Amino acid dipepetide frequency for Apilactobacillus kunkeei

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.37AlaAla: 6.37 ± 0.546
0.38AlaCys: 0.38 ± 0.034
4.749AlaAsp: 4.749 ± 0.228
3.917AlaGlu: 3.917 ± 0.135
3.023AlaPhe: 3.023 ± 0.119
5.13AlaGly: 5.13 ± 0.257
1.247AlaHis: 1.247 ± 0.055
5.821AlaIle: 5.821 ± 0.189
6.664AlaLys: 6.664 ± 0.26
6.238AlaLeu: 6.238 ± 0.159
1.847AlaMet: 1.847 ± 0.068
4.499AlaAsn: 4.499 ± 0.303
1.828AlaPro: 1.828 ± 0.068
3.539AlaGln: 3.539 ± 0.373
2.189AlaArg: 2.189 ± 0.08
4.775AlaSer: 4.775 ± 0.241
4.369AlaThr: 4.369 ± 0.221
4.954AlaVal: 4.954 ± 0.122
0.449AlaTrp: 0.449 ± 0.029
3.006AlaTyr: 3.006 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
0.333CysAla: 0.333 ± 0.03
0.043CysCys: 0.043 ± 0.01
0.287CysAsp: 0.287 ± 0.027
0.24CysGlu: 0.24 ± 0.024
0.197CysPhe: 0.197 ± 0.025
0.465CysGly: 0.465 ± 0.039
0.127CysHis: 0.127 ± 0.016
0.313CysIle: 0.313 ± 0.035
0.233CysLys: 0.233 ± 0.022
0.428CysLeu: 0.428 ± 0.035
0.104CysMet: 0.104 ± 0.014
0.203CysAsn: 0.203 ± 0.024
0.186CysPro: 0.186 ± 0.022
0.123CysGln: 0.123 ± 0.016
0.147CysArg: 0.147 ± 0.018
0.236CysSer: 0.236 ± 0.024
0.214CysThr: 0.214 ± 0.022
0.3CysVal: 0.3 ± 0.031
0.045CysTrp: 0.045 ± 0.01
0.192CysTyr: 0.192 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
5.655AspAla: 5.655 ± 0.472
0.236AspCys: 0.236 ± 0.023
5.13AspAsp: 5.13 ± 0.148
5.101AspGlu: 5.101 ± 0.161
3.053AspPhe: 3.053 ± 0.108
4.68AspGly: 4.68 ± 0.257
1.299AspHis: 1.299 ± 0.064
4.633AspIle: 4.633 ± 0.144
5.149AspLys: 5.149 ± 0.203
5.531AspLeu: 5.531 ± 0.164
1.683AspMet: 1.683 ± 0.074
4.082AspAsn: 4.082 ± 0.132
2.035AspPro: 2.035 ± 0.098
2.61AspGln: 2.61 ± 0.116
1.977AspArg: 1.977 ± 0.086
4.036AspSer: 4.036 ± 0.182
3.187AspThr: 3.187 ± 0.121
4.71AspVal: 4.71 ± 0.106
0.594AspTrp: 0.594 ± 0.038
2.703AspTyr: 2.703 ± 0.097
0.0AspXaa: 0.0 ± 0.0
Glu
3.941GluAla: 3.941 ± 0.105
0.188GluCys: 0.188 ± 0.021
3.682GluAsp: 3.682 ± 0.134
3.74GluGlu: 3.74 ± 0.153
2.42GluPhe: 2.42 ± 0.1
2.707GluGly: 2.707 ± 0.096
1.149GluHis: 1.149 ± 0.056
4.373GluIle: 4.373 ± 0.148
4.602GluLys: 4.602 ± 0.194
5.283GluLeu: 5.283 ± 0.204
1.886GluMet: 1.886 ± 0.084
3.375GluAsn: 3.375 ± 0.104
1.521GluPro: 1.521 ± 0.066
2.288GluGln: 2.288 ± 0.089
1.917GluArg: 1.917 ± 0.091
3.211GluSer: 3.211 ± 0.109
2.774GluThr: 2.774 ± 0.087
3.777GluVal: 3.777 ± 0.149
0.465GluTrp: 0.465 ± 0.033
2.154GluTyr: 2.154 ± 0.075
0.0GluXaa: 0.0 ± 0.0
Phe
3.038PheAla: 3.038 ± 0.106
0.218PheCys: 0.218 ± 0.022
3.168PheAsp: 3.168 ± 0.105
2.223PheGlu: 2.223 ± 0.101
1.917PhePhe: 1.917 ± 0.1
2.997PheGly: 2.997 ± 0.089
0.808PheHis: 0.808 ± 0.047
3.384PheIle: 3.384 ± 0.144
2.975PheLys: 2.975 ± 0.099
3.474PheLeu: 3.474 ± 0.146
1.087PheMet: 1.087 ± 0.051
2.872PheAsn: 2.872 ± 0.127
1.234PhePro: 1.234 ± 0.066
1.141PheGln: 1.141 ± 0.048
1.16PheArg: 1.16 ± 0.057
2.934PheSer: 2.934 ± 0.108
2.323PheThr: 2.323 ± 0.071
3.053PheVal: 3.053 ± 0.115
0.4PheTrp: 0.4 ± 0.037
1.681PheTyr: 1.681 ± 0.068
0.0PheXaa: 0.0 ± 0.0
Gly
4.419GlyAla: 4.419 ± 0.224
0.344GlyCys: 0.344 ± 0.029
3.645GlyAsp: 3.645 ± 0.101
3.124GlyGlu: 3.124 ± 0.118
3.131GlyPhe: 3.131 ± 0.136
3.989GlyGly: 3.989 ± 0.152
1.437GlyHis: 1.437 ± 0.059
5.512GlyIle: 5.512 ± 0.145
4.97GlyLys: 4.97 ± 0.132
5.728GlyLeu: 5.728 ± 0.151
1.785GlyMet: 1.785 ± 0.059
3.507GlyAsn: 3.507 ± 0.218
1.437GlyPro: 1.437 ± 0.06
2.599GlyGln: 2.599 ± 0.162
2.223GlyArg: 2.223 ± 0.08
4.244GlySer: 4.244 ± 0.149
3.922GlyThr: 3.922 ± 0.172
4.704GlyVal: 4.704 ± 0.104
0.56GlyTrp: 0.56 ± 0.046
3.215GlyTyr: 3.215 ± 0.26
0.0GlyXaa: 0.0 ± 0.0
His
1.325HisAla: 1.325 ± 0.058
0.121HisCys: 0.121 ± 0.018
1.318HisAsp: 1.318 ± 0.057
1.067HisGlu: 1.067 ± 0.063
1.005HisPhe: 1.005 ± 0.057
1.495HisGly: 1.495 ± 0.06
0.583HisHis: 0.583 ± 0.042
1.301HisIle: 1.301 ± 0.065
1.292HisLys: 1.292 ± 0.062
1.798HisLeu: 1.798 ± 0.082
0.48HisMet: 0.48 ± 0.037
1.005HisAsn: 1.005 ± 0.053
0.927HisPro: 0.927 ± 0.057
0.828HisGln: 0.828 ± 0.05
0.72HisArg: 0.72 ± 0.046
1.212HisSer: 1.212 ± 0.047
0.91HisThr: 0.91 ± 0.044
1.476HisVal: 1.476 ± 0.074
0.181HisTrp: 0.181 ± 0.025
0.834HisTyr: 0.834 ± 0.043
0.0HisXaa: 0.0 ± 0.0
Ile
5.706IleAla: 5.706 ± 0.158
0.521IleCys: 0.521 ± 0.039
5.62IleAsp: 5.62 ± 0.189
4.192IleGlu: 4.192 ± 0.159
3.105IlePhe: 3.105 ± 0.137
5.026IleGly: 5.026 ± 0.183
1.327IleHis: 1.327 ± 0.063
5.845IleIle: 5.845 ± 0.195
5.454IleLys: 5.454 ± 0.148
6.212IleLeu: 6.212 ± 0.227
1.871IleMet: 1.871 ± 0.086
4.989IleAsn: 4.989 ± 0.124
2.645IlePro: 2.645 ± 0.081
2.45IleGln: 2.45 ± 0.081
2.381IleArg: 2.381 ± 0.085
5.51IleSer: 5.51 ± 0.161
4.116IleThr: 4.116 ± 0.12
5.454IleVal: 5.454 ± 0.166
0.536IleTrp: 0.536 ± 0.036
2.489IleTyr: 2.489 ± 0.08
0.0IleXaa: 0.0 ± 0.0
Lys
5.659LysAla: 5.659 ± 0.331
0.199LysCys: 0.199 ± 0.025
5.024LysAsp: 5.024 ± 0.134
4.885LysGlu: 4.885 ± 0.17
2.459LysPhe: 2.459 ± 0.099
3.723LysGly: 3.723 ± 0.131
1.707LysHis: 1.707 ± 0.086
5.125LysIle: 5.125 ± 0.138
6.927LysLys: 6.927 ± 0.268
6.257LysLeu: 6.257 ± 0.2
2.705LysMet: 2.705 ± 0.135
4.756LysAsn: 4.756 ± 0.128
2.457LysPro: 2.457 ± 0.087
3.639LysGln: 3.639 ± 0.11
2.958LysArg: 2.958 ± 0.107
4.568LysSer: 4.568 ± 0.109
4.371LysThr: 4.371 ± 0.129
4.993LysVal: 4.993 ± 0.152
0.635LysTrp: 0.635 ± 0.045
3.075LysTyr: 3.075 ± 0.099
0.0LysXaa: 0.0 ± 0.0
Leu
6.476LeuAla: 6.476 ± 0.147
0.441LeuCys: 0.441 ± 0.035
5.525LeuAsp: 5.525 ± 0.155
3.993LeuGlu: 3.993 ± 0.149
3.686LeuPhe: 3.686 ± 0.155
5.663LeuGly: 5.663 ± 0.154
1.538LeuHis: 1.538 ± 0.066
6.787LeuIle: 6.787 ± 0.249
6.197LeuLys: 6.197 ± 0.203
7.297LeuLeu: 7.297 ± 0.254
2.161LeuMet: 2.161 ± 0.094
5.292LeuAsn: 5.292 ± 0.137
3.286LeuPro: 3.286 ± 0.101
2.815LeuGln: 2.815 ± 0.083
2.772LeuArg: 2.772 ± 0.107
6.819LeuSer: 6.819 ± 0.167
5.233LeuThr: 5.233 ± 0.138
5.959LeuVal: 5.959 ± 0.194
0.57LeuTrp: 0.57 ± 0.042
2.612LeuTyr: 2.612 ± 0.095
0.0LeuXaa: 0.0 ± 0.0
Met
2.04MetAla: 2.04 ± 0.075
0.112MetCys: 0.112 ± 0.016
1.772MetAsp: 1.772 ± 0.073
1.24MetGlu: 1.24 ± 0.062
0.97MetPhe: 0.97 ± 0.058
1.688MetGly: 1.688 ± 0.072
0.516MetHis: 0.516 ± 0.039
2.113MetIle: 2.113 ± 0.106
2.146MetLys: 2.146 ± 0.089
2.094MetLeu: 2.094 ± 0.089
0.817MetMet: 0.817 ± 0.052
1.688MetAsn: 1.688 ± 0.069
0.97MetPro: 0.97 ± 0.044
1.095MetGln: 1.095 ± 0.06
0.843MetArg: 0.843 ± 0.047
1.731MetSer: 1.731 ± 0.069
1.534MetThr: 1.534 ± 0.057
1.802MetVal: 1.802 ± 0.071
0.125MetTrp: 0.125 ± 0.02
0.752MetTyr: 0.752 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
4.678AsnAla: 4.678 ± 0.364
0.218AsnCys: 0.218 ± 0.024
4.738AsnAsp: 4.738 ± 0.238
3.494AsnGlu: 3.494 ± 0.106
2.012AsnPhe: 2.012 ± 0.073
4.559AsnGly: 4.559 ± 0.296
1.42AsnHis: 1.42 ± 0.063
3.986AsnIle: 3.986 ± 0.106
4.57AsnLys: 4.57 ± 0.128
4.367AsnLeu: 4.367 ± 0.12
1.312AsnMet: 1.312 ± 0.052
4.395AsnAsn: 4.395 ± 0.319
2.686AsnPro: 2.686 ± 0.163
3.111AsnGln: 3.111 ± 0.152
2.076AsnArg: 2.076 ± 0.073
3.863AsnSer: 3.863 ± 0.182
3.012AsnThr: 3.012 ± 0.168
4.477AsnVal: 4.477 ± 0.129
0.601AsnTrp: 0.601 ± 0.042
2.31AsnTyr: 2.31 ± 0.1
0.0AsnXaa: 0.0 ± 0.0
Pro
2.359ProAla: 2.359 ± 0.115
0.102ProCys: 0.102 ± 0.014
2.245ProAsp: 2.245 ± 0.081
2.288ProGlu: 2.288 ± 0.093
1.502ProPhe: 1.502 ± 0.076
2.02ProGly: 2.02 ± 0.086
0.564ProHis: 0.564 ± 0.04
2.595ProIle: 2.595 ± 0.087
2.262ProLys: 2.262 ± 0.089
2.625ProLeu: 2.625 ± 0.103
0.694ProMet: 0.694 ± 0.045
1.953ProAsn: 1.953 ± 0.081
0.432ProPro: 0.432 ± 0.029
1.193ProGln: 1.193 ± 0.059
0.916ProArg: 0.916 ± 0.053
2.316ProSer: 2.316 ± 0.109
2.012ProThr: 2.012 ± 0.086
2.716ProVal: 2.716 ± 0.113
0.266ProTrp: 0.266 ± 0.024
1.225ProTyr: 1.225 ± 0.051
0.0ProXaa: 0.0 ± 0.0
Gln
3.444GlnAla: 3.444 ± 0.368
0.089GlnCys: 0.089 ± 0.016
2.092GlnAsp: 2.092 ± 0.096
1.88GlnGlu: 1.88 ± 0.072
1.666GlnPhe: 1.666 ± 0.063
2.197GlnGly: 2.197 ± 0.159
0.711GlnHis: 0.711 ± 0.043
2.742GlnIle: 2.742 ± 0.094
3.213GlnLys: 3.213 ± 0.15
3.816GlnLeu: 3.816 ± 0.112
1.074GlnMet: 1.074 ± 0.054
2.539GlnAsn: 2.539 ± 0.155
1.327GlnPro: 1.327 ± 0.063
1.992GlnGln: 1.992 ± 0.149
1.512GlnArg: 1.512 ± 0.071
3.008GlnSer: 3.008 ± 0.197
2.377GlnThr: 2.377 ± 0.147
2.388GlnVal: 2.388 ± 0.072
0.318GlnTrp: 0.318 ± 0.029
1.737GlnTyr: 1.737 ± 0.064
0.0GlnXaa: 0.0 ± 0.0
Arg
1.994ArgAla: 1.994 ± 0.072
0.162ArgCys: 0.162 ± 0.02
2.066ArgAsp: 2.066 ± 0.084
1.977ArgGlu: 1.977 ± 0.096
1.523ArgPhe: 1.523 ± 0.069
2.083ArgGly: 2.083 ± 0.085
0.715ArgHis: 0.715 ± 0.046
2.556ArgIle: 2.556 ± 0.107
2.478ArgLys: 2.478 ± 0.09
3.103ArgLeu: 3.103 ± 0.111
0.959ArgMet: 0.959 ± 0.05
1.83ArgAsn: 1.83 ± 0.076
1.16ArgPro: 1.16 ± 0.055
1.37ArgGln: 1.37 ± 0.058
1.623ArgArg: 1.623 ± 0.077
1.845ArgSer: 1.845 ± 0.071
1.7ArgThr: 1.7 ± 0.064
2.366ArgVal: 2.366 ± 0.09
0.233ArgTrp: 0.233 ± 0.026
1.34ArgTyr: 1.34 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
5.127SerAla: 5.127 ± 0.313
0.229SerCys: 0.229 ± 0.024
5.121SerAsp: 5.121 ± 0.201
3.451SerGlu: 3.451 ± 0.112
3.079SerPhe: 3.079 ± 0.106
4.455SerGly: 4.455 ± 0.173
1.411SerHis: 1.411 ± 0.063
5.153SerIle: 5.153 ± 0.157
4.706SerLys: 4.706 ± 0.115
5.754SerLeu: 5.754 ± 0.169
1.573SerMet: 1.573 ± 0.068
4.2SerAsn: 4.2 ± 0.189
1.744SerPro: 1.744 ± 0.066
2.733SerGln: 2.733 ± 0.164
2.139SerArg: 2.139 ± 0.082
5.449SerSer: 5.449 ± 0.326
3.794SerThr: 3.794 ± 0.141
4.468SerVal: 4.468 ± 0.113
0.499SerTrp: 0.499 ± 0.037
2.528SerTyr: 2.528 ± 0.09
0.0SerXaa: 0.0 ± 0.0
Thr
4.073ThrAla: 4.073 ± 0.208
0.214ThrCys: 0.214 ± 0.023
3.786ThrAsp: 3.786 ± 0.168
2.442ThrGlu: 2.442 ± 0.089
2.388ThrPhe: 2.388 ± 0.084
4.211ThrGly: 4.211 ± 0.187
1.063ThrHis: 1.063 ± 0.048
4.429ThrIle: 4.429 ± 0.133
4.185ThrLys: 4.185 ± 0.147
4.613ThrLeu: 4.613 ± 0.124
1.165ThrMet: 1.165 ± 0.057
3.451ThrAsn: 3.451 ± 0.189
2.435ThrPro: 2.435 ± 0.125
2.018ThrGln: 2.018 ± 0.11
1.579ThrArg: 1.579 ± 0.068
3.915ThrSer: 3.915 ± 0.166
3.623ThrThr: 3.623 ± 0.219
4.203ThrVal: 4.203 ± 0.138
0.443ThrTrp: 0.443 ± 0.035
1.94ThrTyr: 1.94 ± 0.084
0.0ThrXaa: 0.0 ± 0.0
Val
5.346ValAla: 5.346 ± 0.117
0.374ValCys: 0.374 ± 0.029
5.06ValAsp: 5.06 ± 0.14
3.939ValGlu: 3.939 ± 0.146
2.768ValPhe: 2.768 ± 0.099
4.447ValGly: 4.447 ± 0.123
1.26ValHis: 1.26 ± 0.065
5.657ValIle: 5.657 ± 0.182
5.145ValLys: 5.145 ± 0.114
6.136ValLeu: 6.136 ± 0.196
1.633ValMet: 1.633 ± 0.068
4.347ValAsn: 4.347 ± 0.114
2.414ValPro: 2.414 ± 0.11
2.396ValGln: 2.396 ± 0.093
2.094ValArg: 2.094 ± 0.082
5.026ValSer: 5.026 ± 0.115
4.157ValThr: 4.157 ± 0.155
5.516ValVal: 5.516 ± 0.149
0.493ValTrp: 0.493 ± 0.04
2.457ValTyr: 2.457 ± 0.093
0.0ValXaa: 0.0 ± 0.0
Trp
0.445TrpAla: 0.445 ± 0.032
0.037TrpCys: 0.037 ± 0.01
0.432TrpAsp: 0.432 ± 0.035
0.309TrpGlu: 0.309 ± 0.026
0.406TrpPhe: 0.406 ± 0.033
0.475TrpGly: 0.475 ± 0.035
0.212TrpHis: 0.212 ± 0.021
0.754TrpIle: 0.754 ± 0.055
0.475TrpLys: 0.475 ± 0.033
0.834TrpLeu: 0.834 ± 0.056
0.268TrpMet: 0.268 ± 0.028
0.439TrpAsn: 0.439 ± 0.033
0.225TrpPro: 0.225 ± 0.025
0.298TrpGln: 0.298 ± 0.03
0.313TrpArg: 0.313 ± 0.029
0.527TrpSer: 0.527 ± 0.036
0.421TrpThr: 0.421 ± 0.033
0.57TrpVal: 0.57 ± 0.042
0.093TrpTrp: 0.093 ± 0.018
0.318TrpTyr: 0.318 ± 0.031
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.567TyrAla: 2.567 ± 0.132
0.19TyrCys: 0.19 ± 0.018
2.625TyrAsp: 2.625 ± 0.098
1.951TyrGlu: 1.951 ± 0.067
1.778TyrPhe: 1.778 ± 0.075
2.483TyrGly: 2.483 ± 0.082
0.782TyrHis: 0.782 ± 0.055
2.368TyrIle: 2.368 ± 0.07
2.48TyrLys: 2.48 ± 0.116
3.535TyrLeu: 3.535 ± 0.112
0.942TyrMet: 0.942 ± 0.05
2.465TyrAsn: 2.465 ± 0.261
1.337TyrPro: 1.337 ± 0.066
1.88TyrGln: 1.88 ± 0.141
1.545TyrArg: 1.545 ± 0.061
2.39TyrSer: 2.39 ± 0.088
2.126TyrThr: 2.126 ± 0.107
2.776TyrVal: 2.776 ± 0.082
0.346TyrTrp: 0.346 ± 0.035
1.61TyrTyr: 1.61 ± 0.075
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1332 proteins (462814 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski