Mycobacterium phage Phrappuccino

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DDS8|A0A514DDS8_9CAUD Uncharacterized protein OS=Mycobacterium phage Phrappuccino OX=2591223 GN=83 PE=4 SV=1
MM1 pKa = 7.86ADD3 pKa = 3.59FASNCPVSWPIKK15 pKa = 10.09IPNLLPEE22 pKa = 4.77IDD24 pKa = 4.07LNGVMFSYY32 pKa = 10.41QHH34 pKa = 5.91GLVYY38 pKa = 10.77NADD41 pKa = 4.3DD42 pKa = 4.67PPSQLRR48 pKa = 11.84QPVEE52 pKa = 3.86EE53 pKa = 4.22

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DDW0|A0A514DDW0_9CAUD Uncharacterized protein OS=Mycobacterium phage Phrappuccino OX=2591223 GN=128 PE=4 SV=1
MM1 pKa = 7.84GRR3 pKa = 11.84GQLMKK8 pKa = 10.86LEE10 pKa = 4.17FTIEE14 pKa = 4.15HH15 pKa = 6.27VSPEE19 pKa = 3.7EE20 pKa = 3.97DD21 pKa = 3.22LANRR25 pKa = 11.84AMSLVMGYY33 pKa = 7.29QQRR36 pKa = 11.84RR37 pKa = 11.84LGPRR41 pKa = 11.84QEE43 pKa = 4.29QMLLDD48 pKa = 4.1MAAHH52 pKa = 6.47GRR54 pKa = 11.84PGFFGDD60 pKa = 3.39SAANRR65 pKa = 11.84DD66 pKa = 3.29VLARR70 pKa = 11.84LEE72 pKa = 4.01RR73 pKa = 11.84RR74 pKa = 11.84GLVRR78 pKa = 11.84VQPGTPDD85 pKa = 3.84LQPGWAASRR94 pKa = 11.84PWRR97 pKa = 11.84LTKK100 pKa = 10.59FGKK103 pKa = 10.21AVAAALTTVRR113 pKa = 11.84SPHH116 pKa = 6.2DD117 pKa = 3.59HH118 pKa = 6.64SSS120 pKa = 2.84

Molecular weight: 13.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

201

0

201

44098

35

2030

219.4

24.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.282 ± 0.263

1.025 ± 0.075

7.168 ± 0.306

6.241 ± 0.176

2.984 ± 0.077

8.297 ± 0.249

2.386 ± 0.137

4.109 ± 0.152

3.288 ± 0.158

8.082 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.116

3.177 ± 0.116

5.844 ± 0.203

3.993 ± 0.154

6.585 ± 0.2

5.608 ± 0.159

6.476 ± 0.29

7.127 ± 0.246

2.011 ± 0.1

2.941 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski