Apilactobacillus kunkeei

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Apilactobacillus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M9DB11|A0A0M9DB11_9LACO UDP-glucose 4-epimerase OS=Apilactobacillus kunkeei OX=148814 GN=RZ71_02200 PE=3 SV=1
MM1 pKa = 7.93IYY3 pKa = 10.26QEE5 pKa = 3.89FAKK8 pKa = 10.45FYY10 pKa = 11.19DD11 pKa = 3.65EE12 pKa = 6.05LFDD15 pKa = 3.68EE16 pKa = 4.58TMYY19 pKa = 10.89DD20 pKa = 2.98QWLSFVKK27 pKa = 10.83ANVSKK32 pKa = 10.84NDD34 pKa = 3.75SIMDD38 pKa = 3.9MACGTGRR45 pKa = 11.84LLALLAAQNYY55 pKa = 7.17NVCGMDD61 pKa = 3.82LSEE64 pKa = 6.29DD65 pKa = 3.55MLTLAEE71 pKa = 4.03EE72 pKa = 4.41HH73 pKa = 6.74LRR75 pKa = 11.84DD76 pKa = 4.37IKK78 pKa = 10.98PDD80 pKa = 3.28MPLIQGDD87 pKa = 3.85MTEE90 pKa = 4.49MEE92 pKa = 5.44GIPNFDD98 pKa = 4.49VITCFDD104 pKa = 4.47DD105 pKa = 3.73SLCYY109 pKa = 10.64LPDD112 pKa = 3.53QNILQTAFEE121 pKa = 4.41KK122 pKa = 10.96VYY124 pKa = 10.92EE125 pKa = 4.17HH126 pKa = 7.11LNDD129 pKa = 4.65DD130 pKa = 3.94GQFLFDD136 pKa = 5.43VITPYY141 pKa = 9.28QTDD144 pKa = 3.39VVYY147 pKa = 10.26PGYY150 pKa = 9.87MYY152 pKa = 10.82NYY154 pKa = 9.31HH155 pKa = 7.46DD156 pKa = 5.4DD157 pKa = 3.82SNAFMWQSYY166 pKa = 10.54LGDD169 pKa = 3.62EE170 pKa = 4.42DD171 pKa = 5.31HH172 pKa = 7.38SVEE175 pKa = 4.09HH176 pKa = 6.97DD177 pKa = 3.74LVFFKK182 pKa = 11.3YY183 pKa = 10.35NEE185 pKa = 4.51SINAYY190 pKa = 9.36DD191 pKa = 3.57QFSEE195 pKa = 3.78IHH197 pKa = 6.46LEE199 pKa = 3.78RR200 pKa = 11.84TYY202 pKa = 11.3KK203 pKa = 9.82VDD205 pKa = 3.91IYY207 pKa = 11.07IDD209 pKa = 3.65LLKK212 pKa = 11.02NAGFKK217 pKa = 10.21DD218 pKa = 3.29IKK220 pKa = 10.78VSANFGKK227 pKa = 10.4DD228 pKa = 3.22EE229 pKa = 4.19ITDD232 pKa = 3.48TTTRR236 pKa = 11.84WFFVCKK242 pKa = 10.31KK243 pKa = 10.09GG244 pKa = 3.98

Molecular weight: 28.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C2VWM8|A0A0C2VWM8_9LACO Uncharacterized protein OS=Apilactobacillus kunkeei OX=148814 GN=HW41_03610 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.16KK9 pKa = 8.22RR10 pKa = 11.84KK11 pKa = 8.74HH12 pKa = 5.48ARR14 pKa = 11.84THH16 pKa = 5.82GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.4VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.51KK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.9SLTVV44 pKa = 3.12

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1332

0

1332

462814

38

8349

347.5

38.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.499 ± 0.251

0.442 ± 0.019

6.808 ± 0.097

5.545 ± 0.123

4.209 ± 0.092

6.449 ± 0.088

1.988 ± 0.042

7.545 ± 0.147

7.612 ± 0.111

8.512 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.068

6.188 ± 0.141

3.229 ± 0.042

4.012 ± 0.113

3.308 ± 0.075

6.761 ± 0.107

5.618 ± 0.086

7.066 ± 0.096

0.785 ± 0.028

3.818 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski