Ignicoccus islandicus DSM 13165
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1510 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U3F8T5|A0A0U3F8T5_9CREN TGT domain-containing protein OS=Ignicoccus islandicus DSM 13165 OX=940295 GN=EYM_03435 PE=4 SV=1
MM1 pKa = 8.31 DD2 pKa = 5.83 DD3 pKa = 4.18 LRR5 pKa = 11.84 LALVIGLSVGLANLFISIISGNLFLLTDD33 pKa = 4.32 LGALIEE39 pKa = 4.16 TLLLYY44 pKa = 10.51 SALAFALAYY53 pKa = 10.39 LSIYY57 pKa = 9.85 SSSIICEE64 pKa = 4.05 WVGACC69 pKa = 5.39
Molecular weight: 7.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.946
IPC2_protein 3.834
IPC_protein 3.528
Toseland 3.401
ProMoST 3.808
Dawson 3.579
Bjellqvist 3.77
Wikipedia 3.605
Rodwell 3.427
Grimsley 3.338
Solomon 3.478
Lehninger 3.427
Nozaki 3.783
DTASelect 3.872
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.681
Patrickios 0.006
IPC_peptide 3.465
IPC2_peptide 3.63
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A0U2M979|A0A0U2M979_9CREN Dihydroxy-acid dehydratase OS=Ignicoccus islandicus DSM 13165 OX=940295 GN=ilvD PE=3 SV=1
MM1 pKa = 7.22 GRR3 pKa = 11.84 KK4 pKa = 9.06 KK5 pKa = 10.74 VVGIAGRR12 pKa = 11.84 FGARR16 pKa = 11.84 YY17 pKa = 9.73 GSTLRR22 pKa = 11.84 KK23 pKa = 8.68 RR24 pKa = 11.84 WKK26 pKa = 9.66 QIMEE30 pKa = 3.94 ARR32 pKa = 11.84 YY33 pKa = 9.48 APHH36 pKa = 6.76 TCPICGMKK44 pKa = 10.39 GYY46 pKa = 9.57 VVRR49 pKa = 11.84 VSVGLWKK56 pKa = 10.42 CKK58 pKa = 9.91 KK59 pKa = 9.82 CGAVFAGAAYY69 pKa = 10.53 VPVSGLNKK77 pKa = 9.39 AYY79 pKa = 10.48 HH80 pKa = 5.9 MKK82 pKa = 10.25 PEE84 pKa = 3.83 RR85 pKa = 11.84 RR86 pKa = 11.84 RR87 pKa = 3.47
Molecular weight: 9.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 10.072
IPC_protein 10.804
Toseland 10.921
ProMoST 10.818
Dawson 11.008
Bjellqvist 10.716
Wikipedia 11.213
Rodwell 11.345
Grimsley 11.052
Solomon 11.125
Lehninger 11.096
Nozaki 10.906
DTASelect 10.701
Thurlkill 10.906
EMBOSS 11.316
Sillero 10.935
Patrickios 11.082
IPC_peptide 11.125
IPC2_peptide 9.838
IPC2.peptide.svr19 8.22
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1510
0
1510
415030
29
3141
274.9
30.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.875 ± 0.067
1.028 ± 0.031
4.558 ± 0.052
8.177 ± 0.087
3.571 ± 0.037
6.775 ± 0.056
1.46 ± 0.025
7.191 ± 0.06
7.538 ± 0.065
10.789 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.258 ± 0.028
3.518 ± 0.048
4.558 ± 0.042
1.752 ± 0.028
5.418 ± 0.057
6.161 ± 0.065
4.452 ± 0.07
8.762 ± 0.055
1.302 ± 0.024
3.858 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here