Amino acid dipepetide frequency for Mycobacterium phage Phrappuccino

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.654AlaAla: 12.654 ± 0.765
0.93AlaCys: 0.93 ± 0.163
7.438AlaAsp: 7.438 ± 0.497
7.075AlaGlu: 7.075 ± 0.439
2.381AlaPhe: 2.381 ± 0.22
7.597AlaGly: 7.597 ± 0.529
2.2AlaHis: 2.2 ± 0.266
4.467AlaIle: 4.467 ± 0.432
3.492AlaLys: 3.492 ± 0.31
8.844AlaLeu: 8.844 ± 0.536
3.084AlaMet: 3.084 ± 0.302
3.402AlaAsn: 3.402 ± 0.296
5.465AlaPro: 5.465 ± 0.342
4.558AlaGln: 4.558 ± 0.337
6.327AlaArg: 6.327 ± 0.439
5.511AlaSer: 5.511 ± 0.357
5.851AlaThr: 5.851 ± 0.517
7.121AlaVal: 7.121 ± 0.542
1.792AlaTrp: 1.792 ± 0.237
2.631AlaTyr: 2.631 ± 0.275
0.0AlaXaa: 0.0 ± 0.0
Cys
0.952CysAla: 0.952 ± 0.14
0.181CysCys: 0.181 ± 0.067
0.794CysAsp: 0.794 ± 0.159
0.839CysGlu: 0.839 ± 0.154
0.317CysPhe: 0.317 ± 0.091
1.02CysGly: 1.02 ± 0.146
0.272CysHis: 0.272 ± 0.084
0.272CysIle: 0.272 ± 0.078
0.317CysLys: 0.317 ± 0.112
0.544CysLeu: 0.544 ± 0.135
0.272CysMet: 0.272 ± 0.094
0.204CysAsn: 0.204 ± 0.066
0.703CysPro: 0.703 ± 0.159
0.295CysGln: 0.295 ± 0.08
0.748CysArg: 0.748 ± 0.136
0.771CysSer: 0.771 ± 0.126
0.748CysThr: 0.748 ± 0.143
0.408CysVal: 0.408 ± 0.103
0.249CysTrp: 0.249 ± 0.084
0.34CysTyr: 0.34 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
6.735AspAla: 6.735 ± 0.448
0.658AspCys: 0.658 ± 0.117
5.737AspAsp: 5.737 ± 0.732
5.465AspGlu: 5.465 ± 0.485
2.563AspPhe: 2.563 ± 0.297
6.259AspGly: 6.259 ± 0.336
1.95AspHis: 1.95 ± 0.256
2.699AspIle: 2.699 ± 0.231
2.109AspLys: 2.109 ± 0.259
6.486AspLeu: 6.486 ± 0.363
1.61AspMet: 1.61 ± 0.265
1.973AspAsn: 1.973 ± 0.227
6.236AspPro: 6.236 ± 0.701
2.29AspGln: 2.29 ± 0.198
3.878AspArg: 3.878 ± 0.332
3.129AspSer: 3.129 ± 0.267
4.082AspThr: 4.082 ± 0.302
4.059AspVal: 4.059 ± 0.344
1.315AspTrp: 1.315 ± 0.26
2.449AspTyr: 2.449 ± 0.381
0.0AspXaa: 0.0 ± 0.0
Glu
5.828GluAla: 5.828 ± 0.413
0.476GluCys: 0.476 ± 0.114
4.966GluAsp: 4.966 ± 0.638
3.787GluGlu: 3.787 ± 0.388
1.882GluPhe: 1.882 ± 0.201
4.898GluGly: 4.898 ± 0.293
1.905GluHis: 1.905 ± 0.207
3.152GluIle: 3.152 ± 0.286
2.109GluLys: 2.109 ± 0.233
6.146GluLeu: 6.146 ± 0.355
1.315GluMet: 1.315 ± 0.194
1.383GluAsn: 1.383 ± 0.186
2.835GluPro: 2.835 ± 0.414
3.266GluGln: 3.266 ± 0.29
4.309GluArg: 4.309 ± 0.375
2.426GluSer: 2.426 ± 0.228
3.47GluThr: 3.47 ± 0.314
5.125GluVal: 5.125 ± 0.355
1.429GluTrp: 1.429 ± 0.218
1.701GluTyr: 1.701 ± 0.229
0.0GluXaa: 0.0 ± 0.0
Phe
3.243PheAla: 3.243 ± 0.268
0.476PheCys: 0.476 ± 0.111
2.971PheAsp: 2.971 ± 0.225
1.633PheGlu: 1.633 ± 0.385
0.748PhePhe: 0.748 ± 0.133
2.721PheGly: 2.721 ± 0.22
0.544PheHis: 0.544 ± 0.123
0.975PheIle: 0.975 ± 0.148
0.93PheLys: 0.93 ± 0.12
2.177PheLeu: 2.177 ± 0.208
0.68PheMet: 0.68 ± 0.118
1.043PheAsn: 1.043 ± 0.168
1.474PhePro: 1.474 ± 0.184
1.066PheGln: 1.066 ± 0.176
2.086PheArg: 2.086 ± 0.227
1.678PheSer: 1.678 ± 0.188
1.814PheThr: 1.814 ± 0.217
2.154PheVal: 2.154 ± 0.223
0.499PheTrp: 0.499 ± 0.099
0.93PheTyr: 0.93 ± 0.14
0.0PheXaa: 0.0 ± 0.0
Gly
7.438GlyAla: 7.438 ± 0.5
0.726GlyCys: 0.726 ± 0.143
5.715GlyAsp: 5.715 ± 0.418
4.74GlyGlu: 4.74 ± 0.348
2.676GlyPhe: 2.676 ± 0.258
8.073GlyGly: 8.073 ± 0.805
1.837GlyHis: 1.837 ± 0.193
3.447GlyIle: 3.447 ± 0.247
2.925GlyLys: 2.925 ± 0.237
6.44GlyLeu: 6.44 ± 0.337
2.109GlyMet: 2.109 ± 0.199
2.245GlyAsn: 2.245 ± 0.304
3.969GlyPro: 3.969 ± 0.348
3.674GlyGln: 3.674 ± 0.257
5.034GlyArg: 5.034 ± 0.45
5.216GlySer: 5.216 ± 0.39
6.123GlyThr: 6.123 ± 0.609
6.1GlyVal: 6.1 ± 0.298
1.95GlyTrp: 1.95 ± 0.243
2.54GlyTyr: 2.54 ± 0.254
0.0GlyXaa: 0.0 ± 0.0
His
1.837HisAla: 1.837 ± 0.222
0.249HisCys: 0.249 ± 0.076
1.701HisAsp: 1.701 ± 0.225
1.678HisGlu: 1.678 ± 0.193
0.544HisPhe: 0.544 ± 0.117
1.701HisGly: 1.701 ± 0.218
0.884HisHis: 0.884 ± 0.168
0.839HisIle: 0.839 ± 0.115
0.544HisLys: 0.544 ± 0.128
2.29HisLeu: 2.29 ± 0.258
0.431HisMet: 0.431 ± 0.095
0.794HisAsn: 0.794 ± 0.112
1.95HisPro: 1.95 ± 0.303
0.884HisGln: 0.884 ± 0.147
1.882HisArg: 1.882 ± 0.255
1.066HisSer: 1.066 ± 0.21
1.293HisThr: 1.293 ± 0.178
1.451HisVal: 1.451 ± 0.205
0.998HisTrp: 0.998 ± 0.158
0.839HisTyr: 0.839 ± 0.125
0.0HisXaa: 0.0 ± 0.0
Ile
4.626IleAla: 4.626 ± 0.352
0.544IleCys: 0.544 ± 0.116
3.628IleAsp: 3.628 ± 0.296
3.061IleGlu: 3.061 ± 0.308
0.952IlePhe: 0.952 ± 0.129
3.402IleGly: 3.402 ± 0.34
0.703IleHis: 0.703 ± 0.13
1.474IleIle: 1.474 ± 0.238
1.361IleLys: 1.361 ± 0.205
2.585IleLeu: 2.585 ± 0.254
0.658IleMet: 0.658 ± 0.104
1.474IleAsn: 1.474 ± 0.249
2.358IlePro: 2.358 ± 0.22
1.474IleGln: 1.474 ± 0.16
2.472IleArg: 2.472 ± 0.263
2.245IleSer: 2.245 ± 0.225
3.583IleThr: 3.583 ± 0.328
2.721IleVal: 2.721 ± 0.253
0.567IleTrp: 0.567 ± 0.145
1.202IleTyr: 1.202 ± 0.167
0.0IleXaa: 0.0 ± 0.0
Lys
3.742LysAla: 3.742 ± 0.35
0.159LysCys: 0.159 ± 0.06
2.358LysAsp: 2.358 ± 0.305
1.519LysGlu: 1.519 ± 0.219
1.134LysPhe: 1.134 ± 0.15
2.631LysGly: 2.631 ± 0.291
0.907LysHis: 0.907 ± 0.164
1.542LysIle: 1.542 ± 0.182
1.361LysLys: 1.361 ± 0.26
2.948LysLeu: 2.948 ± 0.243
0.862LysMet: 0.862 ± 0.164
1.134LysAsn: 1.134 ± 0.17
1.406LysPro: 1.406 ± 0.224
1.315LysGln: 1.315 ± 0.198
2.245LysArg: 2.245 ± 0.271
1.519LysSer: 1.519 ± 0.184
1.905LysThr: 1.905 ± 0.258
2.404LysVal: 2.404 ± 0.283
0.68LysTrp: 0.68 ± 0.131
1.111LysTyr: 1.111 ± 0.155
0.0LysXaa: 0.0 ± 0.0
Leu
8.753LeuAla: 8.753 ± 0.557
0.703LeuCys: 0.703 ± 0.156
6.644LeuAsp: 6.644 ± 0.532
4.377LeuGlu: 4.377 ± 0.313
2.109LeuPhe: 2.109 ± 0.223
6.508LeuGly: 6.508 ± 0.384
2.064LeuHis: 2.064 ± 0.199
3.039LeuIle: 3.039 ± 0.354
2.472LeuLys: 2.472 ± 0.264
6.032LeuLeu: 6.032 ± 0.406
1.61LeuMet: 1.61 ± 0.227
2.676LeuAsn: 2.676 ± 0.319
4.309LeuPro: 4.309 ± 0.316
3.583LeuGln: 3.583 ± 0.277
5.556LeuArg: 5.556 ± 0.341
5.329LeuSer: 5.329 ± 0.415
6.123LeuThr: 6.123 ± 0.561
5.125LeuVal: 5.125 ± 0.314
1.338LeuTrp: 1.338 ± 0.182
2.472LeuTyr: 2.472 ± 0.278
0.0LeuXaa: 0.0 ± 0.0
Met
3.197MetAla: 3.197 ± 0.259
0.204MetCys: 0.204 ± 0.059
0.794MetAsp: 0.794 ± 0.18
0.952MetGlu: 0.952 ± 0.157
0.612MetPhe: 0.612 ± 0.128
1.769MetGly: 1.769 ± 0.237
0.408MetHis: 0.408 ± 0.095
1.066MetIle: 1.066 ± 0.139
1.043MetLys: 1.043 ± 0.147
1.882MetLeu: 1.882 ± 0.188
0.544MetMet: 0.544 ± 0.106
0.771MetAsn: 0.771 ± 0.147
1.655MetPro: 1.655 ± 0.228
0.884MetGln: 0.884 ± 0.191
1.701MetArg: 1.701 ± 0.194
2.064MetSer: 2.064 ± 0.248
2.177MetThr: 2.177 ± 0.186
1.27MetVal: 1.27 ± 0.171
0.317MetTrp: 0.317 ± 0.082
0.431MetTyr: 0.431 ± 0.079
0.0MetXaa: 0.0 ± 0.0
Asn
2.744AsnAla: 2.744 ± 0.259
0.317AsnCys: 0.317 ± 0.091
1.95AsnAsp: 1.95 ± 0.259
1.678AsnGlu: 1.678 ± 0.162
1.406AsnPhe: 1.406 ± 0.189
2.812AsnGly: 2.812 ± 0.322
0.839AsnHis: 0.839 ± 0.139
1.429AsnIle: 1.429 ± 0.22
1.111AsnLys: 1.111 ± 0.163
2.2AsnLeu: 2.2 ± 0.339
0.476AsnMet: 0.476 ± 0.133
1.179AsnAsn: 1.179 ± 0.178
2.88AsnPro: 2.88 ± 0.309
1.429AsnGln: 1.429 ± 0.219
2.313AsnArg: 2.313 ± 0.275
1.86AsnSer: 1.86 ± 0.259
2.154AsnThr: 2.154 ± 0.259
1.814AsnVal: 1.814 ± 0.176
0.522AsnTrp: 0.522 ± 0.115
0.635AsnTyr: 0.635 ± 0.11
0.0AsnXaa: 0.0 ± 0.0
Pro
5.556ProAla: 5.556 ± 0.328
0.363ProCys: 0.363 ± 0.094
4.944ProAsp: 4.944 ± 0.5
4.876ProGlu: 4.876 ± 0.548
1.633ProPhe: 1.633 ± 0.192
5.397ProGly: 5.397 ± 0.471
1.383ProHis: 1.383 ± 0.199
2.109ProIle: 2.109 ± 0.212
1.882ProLys: 1.882 ± 0.254
4.127ProLeu: 4.127 ± 0.275
1.792ProMet: 1.792 ± 0.267
1.95ProAsn: 1.95 ± 0.247
3.515ProPro: 3.515 ± 0.315
1.95ProGln: 1.95 ± 0.27
3.084ProArg: 3.084 ± 0.272
3.356ProSer: 3.356 ± 0.304
3.356ProThr: 3.356 ± 0.292
4.49ProVal: 4.49 ± 0.335
0.884ProTrp: 0.884 ± 0.154
1.792ProTyr: 1.792 ± 0.244
0.0ProXaa: 0.0 ± 0.0
Gln
4.762GlnAla: 4.762 ± 0.377
0.499GlnCys: 0.499 ± 0.116
1.633GlnAsp: 1.633 ± 0.191
2.109GlnGlu: 2.109 ± 0.215
1.61GlnPhe: 1.61 ± 0.211
2.426GlnGly: 2.426 ± 0.25
0.884GlnHis: 0.884 ± 0.149
1.95GlnIle: 1.95 ± 0.193
1.293GlnLys: 1.293 ± 0.224
3.696GlnLeu: 3.696 ± 0.316
1.202GlnMet: 1.202 ± 0.189
0.93GlnAsn: 0.93 ± 0.158
2.018GlnPro: 2.018 ± 0.242
2.132GlnGln: 2.132 ± 0.277
3.016GlnArg: 3.016 ± 0.286
2.313GlnSer: 2.313 ± 0.272
1.973GlnThr: 1.973 ± 0.255
3.197GlnVal: 3.197 ± 0.387
1.179GlnTrp: 1.179 ± 0.158
1.111GlnTyr: 1.111 ± 0.144
0.0GlnXaa: 0.0 ± 0.0
Arg
6.35ArgAla: 6.35 ± 0.411
0.839ArgCys: 0.839 ± 0.174
3.81ArgAsp: 3.81 ± 0.351
3.991ArgGlu: 3.991 ± 0.359
2.109ArgPhe: 2.109 ± 0.198
4.581ArgGly: 4.581 ± 0.404
1.61ArgHis: 1.61 ± 0.202
2.835ArgIle: 2.835 ± 0.199
3.152ArgLys: 3.152 ± 0.261
4.83ArgLeu: 4.83 ± 0.398
1.633ArgMet: 1.633 ± 0.199
2.29ArgAsn: 2.29 ± 0.18
3.288ArgPro: 3.288 ± 0.289
2.563ArgGln: 2.563 ± 0.228
5.692ArgArg: 5.692 ± 0.525
3.061ArgSer: 3.061 ± 0.286
4.762ArgThr: 4.762 ± 0.297
4.762ArgVal: 4.762 ± 0.367
1.451ArgTrp: 1.451 ± 0.18
2.245ArgTyr: 2.245 ± 0.257
0.0ArgXaa: 0.0 ± 0.0
Ser
5.533SerAla: 5.533 ± 0.44
0.612SerCys: 0.612 ± 0.132
3.742SerAsp: 3.742 ± 0.375
3.175SerGlu: 3.175 ± 0.275
1.746SerPhe: 1.746 ± 0.224
6.078SerGly: 6.078 ± 0.526
1.134SerHis: 1.134 ± 0.16
2.404SerIle: 2.404 ± 0.272
1.655SerLys: 1.655 ± 0.194
3.878SerLeu: 3.878 ± 0.309
1.86SerMet: 1.86 ± 0.236
1.814SerAsn: 1.814 ± 0.206
3.288SerPro: 3.288 ± 0.303
1.973SerGln: 1.973 ± 0.281
3.084SerArg: 3.084 ± 0.268
3.787SerSer: 3.787 ± 0.365
3.107SerThr: 3.107 ± 0.311
4.286SerVal: 4.286 ± 0.454
1.247SerTrp: 1.247 ± 0.187
1.678SerTyr: 1.678 ± 0.175
0.0SerXaa: 0.0 ± 0.0
Thr
7.03ThrAla: 7.03 ± 0.491
0.59ThrCys: 0.59 ± 0.117
4.762ThrAsp: 4.762 ± 0.379
3.855ThrGlu: 3.855 ± 0.268
1.746ThrPhe: 1.746 ± 0.235
5.964ThrGly: 5.964 ± 0.465
1.474ThrHis: 1.474 ± 0.212
2.495ThrIle: 2.495 ± 0.304
1.814ThrLys: 1.814 ± 0.221
5.647ThrLeu: 5.647 ± 0.507
1.02ThrMet: 1.02 ± 0.171
2.222ThrAsn: 2.222 ± 0.305
4.694ThrPro: 4.694 ± 0.369
1.996ThrGln: 1.996 ± 0.22
3.129ThrArg: 3.129 ± 0.289
3.719ThrSer: 3.719 ± 0.346
4.626ThrThr: 4.626 ± 0.507
4.966ThrVal: 4.966 ± 0.479
1.315ThrTrp: 1.315 ± 0.179
1.701ThrTyr: 1.701 ± 0.193
0.0ThrXaa: 0.0 ± 0.0
Val
7.325ValAla: 7.325 ± 0.59
0.998ValCys: 0.998 ± 0.139
4.649ValAsp: 4.649 ± 0.323
4.74ValGlu: 4.74 ± 0.376
2.086ValPhe: 2.086 ± 0.221
5.579ValGly: 5.579 ± 0.372
1.542ValHis: 1.542 ± 0.219
2.948ValIle: 2.948 ± 0.294
1.95ValLys: 1.95 ± 0.216
5.375ValLeu: 5.375 ± 0.338
1.633ValMet: 1.633 ± 0.211
2.563ValAsn: 2.563 ± 0.242
3.855ValPro: 3.855 ± 0.335
2.948ValGln: 2.948 ± 0.344
4.898ValArg: 4.898 ± 0.394
4.331ValSer: 4.331 ± 0.407
4.649ValThr: 4.649 ± 0.42
5.76ValVal: 5.76 ± 0.467
1.179ValTrp: 1.179 ± 0.145
2.268ValTyr: 2.268 ± 0.305
0.0ValXaa: 0.0 ± 0.0
Trp
2.041TrpAla: 2.041 ± 0.202
0.249TrpCys: 0.249 ± 0.081
1.179TrpAsp: 1.179 ± 0.192
1.27TrpGlu: 1.27 ± 0.182
0.567TrpPhe: 0.567 ± 0.11
1.406TrpGly: 1.406 ± 0.192
0.612TrpHis: 0.612 ± 0.144
0.794TrpIle: 0.794 ± 0.14
0.816TrpLys: 0.816 ± 0.215
1.792TrpLeu: 1.792 ± 0.201
0.567TrpMet: 0.567 ± 0.105
0.726TrpAsn: 0.726 ± 0.123
0.998TrpPro: 0.998 ± 0.151
0.612TrpGln: 0.612 ± 0.127
1.701TrpArg: 1.701 ± 0.198
0.93TrpSer: 0.93 ± 0.172
1.293TrpThr: 1.293 ± 0.189
1.587TrpVal: 1.587 ± 0.17
0.431TrpTrp: 0.431 ± 0.096
0.544TrpTyr: 0.544 ± 0.117
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.472TyrAla: 2.472 ± 0.222
0.476TyrCys: 0.476 ± 0.098
2.268TyrAsp: 2.268 ± 0.261
1.587TyrGlu: 1.587 ± 0.226
1.02TyrPhe: 1.02 ± 0.161
2.154TyrGly: 2.154 ± 0.231
0.703TyrHis: 0.703 ± 0.139
1.157TyrIle: 1.157 ± 0.144
0.544TyrLys: 0.544 ± 0.13
2.903TyrLeu: 2.903 ± 0.305
0.363TyrMet: 0.363 ± 0.086
0.998TyrAsn: 0.998 ± 0.151
1.542TyrPro: 1.542 ± 0.212
1.043TyrGln: 1.043 ± 0.139
2.699TyrArg: 2.699 ± 0.261
1.769TyrSer: 1.769 ± 0.2
1.678TyrThr: 1.678 ± 0.239
2.472TyrVal: 2.472 ± 0.301
0.771TyrTrp: 0.771 ± 0.132
0.794TyrTyr: 0.794 ± 0.133
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 201 proteins (44098 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski