27TFVFKK828 pKa = 10.72AGYY831 pKa = 9.4GHH833 pKa = 6.68LTVYY837 pKa = 10.11EE838 pKa = 4.03PQAANGVTNVLLFGPDD854 pKa = 2.87IDD856 pKa = 4.12EE857 pKa = 4.52SFVTVKK863 pKa = 10.91EE864 pKa = 4.01NMQDD868 pKa = 3.44AVVLTDD874 pKa = 5.28GIAGDD879 pKa = 4.13EE880 pKa = 4.23VTLVNQAYY888 pKa = 8.88EE889 pKa = 4.27FQGLQYY895 pKa = 11.13GGVQEE900 pKa = 4.31VQFADD905 pKa = 3.46GTMWTAQQLLQRR917 pKa = 11.84ATQGTPGDD925 pKa = 3.98DD926 pKa = 3.49VLIGGWQASLLDD938 pKa = 3.9GKK940 pKa = 10.9GGNDD944 pKa = 3.49YY945 pKa = 10.96EE946 pKa = 5.53AGGGDD951 pKa = 3.35GDD953 pKa = 3.83TFVFNAGYY961 pKa = 10.35GHH963 pKa = 6.7LQINEE968 pKa = 4.32YY969 pKa = 9.85QWGTANNVLQLGQGISEE986 pKa = 4.31SSISASVTRR995 pKa = 11.84GDD997 pKa = 5.25AIVLSDD1003 pKa = 4.41GTPGDD1008 pKa = 3.79QIVLDD1013 pKa = 4.44GEE1015 pKa = 4.33FDD1017 pKa = 3.56DD1018 pKa = 5.2ARR1020 pKa = 11.84YY1021 pKa = 10.21GGTSRR1026 pKa = 11.84GIQEE1030 pKa = 3.89VQFADD1035 pKa = 3.51GTTWSVQQLVQLAQTRR1051 pKa = 11.84TGTPGDD1057 pKa = 4.23DD1058 pKa = 3.62YY1059 pKa = 11.91LVGTTTASVFDD1070 pKa = 4.15GKK1072 pKa = 10.93GGNDD1076 pKa = 3.35FEE1078 pKa = 4.87TGGGKK1083 pKa = 10.5GDD1085 pKa = 3.32TFVFNAGYY1093 pKa = 9.82GHH1095 pKa = 7.08LEE1097 pKa = 3.9IYY1099 pKa = 9.18EE1100 pKa = 4.1YY1101 pKa = 9.92PYY1103 pKa = 10.97QPTNVLQLGPGISASSVEE1121 pKa = 4.29VSATEE1126 pKa = 4.08DD1127 pKa = 3.4YY1128 pKa = 11.41GVILTDD1134 pKa = 3.78GMAGDD1139 pKa = 4.71EE1140 pKa = 4.29IEE1142 pKa = 5.36LDD1144 pKa = 3.8GEE1146 pKa = 4.39LGGEE1150 pKa = 4.26GVEE1153 pKa = 4.01QVQFSDD1159 pKa = 3.48GTVWTRR1165 pKa = 11.84QQLLQMAEE1173 pKa = 4.39TTTGTPGDD1181 pKa = 4.16DD1182 pKa = 3.53VLLGTTDD1189 pKa = 3.41ASLFDD1194 pKa = 3.95GKK1196 pKa = 10.95GGNDD1200 pKa = 3.46YY1201 pKa = 11.6EE1202 pKa = 4.39EE1203 pKa = 5.86GDD1205 pKa = 4.26GNGHH1209 pKa = 7.5LDD1211 pKa = 3.29TFVFNAGYY1219 pKa = 9.93GHH1221 pKa = 7.63LEE1223 pKa = 3.8IDD1225 pKa = 3.46EE1226 pKa = 4.55SNYY1229 pKa = 9.7TVATSVLEE1237 pKa = 4.05LGPGISEE1244 pKa = 4.46SSLQVTTDD1252 pKa = 2.93KK1253 pKa = 11.64SEE1255 pKa = 5.36DD1256 pKa = 3.34IVLTSGTDD1264 pKa = 3.2QIVIDD1269 pKa = 4.21WMADD1273 pKa = 2.67GWGDD1277 pKa = 3.63YY1278 pKa = 11.07GVGQVTFADD1287 pKa = 4.17GTVWTASQIIAMASNVQGTAGNDD1310 pKa = 3.44TLYY1313 pKa = 9.57GTSISSTFDD1322 pKa = 3.02GKK1324 pKa = 11.04GGNDD1328 pKa = 3.29VEE1330 pKa = 4.66IGNGNDD1336 pKa = 3.12DD1337 pKa = 3.87TFIFDD1342 pKa = 3.78PGYY1345 pKa = 9.3GHH1347 pKa = 7.66LEE1349 pKa = 3.98IEE1351 pKa = 4.4EE1352 pKa = 4.28GMANYY1357 pKa = 10.64DD1358 pKa = 3.63STNVLQFGAGIRR1370 pKa = 11.84AAMVTASLGSNGSIVLTSGTDD1391 pKa = 3.43QVVLDD1396 pKa = 3.97GMTTQSCGIQLVQFADD1412 pKa = 4.14GTQWTPSQILAMASIIRR1429 pKa = 11.84GTAGNDD1435 pKa = 3.63TLSGTAGGDD1444 pKa = 3.05WFDD1447 pKa = 3.71GRR1449 pKa = 11.84GGNDD1453 pKa = 3.39LEE1455 pKa = 4.58VGGGGADD1462 pKa = 2.76TFVFNQGYY1470 pKa = 8.16GHH1472 pKa = 7.39LEE1474 pKa = 3.64IDD1476 pKa = 3.83EE1477 pKa = 4.23QDD1479 pKa = 3.56YY1480 pKa = 11.36SHH1482 pKa = 7.08GIVGKK1487 pKa = 8.32VLKK1490 pKa = 9.67LGPGIAPSSVTVTSDD1505 pKa = 2.7QYY1507 pKa = 11.58QDD1509 pKa = 3.34VVLTSGSDD1517 pKa = 3.46QIQLDD1522 pKa = 3.75GMADD1526 pKa = 3.35NSGLRR1531 pKa = 11.84GVQQVQFADD1540 pKa = 3.9GTLWTAAQMIAWARR1554 pKa = 11.84DD1555 pKa = 3.63VQGTPGNEE1563 pKa = 3.68TLYY1566 pKa = 9.5GTNGADD1572 pKa = 3.31IFDD1575 pKa = 4.24GQGGNDD1581 pKa = 3.18IEE1583 pKa = 4.7IGNGGADD1590 pKa = 2.76TFVFNQGYY1598 pKa = 8.21GQLEE1602 pKa = 3.89IDD1604 pKa = 3.8EE1605 pKa = 4.73ANYY1608 pKa = 10.65YY1609 pKa = 10.14PGTNSVLRR1617 pKa = 11.84LGAGIDD1623 pKa = 3.6EE1624 pKa = 5.03SEE1626 pKa = 4.46VSVAGTSSRR1635 pKa = 11.84GLVLTDD1641 pKa = 5.49GIAGDD1646 pKa = 5.45QITLDD1651 pKa = 3.44NALYY1655 pKa = 10.37GSDD1658 pKa = 3.61YY1659 pKa = 11.16GVQQVQFADD1668 pKa = 3.7GTTWSAQQLEE1678 pKa = 4.4QMATTGNAGGGTLYY1692 pKa = 8.34GTPGADD1698 pKa = 2.99VFDD1701 pKa = 4.16SRR1703 pKa = 11.84GGNATEE1709 pKa = 4.1VGNGGSDD1716 pKa = 3.4TYY1718 pKa = 11.34LLQPGYY1724 pKa = 11.22GPITIVNGISSNNVSEE1740 pKa = 4.47GVLSIAGVNPDD1751 pKa = 4.33NVWLAQVGNNLQVEE1765 pKa = 4.86VMGSTTEE1772 pKa = 3.8ATIQNWFNNSYY1783 pKa = 11.03SKK1785 pKa = 10.9LGEE1788 pKa = 4.06LTVSGGTAGNMTLDD1802 pKa = 3.42ARR1804 pKa = 11.84IDD1806 pKa = 3.67QLVQAMATFSSNNPGFDD1823 pKa = 3.67PTSSANPVINDD1834 pKa = 3.57PTLLTAVNSAWHH1846 pKa = 4.6QQ1847 pKa = 3.35

