Pseudomonas kuykendallii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4331 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W5ERA3|A0A2W5ERA3_9PSED Bifunctional protein FolD OS=Pseudomonas kuykendallii OX=1007099 GN=folD PE=3 SV=1
MM1 pKa = 7.56KK2 pKa = 10.16KK3 pKa = 8.28WQCVVCGLIYY13 pKa = 10.71DD14 pKa = 4.34EE15 pKa = 5.33AEE17 pKa = 3.76GWPDD21 pKa = 3.48DD22 pKa = 4.63GIAAGTLWQDD32 pKa = 3.46VPEE35 pKa = 5.1DD36 pKa = 4.13WLCPDD41 pKa = 4.25CGVGKK46 pKa = 10.46SDD48 pKa = 4.34FEE50 pKa = 4.49MIEE53 pKa = 3.92IAA55 pKa = 5.16

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W5CX60|A0A2W5CX60_9PSED DUF484 domain-containing protein OS=Pseudomonas kuykendallii OX=1007099 GN=DI599_17810 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.34RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4331

0

4331

1397955

29

5871

322.8

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.629 ± 0.047

0.988 ± 0.01

5.441 ± 0.03

5.961 ± 0.04

3.625 ± 0.027

8.212 ± 0.051

2.19 ± 0.019

4.548 ± 0.027

3.104 ± 0.034

12.119 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.018

2.747 ± 0.027

4.912 ± 0.031

4.272 ± 0.026

7.067 ± 0.043

5.644 ± 0.035

4.489 ± 0.034

6.975 ± 0.03

1.403 ± 0.017

2.455 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski