Actinomyces urogenitalis DSM 15434
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2394 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0W4F9|C0W4F9_9ACTO Uncharacterized protein OS=Actinomyces urogenitalis DSM 15434 OX=525246 GN=HMPREF0058_0753 PE=4 SV=1
MM1 pKa = 7.27 HH2 pKa = 7.59 AEE4 pKa = 3.77 DD5 pKa = 5.72 LYY7 pKa = 11.42 YY8 pKa = 11.12 LNAADD13 pKa = 3.58 DD14 pKa = 3.62 WARR17 pKa = 11.84 RR18 pKa = 11.84 DD19 pKa = 3.62 EE20 pKa = 4.8 IEE22 pKa = 5.51 AEE24 pKa = 4.2 DD25 pKa = 4.4 AEE27 pKa = 4.81 HH28 pKa = 6.88 EE29 pKa = 3.98 AAYY32 pKa = 9.64 WDD34 pKa = 4.16 DD35 pKa = 3.55 ADD37 pKa = 4.84 RR38 pKa = 11.84 AYY40 pKa = 10.14 EE41 pKa = 3.79 QHH43 pKa = 6.98 RR44 pKa = 11.84 DD45 pKa = 3.47 EE46 pKa = 5.83 RR47 pKa = 11.84 DD48 pKa = 2.89 EE49 pKa = 4.97 DD50 pKa = 5.15 LYY52 pKa = 11.69 DD53 pKa = 3.95 NDD55 pKa = 4.41 PYY57 pKa = 11.36 EE58 pKa = 4.98 GGEE61 pKa = 3.95 AAA63 pKa = 5.36
Molecular weight: 7.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.834
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|C0W4T8|C0W4T8_9ACTO CobQ/CobB/MinD/ParA nucleotide binding domain protein OS=Actinomyces urogenitalis DSM 15434 OX=525246 GN=HMPREF0058_0882 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.24 VHH17 pKa = 5.37 GFRR20 pKa = 11.84 TRR22 pKa = 11.84 MSTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AVLASRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.61 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LAAA45 pKa = 4.44
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2394
0
2394
768598
32
1906
321.1
34.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.387 ± 0.067
0.779 ± 0.014
5.719 ± 0.042
5.932 ± 0.054
2.561 ± 0.036
8.907 ± 0.042
2.102 ± 0.027
3.729 ± 0.038
2.041 ± 0.042
10.222 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.066 ± 0.021
1.793 ± 0.028
5.414 ± 0.04
3.467 ± 0.032
7.154 ± 0.06
5.935 ± 0.037
6.257 ± 0.041
8.925 ± 0.048
1.553 ± 0.028
2.058 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here