Molecular weight: 191.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N7X5M2|A0A2N7X5M2_9BURK Nuclear transport factor 2 family protein OS=Trinickia symbiotica OX=863227 GN=C0Z20_11780 PE=4 SV=1
MM1 pKa = 6.8TASARR6 pKa = 11.84TAGTASAGTTSGTASGTTGGTSGTASGTTGTTGTTGTTGTTSGTTSGTTSGTTSARR62 pKa = 11.84TAGARR67 pKa = 11.84TAGARR72 pKa = 11.84TAGARR77 pKa = 11.84TASARR82 pKa = 11.84TAGARR87 pKa = 11.84TASAGTASAGTTSGTTSARR106 pKa = 11.84TASTGTASTRR116 pKa = 11.84TASTRR121 pKa = 11.84TASTRR126 pKa = 11.84TASAASTRR134 pKa = 11.84TAAA137 pKa = 4.39

Molecular weight: 12.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5982

0

5982

1928138

23

3089

322.3

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.874 ± 0.046

0.96 ± 0.011

5.395 ± 0.023

5.436 ± 0.036

3.671 ± 0.019

8.208 ± 0.035

2.277 ± 0.016

4.838 ± 0.023

3.014 ± 0.029

10.056 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.312 ± 0.015

2.712 ± 0.022

5.092 ± 0.024

3.32 ± 0.023

7.246 ± 0.038

5.731 ± 0.028

5.336 ± 0.03

7.728 ± 0.024

1.356 ± 0.013

2.438 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski