Burkholderia sp. Ch1-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; unclassified Burkholderia

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7680 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2ITL6|I2ITL6_9BURK Transcriptional regulator of sugar metabolism OS=Burkholderia sp. Ch1-1 OX=243261 GN=BCh11DRAFT_01864 PE=4 SV=1
MM1 pKa = 7.03EE2 pKa = 4.92TLAQPAAATSGALPALLEE20 pKa = 4.14FTPQAAAKK28 pKa = 8.83VASLIEE34 pKa = 4.23EE35 pKa = 4.21EE36 pKa = 4.73GNPNLKK42 pKa = 10.22LRR44 pKa = 11.84LYY46 pKa = 10.67VSGGGCSGFQYY57 pKa = 10.88GFAFDD62 pKa = 5.01DD63 pKa = 3.81QVADD67 pKa = 5.01DD68 pKa = 5.31DD69 pKa = 4.42MQTVTDD75 pKa = 4.79GVTLLVDD82 pKa = 3.96SMSLQYY88 pKa = 11.1LPGARR93 pKa = 11.84VDD95 pKa = 3.98YY96 pKa = 11.02EE97 pKa = 4.51DD98 pKa = 4.21GLEE101 pKa = 3.97GSRR104 pKa = 11.84FVIHH108 pKa = 6.52NPNAQSTCGCGSSFSVV124 pKa = 3.54

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2IJI6|I2IJI6_9BURK Uncharacterized protein OS=Burkholderia sp. Ch1-1 OX=243261 GN=BCh11DRAFT_06400 PE=4 SV=1
MM1 pKa = 7.23QSQIMMMGFLAVSTLVTIAMFGRR24 pKa = 11.84TPQRR28 pKa = 11.84RR29 pKa = 11.84AVLKK33 pKa = 9.83RR34 pKa = 11.84ATVRR38 pKa = 11.84RR39 pKa = 11.84GGQQ42 pKa = 2.87

Molecular weight: 4.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7680

0

7680

2472543

20

3239

321.9

34.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.585 ± 0.041

0.932 ± 0.01

5.351 ± 0.021

5.163 ± 0.027

3.691 ± 0.019

8.117 ± 0.029

2.336 ± 0.013

4.706 ± 0.02

3.143 ± 0.024

10.21 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.014

2.877 ± 0.024

5.059 ± 0.024

3.715 ± 0.019

6.953 ± 0.035

5.8 ± 0.026

5.469 ± 0.024

7.704 ± 0.021

1.379 ± 0.011

2.462 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski